Clones gene deletion. Medical search. Frequent questions

Many of the deletion mutations are sold by the biotech firm Invitrogen. (wikipedia.org)
X linked Alport syndrome (ATS, OMIM 301050) is a hereditary glomerulonephritis resulting from either point mutations or intragenic deletions of the COL4A5 gene encoding the α5 chain of type IV collagen. (bmj.com)
Ironically, as both papers point out, it might be the case that the DNA repair defects caused by mutation of BRCA2 increase the likelihood of mutations that lead to the deletion of the 6174delT frameshift and drug resistance. (nature.com)
Synthetic lethality or sickness by double gene knockout mutations is one of the most powerful methods for analyzing robustness. (cam.ac.uk)
One patient at the TMD stage revealed 2 clonal expansions with different GATA1 mutations, of which 1 clone had an additional driver mutation. (unige.ch)
Interestingly, it was the other clone that gave rise to AMKL after accumulating mutations in 7 other genes. (unige.ch)
At least four mutations in the SMARCAD1 gene have been found to cause adermatoglyphia, which is the absence of dermatoglyphs on the hands and feet. (medlineplus.gov)
Nousbeck J, Sarig O, Magal L, Warshauer E, Burger B, Itin P, Sprecher E. Mutations in SMARCAD1 cause autosomal dominant adermatoglyphia and perturb the expression of epidermal differentiation-associated genes. (medlineplus.gov)
Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. (whispermovie.com)
in 2016, a tenth type was defined based on mutations in the AP3D1 gene. (medscape.com)
Gene mutations: missense versus nonsense mutations, insertions, deletions and frameshifts. (manchester.ac.uk)
used genome editing to make mutations in a stretch of DNA that regulates the gene involved in wing vein formation. (elifesciences.org)
The effects of these mutations unexpectedly suggested that pairs of chromosomes might be interacting to control the activity of this gene. (elifesciences.org)
Nonsense mutations of the CYBB gene in two Thai families with X-linked chronic granulomatous disease. (lu.se)
Characterization of 17 new cases of X-linked chronic granulomatous disease with seven novel mutations in the CYBB gene. (lu.se)
Characterization of six novel mutations in the CYBB gene leading to different sub-types of X-linked chronic granulomatous disease. (lu.se)
Myelodysplastic syndromes are a group of clonal hematopoietic stem cell disorders unified by the presence of distinct mutations of hematopoietic stem cells, most frequently in genes involved in RNA splicing. (msdmanuals.com)
Loss of expression of the respec- rectal cancers [4,5] and is known to tive mismatch repair genes protein arise due to mutations in DNA mis- Tissue blocks of 32 different patients was defined as absence of nuclear match repair genes [6]. (who.int)
Mismatch repair proteins correct King Khalid University Hospital, King mal nuclear staining in lymphocytes the insertion and deletion mutations Saud University, Riyadh, Saudi Arabia and normal epithelial or stromal cells that occur when DNA is copied be- between 2007 and 2009 were retrieved was required serving as internal con- fore cell division. (who.int)

The yeast deletion project, formally the Saccharomyces Genome Deletion Project, is a project to create data for a near-complete collection of gene-deletion mutants of the yeast Saccharomyces cerevisiae. (wikipedia.org)
We are also developing tools for efficient conjugation for combining different single deletions to make double knockout mutants. (cam.ac.uk)
Analysis of 5' promoter deletion mutants revealed that the region from -441 to +118, relative to the transcription initiation site, is sufficient to establish high level promoter activity. (nih.gov)
Cfhos2 deletion mutants were mildly reduced in vegetative growth rate, but almost lost pathogenicity on apple leaves. (biomedcentral.com)
Cfhos2 deletion mutants induced strong plant defense responses manifested by epidermal cell browning, granulation, and distortion of pathogen invasive hyphae. (biomedcentral.com)
Moreover, the expression of an apple defense-related F-box protein was strongly induced by infection with Cfhos2 deletion mutants. (biomedcentral.com)

