Cloning of genes and cells. Medical search. Frequent questions

The Mucin 1 (MUC1) gene encodes a membrane bound glycosylated phosphoprotein, belonging to the family of mucins which are heavily glycosylated proteins. (sigmaaldrich.com)
Extensive evidence has revealed that the core clock machinery involves "clock genes" and "clock proteins" functioning as molecular cogs. (bioone.org)
A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. (origene.com)
α-crystallin is a member of the small heat shock protein family, which helps in maintaining and thickening of the cell wall and in providing stability to proteins that allow the bacteria to survive under harsher conditions 7 . (nature.com)
Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. (nhbs.com)
These findings suggest that while channel proteins found in sperm cells from different species look similar and serve similar roles, they are activated in ways that can be very different. (elifesciences.org)
Lectins, the carbohydrate-binding proteins, are capable of binding to receptors present on the target cell/tissue and interact with transformed glycans better than normal cells. (bvsalud.org)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)

Researchers hope to use these cells to grow healthy tissue to replace injured or diseased tissues in the human body. (medlineplus.gov)

Based on the nucleotide sequence of the gene, it was deduced that the protein comprises 165 amino acids and that the molecular weight is 18 779. (elsevierpure.com)
Expression vectors are designed for expressing the protein encoded by the inserted gene. (biologyexams4u.com)
Cosmid can be defined as the hybrid vectors derived from plasmids that contain cos site of phage which is essential for packaging of nucleic acid into protein coat plus essential features of plasmids and several unique restriction sites for the insertion of DNA to be cloned. (biologyexams4u.com)
Cloning and sequencing of the pancreatic islet neogenesis associated protein (INGAP) gene and its expression in islet neogenesis in hamsters. (jci.org)
Islet neogenesis associated protein (INGAP) is a product of a novel gene expressed in regenerating hamster pancreas. (jci.org)
This data suggests that the INGAP gene is a novel pancreatic gene expressed during islet neogenesis whose protein product is a constituent of Ilotropin and is capable of initiating duct cell proliferation, a prerequisite for islet neogenesis. (jci.org)
The specificity of T-cells is determined by the T-cell receptor (TCR), a heterodimer of alpha and beta protein chains. (elifesciences.org)
In serotype 4b, gtcA appears to be the first member of a bicistronic operon which includes a gene with homology to Bacillus subtilis rpmE , encoding ribosomal protein L31. (asm.org)
Topics covered will include cell membrane structure and function, metabolism, cell motility and division, genome structure and replication, the regulation of gene expression and protein production, genotype to phenotype relationship, and basic principles of inheritance. (middlebury.edu)
A splicing mutation in the Sik3 protein kinase gene causes a profound decrease in total wake time, owing to an increase in inherent sleep need. (nature.com)
Repression of Pumilio Protein Expression by Rbfox1 Promotes Germ Cell Differentiation. (tcd.ie)
We describe the purification of telomerase and the cloning of cDNAs encoding two protein subunits from the ciliate Tetrahymena. (jhu.edu)
Reprinted from Cell 81, Collins, Kathleen, et al, "Purification of Tetrahymena Telomerase and Cloning of Genes Encoding the Two Protein Components of the Enzyme", pg. (jhu.edu)
XLRS1 encodes a 224 amino acid protein called retinoschisin, which is expressed in photoreceptor and bipolar cells. (medscape.com)
Retinoschisin is a secreted protein found throughout the retina and is involved in intercellular adhesion, retinal cellular organization, cell-cell interactions within the inner nuclear layer, as well as synaptic connection between photoreceptors and bipolar cells. (medscape.com)
Here, we report that the recently sequenced genome from the red flour beetle Tribolium castaneum contains a gene coding for an oxytocin/vasopressin-like peptide, identical to the locust peptide, which we named inotocin (for insect oxytocin/vasopressin-like peptide) and a gene coding for an inotocin G protein-coupled. (lu.se)
Identified gene candidates will be studied further in the context of determining sensitivity of protein degrader drugs which are currently emerging as alternative therapies for leukemia. (lu.se)
The lectures will cover the current status of cell-based protein production systems, and theoretical aspects of the methodology. (lu.se)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
The frameshift mutation results in the formation of a truncated protein which cannot fold properly, promoting aggregation, and subsequent deposition in tubular cells. (medscape.com)

