AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaInformation ScienceHealth Care
Clone CellsCloning, MolecularMolecular Sequence DataBase SequenceAmino Acid SequenceDNA, ComplementaryDNASequence Analysis, DNANucleic Acid HybridizationSequence Homology, Nucleic AcidRNA, MessengerCell LinePolymerase Chain ReactionGenesSequence Homology, Amino AcidChromosomes, Artificial, BacterialBlotting, SouthernChromosome MappingGenomic LibraryPhylogenyBlotting, NorthernPlasmidsDNA Restriction EnzymesTransfectionDNA, BacterialCosmidsDNA, RecombinantSequence AlignmentMutationTranscription, GeneticGene ExpressionDNA PrimersElectrophoresis, Gel, Pulsed-FieldEscherichia coliPhenotypeT-LymphocytesGenes, BacterialEpitopesHybrid CellsGenetic VariationOpen Reading FramesTumor Cells, CulturedRecombinant ProteinsAntibodies, MonoclonalDNA FingerprintingRepetitive Sequences, Nucleic AcidCell DivisionCells, CulturedT-Lymphocytes, CytotoxicGenetic VectorsSpecies SpecificityLymphocyte ActivationSequence Tagged SitesGene Expression RegulationRNADNA, ViralDNA ProbesContig MappingMultigene FamilyOligonucleotide ProbesPolymorphism, Restriction Fragment LengthGenotypeChromosomes, Artificial, YeastMolecular WeightBacterial Typing TechniquesPoly AExpressed Sequence TagsCricetinaeVirus ReplicationGenes, ViralReceptors, Antigen, T-CellMolecular EpidemiologyMice, Inbred BALB CPeptide LibraryBacterial ProteinsAntigens, SurfaceProtein BiosynthesisRNA, Ribosomal, 16SReceptors, Antigen, T-Cell, alpha-betaIn Situ Hybridization, FluorescenceB-LymphocytesCell DifferentiationKaryotypingRNA, ViralSerotypingCloning, OrganismCD4-Positive T-LymphocytesChickensExonsCell Line, TransformedCluster AnalysisDNA Transposable ElementsMultilocus Sequence TypingGenetic Complementation TestCross ReactionsRecombinant Fusion ProteinsDNA, PlantPlant ProteinsGenetic MarkersReverse Transcriptase Polymerase Chain Reaction
ProliferationVivoInhibitFetalStromal cellsGenesBiolExtracellularEmbryonicPredominantlyLineageMyeloid CellsMethodsAntibodyEosinophilsEndothelialEndocytosisImmatureCytotoxicityProteinsMalignantLymphocytesProteinInhibitoryHumanReceptorFunctionalStem cellDifferentiationStructuresTypesInflammatoryFoundEarlyProductsCancerBloodInductionPrimaryProcessesSingle cellSpecific
Proliferation3
  • MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo . (rndsystems.com)
  • Transcription factor CHF1/Hey2 regulates neointimal formation in vivo and vascular smooth muscle proliferation and migration in vitro. (jax.org)
  • The lectin was found to inhibit in vitro proliferation of human cancer cell lines HT29, SiHa and OVCAR-5. (lookformedical.com)
Vivo4
  • Importantly, the in vivo expression of the lacZ fusion products accurately recapitulated the observed in vitro expression patterns. (ox.ac.uk)
  • Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
  • STAT5 activation seems particularly important for JAK2V617F -induced cytokine independence in vitro and erythrocytosis in vivo and is in itself sufficient to support formation of endogenous erythroid colonies, a cardinal feature of PV. (oncohemakey.com)
  • To investigate the structural organisation, dynamics and functional importance of F-actin in endothelial cells (ECs) during angiogenesis in vivo , we generated a transgenic zebrafish line expressing Lifeact-EGFP in ECs. (biologists.com)
Inhibit1
  • The DX12 antibody can reportedly inhibit spontaneous cytotoxicity mediated by certain NK cell clones. (bdbiosciences.com)
Fetal2
  • LYAR is present at high levels in early embryos and preferentially in the liver fetal thymus. (wikipedia.org)
  • Reports indicate that CD161 is expressed preferentially on CD45RO+ T cells, however, it can be found on a subset of thymocytes and fetal liver T cells. (bdbiosciences.com)
Stromal cells3
  • Using two different gene trap vectors, we have isolated embryonic stem (ES) cell clones containing lacZ reporter gene insertions in genes expressed in blood island and vascular cells, muscle, stromal cells, and unknown cell types. (ox.ac.uk)
  • Finally, in situ hybridization analyses revealed the presence of RP11-295G20.2 in the nuclei of corneal epithelial cells, but not in the stromal cells of the human cornea, while RP11-322M19.1 was present in epithelial and non-epithelial cells. (molvis.org)
  • Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
Genes7
