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A ribophorin I cDNA clone containing a 0.6-kb insert was isolated from a rat liver lambda gtll cDNA library by immunoscreening with specific antibodies. (rupress.org)
This cDNA was used to isolate a clone (2.3 kb) from a rat brain lambda gtll cDNA library that contains the entire ribophorin I coding sequence. (rupress.org)
This confirmed the identity of the cDNA clone and established that ribophorin I contains 583 amino acids and is synthesized with a cleavable amino terminal insertion signal of 22 residues. (rupress.org)
Hybridization measurements using the cloned cDNA as a probe showed that ribophorin I mRNA levels increase fourfold 15 h after partial hepatectomy, in confirmation of measurements made by in vitro translation of liver mRNA. (rupress.org)
The cDNA VH-D-JH gene sequence was joined with that of the C alpha gene in the B cells of the three IgA CLLs, and with that of the C gamma gene in the IgG CLL B cells. (omicsdi.org)
In the IgA/IgG-coexpressing CLL B cells, identical VH-D-JH cDNA sequences were spliced to either C alpha or C gamma genes. (omicsdi.org)
The isolation, characterization, and expression of a full-length cDNA encoding the human T cell glycoprotein CD6 is described. (embl.de)
The protocol involves the transformation of pools of resistant cells by cDNA expression libraries, followed by the selection for rare virus-sensitive cells, using multiple rounds of selection after infection by marked viral vector genomes. (biomedcentral.com)
We here report the isolation of two cDNA constructs that each restore virus sensitivity to the R4-7 mutant cell line. (biomedcentral.com)

A skewed preference for BV7 genes and sequential "G" amino acids motif is observed in complementarity-determining region-3 of T cell receptor-β. (nature.com)
Increased binding scores are observed in T-cell clones with complementarity-determining region-3 of T cell receptor-β with a "(G)GG" motif. (nature.com)
An individual T-cell has a specific complementarity-determining region 3 (CDR3) of T cell receptor (TCR)-α and -β, which is a result of the recombination of somatic TCR V-(D)-J genes and junction diversity. (nature.com)
Sequencing of the T-cell receptors of the OspA reactive clones showed significant skewing of the T-cell receptor repertoire. (ox.ac.uk)
In addition, we show that both mPR beta and PGMRC1, two members of distinct membrane-bound progestin receptor classes, exhibit highly similar ovarian expression profiles during the reproductive cycle with maximum levels during vitellogenesis and a down-expression during late vitellogenesis. (biomedcentral.com)
Thus, in amphibians, it has been assumed for a long time, that the actinomycin D-insensitive progesterone-induced oocyte maturation [ 4 ] was mediated by a membrane steroid receptor rather than a nuclear receptor. (biomedcentral.com)
The recent cloning and sequencing of a gene coding for a membranous progesterone receptor [ 5 ] in African clawed frog ( Xenopus laevis ) brought evidence for the existence of both intracellular and membrane progestin receptors [ 6 ]. (biomedcentral.com)
Furthermore, membrane receptor capacity of the spotted seatrout ovary has been shown to be correlated with oocyte responsiveness to 17,20β,21P for maturation, assessed by the occurrence of germinal vesicles breakdown (GVBD) [ 16 ]. (biomedcentral.com)
These B cells appear to be blocked at the antigen receptor-expressing stage of B cell differentiation and are thought not to undergo an isotype class switch to IgG or IgA production. (omicsdi.org)
Cloning of a novel bacteria-binding receptor structurally related to scavenger receptors and expressed in a subset of macrophages. (embl.de)
The results suggest that the novel protein is a macrophage-specific membrane receptor with a role in host defense, as it shows postnatal expression in macrophages, which are considered responsible for the binding of bacterial antigens and phagocytosis. (embl.de)
Studies utilizing cloned native and mutant forms of the scavenger receptor have provided insights into the structural basis for their function. (embl.de)
The extracellular domain of CD6 is significantly related to the extracellular domain of the human and mouse T cell antigen CD5, the cysteine-rich domain of the bovine and mouse type I macrophage scavenger receptor, the extracellular domain of the sea urchin spermatozoa protein that crosslinks the egg peptide speract, the mammalian complement factor 1, and the human lung tumor antigen L3. (embl.de)

Genetic selections in mammalian cell lines have recently been developed for the isolation of mutant cells that are refractory to infection by retroviruses. (biomedcentral.com)

Tandem repeats of SRCR domains are common in the membrane bound proteins. (embl.de)
Macrophage scavenger receptors are integral membrane proteins whose ability to bind and degrade modified LDL has implicated them in the process of atherosclerotic foam cell formation. (embl.de)
The lymphocyte glycoprotein CD6 contains a repeated domain structure characteristic of a new family of cell surface and secreted proteins. (embl.de)

In lower vertebrates, steroid-induced oocyte maturation is considered to involve membrane-bound progestin receptors. (biomedcentral.com)
Two totally distinct classes of putative membrane-bound progestin receptors have been reported in vertebrates. (biomedcentral.com)

