Clones sequenced expressed sequence tags. Medical search. Frequent questions

Aspergillus flavus expressed sequenced tags (ESTs) provides a rapid and effective method for identification of the genes involved in aflatoxin formation and promises effective control strategy being developed through biotechnology. (usda.gov)
Genes known to be highly expressed in β-cells were represented at a high frequency, namely insulin (15 of 80 clones), islet amyloid polypeptide (8 of 80 clones), proinsulin convertase 1 (6 of 80 clones), and neuropeptide Y (2 of 80 clones). (diabetesjournals.org)
Moreover, genes known to be important in the innate and adaptive immune responses of terrestrial mammals and in responses to stress and contaminant exposure have been targeted for cloning using PCR-based techniques. (vin.com)
A total of 62 dolphin genes of known stress or immune function have been cloned by targeted PCR (including immunoglobulins, T-cell receptors, cytokines, Toll-like receptors, stress proteins and toxin response proteins). (vin.com)
The 2200 unigenes from the EST collection and the 62 immune-function targeted genes, together with other genes randomly selected without sequencing from the cDNA libraries, have been amplified and used to construct a cDNA microarray representing 3700 dolphin genes in duplicate for a total of 7400. (vin.com)
Genetic and molecular analyses have led to the identification, cloning and characterization of a few genes that contribute to parasitic fitness in the pathogens. (sisef.it)
Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
These analyses have also helped identify genes that were differentially expressed in DED fungi grown under defined experimental conditions. (sisef.it)
A total of 389 unigenes had significant homologies with known protein sequences at the NCBI database and the remaining 199 unigenes were designated unidentified genes. (korea.ac.kr)
Of the 233 randomly selected clones, 25 were identified as differentially expressed genes between 5MT resistant and 5MT sensitive wild type plants. (korea.ac.kr)
Sequences of DNA in the genes that are located between the EXONS. (lookformedical.com)
METHODS: To identify expressed TNFSF genes, we searched a computer database of ~50,000 rainbow trout expressed sequence tags generated at the National Center for Cool and Cold Water Aquaculture. (usda.gov)
Three introns were identified in the rainbow trout, Atlantic salmon and zebrafish genomic sequences, and the intron locations correspond closely to those in the mammalian TNFSF 13b genes. (usda.gov)
The physical map provides a framework for assembly of genome sequence and information for localization of genes that are difficult to find through positional cloning. (frontiersin.org)
Cloning and characterization of genes specifically expressed during infection stages in the rice blast fungus. (zju.edu.cn)
cDNA subtractive cloning of genes expressed during early stage of appressorium formation by Magnaporthe grisea . (zju.edu.cn)
Analysis of genes expressed during rice- Magnaporthe grisea interactions. (zju.edu.cn)
4] "In silico identification and expression of SLC30 family genes: an expressed sequence tag data mining strategy for the characterization of zinc transporters' tissue expression. (tcdb.org)
Genes preferentially expressed in bovine oocytes revealed by subtractive and suppressive hybridization. (appliedbioinfo.com)
The subtracted library was characterized by sequencing 185 random clone inserts, representing 146 nonredundant genes. (appliedbioinfo.com)
B-cell translocation gene 4, cullin 1, MCF.2 transforming sequence, a locus similar to snail soma ferritin, and three unidentified genes were, indeed, preferentially expressed in the oocyte, even though most were also highly expressed in testis. (appliedbioinfo.com)
To systematically discover ovarian fertility genes en masse, we employed a mouse model (Foxo3) in which follicles are assembled normally but then undergo synchronous activation. (appliedbioinfo.com)
These sequences will be subjected to further researches on hardiness genes and their molecular mechanisms. (geneticsmr.com)
In yeast a single complex of three proteins termed HAP2 HAP3 and HAP5 binds to this sequence and in mammals the three components of the equivalent complex (called variously NF-Y CBF or CP1) are also represented by single genes. (camregbase.org)

We identified zebrafish and Fugu TNFSF 13b homologues by genomic DNA database searches and determined that the predicted proteins share 68% and 76% sequence identity with trout TNFSF 13b. (usda.gov)
Bacterial artificial chromosome clones have been isolated from a trout library to further facilitate genomic characterization. (usda.gov)
However, understanding of quantitative traits and use of genomic tools for breeding is partly restricted by the large expected genome size of 3,947 to 4,397 Mbp/1C ( Arumuganathan and Earle, 1991 ) and the occurrence of highly repetitive sequences in the pea genome. (frontiersin.org)
Despite the advent of next-generation sequencing technologies, the above mentioned attributes of the wheat genome have rendered the assembly of genomic sequences extremely difficult. (biomedcentral.com)
After Blast analysis within GenBank, 64% could be identified, 21% were homologous to unannotated expressed sequence tag (EST) or genomic sequences, and 15% were novel. (appliedbioinfo.com)
Additional homologs (two of AtHAP3 and one of AtHAP5) have been identified from available Arabidopsis genomic sequences. (camregbase.org)
Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)