Each strain carries a precise deletion of one of the genes in the genome. (wikipedia.org)
A PCR-based gene deletion strategy was used to generate deletions of each ORF in the yeast genome. (biocat.com)
Genome-wide association study identifies a maternal copy-number deletion in PSG11 enriched among preeclampsia patients. (nih.gov)
Students will understand the basis of genome maintenance and gene expression, and appreciate the concept of mutation at the level of the genotype and how this manifests at the phenotypic level. (manchester.ac.uk)
One of the most important points about genome-wide association studies is that they're (more or less) unbiased-- that is, you don't have to think about which genes could be involved in the phenotype before studying it. (gnxp.com)
I'm in the latter group, as a strong signal in a genome-wide association can in some cases lead to new candidate genes, new hypotheses and expose interesting biology. (gnxp.com)
Overlapping cDNA fragments of the DK-3592B genome were cloned after RT-PCR amplification, and their DNA sequenced by the di-deoxy chain termination method. (biomedcentral.com)
A full-length cDNA copy (pVHSVdk) of the DK-3592B strain genome was constructed by assembling six overlapping cDNA fragments by using natural or artificially created unique restriction sites in the overlapping regions of the clones. (biomedcentral.com)
Complete sequence of the VHSV DK-3592B genome was determined from the cloned cDNA and deposited in GenBank under the accession no. (biomedcentral.com)
Quintara offers a complete package for stable cell line and iPSC clone development with gene deletion, gene mutation, and precise gene writing at desired genome locations. (quintarabio.com)
In 2015, researchers reported that they had used components of the CRISPR/Cas9 genome editing system to edit genes so that they could propagate in a "Super-Mendelian" fashion. (elifesciences.org)
including the two researchers involved in the 2015 work - have used a new active genetic element called a CopyCat element and more traditional genome editing to analyze the control of a gene that coordinates the formation of a simple structure in a fruit fly - a vein in the wing. (elifesciences.org)

Comparison with two other deletions extending beyond COL4A5 in the telomeric direction and generating only ATS allowed us to define the critical region for mental retardation (MR), which contains four candidate genes. (bmj.com)
We narrowed the genetic disease interval and identified a 3,441-bp deletion in the Kcnn2 gene, one of the three candidate genes present in the interval. (pasteur.fr)

Further examination indicated that some had increased copies of the BRCA2 gene and intragenic deletions of varying size that deleted the frameshift mutation and restored expression of the C-terminal portion of the protein. (nature.com)
However, they contributed little to the modern mitochondrial gene pool, probably because of extensive immigration after 1500 A.D.. No evidence of excess mutation was found in the ancient DNA by a Bayesian test, and so there is no reason to suspect that these results be biased by laboratory artefacts in the ancient sequences. (blogspot.com)
HPS-2 is caused by a mutation in the gene encoding the beta-3A subunit of the heterotetrameric AP3 complex ( AP3BA ), which assists in the vesicle formation from the trans-Golgi network or late endosome. (medscape.com)
X-CGD is caused by a mutation in the CYBB gene which encodes a critical component (gp91-phox) of NADPH oxidase, a key enzyme for the anti-microbial activity of phagocytes. (maxcyte.com)
We used a positional cloning strategy to identify the mutation underlying the frissonnant phenotype. (pasteur.fr)
HPS-2 is caused by a mutation in the gene encoding the beta-3A subunit of the heterotetrameric AP3 complex (ADTB3A), which resides on chromosome 5. (medscape.com)
A novel mutation of the CYBB gene resulting in severe form of X-linked chronic granulomatous disease. (lu.se)
A splice-supporting intronic mutation in the last bp position of a cryptic exon within intron 6 of the CYBB gene induces its incorporation into the mRNA causing chronic granulomatous disease (CGD). (lu.se)
Molecular characterization of a novel splice site mutation within the CYBB gene leading to X-linked chronic granulomatous disease. (lu.se)
This disease, previously referred to as MCKD type 1, is due to a mutation in the variable-number tandem repeat region of the MUC1 (Mucin 1) gene. (medscape.com)
Ten of 21 isolates were resistant to penicillin, ciprofloxacin and tetracycline, due mainly to the presence of the bla TEM gene, the S91F mutation in the gyrA gene and the tetM gene, respectively. (who.int)
The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)

