• Arabidopsis thaliana provides an excellent experimental plant system for molecular genetics because of its remarkably small genome size, near absence of dispersed middle repetitive DNA, and short life cycle. (caltech.edu)
  • Barley and maize are two important crops in the world and also excellent experimental organisms particularly in the area of molecular genetics. (edu.au)
  • Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892. (asm.org)
  • Extremely useful in biotechnology, bioengineering, and molecular genetics, MCSs let a molecular biologist insert a piece of DNA or several pieces of DNA into the region of the MCS. (wikipedia.org)
  • A doctor who separately is pursuing human cloning has reported in an Internet journal preliminary data on an early-stage cloned human embryo, but with no chromosome information. (wired.com)
  • Indeed, inside cloned monkey cells, the Pittsburgh researchers discovered deformed spindles and chaotic chromosome numbers. (wired.com)
  • involving the use of restriction enzymes DNA sequencing (where the sequence of nucleotides is known and the gene can be synthesized in the laboratory) Nucleic acid hybridization (used to locate the target gene on its chromosome) DNA cloning (the gene is inserted into the genome of a foreign organism (host) and is replicated as the cells in the host divide). (scribd.com)
  • Our molecular data reveal that the suppressor of forked locus, which on cytogenetic grounds is the most proximal ordinary gene on the X chromosome, is very close to the junction of the polytenized and non-polytenized region of the X chromosome. (genetics.org)
  • High accuracy robotics combined with gentle fluidics-based systems establish viable clones with much higher efficiency. (moleculardevices.com)
  • In vivo methyl labeling experiments with L-[methyl-3H]methionine showed that this knockout mutant lacked an MCP with a molecular weight of approximately 68,000. (asm.org)
  • From this starting point a series of YAC, cosmid and phage clones were assembled into a genomic walk covering over 400 kb. (genetics.org)
  • Papilloma virus DNA from a laryngeal papilloma was cloned in phage lambda L 47 and characterized after cleavage with different restriction enzymes. (asm.org)
  • The insert from this clone, clone 17, was then used to screen an oligo-dT-primed Z210R.1 cDNA library made in λgt10, from which two other clones (2-1 and 1A) were isolated. (rupress.org)
  • Although there is no stretch of polyA at the 3′ end of our clones, it is likely that the AATAAA sequence at nucleotides 1111-1116 represents a polyadenylation signal, since the clone containing it was isolated from an oligo-dT-primed cDNA library. (rupress.org)
  • WASHINGTON - Cloning humans, or any other primates, may be impossible with today's techniques because of a fundamental molecular obstacle, say scientists trying to understand why attempts to clone monkeys have failed. (wired.com)
  • After more than half a century of research and development, the applications of REases have evolved from the cloning of exogenous DNA and genome mapping to more sophisticated applications, such as the identification and mapping of epigenetic modifications and the high-throughput assembly of combinatorial libraries. (neb.com)
  • RESULTS: The dioscorin gene families of D. japonica ( Dj-dioA1-Dj-dioA4, Dj-dioB1 and Dj-dioB2 ) and D. pseudojaponica ( Dp-dioA1-Dp-dioA5 and Dp-dioB1 ) were cloned from cDNA libraries of yam tubers. (wiley.com)