In order to reduce and eliminate aflatoxin contamination of food and feed, a project to generate Aspergilllus flavus Expressed Sequence Tags (ESTs) have been initiated. (usda.gov)
From the two cDNA-libraries 24,000 ESTs were collected and a total of 2200 unigenes have been sequenced and annotated ( www.marinegenomics.org ). (vin.com)
A total of 35 microsatellites were identified from the ESTs, with 18 having sufficient flanking sequences for primer design. (nih.gov)
A total of 5,542 ESTs were generated representing 4,688 unique sequences. (nih.gov)
Expressed sequenced tags (ESTs) of 1 019 randomly selected clones were analyzed and then assembled 588 unigens. (korea.ac.kr)
Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in GenBank. (zju.edu.cn)
Sequences of loblolly pine primer pairs from xylem ESTs and seedling ESTs suitable as anchor loci for comparative mapping. (ucdavis.edu)
The leaves were harvested at various times after cold stress for total RNA extraction, which was used to generate expressed sequence tags (ESTs). (geneticsmr.com)
After randomly choosing, cloning and sequencing, 227 ESTs with high quality were obtained and submitted to GenBank database. (geneticsmr.com)
Using BLASTX, 79.7% of the ESTs shared homology with known functional DNA sequences and 20.3% shared significant homology with unknown proteins. (geneticsmr.com)

The corresponding full-length cDNAs were cloned and defined as HDAC4, HDAC5, and HDAC6. (psu.edu)
Three independent Arabidopsis HAP subunit 2 (AtHAP2) cDNAs were cloned by functional complementation of a yeast hap2 mutant and two independent forms each of AtHAP3 and AtHAP5 cDNAs were detected in the expressed sequence tag database. (camregbase.org)

There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. (lookformedical.com)
Transposable elements (TEs) and satellite DNAs, two major categories of repetitive sequences, are expected to accumulate in non-recombining genome regions, including sex-linked regions, and contribute to sex chromosome evolution. (bvsalud.org)
To address the challenges of accurate assembly of the pea genome (∼4.2 GB of which approximately 85% is repetitive sequences), we have adopted the WGP technology for assembly of a pea BAC library. (frontiersin.org)
In the current study, we have undertaken construction of a sequence-based physical map of pea to address the challenge in the assembly of these repetitive sequences and overcome the shortcomings of traditional restriction digestion based physical maps. (frontiersin.org)

The amino acid sequence of Hda1p was used to search the Gen-Bank/expressed sequence tag databases to identify partial sequences from three putative class II human HDAC proteins. (psu.edu)

1] "Complete sequencing and characterization of 21,243 full-length human cDNAs. (tcdb.org)

It is also called nucleotide sequence. (lookformedical.com)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. (lookformedical.com)

The construction of BAC-based physical maps of individual chromosomes reduces the complexity of this allohexaploid genome, enables elucidation of gene space and evolutionary relationships, provides tools for map-based cloning, and serves as a framework for reference sequencing efforts. (biomedcentral.com)
In the absence of a finished quality genome sequence, insights into wheat genome structure and function have been accumulating through survey sequencing of individual chromosomes or chromosome-specific Bacterial Artificial Chromosome (BAC) libraries. (biomedcentral.com)
An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family. (le.ac.uk)
Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. (le.ac.uk)

Of these, 17 were identical or homologous to known mammalian cDNAs or expressed sequence tags. (diabetesjournals.org)

Cluster analysis indicated the presence of 635 contigs and 4,053 singletons, generating a total of 4,688 unique sequences. (nih.gov)
Whole genome profiling (WGP) is a sequence-based physical mapping technology and uses sequence tags generated by next generation sequencing for construction of bacterial artificial chromosome (BAC) contigs of complex genomes. (frontiersin.org)
These WGP tags were used to assemble 220,013 BACs into contigs. (frontiersin.org)
Assembly of the BAC clones using the modified Fingerprinted Contigs (FPC) program has resulted in 13,040 contigs, consisting of 213,719 BACs, and 6,294 singleton BACs. (frontiersin.org)
WGP technology is based on generation of short sequence tags from terminal ends of restriction fragments of individual BAC clones, followed by assembly of BAC clones into contigs based on shared regions containing identical sequence tags. (frontiersin.org)