SMS is considered a contiguous gene deletion syndrome 1 and 95% of patients harbour a common deletion of approximately 5 Mb. (bmj.com)

Although these clones still expressed the 6174delT mutant protein, they also expressed new BRCA2 proteins that had both the amino and carboxyl termini. (nature.com)
The SMARCAD1 gene provides instructions for making two versions (isoforms) of the SMARCAD1 protein: a full-length isoform and a shorter, skin-specific isoform. (medlineplus.gov)
These genetic changes prevent the production of any functional skin-specific isoform from one copy of the gene, which reduces the total amount of this protein in skin cells. (medlineplus.gov)
The impaired function of specific organelles indicates that the causative genes encode protein complexes that regulate vesicle trafficking in the endolysosomal system including AP-3, BLOC-1, BLOC-2, and BLOC-3. (medscape.com)
Less pronounced severity of immunodeficiency can be due to a novel 2 bp-deletion (c.3222_3223delTG) in the final exon of AP3B1 , causing a frameshift and thus a prolonged altered HPS2 protein. (medscape.com)
The place of the deletion is at the very C-terminal's end, preventing a complete loss of the HPS2 protein. (medscape.com)
In other bacterial pathogens, genes important for pathogenicity and host range include secreted protein effectors that suppress host immunity, alter host metabolism, and enable colonization, providing a fitness advantage. (biorxiv.org)
Results were correlated with immunoreactivity for USP7 (herpes virus-associated ubiquitin-specific protease 7) and SUMO2/3 (small ubiquitin-related modifier protein 2/3) as well as PTEN and p53 FISH gene status. (biomedcentral.com)
This study describes molecular characterization and construction of an infectious clone of the virulent VHSV-Ia strain DK-3592B from Denmark, and application of the clone in reverse genetics to investigate the role of selected VHSV protein(s) in host-specific virulence in rainbow trout (referred to as trout-virulence). (biomedcentral.com)
Using an existing clone of the trout-avirulent VHSV-IVb strain MI03 (pVHSVmi), eight chimeric VHSV clones were constructed in which the coding region(s) of the glycoprotein (G), non-virion protein (NV), G and NV, or G, NV and L (polymerase) genes together, were exchanged between the two clones. (biomedcentral.com)
See also -- positional cloning, protein. (womenshealthsection.com)
DNA sequencing indicated that a genomic deletion of 20-kb in the mutant occurred to the gene model HORVU.MOREX.r3.2HG0202650, which encodes a putative SQUAMOSA promoter-binding protein-like (SPL) transcription factor. (usda.gov)
It is linked to the TBCE gene on chromosome 1q42-43 which encodes for the tubulin-specific chaperone E protein [2-4]. (who.int)

Synthetic genetic array Yeast Deletion Clones: User Manual (PDF). (wikipedia.org)
We report the emergence be in PEDV S gene region in GenBank by BLAST analy- and genetic characterization of a novel PEDV variant with sis (http://blast.ncbi.nlm.nih.gov/Blast.cgi). (cdc.gov)
The full-length isoform is active (expressed) in multiple tissues, where it regulates the activity of a wide variety of genes involved in maintaining the stability of cells' genetic information. (medlineplus.gov)
Five clones of a gene-edited long-tailed macaque with several symptoms of genetic disease have been successfully bred, announced a team of scientists in Shanghai this week. (inverse.com)
Adding on top of that the successful cloning of primates with CRISPR-mediated gene deletions, the researchers have gone to great lengths to study the biological mechanisms for genetic diseases. (inverse.com)
Without the interference of genetic background, a much smaller number of cloned monkeys carrying disease phenotypes may be sufficient for pre-clinical tests of the efficacy of therapeutics. (inverse.com)
Here, we present a suite of tools for genetic manipulation in the tomato pathogen C. michiganensis including a markerless deletion system, an integrative plasmid, and an R package for identification of permissive sites for plasmid integration. (biorxiv.org)
The vector pSelAct-KO is a recombination based, markerless knockout system that uses dual selection to engineer seamless deletions of a region of interest, providing opportunities for repeated higher-order genetic knockouts. (biorxiv.org)
Genetic mapping anchored the Lig 1 gene onto 2H within an '300-kb region. (usda.gov)
We report 8 patients from 7 Jordanian families, 6 of whom underwent genetic testing and were found to have a 12 bp (155-166 del) deletion within the tubulin-specific chaperone E (TBCE gene) in exon 3 at 1q42-43. (who.int)

