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  • gene
  • The contents of the two in- troductory chapters are intended to provide the reader with a brief background of the approaches to gene cloning and the struc- ture and expression of hormone-encoding genes. (springer.com)
  • involving the use of restriction enzymes DNA sequencing (where the sequence of nucleotides is known and the gene can be synthesized in the laboratory) Nucleic acid hybridization (used to locate the target gene on its chromosome) DNA cloning (the gene is inserted into the genome of a foreign organism (host) and is replicated as the cells in the host divide). (scribd.com)
  • The objective of Genetic engineering in this case is to clone the insulin gene from the human genome. (scribd.com)
  • I am trying to clone a promoter controlling the expression of a reporter gene. (protocol-online.org)
  • Struggling to clone your gene of interest or wasting precious time troubleshooting molecular cloning procedures? (genscript.com)
  • genomic
  • I would suspect that you will see a lot of large molecular weight material that would correspond to the genomic DNA, again it would depend on what kind of protocol you were using to isolate the DNA in the first place, but it might give you some indication of how pure it is. (protocol-online.org)
  • I am trying to shotgun clone short pieces of DNA for sequencing purposes and the protocol is to digest the DNA (human genomic) with MseI then ligate on linkers (also digested with MseI). (protocol-online.org)
  • molecules
  • The use of the word cloning refers to the fact that the method involves the replication of a single DNA molecule starting from a single living cell to generate a large population of cells containing identical DNA molecules . (primidi.com)
  • Strictly speaking, recombinant DNA refers to DNA molecules, while molecular cloning refers to the experimental methods used to assemble them. (primidi.com)
  • identification
  • After more than half a century of research and development, the applications of REases have evolved from the cloning of exogenous DNA and genome mapping to more sophisticated applications, such as the identification and mapping of epigenetic modifications and the high-throughput assembly of combinatorial libraries. (neb.com)
  • organism
  • In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. (primidi.com)
  • large
  • Cloning not only allowed the production of large quantities of highly purified enzymes, but also made the engineering of REases possible. (neb.com)