Clones proteins clone cells. Medical search. Frequent questions

Restricted T cell receptor expression by human T cell clones specific for mycobacterial 65-kDa heat-shock protein: selective in vivo expansion of T cells bearing defined receptors. (ox.ac.uk)
The diverse repertoire of T-cell receptors (TCR) plays a key role in the adaptive immune response to infections. (elifesciences.org)
A human ovarian small cell carcinoma line (BIN-67) expresses abundant calcitonin (CT) receptors (CTR) (143,000 per cell) that are coupled, to adenylate cyclase. (jci.org)
Transfection of this cDNA into COS cells resulted in expression of receptors with high affinity for salmon CT (Kd = approximately 0.44 nM) and for human CT (Kd = approximately 5.4 nM). (jci.org)
these receptors comprise a distinct family of G protein-coupled seven transmembrane domain receptors. (jci.org)

Forward primer to sequence targets cloned in expression vectors. (origene.com)

Twenty four to 48 hours after transfection begin selecting mammalian cultures in 600-1,200 µg/ml G418 (geneticin) depending on the cell line. (neb.com)
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal DDK-tag. (origene.com)

To clone a human CTR (hCTR), a BIN-67 cDNA library was screened using a cDNA probe from a porcine renal CTR (pCTR) that we recently cloned. (jci.org)
FAS was initially purified and cloned from SKW6.4 cells and a cDNA library of human T cell lymphoma KT-3 cells. (biolegend.com)
A cDNA clone coding for a membrane proteoglycan core protein was isolated from a neonatal rat Schwann cell cDNA library by screening with an oligonucleotide based on a conserved sequence in cDNAs coding for previously described proteoglycan core proteins. (rupress.org)

PYCR1 interference was able to inhibit cell proliferation and promote cell apoptosis of pancreatic cancer. (jcancer.org)

beta chain usage was not restricted since different clones expressed V beta 6.7, V beta 22.3 and V beta 12. (ox.ac.uk)
Because thousands of different clones exist, the body can produce a vast number of different antibodies to fight the numerous infectious microorganisms to which the body is exposed. (msdmanuals.com)

We have examined the T cell receptor (TcR) expression of clones specific for epitopes of mycobacterial 65-kDa heat-shock protein (hsp65) in the context of two different HLA molecules, and used this system as a model to assess the selection of T cells responsive to this antigen in vivo. (ox.ac.uk)
The resulting diverse naïve repertoire contains T-cell clones that recognize epitopes of yet unseen pathogens, and can participate in the immune response to infection or vaccination. (elifesciences.org)

Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. (jci.org)

The cloned cDNA encodes a putative peptide of 490 amino acids with seven potential transmembrane domains. (jci.org)
FAS is a type I transmembrane protein and belongs to the TNF receptor superfamily. (biolegend.com)

The genetic alterations in these clones include mutations, deletions, or insertions that affect both the constant and variable regions. (medscape.com)
Scope includes mutations and abnormal protein expression. (cancerindex.org)

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
The 397 amino acid recombinant protein has a predicted molecular mass of approximately 44.7 kD. (biolegend.com)

As a result, this clone produces vast amounts of a single antibody (monoclonal antibody) known as the M-protein. (msdmanuals.com)

Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
E1A transfection results in the transformation of peripheral lung epithelial cells and normal lung fibroblasts to a phenotype intermediate between that of the two primary cells. (ersjournals.com)
Reports from several laboratories suggest that a phenomenon termed epithelial-mesenchymal transformation, in which epithelial cells transform into fibroblasts capable of producing connective tissue matrix, is an important feature of tissue remodelling 10 , 11 . (ersjournals.com)
The changes that occur during adenoviral E1A transformation of primary guinea pig PLECs were also compared with those occurring during the transformation of the fibroblasts that frequently contaminate primary epithelial cultures, and evidence is presented that both cell types are transformed to an intermediate phenotype. (ersjournals.com)

Overexpression of R3052W in DLD1 parental BRCA2 wild-type cells confers sensitivity to MMC DNA damage. (frontiersin.org)
Overexpression of this gene is associated with tumor cell proliferation and metastasis. (cancerindex.org)
Transwell migration, cell cycle analysis, MTT and colony formation assays were performed to determine the effect of DRAM2 overexpression and knockdown on NSCLC-cell migration and proliferation. (cancerindex.org)
Additionally, DRAM2 overexpression promoted cell metastasis and proliferation in vitro, while knockdown of DRAM2 expression yielded opposite result. (cancerindex.org)
DRAM2 overexpression also increased proteins CDK4, CyclinD3, and decreased p27 expression, all of which are cell cycle-related factors. (cancerindex.org)