Analysis of the amino acid sequence of ribophorin I suggested that the polypeptide has a simple transmembrane disposition with a rather hydrophilic carboxy terminal segment of 150 amino acids exposed on the cytoplasmic face of the membrane, and a luminal domain of 414 amino acids containing three potential N-glycosylation sites. (rupress.org)
The cytoplasmic domain of CD6 contains two serine residues, one or both of which are substrates for phosphorylation during T cell activation. (embl.de)

A common theme demonstrated by these analyses is that distinct bacterial clones are responsible for disease outbreaks and increases in infection frequency. (cdc.gov)
One of the primary themes emerging from this research is that distinct bacterial clones have been responsible for several infection outbreaks ( Table ). (cdc.gov)
Moreover, the distinct clones are frequently characterized by unique combinations of virulence genes or alleles of virulence genes. (cdc.gov)
Complete sequencing of both alpha and beta chains of a subset of clones showed that at least two distinct T-cell clones were expanded in vivo. (ox.ac.uk)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

Such information is mediated immunity, secreting the cytokine crucial to determine the optimal approach interferon-gamma (IFN) that inhibits Th2 to improve the immune response of DN cell differentiation. (who.int)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)

It is becoming increasingly apparent that mammalian cells harbor numerous genes that induce intracellular blocks to retrovirus infection [ 1 , 2 ]. (biomedcentral.com)

The identification of CMV-CTL clones is one method used to assess cellular immunity against CMV. (nature.com)

We previously established a direct single-cell analysis to simultaneously identify and quantify in vivo CMV-CTL clones after allo-HCT 9 , 10 . (nature.com)
Characterization of in vivo expanded OspA-specific human T-cell clones. (ox.ac.uk)
Examples of in vivo isotype class switching in IgM+ chronic lymphocytic leukemia B cells. (omicsdi.org)
To test the hypothesis that in vivo isotype class switching occurs in IgM+ B-type CLL cells, we analyzed the PBMC of 19 CLL patients for the presence of transcripts encoding the rearranged CLL V(H)DJ(H) associated with either gamma or alpha H chains. (omicsdi.org)
1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)

On the other hand, bulk RNA-sequence analyses reveal that gene expression profiles among patients are different according to the cytomegalovirus reactivation pattern, and are associated with cytokine production or cell division. (nature.com)

We are among the first to define the characteristics of TCR usage of T cells isolated from an inflamed immune compartment in an individual with an autoimmune disease potentially triggered by a microbial antigen. (ox.ac.uk)

A panel of CD4 T-cell clones was isolated from synovial fluid by single cell flow cytometry from a patient with treatment-resistant Lyme arthritis using a DRB1*0401 major histocompatibility complex (MHC) class II tetramer covalently loaded with outer surface protein A (OspA) peptide164-175, an immunodominant epitope of Borrelia burgdorferi. (ox.ac.uk)
These isotype-switched CLL B cells expressed surface HLA-DR, CD19, CD23, and CD5, and displayed no alterations of the bcl-1 and bcl-2 oncogenes and the p53 tumor-suppressor gene. (omicsdi.org)
The cellular data indicate that B cells with lesser amounts of surface membrane IgD and higher IgM/IgD ratios are more likely to undergo this switching process. (omicsdi.org)
Collectively, these data suggest that not all members of a B-CLL clone are frozen at the surface membrane Ig-expressing stage of B cell maturation, and that some members can switch to the production of non-IgM isotypes. (omicsdi.org)

SP6 RNA transcripts of the insert in this clone directed the in vitro synthesis of a polypeptide of the expected size that was immunoprecipitated with anti-ribophorin I antibodies. (rupress.org)
Chronic lymphocytic leukemia (CLL) usually involves the expansion of a clone of CD5+ B cells synthesizing IgM antibodies. (omicsdi.org)
COS cells transfected with the CD6 clone express a 90-kD protein that reacts with all available anti-CD6 monoclonal antibodies. (embl.de)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

Analysis of heterologous expression systems of rOAT1 in oocytes and culture cells revealed that rOAT1 has the ability to transport anionic drugs, such as β-lactam antibiotics, NSAIDs, methotrexate, and antiviral drugs, as well as various endogenous organic anions and exogenous substances. (aspetjournals.org)

We have been involved in the development of screens and selections for virus resistance genes, and have isolated mutant cell lines after chemical mutagenesis that are profoundly resistant to retrovirus infection. (biomedcentral.com)
To probe the nature of the blocks in these mutant cell lines, we have sought to identify and characterize suppressor genes that override the restriction exhibited by these cells. (biomedcentral.com)
The R4-7 mutant cell line is approximately 100-fold resistant to transduction by MuLV-based vectors as compared to wild-type Rat2 cells [ 6 ]. (biomedcentral.com)

Single-cell RNA-sequence analyses demonstrate the homogenous distribution of the gene expression profile in individual cytomegalovirus-specific cytotoxic T-cells within each recipient. (nature.com)

Here we show the features of repertoire diversity and the gene expression profile of HLA-A24 cytomegalovirus-specific cytotoxic T-cells in actual recipients according to the cytomegalovirus reactivation pattern. (nature.com)
However, little information is available on the gene expression profile (GEP) of CMV-CTL clones according to CMV reactivation patterns (and donor CMV serostatus) after allo-HCT. (nature.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