The original monkey had been altered with CRISPR/Cas9 gene editing technology to give its clones a disrupted circadian rhythm so that scientists can learn how to treat humans with related disorders. (inverse.com)
The patient's own cells are harvested, the mutated gene corrected using CRISPR-mediated gene editing, and the cells returned to the patient. (maxcyte.com)
In these studies, CD34+ hematopoietic stem cells (HSPCs) were isolated from X-CGD patients and electroporated with CRISPR-Cas9, guide RNA and the gene-correcting, oligo template using the MaxCyte GTx. (maxcyte.com)
Using CRISPR technology, we knocked out the SPL-encoding gene and phenocopied the Lig 1 mutant. (usda.gov)

The researchers used somatic cell nuclear transfer, the same technique used to clone Dolly the sheep more than two decades ago, to clone the monkey and produce five cloned offspring. (inverse.com)
Our results indicate that a nuclear factor predisposes to the development of somatic multiple mitochondrial DNA deletions. (nih.gov)

Genomic Deletion, purified, cloned, and sequenced PCR products on an au- tomated DNA sequencer by using T7, SP6 primers, and South Korea newly designed S gene-specific primers (primer sequences available on request). (cdc.gov)

We studied several members of a Swedish family with autosomal dominant progressive external ophthalmoplegia and multiple mitochondrial DNA deletions by polymerase chain reaction analysis of single muscle fibers and by in situ hybridization, combined with enzyme histochemical analysis. (nih.gov)

Virulence of strain B1 was significantly increased by introducing a functional copy of the protease gene on a plasmid. (usda.gov)
To do this, a fragment carrying tra genes and oriT of wild-type F plasmid, which are essential for conjugation and transfer, were combined with oriRg replicon. (cam.ac.uk)
This plasmid can replicate in cells supplying pir gene product. (cam.ac.uk)
The resultant pseudo F plasmid is transferred by conjugation to the newly established single gene deletion mutant and selected Hfr strain integrated F plasmid by antibiotic resistance selection. (cam.ac.uk)

16 Selection for the active X chromosome yielded 12 clones bearing only the normal X and five clones retaining both X chromosomes. (bmj.com)

The human pregnancy-specific glycoprotein genes are tightly linked on the long arm of chromosome 19 and are coordinately expressed. (nih.gov)

The chromosomal fragment of the target site of integration of modified F, is cloned by homologous recombination. (cam.ac.uk)

Bioethicist Carolyn Neuhaus from The Hastings Center told Gizmodo that the research raises a lot of questions, including the fundamental concern that this gene deletion might not actually produce the same effects in humans as it did in the monkeys. (inverse.com)
Physical mapping studies indicate that all patients with the common deletion are haploinsufficient for subunit 3 of the COP9 signalosome ( COPS3 ), which is conserved from plants to humans, and in the plant Arabidopis thaliana regulates gene transcription in response to light. (bmj.com)

Gene deletion, or gene knockout, is one of the main ways in which the function of genes are discovered. (wikipedia.org)

an arrangement which is thought to have evolved by a series of gene duplications. (whispermovie.com)

5] Syrzycka et al[6] demonstrated that the pink gene encodes the Drosophila ortholog of the human HPS5 gene. (medscape.com)

The targeted gene was replaced with a KanMX cassette and tagged with one or two unique 20mer barcode sequences. (biocat.com)
a collection of DNA sequences that code for genes. (womenshealthsection.com)
Amplified HIV DNA was molecularly cloned, and nucleotide sequences of multiple clones were determined. (cdc.gov)

