• Anti-SOCS1 Antibody, clone 4H1 is an antibody against SOCS1 for use in immunoprecipitation and western blotting. (fishersci.com)
  • TNFRII antibody was raised in Mouse using recombinant soluble human TNFRII-Fc fusion protein as the immunogen. (antibodies-online.com)
  • The following product was used in this experiment: SOX9 Recombinant Rabbit Monoclonal Antibody (5Y4J8) from Thermo Fisher Scientific, catalog # MA5-42728, RRID AB_2911869. (thermofisher.com)
  • The present study was undertaken to determine the antitumor effects of an antibody-conjugate against fibroblast activation protein-α, a cell surface protease of activated tumor fibroblasts. (aacrjournals.org)
  • A novel antibody-maytansinoid conjugate, monoclonal antibody (mAb) FAP5-DM1, was developed to target a shared epitope of human, mouse, and cynomolgus monkey fibroblast activation protein-α, enabling preclinical efficacy and tolerability assessments. (aacrjournals.org)
  • This antibody has been identified in several sources (bacteria, yeast, and mammalian cells) of cMyc fusion protein. (leadgenebio.com)
  • The clone number has been updated from S57-46 to L57/46, both clone numbers name the same antibody clone. (abcam.com)
  • One hybridoma, producing the monoclonal antibody H7, against placental alkaline phosphatase was adopted to offer CDR-containing regions for new recombinant constructs. (projectsparadise.com)
  • We show that the S1-Fc fusion protein is extraordinarily immunogenic, as evidenced by robust antibody titers noticed by day 7. (genoimmun.com)
  • One strategy for enhancing the serum half-life of proteins includes increasing the molecular weight of the protein of interest by fusion to the Fe part of an antibody. (boku.ac.at)
  • By using these techniques for the characterization of the recombinant human Epo-Fc (rhEpo-Fc) molecule itself and furthermore, for the separate characterization of both subunits, we could clearly show that no significant differences in the core glycan structures compared to rhEpo and human antibody N-glycans were found. (boku.ac.at)
  • This antibody is specific for Myc tagged proteins. (abcam.com)
  • 30 µg of 293 cell lysate per lane was probed with 3 µg/ml (lane 1) or 1 µg/ml (lane 2) of anti- MLH1 antibody (clone G168-728). (bdbiosciences.com)
  • Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. (bdbiosciences.com)
  • To minimize the problems due to use of pure mouse antibody, researchers have used recombinant DNA techniques to create monoclonal antibodies that are part human and part mouse. (msdmanuals.com)
  • C57BL/6 mouse splenocytes were stained with purified anti-mouse CD127 (IL-7Rα) recombinant (clone QA17A28) (left) or purified mouse IgG1a, κ isotype control (right) then followed by rat anti-mouse IgG1 PE. (biolegend.com)
  • Recombinant fusion protein containing a sequence corresponding to amino acids 169-300 of human SOX9 (P48436). (thermofisher.com)
  • Sequence the DNA: After final step of selection and electrophoresis, clone the DNA into a cloning vector and sequence it. (wikipedia.org)
  • We can apply the technique to less affinity binding sequence also, provided to keep high concentration of binding protein at each step of binding. (wikipedia.org)
  • It is also possible to identify the binding site even if both the protein and sequence is not known. (wikipedia.org)
  • InvivoGen offers a collection of pFUSE-Fc plasmids developed to facilitate the expression and production of Fc-Fusion proteins, by fusing a sequence encoding a given protein to the Fc region of an immunoglobulin (Ig). (invivogen.com)
  • Vectors containing the preprotrypsin leader (PPT) sequence direct secretion of FLAG fusion proteins into the culture medium for purification using ANTI-FLAG antibodies, resins, and plates. (sigmaaldrich.com)
  • A recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human FAP. (thermofisher.cn)
  • Morphological transformation studies have failed to demonstrate a viral oncogene, a virus-coded transforming protein or any sequence of DNA that uniquely transforms cells according to one-hit kinetics. (microbiologyresearch.org)
  • IGF II was released from the fusion protein produced by BM-N cells infected with the recombinant virus by CNBr treatment, purified by extraction with guanidine-HCl, column chromatography and HPLC and the correct amino-terminal amino acid sequence confirmed. (microbiologyresearch.org)
  • Purification and initial analysis of a His-tagged protein from Escherichia coli 4. (medicpdf.com)
  • Conversion of this insoluble material involves often slow and complex refolding methods, making protein purification difficult. (justia.com)
  • However, overproduction of recombinant proteins in mammalian cells is well established, analysis of product quality of complex products for different purposes, such as product specification, purification issues, batch to batch consistency and therapeutical consequences, is required. (boku.ac.at)