One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
For the most part, cloning is achieved through a process known as somatic cell nuclear transfer (SCNT). (syfy.com)
Before the announcement in February 1997 of the cloning of a sheep by somatic cell nuclear transfer, existing legislation in a number of countries already precluded human cloning for reproductive purposes, sometimes implicitly. (who.int)

Therapeutic cloning, which creates embryonic stem cells . (medlineplus.gov)
The resulting embryo was then used to create embryonic stem cells, capable of generating every cell type in the body, and the nuclei of these cells were injected into other eggs to produce clones. (newscientist.com)
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. (genscript.com)
SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
Embryonic stem cells are immortal, and have the potential to develop into any type of adult cell, even after months growing in culture dishes. (news-medical.net)
In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
Dermal fibroblasts were taken from a 35-year old male and a 75-year-old male and used to create embryonic stem cells. (news-medical.net)
Although attempts have not yet been made to create a therapeutic transplant from embryonic stem cells, the methods have been developed to allow the creation of functional, mature cells using human cell cloning technology. (news-medical.net)

assigns single-cell RNA-seq expression to cancer clones by probabilistically mapping RNA-seq to clone-specific copy number profiles using reparametrization gradient variational inference . (github.com)
assay as gene expression input. (github.com)
Kodama, KI, Nakabeppu, Y & Sekiguchi, M 1989, ' Cloning and expression of the Bacillus subtilis methyltransferase gene in Escherichia coli ada - cells ', Mutation Research-DNA Repair , vol. 218, no. 2, pp. 153-163. (elsevierpure.com)
Scientists armed with whole genome sequence data can now ask questions about genome structure and organization that impact gene expression. (genengnews.com)
Improved access to bioinformatics tools enables scientists to process, manage, and store raw data, annotate sequences to define genes, and quantitate RNA sequence data to get insight into gene expression patterns. (genengnews.com)
Amy Radunz, a nutritionist from the department of animal sciences at the University of Wisconsin in River Falls, has partnered with molecular biologist Hasan Khatib, an associate professor at University of Wisconsin-Madison, to ask how differences in maternal diet during pregnancy induce gene expression and DNA methylation changes in fetal tissues in sheep. (genengnews.com)
The most important requirement for recombinant DNA technology is the cloning vector and expression vectors. (biologyexams4u.com)
Although its first products focused on gene therapy, the company now produces tools for innate immunity research, immunology research, cancer research, RNA interference, cell culture, cloning and gene expression. (wikipedia.org)
The molecular mechanisms involved in the specific regulation of PDGF alpha R expression are unknown, but transcriptional regulation of the PDGF alpha R gene is most likely to be involved. (nih.gov)
This 5' flanking region is a functional promoter of the PDGF alpha R gene as concluded from its capacity to drive luciferase reporter gene expression in an orientation dependent way. (nih.gov)
Cloning, expression, and differentiation-dependent regulation of SMAF1 in adipogenesis. (nih.gov)
In addition, our expression data do not provide strong evidence that Grhl-3 is indeed the ct gene. (eur.nl)
Targeted disruption of the gene encoding D4Rs reduces the amplitude of the contrast sensitivity rhythm by reducing daytime sensitivity and abolishes the rhythmic expression of Npas2 and Adcy1 mRNA in the ganglion cell layer (GCL) of the retina. (jneurosci.org)
In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons. (origene.com)
Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. (addgene.org)
Title: Expression level of pre-B-cell leukemia transcription factor 2 (PBX2) as a prognostic marker for gingival squamous cell carcinoma. (genscript.com)
ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
GXD's primary emphasis is on endogenous gene expression during development. (jax.org)
The genetic structure includes 4 zinc-finger regions, which suggest that WT1 may be important in controlling the expression of other genes. (medscape.com)
A brief introduction to high throughput technologies for measuring and analyzing gene expression is given. (lu.se)
The main emphasis is on supervised machine learning methods for classification and prediction of tumor gene expression profiles. (lu.se)
Using such probes, one can obtain a fingerprint of the gene expression activity in a macroscopic sample. (lu.se)
Microarrays are one such tool that allows for the study of expression of thousands of genes simultaneously. (lu.se)
In what follows we limit ourselves to the analysis of gene expression measurements obtained using microarrays with small spots of DNA fixed to glass slides. (lu.se)
Levels of gene expression are measured using a preparation of fluorescently labeled tissue RNA (copies of DNA), together with reference RNA labeled with a different fluorochrome, hybridized onto the slides. (lu.se)
UMOD risk variants identified in the above-mentioned GWAS are located in the promoter region of the gene, leading to a theory that they altered UMOD expression. (medscape.com)

