Cloned human cell. Medical search. Frequent questions

The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)
Although attempts have not yet been made to create a therapeutic transplant from embryonic stem cells, the methods have been developed to allow the creation of functional, mature cells using human cell cloning technology. (news-medical.net)
From the ethical point of view, so-called therapeutic cloning is even more serious. (archdiocese-no.org)
It is gravely immoral to sacrifice a human life for therapeutic ends. (archdiocese-no.org)
The heated debate in our society over reproductive cloning, as well as therapeutic cloning to obtain embryonic stem cells, has been fueled by misconceptions and hyperbole on both sides. (flfamily.org)
Two commonly discussed types of human cloning are therapeutic cloning and reproductive cloning. (wikipedia.org)
Therapeutic cloning would involve cloning cells from a human for use in medicine and transplants. (wikipedia.org)
Two common methods of therapeutic cloning that are being researched are somatic-cell nuclear transfer and (more recently) pluripotent stem cell induction. (wikipedia.org)
these are the "holy grail" that would be useful for therapeutic or reproductive cloning. (wikipedia.org)
British scientists have been given permission to perform therapeutic cloning using human embryos for the first time," reported the August 11, 2004, BBC News. (discovery.org)
Is so-called 'therapeutic' human cloning really necessary? (biosafety-info.net)
Human cloning ought to be banned, both reproductive cloning and so-called therapeutic cloning-or as Stanford University recently referred to it, "human nuclear transplantation. (probe.org)
A well-respected medical ethicist from one of Canada's leading universities says Canada must not legalize embryonic cloning for any purpose, including therapeutic purposes. (catholicnewsagency.com)
The reader benefits from the scholar's clear explanation about embryonic stem-cell research and therapeutic cloning. (catholicnewsagency.com)
An Australian ban on therapeutic cloning was lifted in December 2006 after a long debate in Federal parliament. (bioedge.org)
Therapeutic cloning, which creates embryonic stem cells . (medlineplus.gov)
Genome engineering of primary human cells with CRISPR-Cas9 has revolutionized experimental and therapeutic approaches to cell biology, but human myeloid-lineage cells have remained largely genetically intractable. (bvsalud.org)

Reproductive cloning would involve making an entire cloned human, instead of just specific cells or tissues. (wikipedia.org)
Reproductive cloning is expensive and highly inefficient. (wikiquote.org)
Reproductive cloning is performed with the express intent of creating another organism. (echeat.com)
Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)
Elaboration of an international convention against reproductive cloning of human beings has been under consideration in the United Nations since December 2001 when the subject was included in the agenda of the fifty- sixth session as a supplementary agenda item at the request of France and Germany. (who.int)

Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (nih.gov)
When genes of the bacterium and its human host evolve together, the strain is less harmful than that same strain in a person whose ancestors didn't encounter that particular microbe. (sciencenews.org)
A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. (origene.com)
In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons. (origene.com)
But previous approaches required the use of viruses to deliver the four genes needed to activate the cell and accomplish that task. (cbc.ca)
The approach was applied to five different hybridomas producing human monoclonal antibodies and variable regions for both bold gamma and mu heavy chain and kappa and lambda light chain genes were successfully cloned. (lu.se)
Alternative splicing of mRNA generated from both the human and rat PDE4B genes produces long and short splice variants that have unique N-terminal splice regions. (ox.ac.uk)
This clone will facilitate investigations into the role of viral genes on biological properties of HTLV-1 in vitro and in vivo. (wustl.edu)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)

Four embryonic stem cell lines from human fetal somatic cells were derived from those blastocysts. (wikipedia.org)
This report* was developed to assist physicians, public health officials, and other health-care professionals respond to public concerns about recently recognized, serious complications of human parvovirus B19 (B19) infection, including transient aplastic crisis (TAC), chronic anemia, and fetal death. (cdc.gov)
Upon differentiation, other ELLU clones shift from fetal to adult hemoglobin expression, giving rise to more mature cells. (lu.se)
Zika virus (ZIKV) infects fetal neural progenitor cells (NPCs) causing severe neurodevelopmental disorders in utero. (bvsalud.org)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

