• S. R. Stürzenbaum , M. S J. Arts , and J. E. Kammenga "Molecular cloning and characterization of Cpn60 in the free-living nematode Plectus acuminatus ," Cell Stress & Chaperones 10(2), 79-85, (1 June 2005). (bioone.org)
  • basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)
  • It is ranging from cloning and molecular development to production and characterization. (lu.se)
  • Chemically synthesised oligonucleotides can also be used if the target sequence size does not exceed the limit of chemical synthesis.Isolation of insert can be done by using shotgun cloning,c-DNA clones,gene machines(artificial chemical synthesis). (bionity.com)
  • In conclusion, this first account of molecular genetic similarities and differences of Cpn60 in a neglected nematode taxon provides a valuable insight into its potential uses in gene-based ecotoxicological risk assessment exercises. (bioone.org)
  • A second gene-specific primer was designed and used in 5ʹ RACE PCR to clone the 5՛ region. (microbiologyresearch.org)
  • The products in this section are designed for molecular cloning of cDNA, gene, PCR fragments (up to 15kb) and also the construction of libraries for much larger fragments (up to 40kb) such as fosmids. (cambio.co.uk)
  • Scholars@Duke publication: Molecular cloning of the gene sequences of a major apoprotein in avian very low density lipoproteins. (duke.edu)
  • By utilizing skin secretion-derived mRNA, a cDNA library was constructed, a frenatin gene was cloned and its encoded peptides were deduced and confirmed using RP-HPLC, MALDI-TOF and MS/MS. The deduced peptides were identified as frenatin 4.1 (GFLEKLKTGAKDFASAFVNSIKGT) and a post-translationally modified peptide, frenatin 4.2 (GFLEKLKTGAKDFASAFVNSIK.NH2). (qub.ac.uk)
  • Gao, YP & Wheatly, MG 2004, ' Molecular cloning and tissue distribution of an epithelial Ca channel (ECaC) like gene from crayfish ', The FASEB Journal . (syr.edu)
  • You have successfully inserted your favorite gene into the cloning vector, and you are ready to make copies of the hybrid plasmid. (quartzy.com)
  • The encoding region of this gene was 1509 bp in length, encoding a protein containing 502 amino acids with a molecular weight of 58.66 kDa, and the isoelectric point of 5.46. (slu.se)
  • 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
  • Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications. (sigmaaldrich.com)
  • Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. (wikipedia.org)
  • Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. (wikipedia.org)
  • Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them. (wikipedia.org)
  • Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained, then the foreign DNA will be replicated along with the host cell's DNA in the transgenic organism. (wikipedia.org)
  • The recombinant protein named FoxTPI was purified (97% purity) showing a molecular mass of 27 kDa, with optimal activity at pH 8.0 and and temperature of 37 °C. The values obtained for Km and Vmax using the substrate GAP were 0.47 ± 0.1 mM, and 5331 µmol min-1 mg-1, respectively. (bvsalud.org)
  • The entire genome of human papilloma virus type 1a was cloned in Escherichia coli using the plasmid pBR322 as vector. (pasteur.fr)
  • Prior to the 1970s, the understanding of genetics and molecular biology was severely hampered by an inability to isolate and study individual genes from complex organisms. (wikipedia.org)
  • Modern cloning vectors include selectable markers (most frequently antibiotic resistance markers) that allow only cells in which the vector, but not necessarily the insert, has been transfected to grow.Additionally, the cloning vectors may contain colour selection markers which provide blue/white screening (via α-factor complementation) on X-gal medium. (bionity.com)
  • That is, these plasmids could serve as cloning vectors to carry genes. (wikipedia.org)
  • For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported. (sigmaaldrich.com)
  • In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. (wikipedia.org)
  • Cloning is frequently employed to amplify DNA fragments containing genes, but it can be used to amplify any DNA sequence such as promoters , non-coding sequences, chemically synthesised oligonucleotides and randomly fragmented DNA. (bionity.com)
  • Preparation of DNA fragments for cloning can be accomplished in a number of alternative ways. (bionity.com)
  • Subsequent cloning and sequencing of gcsap fragments obtained from PCR with genomic DNA revealed a 73 bp intron beginning at nt 728. (microbiologyresearch.org)
  • Hybridization of restriction fragments with acetate‐specific mRNA or cDNA has been used to delimit the transcribed region(s) of each clone. (psu.edu)
  • GeneArt Gibson Assembly cloning is the optimal choice for building large and demanding constructs from multiple DNA fragments. (thermofisher.com)
  • Sheared DNA can be repaired and then ligated for blunt-end cloning, genomic DNA cloning and subcloning. (cambio.co.uk)
