Clone specific genes. Medical search. Frequent questions

Purification of proteins associated with specific genomic Loci. (genscript.com)
and sequence capture which allows targeting of specific regions of the genome encoding immune receptor proteins. (phys.org)
Polycomb Group (PcG) proteins are a family of protein complex that regulate gene expression, especially repressing gene transcription [ 1 ]. (hindawi.com)
Alternative splicing of gene can generate multiple transcripts and proteins to regulate tissue and organ development [ 17 ]. (hindawi.com)
Here, we used a genomics/proteomics approach (including immunoblot experiments from pigs infected with T. suis ) to prioritize putative immunogenic excretory/secretory (E/S) proteins conserved across and specific to several gastrointestinal (GI) parasitic nematode species. (frontiersin.org)
Expression systems are in widespread use for cloning specific genes, for synthesising the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. (nhbs.com)
6) Clone and express degrading proteins for protein purification. (cdc.gov)
Clinical manifestations of PNH occur when a HSC clone carrying somatic PIGA mutations acquires a growth advantage and differentiates, generating mature blood cells that are deficient of GPI-anchored proteins. (medscape.com)

Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
Experiments "show that site-specific mutations in either G or I, but not GT boxes, in the context of the full rbcS-IA promoter, drastically affect the rbcS-IA promoter-dependent expression of Adh and [ β -glucuronidas] GUS reporter genes. (wikiversity.org)
PNH is caused by somatic mutations in PIGA (which encodes phosphatidylinositol N-acetylglucosaminyltransferase subunit A) in one or more HSC clones. (medscape.com)
Collections of these cells (clones) harbouring driver mutations can exist in different tissues without ever transforming into cancer. (who.int)
Preliminary results have revealed clones possessing mutations in the panel of 9 driver genes analysed. (who.int)
Zettner MA, Flor S, Ludewig G, Wagner J, Robertson LW, Lehmann L. Quinoid metabolites of 4-monochlorobiphenyl induce gene mutations in cultured Chinese hamster v79 cells. (who.int)
At least four mutations in the SMARCAD1 gene have been found to cause adermatoglyphia, which is the absence of dermatoglyphs on the hands and feet. (medlineplus.gov)
The mutations that cause adermatoglyphia affect the skin-specific isoform of the SMARCAD1 protein but not the full-length isoform. (medlineplus.gov)
Nousbeck J, Sarig O, Magal L, Warshauer E, Burger B, Itin P, Sprecher E. Mutations in SMARCAD1 cause autosomal dominant adermatoglyphia and perturb the expression of epidermal differentiation-associated genes. (medlineplus.gov)
Loss of expression of the respec- rectal cancers [4,5] and is known to tive mismatch repair genes protein arise due to mutations in DNA mis- Tissue blocks of 32 different patients was defined as absence of nuclear match repair genes [6]. (who.int)

The molecular mechanisms involved in the specific regulation of PDGF alpha R expression are unknown, but transcriptional regulation of the PDGF alpha R gene is most likely to be involved. (nih.gov)
This study describes the molecular cloning of the non-coding exon 1 and approximately 2 kb of 5' flanking region of the human PDGF alpha R gene. (nih.gov)
Molecular cloning and ligand binding studies have shown the alpha 2 class of adrenergic receptor (alpha 2-AR) to be a family of at least three related subtypes in humans. (aspetjournals.org)
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
Organización molecular del complejo II y su rol en la anaerobiosis. (bvsalud.org)
To date, molecular genetic studies of CML BC have mainly focused on alterations of well-known tumor-suppressor genes (e.g. (lu.se)