Mitochondrial DNA with multiple different deletions shows clonal expansion, which leads to mitochondrial myopathy with ragged-red fibers and muscle weakness. (nih.gov)
T-cell clonal sizes are probably initially similar across all T-cell clones owing to equal initial intrathymic and peripheral clonal expansion, but become more variable with aging, as homeostatic proliferation of naïve T cells is shaped by selective forces [ 3 ]. (biomedcentral.com)

This deletion is molecularly defined by the identification of a patient specific junction fragment by Southern analysis following pulsed field gel electrophoresis (PFGE). (bmj.com)

Of course, insertions and deletions must also be supported by the setup. (lu.se)

Genomic analysis revealed a 16 amino acid deletion in the protease gene in isolates lacking protease activity, including strain B1. (usda.gov)

This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. (whispermovie.com)
The locus at chromosome 1q21 was identified by linkage mapping in 1998, but the gene has only recently been discovered due to difficulty with sequencing this highly repetitive region and was previously missed using next-generation sequencing. (medscape.com)

To provide a general introduction to the molecular basis of genetics, how genes are maintained from one generation to the next and how their expression is regulated in various systems. (manchester.ac.uk)

We have generated a novel tool for the expression of recombinant proteins in plants and for the functional analysis of virus and plant genes. (biomedcentral.com)
The impaired function of specific organelles indicates that the causative genes encode proteins operative in the formation of lysosomes and vesicles. (medscape.com)

Sequencing of the deletion breakpoints showed that most but not all of the deletions were flanked by direct repeats. (nih.gov)

In this variant, a 16-base pair frame shift duplication occurs at exon 15 of the HPS1 gene. (medscape.com)
This study describes the molecular cloning of the non-coding exon 1 and approximately 2 kb of 5' flanking region of the human PDGF alpha R gene. (nih.gov)

BACKGROUND Smith-Magenis syndrome (SMS) is a multiple congenital anomalies/mental retardation syndrome associated with a hemizygous deletion of chromosome 17, band p11.2. (bmj.com)

The loss of NcACBP resulted in global changes in the expression of multiple genes. (biomedcentral.com)
The fused-toe mutant is a poor model for studying the role of altered Fto activity , because multiple genes are deleted. (gnxp.com)

Interestingly, the remaining 7 resistant clones showed no change in BRAC2 expression and remained sensitive to PARP inhibitors, indicating an alternative mechanism(s) of resistance to cisplatin. (nature.com)
Murcott), We have discovered that the Murcott mandarin Cstps1 gene expression is severely reduced. (biomedcentral.com)
There was a clear relationship between presence or absence of the valencene synthase gene ( Cstps1 ) expression, and presence or absence of valencene among randomly selected mandarin hybrids. (biomedcentral.com)
Cloning and expression of a new pregnancy-specific beta 1-glycoprotein member. (nih.gov)
Pioneering work in the late 1990s through early 2010s resulted in vectors for gene deletion and expression in Clavibacter , but there is still reliance on these tools despite known limitations. (biorxiv.org)
Furthermore, transcriptome analysis was performed to compare the gene expression in different strains. (biomedcentral.com)
The molecular mechanisms involved in the specific regulation of PDGF alpha R expression are unknown, but transcriptional regulation of the PDGF alpha R gene is most likely to be involved. (nih.gov)
This 5' flanking region is a functional promoter of the PDGF alpha R gene as concluded from its capacity to drive luciferase reporter gene expression in an orientation dependent way. (nih.gov)
Histone-modifying enzymes, which regulate eukaryotic chromatin conformation and gene expression, are key epigenetic factors controlling fungal development, virulence, and secondary metabolism. (biomedcentral.com)
Taken together, we demonstrate that Cfhos2 is a key epigenetic factor regulating appressorium development, virulence gene expression, and GLS pathogenesis in C. fructicola . (biomedcentral.com)
Fungal pathogenesis is a sophisticated process including infectious development of the pathogen and fungus-host interactions, which requires a fine-tuned regulation of gene expression dynamics. (biomedcentral.com)
UMOD risk variants identified in the above-mentioned GWAS are located in the promoter region of the gene, leading to a theory that they altered UMOD expression. (medscape.com)
The data presented is normalized to the expression of Rpl32 , a housekeeping gene. (cdc.gov)