The heavy chain diseases (HCDs) are B-cell proliferative disorders characterized by production of abnormal, structurally incomplete, immunoglobulin heavy chains without the corresponding light chains. (medscape.com)
In γ-HCD, a mutant lymphoplasmacytoid cell clone synthesizes an abnormal IgG protein. (medscape.com)
The abnormal plasma cells and the antibodies they produce are limited to one type, and levels of other types of antibodies that help fight infections fall. (msdmanuals.com)
The ever-increasing number of abnormal plasma cells also invades and damages various tissues and organs, and the antibody produced by the clone of plasma cells can sometimes damage vital organs, especially the kidneys and bones. (msdmanuals.com)

We show that this united ExM (uniExM) protocol can be used to preserve and visualize RNA transcripts, proteins in biologically relevant ultrastructures, and sets of RNA transcripts in patient-derived xenograft (PDX) cancer tissues and may support the visualization of other kinds of biomolecular species as well. (plos.org)
uniExM may find many uses in the simple, multimodal nanoscale analysis of cells and tissues. (plos.org)
Nanoscale imaging enables the analysis of biological systems, such as cells and tissues, at the fundamental length scales of biomolecules and biomolecular interactions, but classically has required expensive equipment and advanced skillsets to perform, e . g ., via super-resolution microscopy [ 1 ]. (plos.org)
When the anti-core protein antibodies were used to stain tissue sections immunoreactivity was present in peripheral nerve, newborn rat brain, heart, aorta, and other neonatal tissues. (rupress.org)
B cells One of the body's lines of defense ( immune system) involves white blood cells (leukocytes) that travel through the bloodstream and into tissues, searching for and attacking microorganisms and. (msdmanuals.com)

This system includes all of the components needed for protein synthesis: a highly active cell extract, an optimized T7 RNA polymerase, an RNase inhibitor and a protein synthesis reaction buffer containing energy source, nucleotides, and amino acids. (neb.com)
Human FAS, amino acids (Arg17-Asn173) (Accession# NM_000043), was expressed in 293E cells. (biolegend.com)

Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. (origene.com)
The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. (cancerindex.org)
What does this gene/protein do? (cancerindex.org)
Adenoviral DNA is retained by peripheral epithelial cells of the lung (PLECs) in humans in whom the viral E1A gene is expressed 4 without replication of a complete virus 5 . (ersjournals.com)

Expression of the fusion protein cloned in pSNAP-CaaX can be achieved by transiently transfecting cells in culture with standard transfection protocols. (neb.com)

Using TCR alpha and beta repertoire sequencing for T-cell subsets, as well as single-cell RNAseq and TCRseq, we track the concentrations and phenotypes of individual T-cell clones in response to primary and secondary yellow fever immunization - the model for acute infection in humans - showing their large diversity. (elifesciences.org)

There was conservation of alpha chain usage, since all clones expressed a member of the V alpha 1 family, but again CDR3 sequence conservation was not apparent. (ox.ac.uk)
Estimating the fraction of the T-cell response directed against the single immunodominant epitope, we identify the sequence features of TCRs that define the high precursor frequency of the two major TCR motifs specific for this particular epitope. (elifesciences.org)
Interestingly, the hCTR sequence is remotely related to the cAMP receptor of Dictyostelium discoideum (21% identical), but is not significantly related to other G protein-coupled receptor sequences now in the data bases. (jci.org)
V2_F Primer can be used to sequence the target sequence after cloning. (origene.com)

(A) BRCA2 protein schematic depicting domain organization: N-terminus, BRC repeats DNA binding domain (DBD), and C-terminal domain (CTD). (frontiersin.org)
(C) Clonogenic survival analyses of BRCA2 WT versus the two independent R3052W clones after treatment with Olaparib, cisplatin, and mitomycin C. (D) Schematic of I-SceI nuclease-induced DSB HDR luciferase assay. (frontiersin.org)

E1A-PLECs and E1A-NLFs positive for E1A DNA, mRNA and protein expressed cytokeratin and vimentin but not smooth muscle α-actin. (ersjournals.com)
On Northern blots the cDNA hybridized to a single 5.6-kb mRNA that was present in Schwann cells, neonatal rat brain, rat heart, and rat smooth muscle cells. (rupress.org)
A ribonuclease protection assay was used to quantitate levels of the core protein mRNA. (rupress.org)