To clone a human CTR (hCTR), a BIN-67 cDNA library was screened using a cDNA probe from a porcine renal CTR (pCTR) that we recently cloned. (jci.org)
Transfection of this cDNA into COS cells resulted in expression of receptors with high affinity for salmon CT (Kd = approximately 0.44 nM) and for human CT (Kd = approximately 5.4 nM). (jci.org)
The cloned cDNA encodes a putative peptide of 490 amino acids with seven potential transmembrane domains. (jci.org)
cDNA encoding variable regions could be amplified from single hybridoma cells isolated by micromanipulation. (lu.se)
5ʹ-Rapid amplification of cDNA ends, done on poly(A)+ RNA from human U87 cells, was used to identify 420 bp of novel 5ʹ sequence of a PDE4B cAMP-specific phosphodiesterase (PDE). (ox.ac.uk)

By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)
They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
Lee Byeong-chun and Kang Seung-keun have been charged with faking, together with Wang Woo-suk, the results of embryonic stem cell research and of embezzling public funds for the studies. (asianews.it)
In 2011, scientists at the New York Stem Cell Foundation announced that they had succeeded in generating embryonic stem cell lines, but their process involved leaving the oocyte's nucleus in place, resulting in triploid cells, which would not be useful for cloning. (wikipedia.org)
When People Say: "Embryonic stem cell research is. (christianliferesources.com)
And yet, all of us would be appalled at the idea of terminating their lives so we could harvest their tissues or organs in order to save others," she says, in reference to the common utilitarian argument that embryonic stem-cell research is valid in an effort to find cures that could save people's lives. (catholicnewsagency.com)

The Australian government has issued its first license for cloning human embryos to obtain embryonic stem cells. (bioedge.org)

Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)
IL-9 promotes mast cell and T cell proliferation, stimulates mast cell accumulation in tissues, promotes ILC survival, enhances class-switching to IgE in B cells and alters haematopoietic progenitor cell activity. (bioxcell.com)
Embryo-like models with spatially organized morphogenesis and structure of all defining embryonic and extra-embryonic tissues of the post-implantation human conceptus (that is, the embryonic disc, the bilaminar disc, the yolk sac, the chorionic sac and the surrounding trophoblast layer) remain lacking 1 , 2 . (nature.com)
Much of this process relies on the morphogenesis of the extra-embryonic tissues and the effect this has on the organization of embryonic cells. (nature.com)
This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues, such as a patient's own skin cells,' said principal author Andras Nagy, senior investigator at Mount Sinai's Samuel Lunenfeld Research Institute. (cbc.ca)
ABSTRACT The aim of this study was to determine the attitude of Saudi Arabians to research involving storage and use of human tissues from which genetic information may be derived and to assess their willingness to donate tissue samples to biobanks. (who.int)
Researchers hope to use these cells to grow healthy tissue to replace injured or diseased tissues in the human body. (medlineplus.gov)
Our earlier studies using random amplified polymorphic DNA (RAPD) analysis have shown genetic instability in human lung cancer tissues. (cdc.gov)

One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
With that publication, "genome engineering of mammalian cells just took a big step forward," he says. (nationalgeographic.com)
Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)

Human cloning is intrinsically illicit in that, by taking the ethical negativity of techniques of artificial fertilization to their extreme, it seeks to give rise to a new human being without a connection to the act of reciprocal self-giving between the spouses and, more radically, without any link to sexuality. (archdiocese-no.org)
Paul Wagle, M.A., discusses his experience with a life-saving adult stem cell treatment, and the importance of promoting ethical approaches to medical research. (flfamily.org)
These ethical concerns have prompted several nations to pass laws regarding human cloning. (wikipedia.org)
The ability to study human post-implantation development remains limited owing to ethical and technical challenges associated with intrauterine development after implantation 1 . (nature.com)
Scientists, many of whom are sold on utilitarian-based ethical analysis, try to downplay the issue of human life in stem-cell research. (catholicnewsagency.com)
The director of the lobby group Australians for Ethical Stem Cell Research, David van Gend, criticised the issuing of the licence. (bioedge.org)
Even if a clone did survive, the ethical dilemmas of raising a Neanderthal would be complicated. (nationalgeographic.com)

