• Generally porcine oocytes using the 1st polar body at 42 h of maturation (IVM) are utilized for fertilization (IVF) parthenogenetic activation (PA) and somatic cell nuclear transfer research (23 -26) but we discovered that the 1st polar body extrusion price between your oocytes at 33 and 42 h of IVM got no factor. (researchtoactionforum.org)
  • Therefore with this research we likened the proteome signatures of porcine oocytes using the 1st polar body gathered at 33 h (33O) and 42 h (42O) of IVM by MS and 18 differentially indicated proteins between 33O and 42O had been found out. (researchtoactionforum.org)
  • Porcine oocytes using Isoalantolactone the 1st polar body had been acquired at 33 and 42 h for even more experiments. (researchtoactionforum.org)
  • Dynamic status of lysosomal cathepsin in bovine oocytes and preimplantation embryos. (researchmap.jp)
  • oxidative stress is the most prominent one, which will cause damage or alter the genetic material of the sperm and reduce the quality of the oocytes, and has a crucial impact on the possibility of developing embryos even after implantation. (vetmedmosul.com)
  • In conclusion, melatonin could be a promising tool for improving sperm competence for fertilizing oocytes and embryo development in sheep. (vetmedmosul.com)
  • Oocytes, sperms, and embryos are shielded from oxidative stress in their natural environment by free radical scavengers found in oviductal and follicular fluids. (vetmedmosul.com)
  • Oocytes and embryos are not provided with this level of protection when in vitro production (9). (vetmedmosul.com)
  • Melatonin supplementation has been shown to promote bovine and porcine oocytes' maturation rate and increase their blastocyst of IVF embryos (16). (vetmedmosul.com)
  • These results demonstrated that the identification of morphological spindle image of the vitrified-thawed bovine oocytes using Oosight imaging system helps to predict the SCNT embryo quality. (koreascholar.com)
  • For the production of embryos, oocytes recovered from slaughtered excellent grade cow and highly motile frozen‐thawed bull semen (purchased from LIMC, KPN#497) was used. (koreascholar.com)
  • 2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
  • Aberrant methylation of donor genome in cloned bovine embryos. (chinagene.cn)
  • Fine mapping of genome activation in bovine embryos by RNA sequencing. (jimdo.com)
  • 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • The development of embryos to the blastocyst in the diabetic maternal environment will be monitored and embryos will be recovered and processed for structural (multi-color confocal laser scanning microscopy, transmission electron microscopy) and quantitative molecular analyses targeting RNAs, proteins and DNA modifications. (jimdo.com)
  • Among in vitro produced blastocyst embryos at day 8, most healthy embryos were freshly transferred on production day and some frozen embryos were direct transferred on appropriate day. (koreascholar.com)
  • In summary, TSA can significantly improve porcine reconstructed embryo development and transgene expression. (chinagene.cn)
  • In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. (mdpi.com)
  • These results validated a different transcript in pigs and characterized its expression profile in fetal tissues of different gestation stages, which indicated that EZH2 played important roles during porcine embryonic development. (hindawi.com)
  • In the early embryonic development, abnormal expression of EZH2 impaired embryo growth and pluripotency maintenance [ 7 , 8 ]. (hindawi.com)
  • However, the transcript and expression status of the EZH2 gene during porcine embryonic development remains unknown. (hindawi.com)
  • Our study is aimed at providing information for understanding the biological function of EZH2 in porcine embryonic development. (hindawi.com)
  • In order to address the effect of maternal hyperglycemia on preimplantation embryonic development, embryos from wild-type donor animals will be transferred to synchronized diabetic and non-diabetic recipient animals. (jimdo.com)
  • Uncompleted epigenetic reprogramming is attributed to the low efficiency of producing transgenic cloned animals. (chinagene.cn)
  • Production of transgenic cloned piglets from genetically transformed fetal fibroblasts selected by green fluorescent protein. (chinagene.cn)
  • 3] Watanabe S, Iwamoto M, Suzuki SI, Fuchimoto D, Honma D, Nagai T, Hashimoto M, Yazaki S, Sato M, Onishi A. A novel method for the production of transgenic cloned pigs: electroporation-mediated gene transfer to non-cultured cells and subsequent selection with puromycin. (chinagene.cn)
  • Evidence that interferon-tau secreted from Day-7 embryo in vivo generates anti-inflammatory immune response in the bovine uterus. (researchmap.jp)
  • We have also established an ex vivo embryo culture system that allows us to study the function of human cardiac cells in human-mouse or human-pig chimeras. (tum.de)
  • Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious disease and the most economically important disease of the swine industry worldwide. (ijbs.com)
  • PRRSV infection results in severe reproductive failure in gilts and respiratory disease in pigs of all ages and further complicates polymicrobial disease syndromes such as porcine circovirus-associated disease ( 3 ). (ijbs.com)
  • These preparatory interregional and interdisciplinary meetings focused on the following areas: cloning and human reproductive health, biologicals, organ transplantation, research, and medical genetics. (who.int)