This gene belongs to the non-CT (non cancer/testis) subgroup of the melanoma-associated antigen (MAGE) superfamily. (nih.gov)
Cloning and subcellular localization of apr-1--a new gene of tumor specific antigen family]. (nih.gov)
More recently, Wilson and others have devised ways to isolate single, antigen-specific B cells from the blood of human donors with active immune responses against a particular pathogen or vaccine. (the-scientist.com)
This technique is best for "when you have an enriched population of antigen-specific cells-they're not there, except for a short time after vaccination or infection," says Wilson. (the-scientist.com)
It would be extremely laborious to use this strategy to try to identify an antibody against an antigen of interest using the blood of a person without an active immune response, he says, because millions of memory B cells are present in the blood, each of which is uniquely specific for a different antigen. (the-scientist.com)
While previous researchers have obtained HIV-neutralizing antibodies using fluorescent probes to pull out antigen-specific memory B cells from a sample of blood, Connors and his team wanted to discover new HIV-neutralizing antibodies, without being limited only to those that are specific for known antigens. (the-scientist.com)
Genotypic pattern observed in small plaque parapsoriasis is similar to that observed in chronic dermatitis, and the pattern of clonality of T cells is consistent with the response of a specific subset of T cells that have been stimulated by an antigen. (medscape.com)
In an attempt to clone some specific parasite antigen genes, a Trypanosoma cruzi cDNA library was constructed in lambda gt11. (bvsalud.org)
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)

To begin this process, we have isolated two mouse genomic clones encoding alpha 2-AR subtypes and expressed these genes in COS-7 cells for binding studies. (aspetjournals.org)
EC 2.1.1.6) was determined from rat cDNA and genomic libraries were screened with DNA probes and specific antiserum. (lu.se)
Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)
Now, in a later version of the genome/GTF file the genomic position may be changed but the actual gene or transcript remains the same. (lu.se)

This showed chromosomes carry genes and described the chromosome recombination. (timetoast.com)
Barbara McClintock found out that genes can jump around on chromosomes. (timetoast.com)
Autosomal" means that the gene in question is located on one of the numbered, or non-sex, chromosomes. (genome.gov)
To address this issue, we used multicolor combined binary ratio fluorescence in situ hybridization (FISH), which allows the simultaneous visualization of all 24 chromosomes in different colors, verified by locus-specific FISH in a series of 33 CML cases. (lu.se)

Insertional inactivation of gtcA brought about loss of reactivity with the serotype 4b-specific monoclonal antibody c74.22 and was accompanied by a complete lack of galactose and a marked reduction in the amounts of glucose on teichoic acid. (asm.org)
A more practical and cost-effective strategy would be to use antibody gene therapy which would provide long term sustainable protection through antibody production within the patient. (frontiersin.org)
By screening single hybrid cells for those that produce the antibody of interest, researchers can develop a cell line generating virtually unlimited amounts of highly specific antibodies. (the-scientist.com)
For each isolated cell, they separately clone the two genes encoding the heavy and light chains that compose an antibody, and that vary in sequence from cell to cell. (the-scientist.com)
Once researchers have cloned the pairs of heavy- and light-chain genes naturally found in the cells, they can produce recombinant versions of each antibody in the lab. (the-scientist.com)
This technique, commonly referred to as expression cloning, "is powerful because you're not limited-any B cell that you can isolate, in any context, you can make an antibody from," Wilson says. (the-scientist.com)
The issue with it is that there are a lot of steps for each cell to clone those antibody genes. (the-scientist.com)
The immortalization of antibody-producing cells from patients infected with HIV can be inefficient, and even when cells are successfully immortalized, some clones tend to be unstable. (the-scientist.com)
But rather than cloning the antibody heavy- and light-chain genes from each cell, as in a typical expression cloning set-up, Connors's group distributed the isolated cells in 384-well plates, where they cultured them in the presence of feeder cells and signaling molecules that help to keep the B cells alive and also stimulate them to secrete antibodies. (the-scientist.com)
Results were compared with those of an immunofluorescence assay (IFA) for FCoV-specific antibody and correlated with health status. (avma.org)
METHODS: We introduce two new methods for diagnosing S. pneumoniae infections by measuring the presence of newly activated, pathogen-specific, circulating Antibody Secreting Cells (ASC). (cdc.gov)
This study as a link to amplify between the pri- significant age-specific increase in in- was therefore conducted to identify mary antibody and the Envision step. (who.int)

