Cloned populations of antigen-specific, murine T-lymphocytes, maintained in long-term continuous culture, have been reported to exhibit functional activity upon adoptive transfer. (psu.edu)
Few data are currently available on the in vivo distribution of these homogenous, functional cell populations. (psu.edu)
We examined 2 cloned populations of influenza virus-specific T-lymphocytes. (psu.edu)
Using cell populations labeled with [ 3 H]thymidine, we examined the distribution of these cells in tissue sections from various organs. (psu.edu)
These cloned cell populations were preferentially retained in the lungs of uninfected and influenza-virus-infected animals. (psu.edu)
Although class I tetramers identified large populations of virus-specific CD8 + cells, a different picture has emerged from the use of class II tetramers. (aai.org)
Endothelial cells3
We explored whether LPL affects (a) lipoprotein transport across bovine aortic endothelial cells or (b) lipoprotein binding to subendothelial cell matrix (retention). (jci.org)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
Tissue2
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
Gene2
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
The following Slc6a7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
Functional1
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
Terminal1
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Percentage2
In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
Although OspA 165-184 stimulated nearly all OspA-specific human T cell clones tested to proliferate and secrete IFN-γ and IL-13, LFA-1α L326-345 stimulated ∼10% of these clones to proliferate and a greater percentage to secrete IL-13. (aai.org)
Density2
Lipoprotein lipase increases low density lipoprotein retention by subendothelial cell matrix. (jci.org)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Protein4
Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
A marked Ab and T cell response to Bb outer surface protein A (OspA) often develops during prolonged episodes of arthritis. (aai.org)
We have analyzed the Ag-specific CD4 + T cells in treatment-resistant Lyme arthritis, using class II tetramers, and have shown that arthritis-associated Borrelia burgdorferi ( Bb) sensu stricto outer surface protein A (OspA)-specific T cells can be identified with this technology. (aai.org)
Specific8
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
To analyze the nature of response to OspA and LFA-1α L , we used OspA-specific T cell hybrids from DR4 transgenic mice, as well as cloned human cells specific for OspA 165-184 , the immunodominant epitope, from five DRB1*0401 + patients, using OspA-MHC class II tetramers. (aai.org)
Thus, it is of great interest to characterize Ag-specific T cells in individuals afflicted by these illnesses. (aai.org)
Recently, MHC tetramer technology has been developed that allows the direct enumeration of Ag-specific T cells ( 3 , 4 ). (aai.org)
Very low frequencies of collagen/matrix-specific CD4 + cells could be isolated from the PBMC or synovial fluid (SF) 4 of patients with rheumatoid arthritis (RA), using class II tetramers that contained two joint-specific Ags ( 5 ). (aai.org)
In an alternate model, influenza vaccine peptide-bearing tetramers were able to detect influenza-specific T cells only after PBMC from influenza-vaccinated individuals were expanded in vitro by a 7-day culture with Ag ( 6 ). (aai.org)
High2
The mammalian brain high-affinity L-proline transporter is enriched preferentially in synaptic vesicles in a subpopulation of excitatory nerve terminals in rat forebrain. (genscript.com)
Molecular cloning and expression of a high affinity L-proline transporter expressed in putative glutamatergic pathways of rat brain. (genscript.com)
Expression2
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
Development1
12. Scott P. Development and regulation of cell-mediated immunity in experimental leishmaniasis. (ac.ir)
Class2
One clone was a Lyt-2 + , L3T4 - , class I MHC-restricted, cytolytic T-lymphocyte subset. (psu.edu)
The other clone was a Lyt-2 - , L3T4 + , Class II MHC-restricted clone of the helper/amplifier T-cell subset. (psu.edu)
Systems2
The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. (justia.com)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
Genes6
Random integration is the easiest way to obtain transgenic cells, but the integration is unregulated resulting in unwanted genomic disruption and the generation of false-positive cells that incorporate marker genes at incorrect sites. (nature.com)
This sequential expression of specific genes during the transition of cells from a resting to a growing state suggests that there is a genetic program for growth analogous to the developmental program of a complex virus (4-6). (nih.gov)
One way to identify growth factor-respon- sive genes is to search for changes in the expression of known genes encoding suspected regulatory proteins after stimula- tion of resting cells with serum or a purified growth factor. (nih.gov)
In this way the protooncogenes c-fos and c-myc have been found to be activated in a variety of cells (7-10), as have a number of other genes for which probes are available. (nih.gov)
In this communication we concentrate on the expression of 10 genes recently identified by cDNA cloning that are activated almost immediately after resting mouse 3T3 cells are stimulated with serum or a purified growth factor (22). (nih.gov)
Our results thus point to a class of genes, of which c-fos and c-myc are members, that make up the primary genomic response of 3T3 cells to growth factors. (nih.gov)
Presence2
To test this hypothesis, we analysed normal length TNR sequences at a single repeat resolution by radioactive PCR and denaturating polyacrylamide gels, using DNA from multiple single cell clones expanded in the presence or absence of mitomycin-C (MMC), a mutagenic agent able to induce interstrand DNA cross links, strand breaks, and replication blockage. (bmj.com)
Presence of cell death was examined by acridine orange staining during the early or late third instar, between the time of active cellular proliferation in the leg disc and the time of leg eversion. (sdbonline.org)
Mechanisms1
How such factors induce cell growth is a key question, relevant not only to the mechanisms of cell proliferation and neoplasia but more generally to the re- sponse of cells to a variety of extracellular ligands, such as developmental or differentiation factors, classical hormones, or neurotransmitters. (nih.gov)
Mouse1
In this study, we optimized an HDR-mediated knock-in method for mouse embryonic stem cells (mESCs). (nature.com)
Found2
We found vector-containing cells predominantly in the myeloid fraction. (medscape.com)