The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). (origene.com)
pSNAP-CaaX has performed well in stable and transient transfection of CHO-K1 and NIH 3T3 cells. (neb.com)

Fas-activated DCs increase the expression of MHC II, B7 co-stimulatory molecules, and DC-lysosome-associated membrane proteins (DC-LAMP), and secrete proinflammatory cytokines such as IL-1β and TNF-α. (biolegend.com)

Combine the following in a 1.5 ml microcentrifuge tube: 12 µl of NEBExpress S30 Synthesis Extract, 25 µl of 2X Protein Synthesis Buffer, 1µl of T7 RNA Polymerase, 1 µl of RNase Inhibitor, 250 ng of DNA template, and nuclease free water to total reaction volume of 50 µl. (neb.com)
Primer extension and polymerase chain reaction amplification were used to obtain additional 5' protein coding sequences. (rupress.org)

Sequencing alpha and beta chains of representative V beta 5.2+ and V beta 5.6+ PB-derived clones revealed TcR which were identical to those utilized by the SF-derived clones, showing that the repertoire for recognition of this antigen is stable over time. (ox.ac.uk)

Similar studies of TcR expression were carried out on hsp65-specific, DP4-restricted clones derived from the SF of a patient with rheumatoid arthritis by two independent cloning procedures. (ox.ac.uk)
This clone was engineered to express the complete ORF with an expression tag. (origene.com)
DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53. (cancerindex.org)
Western blotting confirmed the expression of DRAM2, p53, and the other involved proteins. (cancerindex.org)
It is possible to use pools of stable cell populations for initial cell labeling to test for the presence of SNAP-tag expression. (neb.com)
These two cell types differed, however, in their NF-κB and AP-1 binding after lipopolysaccharide stimulation, possibly due to differences in the expression of the subunits that comprise these transcriptional complexes. (ersjournals.com)
RNA interference was used to inhibit the expression of PYCR1 in PANC- 1 and AsPC-1 cells. (jcancer.org)
After infection, the expression of PYCR1 protein was detected by Western blot. (jcancer.org)
We show that CGS21680 raises lysosomal calcium levels and rescues mitochondrial functionality (mitochondrial inner membrane potential and expression of the complex IV of the mitochondrial respiratory chain), which is compromised in NPC1 cells. (jneurosci.org)

In addition, FAS/FASL interaction increases the production of chemokines in NK-T cells, and FASL acts as a survival factor for human CD34 + cells and increases their colony formation. (biolegend.com)

Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB ® has the solution for you. (neb.com)
Assuming a few years to find suitable mammoth DNA and adapt Wakayama's techniques for this experiment, and 600 days of gestation once the clone is implanted in an adult African elephant, we could have a mammoth birth in five years or so. (singularityhub.com)

T-cells play a crucial role in the immune response to pathogens by mediating antibody formation and clearance of infected cells, and by defining an overall response strategy. (elifesciences.org)
The resulting group of genetically identical cells (called a clone) produces a large quantity of a single type of antibody (immunoglobulin). (msdmanuals.com)
The cells of a clone produce only one specific type of antibody. (msdmanuals.com)

Furthermore, the effect of PYCR1 interference on tumor growth was evaluated in vivo through injecting tumor cells subcutaneously into nude mice. (jcancer.org)

The NEBExpress Cell-free E.coli Protein Synthesis System can synthesize protein in just 2 to 4 hours. (neb.com)
Once the template DNA is purified, thaw the NEBExpress Cell-free E. coli Protein Synthesis System components on ice. (neb.com)

Labeling of transfected cells with a fluorescent SNAP-Cell substrate should show strong inner cell membrane fluorescence. (neb.com)

At a time when the idea that cancer had a genetic basis was widely disbelieved, Nowell's results provided the first clear evidence that a particular genetic defect in a single chromosome can lead to a population or clone of identical cells that accumulate in numbers to form a deadly malignancy. (laskerfoundation.org)

The alpha chains expressed by V beta 5.2+ and V beta 5.6+ clones were from different families, V alpha 2.4 and V alpha 23.2, respectively. (ox.ac.uk)
Once more, T cell clones with identical alpha and beta TcR chains were obtained from the separate cloning procedures, suggesting oligoclonalty of T cells with this defined specificity in the patient's SF. (ox.ac.uk)
The specificity of T-cells is determined by the T-cell receptor (TCR), a heterodimer of alpha and beta protein chains. (elifesciences.org)