The approach was applied to five different hybridomas producing human monoclonal antibodies and variable regions for both bold gamma and mu heavy chain and kappa and lambda light chain genes were successfully cloned. (lu.se)
Dolly the sheep made headlines way back in 1996, only three years after Jurassic Park hit theaters, when she became the first successfully cloned mammal. (syfy.com)

Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. (elifesciences.org)
Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
These genes were selected based on their role in asthmatic inflammatory processes and History previously reported associations with asthma phenotypes. (cdc.gov)
Of these mediators, cytokines play a single nucleotide polymorphisms (SNP) in pro-inflammatory cytokine genes and asthma phenotypes (Che et al. (cdc.gov)

It enables researchers to search a genetic "library" of resistance genes discovered in wild relatives of modern crops so they can rapidly identify sequences associated with disease fighting capability. (phys.org)
By correlating this information with the DNA sequences of the plants, they were able to reveal the identity of functional resistance genes across the population. (phys.org)
We used VGF-homologous and TK gene nucleotide sequences to construct a phylogenetic tree for comparison with other poxviruses. (cdc.gov)
Gene sequences showed 99% homology with vaccinia virus genes and were clustered together with the isolated virus in the phylogenetic tree. (cdc.gov)
The following PBX2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)
[ 2 ] The product of the WT1 gene has zinc finger arrays that bind to specific DNA sequences, whereas the amino terminus appears to regulate transcription. (medscape.com)

InvivoGen is a provider of Toll-like receptor related products (mainly ligands and engineered mammalian cell lines), selection antibiotics and mycoplasma detection & elimination products. (wikipedia.org)
The Cell Culture Facility (CCF) located in the Life Science Laboratories supports research by providing space, equipment, cell cultures (plant and mammalian), media, supplies and training on a recharge basis for both academic labs and corporate partners. (umass.edu)

2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
These preparatory interregional and interdisciplinary meetings focused on the following areas: cloning and human reproductive health, biologicals, organ transplantation, research, and medical genetics. (who.int)
The main objection to the use of human cloning for reproductive purposes is that it would be contrary to human dignity as it would violate the uniqueness and indeterminateness of the human being. (who.int)
Some, however, consider that reproductive cloning could be acceptable in certain cases, such as otherwise untreatable infertility, or to avoid inherited genetic diseases. (who.int)
In terms of existing ethical guidelines for biomedical research involving human subjects, human cloning for reproductive purposes raises concerns about risk in relation to benefit, informed consent, and accountability. (who.int)
Human cloning for reproductive purposes is seen as having the potential to disrupt intergenerational relations and family structures, with major psychological, social and legal consequences for the individuals and communities concerned. (who.int)
Some also perceive reproductive cloning as a high-technology intervention of little relevance to the health needs of the vast majority of the world's population. (who.int)
Since then, many countries have adopted government decrees or introduced legislation to impose an explicit ban on human cloning for reproductive purposes. (who.int)

METHODS: A recombinant BALB/c-ct strain was generated and used to precisely map the ct gene. (eur.nl)