The following Orc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)

It enables researchers to search a genetic "library" of resistance genes discovered in wild relatives of modern crops so they can rapidly identify sequences associated with disease fighting capability. (phys.org)
This platform will now be used to genetically map RSA characters in order to clone genes underlying specific RSA genetic traits. (usda.gov)
I have 38 years of experience in the field of plant biotechnology with specific knowledge of genetic engineering, plant tissue culture and nitrogen fixation. (roundtablegroup.com)
We compared their pangenomes, genetic backgrounds (phylogroups, STs, O groups), presence of virulence-associated genes (VAGs) and antimicrobial resistance genes, finding significant differences in all comparisons between commensal and BSI isolates. (bvsalud.org)
The full-length isoform is active (expressed) in multiple tissues, where it regulates the activity of a wide variety of genes involved in maintaining the stability of cells' genetic information. (medlineplus.gov)
These genetic changes prevent the production of any functional skin-specific isoform from one copy of the gene, which reduces the total amount of this protein in skin cells. (medlineplus.gov)
Although it is unclear how these genetic changes cause adermatoglyphia, researchers speculate that a shortage of the skin-specific version of the SMARCAD1 protein impairs signaling pathways needed for normal skin development and function, including the formation of dermatoglyphs. (medlineplus.gov)

Since identification of EZH2 in the research of protooncogene product Vav [ 3 ], studies have shown that EZH2 is highly expressed in tumorigenesis, which regulates the expression of tumor suppressor genes, such as in breast cancer, prostate cancer, and lung cancer [ 4 - 6 ]. (hindawi.com)
Gene ID: 5925, OMIM 614041 ) gene, which encodes a tumor suppressor protein. (molvis.org)
We then transferred the HSV-TK gene to target cells SW1990 using these progeny virions, and treated these gene-modified tumor cells with GCV to study the sensitivity of the cells to GCV and their bystander effects by routine MTT-method. (wjgnet.com)

One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence. (genscript.com)
Sequence homology and ligand binding data allow the assignment of one clone (M alpha 2-4H) as the mouse homolog of the human alpha 2-C4 subtype. (aspetjournals.org)
The other clone (M alpha 2-10H) closely resembles the human alpha 2-C10 subtype in sequence but binds with significantly lower affinity to yohimbine and rauwolscine, members of a distinct class of bulky alpha 2-selective antagonists commonly used to evaluate alpha 2-AR function in vivo. (aspetjournals.org)
Here, we identified the coding sequence of the EZH2 gene and characterized its expression pattern in fetal tissues of Duroc pigs at 65- and 90-day postcoitus (dpc). (hindawi.com)
As in the case of the G box sequence, previous [ribulose-1,5-bisphosphate carboxylase small subunit] RBCS gene expression studies with transgenic plants have failed to demonstrate a requirement for these I box sequences, although mutation of what are likely to be functionally related sequences in CAB genes does affect expression (Gidoni et al. (wikiversity.org)
A protein consists of one or more chains of amino acids (called polypeptides) whose sequence is encoded in a gene. (genome.gov)
Using PCR, researchers then amplify the heavy and light chain genes from antibodies of interest, clone them into expression vectors, and sequence them. (the-scientist.com)
The identity of the clone and the accuracy of the sequence was verified by direct aa sequencing of the tryptic peptides derived from the purified rat liver enzyme. (lu.se)

ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
By using whole-genome sequencing and comparing sequences with those of clones from the United Kingdom, the United States, and Canada, we clarified the traits of the serotype 15A-ST63 clone. (cdc.gov)
By correlating this information with the DNA sequences of the plants, they were able to reveal the identity of functional resistance genes across the population. (phys.org)
However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)
Although the RBCS1, RBCS2 and RBCS3A promoters contain closely related cis regulatory sequences, the expression patterns of the genes are different. (wikiversity.org)