BACKGROUND: Long terminal repeat (LTR)-retrotransposons (LTR-RTs) are ubiquitous and make up the majority of nearly all sequenced plant genomes, whereas their pivotal roles in genome evolution, gene expression regulation as well as their epigenetic regulation are still not well understood, especially in a large number of closely related species. (bvsalud.org)

A total of 1,960 nonredundant sequences (NRSs) were generated for N. oculata clone CS-179. (academicconcepts.net)

The SNAP-tag ® is a small protein based on mammalian O 6 -alkylguanine-DNA-alkyltransferase (AGT). (neb.com)
The transporter was identified by probing a mammalian-expressed sequence tag database with 2 fungal mitochondrial ornithine carrier protein sequences. (medscape.com)

Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
Transgenic frogs that express the enzyme in the germline can be bred with animals harboring a transposon substrate to generate double transgenic lines where remobilization will occur in the germline in subsequent generations. (biomedcentral.com)
16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)

The deduced amino acid sequence of hOAT3 showed 36 to 51% identity to those of other members of the OAT family. (aspetjournals.org)

Systematic sequencing of cDNA clones derived from xylem and leaf tissue will produce a new generation of research tools for conifer biology. (le.ac.uk)

A polymerase chain reaction-based subtractive hybridization procedure was applied to cDNAs prepared from mouse insulinoma (βTC3) and glucagonoma (αTC2) cell lines to construct a library of cDNAs that are highly expressed in pancreatic β-cells. (diabetesjournals.org)

The tagged DNA binds to its complementary DNA segment and can be detected by measuring the amount and type of fluorescence. (msdmanuals.com)

SNAP-tag protein (MW: 19,694) was expressed and purified according to the instructions in the IMPACT™ kit manual ( NEB #E6901 ). (neb.com)
The purified SNAP-tag protein was dialyzed into 1X phosphate buffered saline (PBS) solution containing 1 mM DTT at 1 mg/ml (50 μM) and stored at -80°C. (neb.com)
The SNAP-tag Purified Protein may be stored at -20°C for short term use, but should be kept at -80°C for long term storage. (neb.com)
SNAP-tag Purified Protein is supplied at a concentration of 50 μM and is supplied in 1X PBS with 1mM DTT. (neb.com)
These motifs contain 2 hydrophobic alpha-helical segments connected by an extensive hydrophilic sequence, resulting in 6 transmembrane portions of the protein. (medscape.com)

We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

Almost 2,000 expressed sequence tag (EST) cDNA clones were sequenced to analyze gene expression in control and Cd-treated leaf trichomes. (uni-bayreuth.de)

An Atlantic salmon gene has been cloned that is identical to trout TNFSF 13b. (usda.gov)

In this study, we thoroughly examined the repetitive components of male and female H. scandens using next-generation sequencing data followed by bioinformatics analysis and florescence in situ hybridization (FISH). (bvsalud.org)
WGP is designed based on the use of sequence tags generated by next generation sequencing (NGS) and is a powerful alternative to traditional DNA fingerprinting based physical mapping technologies, and also simultaneously generates a partial genome sequence. (frontiersin.org)
Next-generation sequencing technologies have dramatically changed the approach to genetic diagnosis. (msdmanuals.com)
This revolutionary and rapidly evolving technology has moved a significant portion of the technical aspects of genetic diagnosis to next-generation sequencing and has become the mainstay of genetic diagnosis. (msdmanuals.com)

Sequence homology is an indication of the genetic relatedness of different organisms and gene function. (lookformedical.com)

These sequence datum have a significant impact to the research community studying fungal biology, field ecology, structural genomics, comparative genomics, and functional genomics. (usda.gov)

Therefore, we constructed a cDNA library using algal cells grown at exponential growth phase and carried out expressed sequence tag (EST) analysis. (academicconcepts.net)
was constructed in a λTriplEx2 vector by SMART TM cDNA library containing 2.37×10 6 independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. (zju.edu.cn)
A cDNA library of Chinese wild Vitis amurensis , which is the most cold-resistant species in the genus Vitis , was constructed using young leaves of seedlings of the clone Heilongjiang potted and subjected to cold stress. (geneticsmr.com)

In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)

Of 768 clone inserts submitted for differential screening by macroarray hybridization, 83% displayed a fourfold overexpression in the oocyte. (appliedbioinfo.com)

Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
Their findings suggest that co-expressed miRNAs collectively provide a systemic compensatory response to the abnormal phenotypic changes in cancer cells by targeting a broad range of functional categories and signaling pathways affected in a particular cancer. (biomedcentral.com)