In 2013, scientists reported a successful SCNT procedure by modifying the protocol for specific human oocyte biology. (news-medical.net)
Although the possibility of cloning humans had been the subject of speculation for much of the 20th century, scientists and policymakers began to take the prospect seriously in 1969. (wikipedia.org)
Many nations outlawed it, while a few scientists promised to make a clone within the next few years. (wikipedia.org)
In 2013, a group of scientists led by Shoukhrat Mitalipov published the first report of embryonic stem cells created using SCNT. (wikipedia.org)
Scientists cloned another Jersey calf using the same "standard cell-culturing techniques as compared to the method most commonly u. (echeat.com)
Scientists produced embryonic stem cells from the DNA of one person combined with a human donor egg. (technologyreview.com)
Scientists have been all abuzz in the last few years over stem cells - cellular magicians that promise to dazzle and amaze. (cbc.ca)
Scientists say embryonic stem cells are the most useful type because they have the potential to become any type of cell within the body. (cbc.ca)
Scientists are fascinated by the ability of stem cells to become any type of cell. (cbc.ca)
Chinese scientists have successfully created chimeric embryos containing a combination of human and pig cells. (bioedge.org)
Should scientists seek to clone our ancient hominid cousins? (nationalgeographic.com)
Since the 1996 birth of Dolly the sheep, the world's first cloned mammal, scientists have greatly expanded and improved on cloning techniques. (nationalgeographic.com)
Starting with an intact cell (fresh or frozen) of the animal they'd like to clone, scientists first remove the nucleus, where DNA resides, and insert it into a hollowed-out egg cell of the same or a related species. (nationalgeographic.com)

The term is generally used to refer to artificial human cloning, which is the reproduction of human cells and tissue. (wikipedia.org)
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p16INK4a in human kidney and small intestine tissue sections. (sigmaaldrich.com)
If there are intact cells in this tissue they have been 'stored' frozen. (wikiquote.org)
It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
A blastocyst (cloned or not), because it lacks any trace of a nervous system, has no capacity for suffering or conscious experience in any form - the special properties that, in our view, spell the difference between biological tissue and a human life worthy of respect and rights. (wikiquote.org)
In a study published in the online journal Nature on March 1, 2009, Canadian researches described a new method for generating stem cells from adult human tissue. (cbc.ca)
Cloning describes the processes used to create an exact genetic replica of another cell, tissue or organism. (medlineplus.gov)

This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)

Use Anti-hnRNP L Antibody, clone 4D11 (Mouse Monoclonal Antibody) validated in western blot, immunoprecipitation to detect hnRNP L also known as heterogeneous nuclear ribonucleoprotein L. (fishersci.com)

Pro-cloning forces have been working hard to convince state governments to pass constitutional amendments enshrining a "right" to clone and to destroy embryos for research. (flfamily.org)

In 2004 and 2005, Hwang Woo-suk, a professor at Seoul National University, published two separate articles in the journal Science claiming to have successfully harvested pluripotent, embryonic stem cells from a cloned human blastocyst using SCNT techniques. (wikipedia.org)

a nucleus was taken from a man's leg cell and inserted into a cow's egg from which the nucleus had been removed, and the hybrid cell was cultured and developed into an embryo. (wikipedia.org)
In this case, each embryo was created by taking a nucleus from a skin cell (donated by Wood and a colleague) and inserting it into a human egg from which the nucleus had been removed. (wikipedia.org)
Mouse naive embryonic stem cells have recently been shown to give rise to embryonic and extra-embryonic stem cells capable of self-assembling into post-gastrulation structured stem-cell-based embryo models with spatially organized morphogenesis (called SEMs) 3 . (nature.com)
Implantation of the human embryo leads to a number of changes in organization that are essential for gastrulation and future development 1 . (nature.com)
and (3) evidence of developmental dynamism relating to ability to progress, in a structurally organized manner, through morphologically characterized developmental milestones of the early post-implantation human embryo following initial aggregate formation 3 . (nature.com)
Opponents argue that any embryo has the potential to develop into a mature human. (cbc.ca)
They instead refer to the embryo as a "clump of cells" or as "potential human life," Somerville says, "despite the fact that they are, given the right conditions, human life with the potential to go on living for years and years, just like all of us. (catholicnewsagency.com)