  • The main objection to the use of human cloning for reproductive purposes is that it would be contrary to human dignity as it would violate the uniqueness and indeterminateness of the human being. (who.int)
  • Some, however, consider that reproductive cloning could be acceptable in certain cases, such as otherwise untreatable infertility, or to avoid inherited genetic diseases. (who.int)
  • In terms of existing ethical guidelines for biomedical research involving human subjects, human cloning for reproductive purposes raises concerns about risk in relation to benefit, informed consent, and accountability. (who.int)
  • Human cloning for reproductive purposes is seen as having the potential to disrupt intergenerational relations and family structures, with major psychological, social and legal consequences for the individuals and communities concerned. (who.int)
  • Some also perceive reproductive cloning as a high-technology intervention of little relevance to the health needs of the vast majority of the world's population. (who.int)
  • Before the announcement in February 1997 of the cloning of a sheep by somatic cell nuclear transfer, existing legislation in a number of countries already precluded human cloning for reproductive purposes, sometimes implicitly. (who.int)
  • Since then, many countries have adopted government decrees or introduced legislation to impose an explicit ban on human cloning for reproductive purposes. (who.int)
  • However, only a tiny percentage of Assisted reproductive technology (ART)- produced embryos develop fully (1). (vetmedmosul.com)
  • Trans Ova Genetics has been a world-wide leader in bovine ART (assisted reproductive technologies) including embryo transfer and IVF (In Vitro Fertilization) since 1980. (viagenpets.com)
  • To determine the characteristics of the Korean porcine reproductive and respiratory syndrome virus (PRRSV), CA, which was isolated from the serum of an infected pig in 2006, we investigated the nucleotide sequence and expression of the structural ORFs (ORFs 2 to 7) using the bApGOZA system. (koreascholar.com)
  • EXPERIMENTAL Methods Porcine Oocyte IVM Porcine ovaries had been collected from an area slaughter home and held in Isoalantolactone saline at 32-37 °C. Antral follicles (3-5 mm in size) had been aspirated with an 18-measure needle. (researchtoactionforum.org)
  • ROS are free radicals that are continuously produced by oxidative metabolic reactions and increase in vitro conditions, causing aging of both the oocyte (7) and sperm (8), as well as perhaps impairing fertilization and embryo development. (vetmedmosul.com)
  • When a client makes the decision to go forward with cloning, ViaGen takes an unfertilized egg (oocyte) from a donor mare and strips out the DNA. (practicalhorsemanmag.com)
  • Our study established the optimal TSA treatment on porcine donor cells and cloned embryos, 250 nmol/L, 24 h and 40 nmol/L, 24 h, respectively. (chinagene.cn)
  • Furthermore, we found that both the cloned embryo and the donor cell treated by TSA resulted in the highest development efficiency. (chinagene.cn)
  • Meanwhile, TSA can improve transgene expression in donor cell and cloned embryo. (chinagene.cn)
  • Here, we substituted exon 7 of porcine CD163 with the corresponding exon of human CD163-like 1 ( hCD163L1 ) using a CRISPR/Cas9 system combined with a donor vector. (ijbs.com)
  • We chose one bi-allelic knockout cell strain as nuclear donor to produce reconstructed embryos by HMC. (chinagene.cn)
  • In 2007, ViaGen teamed with Trans Ova Genetics to form the joint venture Bovance, to promote bovine cloning in North America. (viagenpets.com)
  • 2] SL, Thomas KR, Capecchi MR. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. (chinagene.cn)
  • We determined nucleotide sequences and inferred experimentally infected with strain SKA-1, which was amino acid sequences of viral protein (VP) 4, VP6, VP7, fi rst isolated from a pig with diarrhea in Tottori Prefecture, and nonstructural protein 4 genes of a porcine rotavirus Japan. (cdc.gov)
  • These pigs are bred, not cloned, meaning the process can be more easily scaled. (ibtimes.com)
  • These pigs are bred rather than cloned, making the process more scalable. (infodailyng.com)
  • Considering the varieties of EZH2 splicing variant, identifying its transcript in porcine fetal tissues is the foundation to study its function. (hindawi.com)
  • Moreover, none of the EZH2 transcript variants have yet been identified in porcine fetal tissues. (hindawi.com)
  • In this study, we identified a transcript variant of EZH2 in porcine fetal tissues by cloning and sequencing. (hindawi.com)
  • Histone modification associated with epigenetics can directly influence the embryo development and transgene expression. (chinagene.cn)
  • TSA improve transgenic porcine cloned embryo development and transgene expression[J]. HEREDITAS, 2011, 33(7): 749-756. (chinagene.cn)
  • As for the IVF medium containing melatonin, it was superior to cysteamine in embryo development rates. (vetmedmosul.com)
  • Scientists attempting to discover the basic requirements for successful embryo development have a hurdle when using in vitro embryo manufacturing strategies (2). (vetmedmosul.com)
  • Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
  • Maternal diabetes is known to adversely affect embryo development and pregnancy outcomes. (jimdo.com)