DEFINITION Vibrio halioticoli IAM 14597 gene for 16S rRNA, partial sequence. (nig.ac.jp)
DEFINITION Sporobolomyces ruber JCM 6884 gene for 18S rRNA, partial sequence. (nig.ac.jp)

2] "The full-ORF clone resource of the German cDNA consortium. (tcdb.org)

Additionally, the 1.1 million WGP tags will support efficient assignment of sequence scaffolds to the BAC clones, and thus an efficient sequencing of BAC pools with targeted genome regions of interest. (frontiersin.org)

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. (lookformedical.com)
Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)

The high-quality physical map constructed in this study paves the way for the assembly of a reference sequence, from which breeding efforts will greatly benefit. (biomedcentral.com)

Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)
An analysis of 555 randomly chosen clones in the library showed that 80 were derived from abundant mRNAs and were accounted for by 29 distinct sequences. (diabetesjournals.org)
Gusev et al [ 22 ] report the results of a systems biology based analysis of aberrantly expressed miRNAs in five human cancers. (biomedcentral.com)
This study improves our understanding of the repetitive sequence organization of H. scandens genome and provides a basis for further analysis of their chromosome evolution process. (bvsalud.org)
Reverse transcription-PCR (RT-PCR) analysis demonstrated that antipathogenic T-phylloplanin-like proteins, glutathione peroxidase and several classes of pathogenesis-related (PR) proteins were expressed specifically or dominantly in trichomes. (uni-bayreuth.de)
BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. (zju.edu.cn)

In 1987 the sdhCAB operon of B. subtilis was cloned and sequenced. (lu.se)

This technology involves breaking the entire genome into small segments, sequencing the segments, and then reassembling the sequences using intensive computational techniques to provide the base-by-base sequence of the entire genome or more limited regions, such as the expressed portion of the genome known as the exome. (msdmanuals.com)

Highly repeated sequences, 6K-8K base pairs in length, which contain RNA polymerase II promoters. (lookformedical.com)
Highly repeated sequences, 100-300 bases long, which contain RNA polymerase III promoters. (lookformedical.com)

The coding sequence of SNAP-tag was cloned into a pTXB1 derived E. coli T7 expression vector. (neb.com)

When expressed in Xenopus laevis oocytes, hOAT3 mediated the transport of estrone sulfate ( K m = 3.1 μM), p -aminohippurate ( K m = 87.2 μM), methotrexate ( K m = 10.9 μM), and cimetidine ( K m = 57.4 μM) in a sodium-independent manner. (aspetjournals.org)

Segregating populations will be characterised with both single locus (microsatellites) and multi-locus (AFLPs) marker systems providing framework maps for the eventual location of cDNAs generated from single pass sequencing. (le.ac.uk)

The Alu sequence family (named for the restriction endonuclease cleavage enzyme Alu I) is the most highly repeated interspersed repeat element in humans (over a million copies). (lookformedical.com)
Multi-dimensional pooling of 295,680 BAC clones and sequencing the ends of restriction fragments of pooled DNA generated 1,814 million high quality reads, of which 825 million were deconvolutable to 1.11 million unique WGP sequence tags. (frontiersin.org)

Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
Upon i.m. injection of DNA vectors expressing Nef mut -derivatives, nanovesicles containing antigens fused with Nef mut are released by muscle cells, can freely circulate into the body, and can be internalized by antigen-presenting cells (APCs). (nature.com)
epithelial cells of the small intestine and kidney also express these enzymes to a lesser extent, but their contribution to urea production is not significant. (medscape.com)
check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)

A total of 6,528 clones were sequenced from this library generating 5,542 high-quality EST sequences. (nih.gov)

DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom. (lookformedical.com)

2 − 6 These nuclease-resistant polymers persist in live organisms for days and are typically designed to recognize 25-base sequences that span intron-exon junctions or translational start sites. (cdc.gov)

Anatomy13

Organisms32

Chemicals and Drugs34

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes82

Disciplines and Occupations4

Technology, Industry, Agriculture6

Information Science13

Health Care3

Genes24

Genomic7

ESTs10

CDNAs were cloned2

Repetitive Sequences4

Putative1

Characterization1

Nucleotide3

Chromosomes4

Homologous1

Contigs5

Genomes1

Nonredundant1

Mammalian2

Transgenic3

Amino1

Xylem1

Subtractive1

Binds1

Protein5

Zebrafish1

Gene expression1

Identical1

Next-generation4

Homology1

Fungal1

CDNA library3

Subunit1

Hybridization1

Functional2

Partial2

Consortium1

Genome regions1

Nucleic acids2

Assembly1

Analysis6

19871

Computational1

Polymerase2

Coli1

Oocytes1

Framework1

Restriction2

Cells4

Total1

Maize1

Resistant1