If cloning were to be done for reproduction, this would impose on the resulting individual a predetermined genetic identity, subjecting him - as has been stated - to a form of biological slavery, from which it would be difficult to free himself. (archdiocese-no.org)
Nobel Prize-winning geneticist Joshua Lederberg advocated cloning and genetic engineering in an article in The American Naturalist in 1966 and again, the following year, in The Washington Post. (wikipedia.org)
No persuasive reason exist for cloning save for genetic determinists who believe an organism is nothing more than the sum total of its genetic make-up and that it is their right to exploit cloned human embryos for spare body parts. (biosafety-info.net)
Eve was delivered by Caesarian section from her twin sister (the woman who donated the nuclear genetic material from which she was cloned also served as the surrogate mother). (probe.org)
Using new tricks of genetic engineering, researchers could make adjustments to the DNA in the human cell so it matches the code of the Neanderthal. (nationalgeographic.com)
Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
The copied material, which has the same genetic makeup as the original, is referred to as a clone. (medlineplus.gov)
Here we have investigated the potential for genetic instability in silica- and cadmium chloride (CdCl2)-transformed BALB/c-3T3 cell lines. (cdc.gov)
DNA from non-transformed BALB/c-3T3 cells was used as a control to compare genetic alterations, if any, between non-transformed, transformed and tumor cell populations. (cdc.gov)
This fast, flexible, and scalable platform can be used for genetic studies of human myeloid cells in immune signaling, inflammation, cancer immunology, host-pathogen interactions, and beyond, and could facilitate the development of myeloid cellular therapies. (bvsalud.org)

Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)

Although it is possible to culture structures derived from human blastocysts ex vivo, these cultures do not recapitulate the events and structural organization of the in vivo embryos 6 ( Supplementary Information ). (nature.com)
Characterization of in vivo expanded OspA-specific human T-cell clones. (ox.ac.uk)
Complete sequencing of both alpha and beta chains of a subset of clones showed that at least two distinct T-cell clones were expanded in vivo. (ox.ac.uk)
We have studied five human in vivo activated T cell clones specific for the region 535-551 of human thyroid peroxidase (TPO) established from a Graves' patient. (ox.ac.uk)
Our findings define a route by which human self-reactive T cells may escape thymic selection and become activated in vivo, thus possibly leading to autoimmunity. (ox.ac.uk)
Restricted T cell receptor expression by human T cell clones specific for mycobacterial 65-kDa heat-shock protein: selective in vivo expansion of T cells bearing defined receptors. (ox.ac.uk)
We have examined the T cell receptor (TcR) expression of clones specific for epitopes of mycobacterial 65-kDa heat-shock protein (hsp65) in the context of two different HLA molecules, and used this system as a model to assess the selection of T cells responsive to this antigen in vivo. (ox.ac.uk)
We transfected human and rabbit peripheral blond mononaclear cells (PBMC) with the ACH molecular clone of human T-cell lymphotropic virus type 1 (HTLV-1) to study its in vitro and in vivo properties. (wustl.edu)
Ex vivo manufactured red blood cells (RBC) generated from immortalized erythroid cell lines which can continuously grow are expected to become a significant alternative in future transfusion therapies. (lu.se)

This has led to a lot of interest in SCNT, which is best known as the method used to pioneer whole animal cloning technology, such as Dolly the sheep. (news-medical.net)
With the cloning of a sheep known as Dolly in 1996 by somatic cell nuclear transfer (SCNT), the idea of human cloning became a hot debate topic. (wikipedia.org)
Indeed, if passed, Hatch/Feinstein/Kerry would explicitly legalize doing in humans the very cloning procedure -- somatic cell nuclear transfer (SCNT) -- that was used to make Dolly the sheep . (lifeissues.net)
Comment: Indeed, if passed, "total cloning bans" H.R. 534, H.R. 234, H.R. 916, and S. 245 would not ban anything either - not even the SCNT cloning technique that was used to make Dolly the sheep. (lifeissues.net)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
The most famous clone was a Scottish sheep named Dolly. (medlineplus.gov)

But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
Another successful attempt at human SCNT was made using cells from two adult males. (news-medical.net)
In January 2008, Dr. Andrew French and Samuel Wood of the biotechnology company Stemagen announced that they successfully created the first five mature human embryos using SCNT. (wikipedia.org)
In this experiment, the researchers developed a protocol for using SCNT in human cells, which differs slightly from the one used in other organisms. (wikipedia.org)
Using eggs from adult women who had previously donated for successful fertility treatments, the researchers used SCNT to transfer DNA into the egg cells. (the-scientist.com)

