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  • vitro
  • The transfection of hammerhead ribozyme or the addition of chemically modified ribozyme against the flanking region of V3-7 caused a potent and selective inhibition of anti-DNA production in V3-7-using B cell clones, but not in irrelevant V gene-using clones in vitro. (jimmunol.org)
  • These T cell clones proliferated in vitro in response to B. gingivalis, but not to other bacteria (Prevotella intermedia, Actinobacillus actinomycetemcomitans, Wolinella recta, Fusobacterium nucleatum, Streptococcus sanguis). (nii.ac.jp)
  • Two T-cell clones associated with GVM were isolated and expanded in vitro , allowing their phenotypic and functional characterization. (aacrjournals.org)
  • As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. (wikipedia.org)
  • Clonal
  • Here we determine the degree of hematopoietic migration at a clonal level by transplanting individual young and aged mouse HSCs labeled with barcoded viral vector, followed by assessing the skeletal distribution of hundreds of HSC clones. (rupress.org)
  • Molecular analysis of CDR3 Vβ repertoire after CD4+ DLI demonstrated previously that the development of GVM and graft- versus -host-disease (GVHD) were associated with the clonal expansion of distinct T-cell populations in patient peripheral blood. (aacrjournals.org)
  • demonstrate
  • Importantly, accumulating studies demonstrate that cytotoxic CD4 T cells develop following DENV infections and may play a crucial role in protecting the host from severe dengue disease. (frontiersin.org)
  • tissues
  • Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. (abgent.com)
  • transcriptomes
  • We hypothesized that key disease-associated cells would show evidence of prior Ag exposure, inferred from expanded TCR clonotypes, and essential phenotypic properties in their transcriptomes. (jimmunol.org)
  • A comprehensive study of the genomes and transcriptomes of HEK 293 and five derivative cell lines compared the HEK 293 transcriptome with that of human kidney, adrenal, pituitary and central nervous tissue. (wikipedia.org)
  • gene
  • V gene analysis also revealed that the V3-7 or VH4-21 gene, which encodes nephritogenic anti-DNA Ab O-81 or NE-1, was preferentially used for IgG anti-DNA autoantibodies or IgG anti-acetylcholine receptor Abs associated with autoimmune diseases, but not for the Abs to exogenous Ags ( 7 , 8 , 9 , 10 ). (jimmunol.org)
  • Thus
  • Viruses offer an efficient means of delivering genes into cells, which they evolved to do, and are thus of great use as experimental tools. (wikipedia.org)
  • This danger can be avoided by the use of viruses which lack key genes, and which are thus unable to replicate after entering a cell. (wikipedia.org)
  • laboratory
  • Recent work from our laboratory identified T cell crossreactivity between epitopes of OspA and lymphocyte function-associated antigen 1alpha(L) chain (LFA-1alpha(L)) in these patients. (stanford.edu)
  • HEK 293 cells were generated in 1973 by transformation of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb's laboratory in Leiden, the Netherlands. (wikipedia.org)
  • copies
  • HEK 293 cells have a complex karyotype, exhibiting two or more copies of each chromosome and with a modal chromosome number of 64. (wikipedia.org)
  • human
  • Human embryonic kidney cells 293, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. (wikipedia.org)
  • Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. (wikipedia.org)
  • Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells. (wikipedia.org)
  • protein
  • Examples of such experiments include: Effects of a drug on sodium channels Inducible RNA interference system Isoform-selective protein kinase C agonist Interaction between two proteins Nuclear export signal in a protein A more specific use of HEK 293 cells is in the propagation of adenoviral vectors. (wikipedia.org)
  • However
  • However, it is now evident that CD4 T cells can also perform cytotoxic functions and induce the apoptosis of target cells. (frontiersin.org)
  • However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 line may have been unusual in some fashion. (wikipedia.org)
  • function
  • We review our current understanding of the differentiation and function of cytotoxic CD4 T cells, with a focus on DENV infection, and discuss the potential of harnessing these cells for the prevention and treatment of DENV infection and disease. (frontiersin.org)
  • either
  • For many years it was assumed that HEK 293 cells were generated by transformation of either a fibroblastic, endothelial or epithelial cell, all of which are abundant in kidneys. (wikipedia.org)