RNA-seq based transcriptome analysis highlighted that Cfhos2 regulates secondary metabolism-related virulence genes during infection. (biomedcentral.com)

In progressive external ophthalmoplegia with autosomal dominant inheritance, multiple mitochondrial DNA deletions have been reported. (nih.gov)

Molecular biology (must): Microbial gene deletion and complementation (e.g. (singh-laboratory.com)

Fluorescence in situ hybridisation carried out by both groups indicated that CAPAN1 cells have at least two copies of the mutant 6174delT BRCA2 gene. (nature.com)
These mutant clones formed RAD51 foci when the cells were treated with ionizing radiation, and were also resistant to PARP inhibitors. (nature.com)
FTO is a gene of unknown function in an unknown pathway that was originally cloned as a result of the identification of a fused-toe (Ft) mutant mouse that results from a 1.6-Mb deletion of mouse chromosome 8. (gnxp.com)
In this report, we describe a deletion in the 5′ region of the Kcnn2 gene encoding the SK2 subunit in the mouse neurological frissonnant (fri) mutant. (pasteur.fr)

Transcriptional analysis of ACBP-deficient parasites revealed differentially expressed genes involved in a wide range of biological processes such as lipid metabolism, posttranslational modification, and membrane biogenesis. (biomedcentral.com)

orthogonal exPeriMental changes and pathways induced by EGE investigated the impact of aSSeSSMent of ePigenetic environmental factors in cancer causation, both current H. pylori infection and driver geneS and their link to underpinning studies of etiology e , pigenetic memory of past (eradicated) environMental carcinogenS carcinogen evaluation, and prevention. (who.int)

In 1987 the sdhCAB operon of B. subtilis was cloned and sequenced. (lu.se)

Interestingly this patient did not have the common deletion. (bmj.com)

This highly sensitive phenotypic readout of enhancer function in a native genomic context reveals novel features of CRM function undetected by traditional reporter gene analysis. (elifesciences.org)
A reporter represents (the idea of) a clone, oligo, or other reporter for a gene. (lu.se)
A reporter has a gene name and a gene symbol (short name). (lu.se)

4, 5 Until recently, the only known contiguous gene syndrome involving the COL4A5 gene was Alport syndrome and diffuse leiomyomatosis (ATS-DL, OMIM 308940), 6- 9 in which the deletion extends towards the centromere to include the first two exons of the adjacent COL4A6 gene. (bmj.com)
In 1998, we described a new Xq22.3 contiguous gene syndrome which we named AMME (OMIM 300194) because of the distinctive features observed in affected males: Alport syndrome (A), mental retardation (M), midface hypoplasia (M), and elliptocytosis (E). 10 After the original publication, clinical re-evaluation of the family showed alterations of cardiac rhythm and morphology on echocardiography. (bmj.com)

Back selection for the inactive X on one diploid clone yielded 12 hybrids containing the deleted inactive X chromosome, three of which were selected for analysis. (bmj.com)

The same deletion was found in genomes of isolates from North Dakota and Iran. (usda.gov)
The original monkey was altered as an embryo by knocking out its BMAL1 gene, which is associated with regulating sleep-wake patterns, and the five newborns produced with SCNT all have identical genomes that also lack the BMAL1 gene. (inverse.com)

HPS6 (ruby-eye) have recently been cloned and linked to Hermansky-Pudlak syndrome. (medscape.com)