In his speech on "Biological Possibilities for the Human Species of the Next Ten Thousand Years" at the Ciba Foundation Symposium on Man and his Future in 1963, he said: It is extremely hopeful that some human cell lines can be grown on a medium of precisely known chemical composition. (wikipedia.org)
Mycobacterium canettii ," an opportunistic human higher among expatriate than among Djiboutian patients pathogen living in an unknown environmental reservoir, is and that patients with M. canettii infection were significant- the progenitor species from which Mycobacterium tubercu- ly younger than those with M. tuberculosis infection ( 2 ). (cdc.gov)
Given that we have an efficiency of 1% cloning for livestock species and if only one in a thousand cells are viable then around 100,000 cells would need to be transferred. (wikiquote.org)
In 2003, researchers in Spain were the first to bring back an extinct species -the Pyrenean ibex, a wild mountain goat also called a bucardo-though the clone only lived for a few minutes. (nationalgeographic.com)
It starts with a healthy cell of a closely related species-cloning a Neanderthal, for example, could start with a stem cell from a modern human. (nationalgeographic.com)

This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. (origene.com)
The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. (origene.com)
Rarely, mucinous differentiation or squamous cell carcinoma is seen. (medscape.com)
To induce differentiation and maturation of the immortalized cell lines, terminating the HPV-E6/E7 expression through a gene induction system has been believed to be essential. (lu.se)
Here, we report that erythroid cell lines established from human bone marrow using simple expression of HPV-E6/E7 are capable of normal erythroid differentiation, without turning gene expression off. (lu.se)
Our findings propose that it is not necessary to employ gene induction systems to establish immortalized erythroid cell lines sustaining differentiation potential and describe novel cellular characteristics for desired functionally competent clones. (lu.se)
May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. (lu.se)
This process yields genetically edited cells that retain transcript and protein markers of myeloid differentiation and phagocytic function. (bvsalud.org)
Such information is mediated immunity, secreting the cytokine crucial to determine the optimal approach interferon-gamma (IFN) that inhibits Th2 to improve the immune response of DN cell differentiation. (who.int)

Researchers have determined that several steps in the protocol were critical for human cellular reprogramming. (news-medical.net)
Read how other researchers have used the anti-human IL-9 antibody. (bioxcell.com)
Not the fact that the UK will permit researchers to create human cloned embryos-that has been on the drawing board for some time. (discovery.org)
Two other independent researchers, Severino Antinori (an Italian working in an undisclosed Muslim country) and Panos Zavos (from Lexington, Kentucky) have also been hinting at human cloning success and suggesting that a birth will be announced soon. (probe.org)
This incredibly high 50% success rate for human cloning leaves most researchers believing that either this isn't really a clone or they simply aren't revealing all the other failures. (probe.org)
A linkurl:report;http://stemcells.alphamedpress.org/cgi/reprint/2007-0252v1.pdf published online today that researchers have cloned human embryos is not that much of an advance, according to one stem cell expert, Douglas Melton, at Harvard University. (the-scientist.com)
Researchers there are working on technology that induces human skin cells to change into the kind of stem cells that have been created by embryos. (cbc.ca)
However, researchers at the Harvard Stem Cell Institute say reprogrammed cells won't eliminate the need or value of studying embryonic stem cells. (cbc.ca)

Note: Please read The Little Lamb That Made a Monkey of Us All for the author's comments on the news of a successful lamb cloning (March 7, 1997). (probe.org)
General Assembly the following year,3 and the World Medical Association's Resolution on Cloning, endorsed in 1997, have confronted the issue but lack binding legal force. (who.int)

The resulting cells were pluripotent and could be differentiated into insulin-producing beta cells to restore the function of the pancreas in the donor. (news-medical.net)
Embryonic stem cells are pluripotent - they have the ability to become virtually any type of cell within the body. (cbc.ca)