Cloning describes the processes used to create an exact genetic replica of another cell, tissue or organism. (medlineplus.gov)
The copied material, which has the same genetic makeup as the original, is referred to as a clone. (medlineplus.gov)
Cloning vectors (Cloning vehicles) are used for creating genomic libraries, preparing probes, genetic engineering, or other basic studies. (biologyexams4u.com)
Globally, there are several cloning programmes that aim to increase the size of rapidly-dwindling populations of endangered species such as African wildcats, and maintain genetic diversity through one-off clonings of individuals that haven't bred . (newscientist.com)
If this gene is defective, the person carrying the genetic defect will develop Alzheimer's. (medicalxpress.com)
The result is a reconstructed embryo, i.e. a cloned egg, which develops into a new individual with the same genetic characteristics as the gene-edited skin cell. (medicalxpress.com)
A clone is an organism that is a genetic copy of an existing one. (who.int)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
The constitutional loss of one allele of the Wilms tumor gene ( WT1 ) results in GU anomalies and forms the first of 2 genetic events in the development of a Wilms tumor. (medscape.com)
These results suggest that genetic variations in TNF, TGFB1, PTGS1 and PTGS2 genes contribute to DA susceptibility. (cdc.gov)

Should have unique restriction sites as polylinkers or multiple cloning site (MCS). (biologyexams4u.com)
Every time that you are cloning a different enzyme or gene, you might have to use a different pair of restriction sites," he said. (genomeweb.com)
As an example, Vector NTI identifies restriction sites in a plasmid, which works well for the more traditional methods of assembling DNA, such as PCR, but Hillson notes that newer tools like SLIC and the Gibson method don't use restriction enzymes or multiple cloning sites in their protocols. (genomeweb.com)

and regulation of cell cycle process. (nih.gov)
Our study demonstrates a circadian rhythm of contrast sensitivity that peaks during the daytime, and that its regulation involves interactions of D4Rs, the clock gene Npas2 , and the clock-controlled gene adenylyl cyclase 1 ( Adcy1 ) in a subset of retinal ganglion cells (RGCs). (jneurosci.org)
Several international health-related professional associations and religious bodies have issued statements calling for the careful monitoring and regulation of scientific developments in the field of cloning and human genetics. (who.int)

Potentially easier would be cloning cryogenically frozen humans, though the consensus among cloning experts is that it would be unethical and dangerous to clone a human. (newscientist.com)

Using a lentiviral vector containing either H-Ras oncogene and a hairpin targeting p53 or both tumor suppressor genes, NF-1 and p53, Dr. Friedmann-Morvinski can induce the formation of a glioma tumor in mice injected in the brain. (genengnews.com)
The induction of the dedifferentiation occurs only when two hits occur in the same cell: either H-Ras overexpression and loss of p53 or loss of tumor suppressors NF1 and p53. (genengnews.com)
A synthetic pentadecapeptide, corresponding to a region unique to INGAP, stimulated a 2.4-fold increase in [3H]thymidine incorporation into hamster duct epithelium in primary culture and a rat pancreatic duct cell line but had no effect on a hamster insulinoma tumor cell line. (jci.org)
The identification of the gene responsible for Wilms tumor did not occur until 1990, when 3 groups independently identified the WT1 gene on band 11p13. (medscape.com)
Both the GU abnormalities and the development of a Wilms tumor in patients with WAGR syndrome are related to the loss of WT1 gene function. (medscape.com)
Conjugating sugar-stabilised NPs with lectins there for is expected to multiply the potential for the early diagnosis of cancer cells and the specific release of drugs into the tumor site. (bvsalud.org)

The course presents methods to express genes, which have products that are known, unknown or not well characterized, and to analyze the gene products. (lu.se)

We know from human genetics that when the SORL1 gene is destroyed, we develop Alzheimer's. (medicalxpress.com)

The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)

Using positional cloning, Sauer and associates identified XLRS1, the gene responsible for X-linked juvenile retinoschisis. (medscape.com)

To our knowledge, this is the first report on the molecular cloning of an oxytocin/vasopressin-like receptor and its ligand from arthropods. (lu.se)
diagnostic prediction of small round blue cell tumors (SRBCT) of childhood and determining the estrogen receptor (ER) status of sporadic breast cancer. (lu.se)

Cell Death Dis, 2022 Nov 16. (nih.gov)
Cell Reports Medicine (2022). (medicalxpress.com)

Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. (sigmaaldrich.com)
Healthy mice have been cloned from cells from dead mice that had been frozen for 16 years, raising the possibility that endangered species could be cloned from old carcasses that have been tossed in freezers, rather than from living cells frozen using elaborate techniques. (newscientist.com)
Other teams have already cloned mice from previously frozen dead cells . (newscientist.com)

Any DNA molecule that has the ability to replicate inside the host to which the desired gene is integrated for cloning- include plasmids, bacteriophages, cosmids, BAC, yeast vectors, shuttle vectors etc. (biologyexams4u.com)
Cloning vectors are used for obtaining millions of copies of the cloned DNA fragments. (biologyexams4u.com)
The cloned genes in these vectors are not expected to express themselves at the transcription or translation level. (biologyexams4u.com)
Cosmids are the novel cloning vectors which possess properties of both plasmid and phage. (biologyexams4u.com)

YAC (Yeast Artificial Chromosome): A vector of hundreds to kilobases long used for cloning of DNA fragment. (biologyexams4u.com)
Cloning cell cycle regulatory genes by transcomplementation in yeast. (ox.ac.uk)

By selecting hybrid plasmids that confer an increased resistance to alkylating agents of E. coli ada - mutant cells, the B. subtilis gene dat, which encodes O 6 -methylguanine-DNA methyltransferase, was cloned. (elsevierpure.com)
This gene encodes a ubiquitously expressed member of the TALE/PBX homeobox family. (genscript.com)

In this study, the 3A and 3AB genes of O/TWN/97 were cloned using pREST, pET43 and pBlueBacHis2 plasmids. (ncl.edu.tw)

DNA fragments of Bacillus subtilis were inserted into a plasmid vector that can multiply in Escherichia coli cells, and foreign genes were expressed under the control of the lac promoter. (elsevierpure.com)
A vector used to clone DNA fragments of 100-300 kb insert size in E.coli cells. (biologyexams4u.com)
First artificial cloning vector (1977) constructed by Boliver & Rodriguez from E .coli plasmid. (biologyexams4u.com)

WHA50.37 of 1997 argues that human cloning is ethically unacceptable and contrary to human integrity and morality. (who.int)

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. (nih.gov)

Our results demonstrate that AgRenSeq is a robust protocol for rapidly discovering resistance genes from a genetically diverse panel of a wild crop relative," he says. (phys.org)
The pigs resemble Alzheimer's patients who have the SORL1 gene defects-in contrast to previous pig models for Alzheimer's, which have had one or more mutated human genes inserted in the hope of accelerating the disease," says Associate Professor Charlotte Brandt Sørensen, who has been responsible for the development of the genetically modified, cloned pigs. (medicalxpress.com)
By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)

What's more, most conservationists agree that cloning should be considered only as a last resort for species such as the northern white rhino, where all other attempts at conservation have failed, says Paul Bartels, manager of BioBankSA at the National Zoological Gardens of South Africa in Pretoria. (newscientist.com)
efforts to introduce mammoth genes into existing elephant species are more plausible. (scientificamerican.com)
The Plant Cell Culture Library (PCCL) at UMass Amherst is a globally unique collection of callus cell cultures representing more than 1,000 diverse plant species. (umass.edu)
To date, more than 20 species have been cloned , with 19 of them surviving into adulthood. (syfy.com)
In zebrafish sperm, a more alkaline pH inside the cell causes calcium ions to flow in and this influx of calcium ions triggers a unique spinning-like swimming movement that is different from the swimming of other sperm from other species. (elifesciences.org)
As the sequencing and gene annotation projects of entire genomes of many species are headed towards completion (see, e.g. [13] ), massive mapping efforts in biology are now focused on how the genes interact. (lu.se)

We have shown that if we destroy this gene in pigs, precisely the early changes occur in the animals' brain cells that we had dared to hope for. (medicalxpress.com)
As a biochemist, you will study the chemical processes that occur within the cells of living organisms. (concordia.ca)