  • Here we report the generation of site-specific genome modified pig with the newly developed artificially engineered sequence-specific endonucleases (transcription activator-like effector nuclease, TALENs) and handmade cloning (HMC) methods. (chinagene.cn)
  • Trichostatin A (TSA), as an inhibitor of histone deacetylase, can change the status of histone acetylation, improve somatic cell reprogramming, and enhance cloning efficiency. (chinagene.cn)
  • We started in Austin, Texas in January 2002 to provide commercial bovine, equine and porcine genetic preservation and cloning services. (viagenpets.com)
  • Today, all genetic preservation and cloning services for all non-primate mammals is under the umbrella of ViaGen, which is comprised of ViaGen Livestock, ViaGen Equine and ViaGen Pets. (viagenpets.com)
  • Saphir is one of approximately 75 cloned horses who have been produced since the first equine clone?a mule named Idaho Gem?was born in May 2003 in the United States. (practicalhorsemanmag.com)
  • The privately held company was founded in January 2002 to provide commercial bovine, equine and porcine gene banking, cloning and genomics services. (practicalhorsemanmag.com)
  • Molecular cloning and transcriptional mapping of the mouse cellular retinoic acid-binding protein gene. (ulaval.ca)
  • ViaGen is a global expert in animal cloning and genetic preservation, and we are widely recognized as the global leader in these industries. (viagenpets.com)
  • In 2015, ViaGen launched ViaGen Pets specifically to serve pet parents worldwide with our world class genetic preservation and cloning services. (viagenpets.com)
  • ViaGen cloned its first horse in 2006. (practicalhorsemanmag.com)
  • Today, it is responsible for approximately 55 of the cloned horses living in the world, according to Candace Dobson, ViaGen marketing associate. (practicalhorsemanmag.com)
  • The cloning process at ViaGen begins with a $1,500 procedure known as gene banking to gather and preserve the genetic ?information found in an animal's DNA. (practicalhorsemanmag.com)
  • The function from the determined proteins was after that analyzed in cloned embryos and we demonstrate that vimentin (VIM) is necessary for effective nuclear reprogramming in pig. (researchtoactionforum.org)
  • This research Isoalantolactone demonstrates that maternal VIM like a genomic protector is vital for nuclear reprogramming in porcine cloned embryos. (researchtoactionforum.org)
  • detected during a large epidemic of diarrhea in adults in VP6 also showed relatively high identities with those of China in 1997, has been propagated in human embryo group B rotaviruses: 52%-53% at the nucleotide level and kidney cells ( 7 , 8 ). (cdc.gov)
  • This acquisition doubled our cloning capacity, and provided rights to cloning technologies developed by the Roslin Institute of Edinburgh, Scotland - the team who cloned Dolly the sheep. (viagenpets.com)
  • In 2003 it acquired the rights to the cloning technologies developed by the Roslin Institute, the ?research facility in Edinburg, Scotland, where Dolly the sheep was cloned in 1996. (practicalhorsemanmag.com)
  • However, the transcript variant and temporal expression pattern of EZH2 in the middle and late porcine fetus are still unknown. (hindawi.com)
  • Cloning and expression analysis of a LFY homologous gene in Chinese jujube ( Ziziphus jujube Mill. (ajol.info)
  • Grzech M, Dahlhoff M, Herbach N, Habermann FA, Renner-Müller I, Wanke R, Flaswinkel H, Wolf E, Schneider MR. Specific transgene expression in mouse pancreatic beta-cells under the control of the porcine insulin promoter. (jimdo.com)
  • In vitro embryo production is important for research in animal reproduction, embryo transfer, transgenics, and cloning. (umsystem.edu)
  • Production of GHR double-allelic knockout Bama pig by TALENs and handmade cloning[J]. HEREDITAS(Beijing), 2014, 36(9): 903-911. (chinagene.cn)
  • The in vitro embryo production (IVEP) is very stressful for gametes. (vetmedmosul.com)
  • As a result, developing a culture medium capable of effectively reducing ROS in vitro embryo production (IVEP) protocols is of tremendous importance (10). (vetmedmosul.com)
  • First, we constructed the porcine GHR -knockout vector according to TALENs kit protocol. (chinagene.cn)
  • They and cloned into the PCR-TOPO vector by using a TOPO are also responsible for diarrhea in young mammals and TA Cloning Kit (Invitrogen, Carslbad, CA, USA). (cdc.gov)
  • Mares were killed 7.5-8.5 days after transfer and the uterus and oviducts flushed for embryo recovery. (bioone.org)
  • These embryos were produced in a laboratory, embryo transfer (ET) was planned in 10 areas of the remote island (Jeju) from the laboratory by airplane. (koreascholar.com)
  • We collected 173 cell for further positive identification which showed that 46.2% (78/173) of the clones were GHR -knockout cell strains. (chinagene.cn)
  • When the cell death level of embryos according to storage time in thermos (straw container) before ET was measured by TUNEL staining, apoptotic index was increased with storage time‐dependent. (koreascholar.com)
  • Most early research using these compounds focused on their anti-cancer activity, but more recent research has found scriptaid to be useful in other applications such as cloning and research into regulation of metabolism. (wikipedia.org)
  • Precise recapitulation of methylation change in early cloned embryos. (chinagene.cn)