The BRCA2 R3052W mutated protein exacerbates genome instability, is unable to rescue homology-directed repair, and fails to complement cell survival following exposure to PARP inhibitors and crosslinking drugs. (frontiersin.org)
(G) Clonogenic survival analyses of DLD parental clones upon treatment with mitomycin C. (frontiersin.org)

The proliferation and growth of PDAC cells were detected by Celigo analysis, MTT, and clone formation assay. (jcancer.org)

A 16-kD protein fragment encoded by the cDNA was expressed in bacteria and used to immunize rabbits. (rupress.org)

The resulting antibodies reacted on immunoblots with the core protein of a detergent extracted heparan sulfate proteoglycan. (rupress.org)

Surprisingly, despite anticipated defects in DNA binding or RAD51-mediated DNA strand exchange, the BRCA2 R3052W protein mislocalizes to the cytoplasm precluding its ability to perform any DNA repair functions. (frontiersin.org)

Subtle differences in epitope specificity were detected for two clones with differing TcR. (ox.ac.uk)

These proteins help the body fight infection. (msdmanuals.com)

The tiny Philadelphia chromosome became a clear and consistent marker of CML, a cancer of the myeloid or bone marrow cells, with broad implications for diagnosis and prognosis of disease. (laskerfoundation.org)

Gently vortex the NEBExpress S30 Synthesis Extract and Protein Synthesis Buffer to mix. (neb.com)
Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. (biolegend.com)

Cell apoptosis was analyzed by flow cytometry. (jcancer.org)

Plasma cell disorders are uncommon. (msdmanuals.com)
In plasma cell disorders, one clone of plasma cells multiplies uncontrollably. (msdmanuals.com)
Thus, people with plasma cell disorders are often at higher risk of infections. (msdmanuals.com)

The system routinely produces approximately 25 μg of protein per 50 μl reaction, or 1/2 mg/ml. (neb.com)

This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. (origene.com)
Multiple alternatively spliced variants, encoding the same protein, have been identified. (cancerindex.org)

Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. (biolegend.com)

The synthesized protein can be isolated by affinity purification, for example, using NEBExpress Ni-NTA magnetic beads or NEBExpress Ni Spin columns for further analysis. (neb.com)

The core protein had an apparent mass of 120 kD. (rupress.org)

Plasma cells are present mainly in bone marrow and lymph nodes. (msdmanuals.com)

Therefore, we aimed to explore the effect of PYCR1 on the growth of PDAC cells. (jcancer.org)

The dissociation constants (Kd) for the CTRs on these BIN-67 cells is approximately 0.42 nM for salmon CT and approximately 4.6 nM for human CT. (jci.org)
Inhibition of cytotoxic effect of FASL on Jurkat cells in the presence of recombinant human FAS. (biolegend.com)

α-HCD frequently presents as an extranodal marginal-zone lymphoma of the mucosa-associated lymphoid tissue (MALT), considered a variant of MALT lymphoma, and is the only HCD that can transform into diffuse large B-cell lymphoma. (medscape.com)

It is recommended that you establish a kill curve for each cell line to determine optimal selection conditions. (neb.com)

Here we present a multifunctional anchor, an acrylate epoxide, that enables proteins and RNAs to be equipped with anchors in a single experimental step. (plos.org)

(B) Western blot of total cellular lysates from DLD-1 BRCA2 −/− cells stably transfected with full-length BRCA2 cDNA constructs: BRCA2 Wild Type (WT) and BRCA2 R3052W (1 and 2 correspond to two independent clones). (frontiersin.org)
(F) Western blot of total cellular lysates from DLD-1 parental cells (these cells express a wild-type allele of BRCA2) stably transfected with R3052W (3 and 5 correspond to two independent clones) full-length 2XMBP-BRCA2 cDNA constructs. (frontiersin.org)

Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. (cancerindex.org)

μ-HCD clinically resembles small cell lymphoma or chronic lymphocytic leukemia. (medscape.com)
Nowell collaborated with the late David Hungerford who, he says, "knew more about chromosomes than I did," and together they made the startling observation that individuals suffering from chronic myelogenous leukemia (CML) had an abnormally small chromosome in the tumor cells. (laskerfoundation.org)

The Coulter® MAXM performs a complete blood count on blood specimens to provide a study of blood cells and coagulation for all participants. (cdc.gov)

Here, we show that DRAM2 may act as an oncogenic regulator in non-small cell lung cancer (NSCLC). (cancerindex.org)

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