Araçatuba virus is very similar to Cantagalo virus, showing the same signature deletion in the gene. (cdc.gov)
Meanwhile, the deletion of one copy of the PAX6 gene is responsible for aniridia. (medscape.com)
Subject A has a large deletion on chromosome 11 that removes one copy of the BDNF gene. (medscape.com)
Deletion of the PAX6 gene as part of the band 11p13 deletion in patients with AGR or WAGR syndrome results in aniridia. (medscape.com)
Deletion of the PAX6 gene, which plays a role in myelinization of the cerebral hemispheres during CNS development, may also be responsible for the mental retardation seen in the WAGR association. (medscape.com)

This facility allows for walk-in usage by users and will soon provide services including cell passage, storage, transfection, etc. (umass.edu)
The neoplastic potential of transformed cells was determined via soft agar cloning and gene transfection analysis. (cdc.gov)

The Wakayama team used a modified version of a cloning technique in which the nucleus of a mouse cell - in this case a cell from dead tissue that has been frozen and then thawed - is injected into a mouse egg that has had its nucleus removed. (newscientist.com)
In a surprise finding, the Wakayama team discovered that it was easiest to create clones from brain tissue, even though clones have never been created from living brain cells. (newscientist.com)
Wakayama speculates that freezing and thawing the tissue somehow makes it easier to "reprogram" the brain cell nucleus. (newscientist.com)
Brain tissue is also high in sugars, which can protect cells when they freeze. (newscientist.com)
But this is the first time animals have been cloned from lumps of tissue frozen without the use of chemicals that might protect the cells from damage. (newscientist.com)
Typically when data are presented, the fluor- escent intensity from the tissue is pseudo-colored red and the intensity from the reference green, and the logarithmic ratio of background corrected red and green intensities for each gene (spot) is subject to analysis (see Fig. 1 ). (lu.se)

Complementation of the mutants with the cloned gtcA in trans restored galactose and glucose on teichoic acid to wild-type levels. (asm.org)

In this study, the researchers had previously used CRISPR-Cas9-based gene editing to destroy the SORL1 gene in a skin cell taken from a minipig of the Göttingen breed. (medicalxpress.com)
Addgene: Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. (addgene.org)

For example, gene synthesis services at GenScript start at $0.55 per base pair, with a discounted price of $0.39 per base pair for genes less than 3 kilobases. (genomeweb.com)

We have found a way to scan the genome of a wild relative of a crop plant and pick out the resistance genes we need: and we can do it in record time. (phys.org)

The 14 kb human Duchenne muscular dystrophy (DMD) cDNA corresponding to a complete representation of the fetal skeletal muscle transcript has been cloned. (nih.gov)
Mus musculus RIKEN cDNA 9030624J02 gene (9030624J02Rik), transcript variant X1, mRNA. (genscript.com)

What is a cloning vector? (biologyexams4u.com)
Should have one or more marker genes for identification ad isolation of subpopulation of bacteria containing vector. (biologyexams4u.com)
The vector must be able to replicate in host cells. (biologyexams4u.com)
It is a 4362 kb long widely used cloning vector. (biologyexams4u.com)
You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart. (genscript.com)
My project was focused on deriving viral vector for application in ocular gene therapy. (lu.se)
I perform molecular cloning, cell culturing, viral vector production and microscopic imaging experiments. (lu.se)

The mitochondrial DNA of the stem cells, however, matched the donor egg's mitochondrial DNA. (news-medical.net)
Possibility to work with primary human hematopoietic stem cells. (lu.se)

Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (nih.gov)
genes and promoters. (wikipedia.org)

So that it features which are useful for molecular cloning experiments. (biologyexams4u.com)
Electrophysiological experiments then revealed that the fish version of CNGK is not activated by cyclic nucleotides, but is activated when the inside of the cell becomes more alkaline. (elifesciences.org)
In time series experiments, which for many experimental systems are confined to laboratory cell culture experiments (cell lines), each slide corresponds to a measured time point. (lu.se)
Hemagglutination experiments together with interaction studies in the presence of specific sugar show that the sugar-binding site of the lectin is distinct from the nanoparticle-binding site and cell recognition is very much intact even after binding to AgNPs. (bvsalud.org)

RESULTS: We report the absence of gross chromosomal abnormalities and the precise mapping of the ct gene to a 3-Mb region at 135 Mb (66 cM) from the centromere, closely linked to the polymorphic microsatellite marker D4Mit148. (eur.nl)