  • Expression trapping: identification of novel genes expressed in hematopoietic and endothelial lineages by gene trapping in ES cells. (ox.ac.uk)
  • Expression and sequence analysis of representative clones suggest that this approach will be useful for identifying and mutating novel genes expressed in the developing hematopoietic and vascular systems. (ox.ac.uk)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • Characterization of the human and mouse HEY1, HEY2, and HEYL genes: cloning, mapping, and mutation screening of a new bHLH gene family. (jax.org)
  • The basic helix-loop-helix genes Hesr1/Hey1 and Hesr2/Hey2 regulate maintenance of neural precursor cells in the brain. (jax.org)
Biol1
Extracellular1
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Embryonic3
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Predominantly1
  • Results We discovered that neoantigen-specific TIL clones were predominantly CD4 + T cells and were present in both therapeutic responders and non-responders. (bmj.com)
Lineage2
  • Pre- hematopoietic and other cells, but the Duffy sence of one copy of the deleted CCR5 gene also antigen of erythrocytes (DARC) is the only influences the course of disease as the onset of member expressed on cells of erythroid lineage. (cdc.gov)
  • Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Myeloid Cells1
  • The chronic myeloproliferative neoplasms (MPNs) are clonal disorders characterized by overproduction of mature myeloid cells. (oncohemakey.com)
Methods8
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
  • The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
  • Methods We analyzed infused TIL products from metastatic melanoma patients previously treated with ACT for the presence of neoantigen-specific T cells. (bmj.com)
  • Conclusions Despite methods which often focus on CD8+T cells, our study supports the importance of prospective identification of neoantigen-specific CD4 + T cells within TIL products as they are a potent source of tumor-specific effectors. (bmj.com)
Antibody3
  • Cell growth-regulating nucleolar protein is a protein that in humans is encoded by the LYAR gene (Ly-1 antibody reactive clone). (wikipedia.org)
  • Since its discovery in 2000, initial in vitro studies have found that the engagement of Siglec-8 with a monoclonal antibody or with selective polyvalent sialoglycan ligands induced the cell death of eosinophils and inhibited mast cell degranulation. (mdpi.com)
  • The DX12 monoclonal antibody specifically binds to human CD161 which is also known as Natural killer cell surface protein P1A (NKR-P1A). (bdbiosciences.com)
Eosinophils2
  • MIP-1 alpha has also been shown to attract B cells as well as eosinophils. (rndsystems.com)
  • Among these, Siglec-8 is a CD33-related family member selectively expressed on human mast cells and eosinophils, and at low levels on basophils. (mdpi.com)
Endothelial2
  • During angiogenesis, actin-rich filopodia protrusions have been proposed to drive endothelial tip cell functions by translating guidance cues into directional migration and mediating new contacts during anastomosis. (biologists.com)
  • These results reveal that the induction of tip cells and the integration of endothelial guidance cues do not require filopodia. (biologists.com)
Endocytosis1
  • Actin filaments are instrumental in driving processes such as migration, cytokinesis and endocytosis and provide cells with mechanical support. (biologists.com)
Immature2
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • Bennett IM, Zatsepina O, Zamai L, Azzoni L, Mikheeva T, Perussia B. Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiates in vitro in the presence of interleukin 12. (bdbiosciences.com)
Cytotoxicity1
Proteins5
  • The macrophage inflammatory proteins -1 alpha and -1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. (rndsystems.com)
  • Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8 + and CD4 + T cells, respectively. (rndsystems.com)
  • The results have shown that DDB2 Wt recognize and repair the UV-induced lesions in plasmidic DNA transfected in the cells, whereas a delay in these processes were observed in the presence of DDB2 PCNA- , as also confirmed by the different extent of co-localization of DDB2 Wt and some NER proteins (such as XPG), vs the DDB2 mutant form. (biomedcentral.com)
  • Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