Not only did the HGP achieve its stated goal-sequencing the human genome-it has also had consequences that continue to ripple outward, changing the way we think about biology, changing the way we pursue medicine, and unleashing a host of technological and commercial initiatives. (genengnews.com)
a warning that the kinds of basic research supported by the HGP could be deemphasized in favor of applied research (see Dr. Botstein's November 1, 2012 article in Molecular Biology of the Cell). (genengnews.com)
Today, the human genome, genomics (a rarely used word 15 years ago), and sequencing inform virtually all of biology. (genengnews.com)
In biology , cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria , insects or plants reproduce asexually . (wikiquote.org)

To successfully clone human cells, eggs must be dunked in the stimulant. (sciencenews.org)
He cited the work of two other groups that have also successfully produced human. (the-scientist.com)
The ectopic expression of human papilloma virus (HPV) E6/E7 gene has successfully been employed to establish these cell lines. (lu.se)

Cloning, characterization, and expression of a human calcitonin receptor from an ovarian carcinoma cell line. (jci.org)
Sequencing of the T-cell receptors of the OspA reactive clones showed significant skewing of the T-cell receptor repertoire. (ox.ac.uk)
Human self-reactive T cell clones expressing identical T cell receptor beta chains differ in their ability to recognize a cryptic self-epitope. (ox.ac.uk)
Here we describe an adult stem cell-derived human airway organoid model overexpressing the ACE2 receptor that supports robust viral replication while maintaining 3D architecture and cellular diversity of the airway epithelium. (bvsalud.org)

We are among the first to define the characteristics of TCR usage of T cells isolated from an inflamed immune compartment in an individual with an autoimmune disease potentially triggered by a microbial antigen. (ox.ac.uk)
In eukaryotes, DNA polymerase δ (Pol δ) bound to the proliferating cell nuclear antigen (PCNA) replicates the lagging strand and cooperates with flap endonuclease 1 (FEN1) to process the Okazaki fragments for their ligation. (nature.com)
We have shown that thyroid epithelial cells (TEC), the only cells that produce TPO, express HLA class II molecules in Graves' disease and can act as an antigen-presenting cells, presenting TPO after endogenous processing to autoantigen-reactive T cell clones. (ox.ac.uk)
Sequencing alpha and beta chains of representative V beta 5.2+ and V beta 5.6+ PB-derived clones revealed TcR which were identical to those utilized by the SF-derived clones, showing that the repertoire for recognition of this antigen is stable over time. (ox.ac.uk)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

Complete sequencing and characterization of 21,243 full-length human cDNAs. (nih.gov)

Can Humans Be Cloned Like Sheep? (probe.org)

Going from engineered cells to whole organism has been especially well established in mice, and [there's] no obvious reason why it would fail in other mammals. (nationalgeographic.com)

Western Blotting Analysis: A 1:500 dilution of this antibody detected p16INK4a in 10 µg of HEK293 cell lysate. (sigmaaldrich.com)

In 2010, St. Jude's Research Hospital started the Pediatric Cancer Genome Project, a three-year project to sequence normal and cancer cell genomes of 600 pediatric cancer patients. (genengnews.com)
Developments in biotechnology have raised new concerns about animal welfare, as farm animals now have their genomes modified (genetically engineered) or copied (cloned) to propagate certain traits useful to agribusiness, such as meat yield or feed conversion. (wikiquote.org)
In sexual reproduction, clones are created when a fertilized egg splits to produce identical (monozygous) twins with identical genomes. (who.int)

A panel of CD4 T-cell clones was isolated from synovial fluid by single cell flow cytometry from a patient with treatment-resistant Lyme arthritis using a DRB1*0401 major histocompatibility complex (MHC) class II tetramer covalently loaded with outer surface protein A (OspA) peptide164-175, an immunodominant epitope of Borrelia burgdorferi. (ox.ac.uk)
In addition, T-cell clones were tested functionally for their reactivity to the wild-type peptide as well as to altered peptide ligands (APLs) and peptide libraries based on the OspA epitope in order to determine the TCR contact residues and the stringency in T cell recognition. (ox.ac.uk)
Fine epitope mapping using nested peptides showed that clones using identical TCR beta chains, identical V alpha, but a different J alpha recognized distinct, nonoverlapping epitopes in the TPO 535-551 region. (ox.ac.uk)
Only clone 37 and its sisters recognizes the TPO-EBV cell line, suggesting that the epitope recognized by clone 43 is not presented upon endogenous processing. (ox.ac.uk)
Subtle differences in epitope specificity were detected for two clones with differing TcR. (ox.ac.uk)