Researchers have pioneered a new method which allows them to rapidly recruit disease resistance genes from wild plants and transfer them into domestic crops. (phys.org)
The technique called AgRenSeq or speed cloning has been developed by John Innes Centre researchers alongside colleagues in the United States and Australia to speed up the fight against pathogens that threaten food crops worldwide. (phys.org)
From there researchers can use laboratory techniques to clone the genes and introduce them into elite varieties of domestic crops to protect them against pathogens and pests such as rusts, powdery mildew and Hessian fly. (phys.org)
The research published today in the journal Nature Biotechnology reveals that AgRenSeq has been successfully trialled in a wild relative of wheat-with researchers identifying and cloning four resistance genes for the devastating stem rust pathogen in the space of months. (phys.org)
What we have now is a library of disease resistance genes and we have developed an algorithm that enables researchers to quickly scan that library and find functional resistance genes," explains Dr. Sanu Arora, first author of the paper. (phys.org)
In addition to making sure researchers end up with the plasmid they want and generating large combinatorial libraries, j5 addresses "all the considerations of traditional multiple cloning site technology. (genomeweb.com)
Since the 1990s, researchers have known of three genes which-if they mutate-can directly cause Alzheimer's disease. (medicalxpress.com)
Researchers have also previously developed pig models for Alzheimer's and other diseases by means of cloning. (medicalxpress.com)
In 2009, researchers identified a potassium ion channel, called CNGK, that starts the electrical signal in the sperm cells of sea urchins. (elifesciences.org)
including some of the researchers involved in the 2009 work - now report that the CNGK channel also exists in the sperm cells of a freshwater fish, the zebrafish. (elifesciences.org)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)

The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. (origene.com)

The study also looked at the impact of paternally expressed genes: IGF2, PEG1, PEG3, DLK1, and DIO3 for transcriptomic and epigenomic alterations of the imprinted genes in fetal tissues. (genengnews.com)

One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence. (genscript.com)
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)

In addition, the morphogen retinoic acid, alone or in combination with dibutyryl cAMP, gives a 22-fold induction of PDGF alpha R gene promoter activity in human teratocarcinoma cells. (nih.gov)

Our plasmid DNA can be used for the development of various biologics, including, but not limited to, monoclonal antibodies, cell and gene therapies, and viral vaccines. (genewiz.com)

LncRNA NALT interaction with NOTCH1 promoted cell proliferation in pediatric T cell acute lymphoblastic leukemia. (nih.gov)
Downregulation of CDCA5 Can Inhibit Cell Proliferation, Migration, and Invasion, and Induce Apoptosis of Prostate Cancer Cells. (nih.gov)

The molecular interactions of genes and gene products underlie fundamental questions of biology. (lu.se)

T-cells play a crucial role in the immune response to pathogens by mediating antibody formation and clearance of infected cells, and by defining an overall response strategy. (elifesciences.org)
This approach will permit analysis of B cell clonal ontogeny, antibody diversity and lymphoma cell progression and heterogeneity. (lu.se)

Anatomy15

Organisms18

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes66

Technology, Industry, Agriculture1

Information Science4

Health Care1

Proteins8

Diseased tissues1

Protein21

Somatic cell nuc3

Embryonic stem9

Expression26

Successfully cloned2

Phenotypes4

Sequences7

Mammalian2

Reproductive12

Recombinant1

Genetic10

Restriction sites3

Regulation3

Humans1

Tumor6

Express genes1

Genetics1

Therapeutic1

Positional1

Receptor2

20222

Mice3

Vectors4

Yeast2

Encodes2

Plasmids1

Coli3

19971

Ontology1

Genetically3

Species6

Occur2

Deletion5

Transfection2

Tissue6

Complementation1

CRISPR2

Synthesis1

Genome1

Transcript2

Vector7

Stem Cells2

Promoters2

Experiments4

Marker1

Researchers11

Differentiation1

Fetal1

Accession2

Promoter1

Monoclonal antibodies1

Proliferation2

Interactions1

Antibody2