This 5' flanking region is a functional promoter of the PDGF alpha R gene as concluded from its capacity to drive luciferase reporter gene expression in an orientation dependent way. (nih.gov)
We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)

We examined the activities of cloned promoter DNA fragments with luciferase assay. (molvis.org)

Addgene: Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. (addgene.org)

These studies have not, however, identified distinct subtype-specific functions for these receptors in vivo. (aspetjournals.org)
Tests on 49 different olfactory receptors using more than 200 relevant odors eventually led to the identification of the olfactory receptor OR70a as a highly sensitive and specific detector of PAN in the migratory locust Locusta migratoria . (sciencedaily.com)
We are mutating periplasmic receptors normally linked to gene transcription. (igem.org)

In this study, we identified a transcript variant of EZH2 in porcine fetal tissues by cloning and sequencing. (hindawi.com)
We reviewed published microarray and RNA sequencing studies in which gene expression profiles were compared between human RB and normal retina tissues. (molvis.org)
The differences in gene expression profiles induced by the two predators suggest that there are functional differences between the altered tail tissues of the two groups of tadpoles. (biomedcentral.com)
PROMINENT will try to determine how specific risk factors and exposures influence the clonal architecture of normal tissues. (who.int)

Identification of cultivated LAB isolates by phenotypic characterization and 16S rDNA analysis indicated a variety of species, reflecting to a large extent the results obtained from the 16S rDNA clone libraries, with the significant exception of the Lactobacillus delbrueckii species, which dominated in the ripening cheese but was not detected by cultivation. (who.int)
The characterization of ExPECs collected over 10 years showed that 44% of ExPECs were carbapenem-resistant, possessing transmissible blaNDM genes. (bvsalud.org)

ELAV - like1 , methyltransferase like 7A , dolichyl-phosphate mannosyltransferase , laminin subunit beta-1 , gremlin 1 , BCL6 corepressor-like 1 , and three genes of unknown identity. (biomedcentral.com)

The pbp1a gene in meropenem-resistant isolates was identical to that in multidrug (including meropenem)-resistant serotype 19A-ST320 pneumococci, which have spread in the United States. (cdc.gov)
A single nucleotide polymorphism (SNP)-based comparative genome analysis of epidemic clones ST167 and ST131 in a global context revealed that the study isolates were present in close proximity but were distant from global isolates. (bvsalud.org)
The isolates were distributed in around 20 clonal complexes (STC), including two predominant epidemic clones (ST131 and ST167). (bvsalud.org)
P. aeruginosa isolates referred to the ARSP, epidemic clone. (who.int)

However, the transcript and expression status of the EZH2 gene during porcine embryonic development remains unknown. (hindawi.com)
The neuron-specific tau transcript undergoes complex alternative splicing. (nih.gov)

Stanford and UCSF researchers combined a segment of DNA that had a gene from the African clawed frog named Xenopus with DNA from the bacteria E. coli and positioned the result of the DNA back into an E. coli cell. (timetoast.com)
Longitudinal studies of extraintestinal pathogenic Escherichia coli (ExPEC) and epidemic clones of E. coli in association with New Delhi metallo-ß-lactamase (blaNDM) in septicaemic neonates are rare. (bvsalud.org)

However, recent evidence suggest that non-mutagenic promoting stimuli can drive phenotypically normal clones toward malignant transformation. (who.int)

Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star). (bmj.com)
We used a replication defective recombinant retrovirus vector GINaTK (bearing HSV-TK gene) to make packaging cell PA317 produce progeny virions. (wjgnet.com)

You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart. (genscript.com)
Procedures -The 7b gene from a virulent strain of FCoV was cloned into a protein expression vector. (avma.org)