  • Four Neurospora crassa genomic clones have been selected as hybridizing much more strongly to labeled mRNA isolated from acetate‐grown mycelium than to mRNA from sucrose‐grown mycelium. (psu.edu)
  • A cDNA clone coding for a membrane proteoglycan core protein was isolated from a neonatal rat Schwann cell cDNA library by screening with an oligonucleotide based on a conserved sequence in cDNAs coding for previously described proteoglycan core proteins. (rupress.org)
  • These findings culminated in the central dogma of molecular biology, that proteins are translated from RNA, which is transcribed from DNA. (addgene.org)
  • Here we describe cloning of this co-transporter by a method new to membrane proteins. (nature.com)
  • We have sequenced the cloned DNA and have found no homology between the Na + /glucose co-transporter and either the mammalian facilitated glucose carrier or the bacterial sugar transport proteins. (nature.com)
  • This program explores the molecular structures and functional mechanisms of proteins. (lu.se)
  • Upon graduation, you will have mastered methods for cloning and purifying proteins, as well as techniques for investigating and analyzing them. (lu.se)
  • We report the molecular cloning of DNA complementary to human Hp mRNA. (rti.org)
  • 1. Nestler, E.J. Molecular neurobiology of addiction. (lu.se)
  • The rat G-protein-coupled receptor kinase 6 (GRK6) cDNA was cloned from rat brain tissue by a combination of reverse-transcription polymerase chain reactions (RT-PCR), based on homology to the cloned human GRK6, and rapid amplification of cDNA ends (RACE-PCR). (nih.gov)
  • The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. (wikipedia.org)
  • Examples of the DNA sequences that are difficult to clone are inverted repeats, origins of replication, centromeres and telomeres. (wikipedia.org)
  • Molecular cloning is similar to polymerase chain reaction (PCR) in that it permits the replication of DNA sequence. (wikipedia.org)
  • The fundamental difference between the two methods is that molecular cloning involves replication of the DNA in a living microorganism, while PCR replicates DNA in an in vitro solution, free of living cells. (wikipedia.org)
  • WHA50.37, which states "the use of cloning for the replication of human individuals is ethically unacceptable and contrary to human integrity and morality. (who.int)
  • In the present study, six cDNAs encoding amphibian skin antimicrobial peptide precursors from the Chinese brown frog Rana chensinensis , were cloned and identified as preprobrevinin-1CEa, preprobrevinin-1CEb, preprotemporin-1CEa, preprotemporin-1CEb, preprotemporin-1CEc, and preprochensinin-1. (bioone.org)
  • Thank you for sharing this Molecular Pharmacology article. (aspetjournals.org)
  • Message Body (Your Name) thought you would be interested in this article in Molecular Pharmacology. (aspetjournals.org)
  • Intestinal carriage of prolonged spectrum β-lactamase (ESBL)-producing Escherichia coli is a frequent, rising and worrying phenomenon, however little is understood in regards to the molecular state of affairs and the evolutionary forces at play. (bioconstructor.com)
  • Electroporation is used when extremely high transformation efficiencies are required, as in very inefficient cloning strategies. (bionity.com)
  • Molecular transformation of a Neurosporaacu‐5 mutant and of an Aspergillus nidulans acuE mutant by DNA of clone 2 and clone 1, respectively, strongly suggests that clone 2 codes for acetyl‐coenzyme A synthetase and that clone 1 codes for malate synthase. (psu.edu)
  • Microbiologists, seeking to understand the molecular mechanisms through which bacteria restricted the growth of bacteriophage, isolated restriction endonucleases, enzymes that could cleave DNA molecules only when specific DNA sequences were encountered. (wikipedia.org)
  • Elevated excitability and its ac- and the molecular mechanisms of memory. (lu.se)
  • Whatever your workflow needs, our comprehensive and diverse molecular solutions are designed for high performance, time savings, and reliability for your PCR and cloning success. (thermofisher.com)
  • A cDNA clone encoding a protein consisting of 189 amino acid residues including a sodefrin sequence was isolated from a Cynops pyrrhogaster abdominal gland cDNA library. (wiley.com)
  • Likewise, a cDNA clone encoding a molecule comparable to the sodefrin precursor was obtained from a Cynops ensicauda abdominal gland cDNA library. (wiley.com)
  • The open reading frame encodes a polypeptide of 365 amino acids with a molecular mass of 39,514 Da. (elsevierpure.com)
  • We have developed and manufactured a portfolio of more than 300 reagents and kits, many of them proprietary, for molecular biology, cell analysis and nucleic acid and protein separation and purification. (gcbiotech.com)
  • Cloning is utilized in a wide array of biological experiments and technological applications such as large scale protein production. (bionity.com)