The INK and CIP/KIP families of cyclin dependent kinase inhibitors (CDKis) protect cells from oncogenic signals-initiated cellular transformation. (sigmaaldrich.com)
INK4a and INK4b are induced by oncogenic Ras and are involved in cell cycle G1 arrest and cellular senescence. (sigmaaldrich.com)
Cellular cloning refers to a process in which "copies of a cell ar. (echeat.com)
The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. (edu.au)
UPF1 is an RNA-binding protein known to regulate decay of cellular and viral RNAs and is less expressed in ZIKV-infected cells. (bvsalud.org)

He is the co-author of the book The Natural Limits to Biological Change , served as general editor of Creation, Evolution and Modern Science , co-author of Basic Questions on Genetics, Stem Cell Research and Cloning (The BioBasics Series) , and has published numerous journal articles. (probe.org)

Ten years after its "official" completion, what can be said about the Human Genome Project (HGP)? (genengnews.com)
By establishing the order of A's, C's, G's, and T's in the human genome, the HGP essentially set the stage for functional genomics research and clinical translation. (genengnews.com)
GEN asked four questions of the panel: Why should completing the human genome project be considered such a historical accomplishment? (genengnews.com)
What were the main surprises that have come from studying the human genome? (genengnews.com)
What next needs to be done to advance our knowledge and to capitalize on our understanding of the human genome? (genengnews.com)
This work sheds light on the structural basis of Pol δ's activity in replicating the human genome. (nature.com)
Topics covered will include cell membrane structure and function, metabolism, cell motility and division, genome structure and replication, the regulation of gene expression and protein production, genotype to phenotype relationship, and basic principles of inheritance. (middlebury.edu)
In his 2012 book Regenesis , Harvard geneticist George Church proposes a different approach for cloning extinct animals whose genome has been sequenced. (nationalgeographic.com)
That's more difficult than it sounds, as there are millions of spots in the genome that are different in modern humans and Neanderthals. (nationalgeographic.com)

Cloning in biotechnology refers to processes used to create copies of DNA fragments ( molecular cloning ), cells (cell cloning), or organisms . (wikiquote.org)
In transfected COS7 cells the two long forms, HSPDE4B1 and HSPDE4B3, had molecular masses of approx. (ox.ac.uk)
Understanding of the molecular interactions between T cell receptors (TCR) and self-epitopes may explain how T cells escape thymic education and initiate an autoimmune reaction. (ox.ac.uk)
Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)

Human cloning is the creation of a genetically identical copy of a human. (wikipedia.org)
Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
Beyond this scientific interest, the commercial concern in animal cloning focuses on replicating large numbers of genetically identical animals, especially those derived from a progenitor that has been modified genetically. (who.int)

Additionally, IL-9 enhances mucus production from airway epithelial cells and alters barrier function in the intestines. (bioxcell.com)
NF-κB inhibitor alpha was consistently upregulated in infected epithelial cells, and its mRNA expression positively correlated with infection levels. (bvsalud.org)

Perhaps the first step will be the production of a clone from a single fertilized egg, as in Brave New World. (wikipedia.org)

This hybrid egg is then implanted into the uterus of a female surrogate for gestation, and voilà: The surrogate gives birth to a clone. (nationalgeographic.com)
If a human cell could be Neanderthalized, it would be implanted into the womb of a surrogate mother, either a woman or a chimp, and then develop into a fetus. (nationalgeographic.com)

assigns single-cell RNA-seq expression to cancer clones by probabilistically mapping RNA-seq to clone-specific copy number profiles using reparametrization gradient variational inference . (github.com)
Similar studies of TcR expression were carried out on hsp65-specific, DP4-restricted clones derived from the SF of a patient with rheumatoid arthritis by two independent cloning procedures. (ox.ac.uk)
Confocal microscopy showed more IκBα expression in infected than bystander cells, but found concurrent nuclear translocation of NF-κB that IκBα usually prevents. (bvsalud.org)

The first hybrid human clone was created in November 1998, by Advanced Cell Technology. (wikipedia.org)

The cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. (edu.au)
It was concluded that cloning of leukemia and lymphoma cell lines in microwells was the preferred method and that similar techniques could improve the cloning of fresh leukemia and lymphoma cells. (edu.au)
This approach will permit analysis of B cell clonal ontogeny, antibody diversity and lymphoma cell progression and heterogeneity. (lu.se)

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. (origene.com)

DR3-restricted clones were raised from both the synovial fluid (SF) and peripheral blood (PB) of a patient with reactive arthritis in three separate cloning events. (ox.ac.uk)

The researcher claimed he had created stem cell lines from cloned human embryos. (asianews.it)
Hwang claimed to have created eleven different patient-specific stem cell lines. (wikipedia.org)

No objective, even though noble in itself, such as a foreseeable advantage to science, to other human beings or to society, can in any way justify experimentation on living human embryos or foetuses, whether viable or not, either inside or outside the mother's womb. (archdiocese-no.org)
Claims that you could clone individual treatments of human beings to treat common diseases like diabetes, suggests you need a huge supply of human eggs. (wikiquote.org)
more to this argument to quote Scripture that indicates Gods commandments that human beings venerate life. (echeat.com)
By learning more about these topics, you will begin to see how the Church teaching matches up to God's plan and design for human beings. (catholicscomehome.org)

Human hnRNP proteins (from HeLa cells) purified by affinity chromatography on ssDNA agarose. (fishersci.com)

While Somerville does not seem to disagree with the creation of embryos for in vitro fertilization, she states that embryos are indeed human life that should be respected. (catholicnewsagency.com)

Cloning new organs and stem cell research . (globalchange.com)
They are in an early stage of development and have the ability to become any type of cell to form skin, bones, organs or other body parts. (cbc.ca)

The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)

It is unspeakable that we should continue this project of creating living human embryos with the sole purpose of destroying them when the compelling justification for such experiments has gone,' Dr van Gend said. (bioedge.org)

In four pages the sociobiological aspects of cloning are examined in a consideration of Social Darwinism, disease replication, r. (echeat.com)

Common myths about immoral clones and a world full of exact duplicates are refuted in this paper, which argues in favor of continu. (echeat.com)

But there has been intense debate over the use of stem cells. (cbc.ca)

To confirm that clones 37 (and its sisters) and 43 recognize different epitopes, the T cell clones were stimulated with a TPO-transfected autologous Epstein-Barr virus (EBV) cell line (TPO-EBV) that presents TPO epitopes afer endogenous processing. (ox.ac.uk)

A human ovarian small cell carcinoma line (BIN-67) expresses abundant calcitonin (CT) receptors (CTR) (143,000 per cell) that are coupled, to adenylate cyclase. (jci.org)

These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)
These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. (origene.com)
Since then, there has been a flurry of announcements about developments in stem cell research and hints of promising treatments for diseases such as Alzheimer's, Parkinson's and cancer. (cbc.ca)
In May 2007, Ontario and California announced a $30-million stem cell research deal aimed at finding new therapies for those diseases. (cbc.ca)
Produced by the National Institute of Allergy and Infectious Diseases (NIAID), this digitally colorized, scanning electron microscopic (SEM) image depicts four yellow-colored, spheroid shaped, methicillin-resistant, Staphylococcus aureus (MRSA) bacteria, as they were about to be ingested by a much larger, red-colored, human white blood cell (WBC). (cdc.gov)

Anatomy27

Organisms18

Diseases5

Chemicals and Drugs78

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes102

Information Science4

Health Care3

Therapeutic17

Reproductive5

Genes10

Fetal5

CDNA5

Embryonic stem7

Obtain embryonic stem1

Tissues8

Mammalian4

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Scientists13

Tissue7

Somatic cells1

Mouse Monoclonal Antibody1

Embryos for research1

Blastocyst1

Embryo7

Genetic12

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Vivo9

Dolly6

SCNT6

Species5

Differentiation9

Researchers8

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Pluripotent2

Biology4

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Receptor4

Antigen5

Characterization1

Sheep1

Organism1

Dilution1

Genomes3

Epitope5

Cellular5

Genetics1

Genome9

Molecular4

Genetically identical3

Epithelial cells2

Brave New W1

Surrogate2

Expression3

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Lymphoma cell3

Encodes1

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Stem cell lines2

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Epitopes1

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Diseases5