To investigate the effects of loss of β1 function in vivo , we have used gene-targeting methods to produce β1(-/-) mice, and we have analyzed their neuronal phenotypes. (jneurosci.org)
The higher expression of gremlin 1 and HIF1a genes after exposure to dragonfly larvae indicated an in vivo hypoxic reaction, while down-regulation of syndecan-2 may indicate impairment of angiogenesis. (biomedcentral.com)

Complementation of the mutants with the cloned gtcA in trans restored galactose and glucose on teichoic acid to wild-type levels. (asm.org)

What we have now is a library of disease resistance genes and we have developed an algorithm that enables researchers to quickly scan that library and find functional resistance genes," explains Dr. Sanu Arora, first author of the paper. (phys.org)
Functional analyses indicated that some of the genes induced by dragonfly larvae caused an increase in laminins necessary for cell adhesion in the extracellular matrix. (biomedcentral.com)
Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)

Positive clones expressing T pallidum antigens were detected with sera from experimentally infected rabbits. (bmj.com)
First, ASC were detected by ELISpot assays that measure cells secreting antibodies specific for signature antigens. (cdc.gov)

A detailed analysis of the composition of these genes indicated that resistance seems to be caused by pbp1a recombination. (cdc.gov)
Researchers have pioneered a new method which allows them to rapidly recruit disease resistance genes from wild plants and transfer them into domestic crops. (phys.org)
We have found a way to scan the genome of a wild relative of a crop plant and pick out the resistance genes we need: and we can do it in record time. (phys.org)
The research published today in the journal Nature Biotechnology reveals that AgRenSeq has been successfully trialled in a wild relative of wheat-with researchers identifying and cloning four resistance genes for the devastating stem rust pathogen in the space of months. (phys.org)
Re-introducing disease resistance genes from wild relatives is an economic and environmentally sustainable approach to breeding more resilient crops. (phys.org)
Our results demonstrate that AgRenSeq is a robust protocol for rapidly discovering resistance genes from a genetically diverse panel of a wild crop relative," he says. (phys.org)
[ 10 ] Multiple biofilm-specific mechanisms are operated simultaneously in a reversible and transient manner contributing to the high levels of antibiotic resistance of biofilms, and these are distinct from the well-characterized intrinsic resistance mechanisms (e.g., expression of antibiotic-degrading enzymes, inducible decrease in antibiotic influx, inducible increase in antibiotic efflux and alteration in antibiotic target sites) employed by planktonic cells. (medscape.com)
In addition, long-read sequencing of one representative XDR ST235 isolate identified an integron carrying multiple resistance genes (including bla VIM-2), with differences in gene composition and synteny from the P. aeruginosa class 1 integrons described previously. (who.int)

This problem would be solved by having the positions as annotations to the array design features instead, while other gene-centric annotations can remain on the reporter. (lu.se)
The clone reporter functionality ( #1616 ) should be able to also clone feature annotations. (lu.se)

The restriction analysis and partial sequencing of the gene shows that it contains (1) four alternatively spliced exons previously described in rodent and bovine but not in human tau cDNAs and (2) two CpG islands, one associated with the promoter region, the other with exon 9. (nih.gov)
Examination of human tau mRNA indicates that the human cerebrocortical splicing pattern differs from that previously reported for the murine and bovine tau mRNAs, despite conserved exon organization in all three genes. (nih.gov)

Employing the DNA blot analysis only one COMT-encoding gene was found in the rat genome. (lu.se)
Southern blot analysis of T-cell receptor genes from parapsoriasis does not identify a dominant clone of T cells. (medscape.com)

Histone H2B type 1-A is a protein that in humans is encoded by the HIST1H2BA gene. (wikipedia.org)
ADH is a dimeric zinc metalloprotein and occurs as five different classes in humans, resulting from gene duplications during vertebrate evolution, the first one traced to ~500. (researchgate.net)