Cells from patients with deletions in this region, in one case associated with polyposis in a family, have been used to construct human hamster hybrid cell lines that retain either the normal or deleted chromosome 5. (ox.ac.uk)
These lines have been used to identify markers from the region of the polyposis gene obtained by cloning the ends of 0.5- to 2-megabase BssHII fragments purified by pulsed-field gel electrophoresis. (ox.ac.uk)
By further cloning and analyzing the promoter region of Cstps1 from Valencia, Fortune and Murcott, a 12-nucleotide deletion at approximately − 270 bp from the Cstps1 coding region was only found in Murcott. (biomedcentral.com)
The deficiency of valencene production in some mandarins is probably due to a 12-nucleotide deletion in the promoter region of the Cstps1 , which could be a crucial switch of Cstps1 transcription. (biomedcentral.com)
Gene organization of the pregnancy-specific glycoprotein region on human chromosome 19: assembly and analysis of a 700-kb cosmid contig spanning the region. (nih.gov)
a gene located in a chromosome region suspected of being involved in a disease. (womenshealthsection.com)
This effect is mediated through specific transcription factor binding within the -52/+118 region of the PDGF alpha R gene. (nih.gov)

In addition, the morphogen retinoic acid, alone or in combination with dibutyryl cAMP, gives a 22-fold induction of PDGF alpha R gene promoter activity in human teratocarcinoma cells. (nih.gov)

This allows researchers to determine what each gene does by comparing the mutated yeast to the behavior of normal yeast. (wikipedia.org)

Members of the laboratory first cloned red cell ankyrin or Ank1 and subsequently cloned and characterized Ank3, the major nonerythroid ankyrin. (dana-farber.org)

In the paper, they explain that the ability to produce gene-edited clones will help them study diseases related to disrupted circadian rhythm , including Alzheimer's disease, depression, and other sleep problems. (inverse.com)

After 20 weeks the engrafted human cells expressed the corrected gp91 gene at 14% in the mouse peripheral blood and 10% - 20% in the bone marrow. (maxcyte.com)
The phase 1/2 trials showed that CD4 cells with a disrupted CCR5 gene could be engrafted, were safe and persisted, but toxicity related to the adenoviral vector precluded the trials from progressing. (maxcyte.com)

Figure 3: Therapeutic CYBB Gene Correction. (maxcyte.com)
Gene symbol: CYBB. (lu.se)

Together, our result indicate that extracellular protease contributes to virulence of Xanthomonas translucens, but that a subset of environmentally occurring isolates share an in-frame deletion rendering the protease non-functional. (usda.gov)

Compared to current models, procedures with reproducibility plus clone validation before and after infection can benefit other investigators in the field. (jove.com)

When chimeric rVHSVs, containing all the G, NV, and L gene substitutions, were tested in vivo, they did not exhibit any change in trout-virulence relative to the background clones. (biomedcentral.com)
In vivo immunologic selection of proviral gene deletion variants from a nonproducer clone. (wikidata.org)

Exactly one year ago, the same researchers announced that they'd successfully cloned two macaques , named Hua Hua and Zhong Zhong. (inverse.com)
The researchers are undeterred, as the benefits of the cloned monkeys could be significant for drug research. (inverse.com)

Sporadic progressive external ophthalmoplegia and Kearns-Sayre syndrome are usually associated with single large-scale mitochondrial DNA deletions in muscle. (nih.gov)
Muscle fiber segments with deficiency of cytochrome c oxidase, which is partially encoded by mitochondrial DNA, had accumulated mitochondrial DNA with deletions and showed reduced levels of wild-type mitochondrial DNA. (nih.gov)
Young, clinically affected individuals of this family without limb muscle symptoms did not show mitochondrial DNA deletions or cytochrome c oxidase-deficient muscle fibers. (nih.gov)

HPS4 involves the human homolog of the mouse light-ear gene. (medscape.com)

Among the 62 patients without the deletion of ASS who received imatinib treatment, the disease condition of only 12.9% (8 patients) showed progress, suggesting that the deletion of ASS gene could produce drug resistance in the treatment of chronic myelogenous leukaemia. (farmaciajournal.com)
It can be concluded that, the deletion of ASS gene can affect the curative effect of imatinib in treating CML and can promote the progress of the disease. (farmaciajournal.com)

Three genes of unknown function (Fts, Ftm and Fto), along with three members of the Iroquois gene family (Irx3, Irx5, and Irx6 from the IrxB gene cluster), are deleted in Ft mice. (gnxp.com)

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