  • In practice, however, a number of other features are desired and a variety of specialised cloning vectors exist that allow protein expression, tagging, single stranded RNA and DNA production and a host of other manipulations that are useful in downstream applications. (bionity.com)
  • We obtained a clone of 2817 bp with an open reading frame of 1731 bp encoding for a protein of 576 amino acids that is 96.7% identical and 97.9% similar to its human counterpart. (nih.gov)
  • The GPCR97 clone was used to probe a human thalamus library, which resulted in the isolation of a full-length clone encoding a putative G protein-coupled receptor. (aspetjournals.org)
  • This clone encoded a precursor protein of 192 amino acid residues, including a sodefrin-like peptide sequence with substitutions of two amino acid residues. (wiley.com)
  • IgG fraction purified by immobilized Protein A. Clone 8H9D4, isotype IgG1. (mol-innov.com)
  • In the classical restriction and ligation cloning protocols, cloning of any DNA fragment essentially involves four steps: fragmentation, ligation, transfection, and screening/selection. (bionity.com)
  • Viral DNA isolated from a single wart was partially methylated at only one out of the four HpaII sites, d(C-C-G-G). Recognition sites for Bg/I, Bg/II, PstI and PvuII restriction endonucleases were located on the cloned genome. (pasteur.fr)
  • [Molecular cloning] refers to the procedure of isolating a defined DNA sequence and obtaining multiple copies of it in vivo . (bionity.com)
  • We aimed to identify isolates of this clone using Vitek 2 SXT resistance as a proxy and to compare its epidemiology with those of other circulating S. aureus strains. (edu.au)
  • cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)
  • We are pleased to announce Molecular Innovations is now a part of Innovative Research, Inc. We pledge to continue providing the same high quality research reagents, technical support, and customer service you have come to expect for more than twenty-five years. (mol-innov.com)
  • Molecular Innovations started from humble roots to become a leading commercial source of hemostasis and thrombolysis research tools. (mol-innov.com)
  • Infectious Molecular Clone of a Recently Transmitted Pediatric Human I" by Ricky D. Grisson, Agnes-Laurence Chenine et al. (unl.edu)
  • 2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
  • WHA50.37 of 1997 argues that human cloning is ethically unacceptable and contrary to human integrity and morality. (who.int)
  • General Assembly the adoption of a declaration on human cloning by which Member States were called upon to prohibit all forms of human cloning inasmuch as they are incompatible with human dignity and the protection of human life. (who.int)
  • 3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
  • 7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
  • 3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)
  • 5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
  • Cloning of a human parvovirus by molecular screening of respiratory tract samples. (medscape.com)
  • Before actual cloning experiments are performed in the lab, most cloning experiments are planned in a computer, using specialized software. (wikipedia.org)
  • Molecular cloning methods are central to many contemporary areas of modern biology and medicine. (wikipedia.org)
  • This changed dramatically with the advent of molecular cloning methods. (wikipedia.org)
  • You will also find learning and support resources for molecular biology techniques and methods, available to you at your fingertips. (thermofisher.com)
  • This ST5-MRSA-SCCmec IVo clone is reported as resistant to trimethoprim-sulfamethoxazole by Vitek 2 but susceptible by phenotypic methods. (edu.au)
  • Molecular Therapy - Methods and Clinical Development. (lu.se)
  • With Applied Biosystems and Invitrogen products for PCR and cloning, there is no wondering whether they will propel your research forward. (thermofisher.com)
  • Are you looking for Thermo Scientific molecular biology products? (thermofisher.com)
  • The data collected from the resultant spectra were used to assess differences among clones and among ramets of a single clone and to determine the mechanism governing the observed differences. (who.int)
  • 1. Cloning is an umbrella term traditionally used to describe different processes for duplicating biological material. (who.int)
  • A clone is an organism that is a genetic copy of an existing one. (who.int)
  • Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
  • Our molecular reagents are used by biologists and other research scientists to perform test-assays and research in many fields from medical, biotechnology and marine biology to food and agriculture technology as well as forensic and environmental sciences, where life scientists have come to depend upon the outstanding quality and reliability of our reagents. (gcbiotech.com)