In addition, the morphogen retinoic acid, alone or in combination with dibutyryl cAMP, gives a 22-fold induction of PDGF alpha R gene promoter activity in human teratocarcinoma cells. (nih.gov)
Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. (addgene.org)
Whereas the RBCS1 and RBCS2 genes are expressed in both leaves and young fruit, the RBCS3A promoter is highly active in leaves, but not in young fruit. (wikiversity.org)
The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
We have cloned this population sensor, however, and placed its promoter over a DNA gyrase poison, cuing its "suicide" when populations have reached a dangerous level. (igem.org)

We found evidence of persistence or reintroduction of the predominant clone ST235 in one hospital, and of transfer between hospitals. (who.int)

And, more significantly, the progress achieved recently in human genomics research,1 the creation of genetically-modified foods2 and the gene therapy3 have added to the major bioethical issues the Region is likely to face in the near future. (who.int)

The presence of antibiotic-resistant epidemic clones causing sepsis calls for a modification of the recommended antibiotics with which to treat neonatal sepsis. (bvsalud.org)

Recombination site prediction analysis showed that the meropenem-resistant strain-specific recombination regions included the pbp1a and pbp2b regions. (cdc.gov)

assay as gene expression input. (github.com)

The technique called AgRenSeq or speed cloning has been developed by John Innes Centre researchers alongside colleagues in the United States and Australia to speed up the fight against pathogens that threaten food crops worldwide. (phys.org)
From there researchers can use laboratory techniques to clone the genes and introduce them into elite varieties of domestic crops to protect them against pathogens and pests such as rusts, powdery mildew and Hessian fly. (phys.org)
El-Darouti et al reported on a 7-year study of a hypopigmented disorder that the researchers believe should be classified as a new variant of parapsoriasis en plaque. (medscape.com)

I have published on such topics as gene isolation, cloning, plant transformation and protein purification. (roundtablegroup.com)

new nomenclature, gene organization, and divergence of human and rodent genes. (genscript.com)
Human testis/sperm-specific histone H2B (hTSH2B). (wikipedia.org)
The human tau gene has been cloned and mapped. (nih.gov)
A comprehensive understanding of gene expression in human RB is essential for the development of safe and effective new therapies. (molvis.org)
To investigate the in vitro effects of suicide gene therapy system of herpes simplex virus thymidine kinase gene (HSV-TK) in combination with the treatment of nucleotide analog-ganciclovir (GCV) on human pancreatic cancer, and to provide a novel clinical therapeutic method for human pancreatic cancer. (wjgnet.com)
Our data demonstrate that HSV-TK/GCV suicide gene therapy system is effective for treating experimental human pancreatic cancer, which is largely resistant to the common therapies, so the suicide gene therapy system may be a potential treatment approach for pancreatic cancer. (wjgnet.com)

This effect is mediated through specific transcription factor binding within the -52/+118 region of the PDGF alpha R gene. (nih.gov)
We investigated the expression of genes of interest using quantitative reverse transcription PCR. (molvis.org)

In tomato, the RBCS gene family consists of five members at three chromosomal loci ( RBCS1 , RBCS2 , RBCS3A , RBCS3B and RBCS3C ). (wikiversity.org)
Balanced secondary changes are rare in CML AP/BC, but it is not known whether cryptic chromosomal translocations, generating fusion genes, may be responsible for disease progression in a subgroup of CML. (lu.se)

Ezh2 orchestrates gene expression for the stepwise differentiation of tissue-specific stem cells. (genscript.com)
We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
B ) Ancestor cells expressing cerulean fluorescent protein (CFP + ) were mixed with the populations E6, E15 and the clone E6-1 (none of which expressed CFP, (CFP − )), sonicated to single cells, and imaged. (elifesciences.org)
The skin-specific isoform is expressed only in skin cells, and little is known about its function. (medlineplus.gov)

In serotype 4b, gtcA appears to be the first member of a bicistronic operon which includes a gene with homology to Bacillus subtilis rpmE , encoding ribosomal protein L31. (asm.org)

This gene is intronless and encodes a testis/sperm-specific member of the histone H2B family. (wikipedia.org)