RNA blot hybridization analysis indicates that CD6 transcripts are predominantly restricted to cells in the T lineage. (embl.de)

In the present study, we report the characterization of the full coding sequence of rainbow trout PGMRC1 and mPR beta cDNAs, their tissue distribution, their ovarian expression profiles during oogenesis, their hormonal regulation in the full grown ovary and the in situ localization of PGMRC1 mRNA in the ovary. (biomedcentral.com)
The predicted CD6 sequence is 468 amino acids long, with the typical features of a type I integral membrane protein. (embl.de)

In all five CLLs, the pattern of C mu DNA probe hybridization to the digested genomic DNAs was consistent with deletion of the C mu exon from the rearranged Ig gene locus, suggesting that these CLL B cells had undergone DNA switch recombination. (omicsdi.org)

The hOAT3 protein was shown to be localized in the basolateral membrane of renal proximal tubules and the hOAT3 gene was determined to be located on the human chromosome 11q12-q13.3 by fluorescent in situ hybridization analysis. (aspetjournals.org)
Diabetic nephropathy (DN) is a leading selectin), and soluble thrombomodulin--are cause of chronic renal failure and is a grow- providing further evidence of the relation- ing concern given the increasing incidence ship between endothelial cell activation and of type 2 diabetes. (who.int)
found evidence and they did not require insulin therapy of endothelial cell injury in renal failure for glucose control. (who.int)

A novel murine plasma membrane protein has been identified in subpopulations of macrophages. (embl.de)
Transfected COS cells synthesized a native trimeric plasma membrane protein that bound labeled bacteria and acetylated LDL, but not yeast or Ficoll. (embl.de)

Cytomegalovirus reactivation is still a critical concern following allogeneic hematopoietic cell transplantation, and cellular immune reconstitution of cytomegalovirus-specific cytotoxic T-cells is necessary for the long-term control of cytomegalovirus reactivation after allogeneic hematopoietic cell transplantation. (nature.com)
These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
However, cellular immune reconstitution of CMV-specific cytotoxic T-cells (CMV-CTL) is generally considered to be necessary for the long-term control of CMV reactivation after allo-HCT. (nature.com)
A better and deeper understanding of immune reconstitution following allo-HCT is required for further clinical application of these adoptive T-cell therapies. (nature.com)

B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

In many instances, these CLL-related gamma and alpha transcripts are much more plentiful than those of normal B cells that produce the same isotype. (omicsdi.org)

Th2 cells produce cy- patients which might reduce the morbidity tokine interleukins IL-4 and IL-10, which and mortality due to infection. (who.int)

The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)

Many of these clones are characterized by unique combinations of virulence genes or alleles of virulence genes. (cdc.gov)
The immunoglobulin (Ig) variable region (V) genes expressed by IgM chronic lymphocytic leukemia (CLL) B cells display little or no somatic mutations. (omicsdi.org)
Lack of intraclonal diversification in Ig heavy and light chain V region genes expressed by CD5+IgM+ chronic lymphocytic leukemia B cells: a multiple time point analysis. (omicsdi.org)
The suppressor genes were then recovered from these virus sensitive cells, and their ability to restore virus susceptibility was confirmed by reintroduction of these cDNAs into the resistant line. (biomedcentral.com)
To identify such genes, we have developed methodologies that allow for the selection of rare virus-sensitive clones arising after transfer of gene libraries into populations of virus-resistant parents. (biomedcentral.com)

In addition, T-cell clones were tested functionally for their reactivity to the wild-type peptide as well as to altered peptide ligands (APLs) and peptide libraries based on the OspA epitope in order to determine the TCR contact residues and the stringency in T cell recognition. (ox.ac.uk)

Because substantial interclonal variance exists in relative virulence, molecular population genetic studies have led to the concept that the unit of bacterial pathogenicity is the clone or cell line. (cdc.gov)
Two such lines isolated from a parental fibroblast cell line, Rat2 cells, have been characterized in some detail [ 6 ]. (biomedcentral.com)

Of the 101 T-cell clones sequenced, 81 possessed TCR beta chains that were present in at least one other clone isolated. (ox.ac.uk)

each group of cell lines was established. (lu.se)

The viral DNA was apparently trapped in the cytoplasm in a form that was not readily extracted by conditions that allowed DNA recovery from wild-type infected cells. (biomedcentral.com)

These findings suggest that IgA- and/or IgG-expressing CLLs represent, in their VH gene structure, transformants of B cells at different stages of ontogeny. (omicsdi.org)
They also suggest that Ag may play a role in the clonal selection of some of these isotype-switched leukemic cells, but bcl-1 and bcl-2 oncogene rearrangements and p53 tumor suppressor gene mutation are not associated with the pathogenesis of isotype-switched CLLs. (omicsdi.org)

The molecular data indicate that approximately 50% of B-CLL patients have amplifications of IgM+ B cells that undergo an isotype class switch. (omicsdi.org)

Anatomy46

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CDNA9

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Isolation1

Proteins3

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Antigen1

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