  • While all coronaviruses contains these proteins, a subset (HEV, HCV-OC43 and BCV) is now recognized to possess an additional glycopolypeptide (gp65), which is unrelated to S or M. N protein The number of amino acids in the N protein has been determined by cloning and sequencing for MHV strains A59 (Armstrong et al. (pdfroom.com)
Malignant2
  • Among sporadic endometrial cancers, when present, the microsatellite instability is confined to the malignant cells, and is not seen in the adjacent normal epithelium. (medmuv.com)
  • Also, the term "primary cutaneous CD4 + small/medium T-cell lymphoma" was changed to "primary cutaneous CD4 + small/medium T-cell lymphoproliferative disorder" because of its indolent clinical behavior and uncertain malignant potential. (medscape.com)
Lymphocytes2
  • Background Adoptive cell therapy (ACT) with tumor-infiltrating lymphocytes (TILs) is a promising immunotherapeutic approach for patients with advanced solid tumors. (bmj.com)
  • Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)
Protein2
  • Previously we have shown that DDB2, a protein involved in the Global Genome Repair, interacts directly with PCNA and, in human cells, the loss of this interaction affects DNA repair machinery. (biomedcentral.com)
  • To clarify this issue, we decided to apply a transfection-based assay, named Host Cell Reactivation (HCR), to investigate DNA lesions removal efficacy in the presence of DDB2 Wt protein or DDB2 mutated one. (biomedcentral.com)
Inhibitory1
  • Siglecs (sialic acid-binding immunoglobulin-like lectins) are single-pass cell surface receptors that have inhibitory activities on immune cells. (mdpi.com)
Human8
  • Human MIP-1 alpha refers to the products of several independently cloned cDNAs, including LD78, pL78, pAT464, and GOS19. (rndsystems.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • Purification and characterization of a lectin from Arisaema tortuosum Schott having in-vitro anticancer activity against human cancer cell lines. (lookformedical.com)
  • A lectin with in-vitro anticancer activity against established human cancer cell lines has been purified by affinity chromatography on asialofetuin-linked amino activated silica beads from the tubers of Arisaema tortuosum, popularly known as Himalayan Cobra lily, a monocot plant from the family Araceae. (lookformedical.com)
  • Moreover, in human studies, JAK2V617F has been identified in the stem cell-enriched CD34 + CD38 − CD90 + Lin − compartment, common myeloid progenitors, granulocyte-monocyte precursors, and megakaryocyte-erythroid precursors as well as natural killer cells, B cells, and T cells in some patients, confirming its origin in an early hematopoietic progenitor. (oncohemakey.com)
  • The Host Cell Reactivation assay (HCR) allows studying the DNA repair capability in different types of human cells. (biomedcentral.com)
  • To this end, human kidney 293 stable clones, expressing DDB2 Wt or DDB2 PCNA- , were co-transfected with pmRFP-N2 and UV-irradiated pEGFP-reported plasmids. (biomedcentral.com)
  • This method allows studying the DNA repair capability in different types of human cells [ 11 ] and may be employed as a marker for genetic instability and cancer risk [ 12 , 13 ]. (biomedcentral.com)
Receptor3
  • One major discovery was that members of the chemokine receptor family serve as cofactors for HIV entry into cells. (cdc.gov)
  • CD161 is encoded by the KLRB1 (Killer cell lectin-like receptor subfamily B member 1) gene. (bdbiosciences.com)
  • Its function has not been fully elucidated, but reports indicate that NKR-P1A may serve as a specific receptor for some NK cell targets. (bdbiosciences.com)
Functional2
  • Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
  • For functional analysis, we used low concentrations of Latrunculin B that preferentially inhibited F-actin polymerisation in filopodia. (biologists.com)
Stem cell1
  • Mutations in TET2, other epigenetic regulators, and other regulators of cytokine signaling are not specific to the classic myeloproliferative neoplasms (MPNs) but may influence prognosis and play roles in hematopoietic stem cell (HSC) dysregulation and progression to accelerated or blast-phase disease. (oncohemakey.com)
Differentiation4
  • The majority of ES clones that expressed lacZ in blood islands also expressed lacZ upon differentiation into hematopoietic cells on OP9 stromal layers. (ox.ac.uk)
  • Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Structures2
  • Live imaging identifies dynamic and transient F-actin-based structures, such as filopodia, contractile ring and cell cortex, and more persistent F-actin-based structures, such as cell junctions. (biologists.com)
  • Respecting the preferentially intracellular localization of sGAG in vesicle-like structures, also the interaction data indicate sGAG-specific modulation of vesicle-based transport processes. (degruyter.com)
Types2
  • In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
  • The MPNs comprise a set of clonal HSC disorders, characterized by the overproduction of 1 or more mature myeloid cell types. (oncohemakey.com)
Inflammatory1
  • These cells can participate in inflammatory responses by releasing mediators that attract or activate other cells, contributing to the pathogenesis of allergic and non-allergic diseases. (mdpi.com)
Found4
  • While no mutations were found in the exons of the DCC gene in endometrial cancers, chromosome 18 was found to be capable of suppressing tumorigenicity of endometrial cancer cells in nude mice by the procedure of microcell fusion, with DCC expression elevated in most of the suppressed hybrids. (medmuv.com)
  • Genomic sequence of ala was also cloned using genomic walker technology, and it was found to contain three putative TATA boxes and eight possible CAAT boxes in the 5'-flanking region. (lookformedical.com)
  • Expression pattern analysis revealed that the ala preferentially expressed in the tissues with the higher expression being found in spadix, bud, leaf, spathe and tuber. (lookformedical.com)
  • The activating effects of the mutation are thought to reflect disruption of the normal autoinhibitory function of the JH2 domain, within which JAK2V617F is found, and have been shown to affect downstream pathways, including STATs (especially STAT5), phosphatidylinositol 3-kinase, and MAP kinase pathways in cell lines and animal models. (oncohemakey.com)
Early2
  • Since the early years of the HIV epidemic, cell function, have been suggested to explain significant differences in the rate of disease these findings (7,10,11,15). (cdc.gov)
  • Early patch-stage cutaneous T-cell lymphoma. (medscape.com)
Products1
  • While numerous advances have been made, the contribution of neoantigen-specific CD4 + T cells within TIL infusion products remains underexplored and therefore offers a significant opportunity for progress. (bmj.com)
Cancer1
  • Although this most common gynecologic cancer carries the best prognosis, it is important to differentiate between the classic endometrioid tumors that tend to be estrogen dependent and well differentiated, from other less common high-risk uterine malignancies, such as uterine papillary serous carcinomas (UPSCs), clear cell carcinomas, mixed mullerian tumors (MMTs), or sarcomas, which appear to have a different biology. (medmuv.com)
Blood1
  • In PV, bone marrow panmyelosis is associated with increased numbers of red blood cells and, in some patients, neutrophilia and/or thrombocytosis. (oncohemakey.com)
Induction1
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
Primary2
  • HERP1 is a cell type-specific primary target of Notch. (jax.org)
  • Among the changes to CTCL classification were the addition of primary cutaneous acral CD8 + T-cell lymphoma as a new provisional entity. (medscape.com)
Processes1
  • The mutagenic effect of UV is efficiently neutralized by DNA repair processes involving not only GG-NER but also the transcription-coupled nucleotide excision repair (TC-NER), a sub-pathway that preferentially removes DNA lesions generated in highly transcribed DNA regions [ 7 ]. (biomedcentral.com)
Single cell1
  • These results were validated by paired TCR and single cell RNA sequencing, which elucidated transcriptomic profiles distinct to neoantigen-specific CD4 + TIL. (bmj.com)
Specific2
  • Knockdown of PNN in HCET cells led to specific changes in the inclusion of multiple cassette exons as well as in the use of alternative splice sites in RP11-322M19.1 and RP11-18I14.1, resulting in considerable net changes in the ratio between the splice variants. (molvis.org)
  • thus, Pnn may affect crucial alternative splicing (AS) decisions and, in turn, impact cell-type specific gene expression. (molvis.org)