Gene probes can be used to locate specific segments of normal or mutated DNA. (msdmanuals.com)
Gene probes can detect a number of disorders before and after birth. (msdmanuals.com)

GXD's primary emphasis is on endogenous gene expression during development. (jax.org)

Bacterial and Lactobacillus group-specific primers were used to amplify the V6 to V8 and V1 to V3 regions of the 16S rRNA gene, respectively. (who.int)

Furthermore, the decreased expression of the EZH2 gene is crucial for stem cell differentiation into specific cell lineages involved in myogenesis, adipogenesis, osteogenesis, neurogenesis, and haematopoiesis [ 9 ]. (hindawi.com)
Thus, the appropriate expression level of the EZH2 gene is important for embryonic development. (hindawi.com)

Sample Population -95 serum samples submitted for various diagnostic assays and 20 samples from specific-pathogen-free cats tested as negative control samples. (avma.org)

Conclusion: Expression of ERh is an independent marker for favorable prognosis after adjuvant tamoxifen treatment in ERa-negative breast cancer patients and involves a gene expression program distinct from ERa. (lu.se)
CONCLUSION: Specific antibodies produced by activated ASC may provide a simple diagnostic for ongoing S. pneumoniae infections. (cdc.gov)

When Barbara figured out that genes can "jump" she than correlated microscopic arrangments of chromosome placements. (timetoast.com)

Having speed cloning in our toolkit means that elite crops can be made more resilient which means higher yields and reduced reliance on pesticides to protect crops," he says. (phys.org)

As one of the two distinct complexes, namely, Polycomb Repressive Complex 1 (PRC1) and PRC2, PRC2 mediates gene silencing by modulating chromatin structure [ 2 ]. (hindawi.com)

Objective -To determine whether expression of feline coronavirus (FCoV) 7b protein, as indicated by the presence of specific serum antibodies, consistently correlated with occurrence of feline infectious peritonitis (FIP) in cats. (avma.org)
Some healthy cats with detectable FCoV-specific antibodies as determined via IFA were seronegative for antibodies against the 7b protein. (avma.org)
Geometric mean concentrations (GMC) of pertussis-specific IgG antibodies (IU/mL) in cord blood of infants born to women born after 1997 (aP-primed) were compared with those born to women born before 1992 (wP-primed). (cdc.gov)

80%) and biological pathways (such as adipocytokine, Notch, Hedgehog and NOD-like receptor signaling) were not identified by previous gene array studies. (mdpi.com)

Cloning and biological comparison of Restin, novel member of Mage superfamily. (nih.gov)

A conditional knockout resource for the genome-wide study of mouse gene function. (genscript.com)

Anatomy23

Organisms20

Diseases5

Chemicals and Drugs55

Analytical, Diagnostic and Therapeutic Techniques and Equipment41

Psychiatry and Psychology1

Phenomena and Processes113

Disciplines and Occupations5

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science7

Health Care3

Proteins8

Mutations10

Molecular6

Antigen9

Genomic4

Chromosomes4

Antibody12

CDNA ORF clone1

Genetic7

Tumor3

Sequence8

Sequences5

Luciferase reporter gene2

Fragments1

Target genes1

Receptors3

Tissues4

Characterization2

Subunit1

Isolates4

Transcript2

Coli2

Malignant transformation1

Recombinant2

Vector2

Vivo2

Mutants1

Functional3

Antigens2

Resistance8

Reporter2

Exon2

Blot analysis2

Humans2

Promoter5

Predominant1

Genetically1

Epidemic1

Recombination1

Assay1

Researchers3

Protein purification1

Human6

Transcription2

Chromosomal2

Cells4

Homology1

Encodes1

Probes2

Endogenous1

Amplify1

Ezh22

Pathogen1

CONCLUSION2

Chromosome1

ToolKit1

Chromatin1

Antibodies3

Pathways1

Biological1

Genome-wide1