  • Tissue culture supernatant is purified by either protein A/G or affinity purification methods. (bdbiosciences.com)
  • Selection and amplification binding assay (SAAB) is a molecular biology technique typically used to find the DNA binding site for proteins. (wikipedia.org)
  • Description: CDC37 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 378 amino acids and having a molecular mass of 44.4 kDa. (genoimmun.com)
  • Then pJHL65-M2eCD40L or pcDNA-M2eCD40L recombinant plasmid was electroporated into JOL967 strain and the resultant clones were designated as JOL2074 and JOL2076, respectively. (biomedcentral.com)
  • This fusion protein gene was combined in a plasmid with the promoter and 5′ and 3′ flanking regions of the polyhedrin gene. (microbiologyresearch.org)
  • The resulting plasmid and the wild-type BmNPV genomic DNA were cotransfected into BM-N cells, and a recombinant virus was isolated by the limiting dilution method. (microbiologyresearch.org)
  • mouse SOCS1 recombinant GST fusion protein encompassing amino acids 2-78. (fishersci.com)
  • production of perdueterated proteins for neutron-scattering experiments. (lu.se)
  • The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. (caslab.com)
  • Production of the fusion polypeptide involves the introduction of the hybrid gene into a biological host cell system, for example yeast cells, which permits the expression and accumulation of the fusion polypeptide. (justia.com)
  • Positional cloning of the RID2 gene revealed that it encodes an evolutionarily conserved methyltransferase-like protein, which was found to localize in the nucleus, with accumulation being most evident in the nucleolus. (nih.gov)
  • The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. (thermofisher.cn)
  • A gene coding for insulin-like growth factor II (IGF II) was constructed from 16 oligodeoxynucleotides synthesized chemically and cloned into Eco RI- Sal I sites of pBR322. (microbiologyresearch.org)
  • The coding region of the carboxy- terminal half of polyhedrin was removed and the remainder was ligated with the IGF II gene in phase to create a fusion protein gene consisting of the coding region of the amino-terminal half of polyhedrin and the IGF II gene. (microbiologyresearch.org)
  • Additionally, recombinant SARS-CoV-2 Fc-fusion proteins are potential COVID-19 vaccine candidates. (invivogen.com)
  • On this research, we examined the immunogenicity of CHO-expressed recombinant SARS-CoV-2 S1-Fc fusion protein in mice, rabbits, and monkeys as a possible candidate for a COVID-19 vaccine . (genoimmun.com)
  • Inhibition of sperm-oocyte binding and sperm-oocyte fusion make fertilin an attractive target for the development of an immunocontraceptive vaccine. (edu.au)
  • Our selection of CMV vectors provides options for transient or stable expression of 3xFLAG ® fusion proteins. (sigmaaldrich.com)
  • Design of cassette vectors permitting cloning of all types of human TCR variable alpha and beta regions. (ox.ac.uk)
  • Using these cassette vectors, we reconstituted the original HLA-restricted antigen specificity for two human T cell clones, one recognizing an immunodominant epitope of HIV-1 gp120, and the other recognizing an immunodominant epitope of HIV-1 reverse transcriptase. (ox.ac.uk)
  • The amplified Quox1 DNA fragment obtained from PCR amplification from a human embryo cDNA librarywas digested with EcoRV and XhoI and cloned into the SmaI and XhoI restriction site of the expression vector pGEMEXxBal. (wikipedia.org)
  • The strong human cytomegalovirus (CMV) promoter regulatory region drives constitutive protein expression levels as high as 1 mG/L in COS cells. (sigmaaldrich.com)
  • Antibodies to ribosomal P2 protein were rarely detected, ransmission routes of human parvovirus 4 (PARV4), except in patients with systemic lupus erythematosus ( 5 ). (cdc.gov)
  • No. 4,743,679 which discloses a process for the production of human epidermal growth factor comprising cleavage of a fusion protein by Staphylococcus aureus V8 protease. (justia.com)
  • T cell clones are an irreplaceable asset for the study of immune responses relevant to human pathologies. (ox.ac.uk)
  • In order to reconstitute functional human T cell receptors (TCRs) into stable and fast growing hybridoma T cells, we developed a general approach based on a versatile cassette system, which allows cloning of all types of human T cell receptor variable alpha and beta region genes fused to murine constant regions. (ox.ac.uk)
  • Ectodomain of recombinant human 4-1BB fusion protein. (adipogen.com)
  • In this context, we have expressed a homodimer fusion protein in CHO cells which consists of two identical polypeptide chains, in which our target protein, recombinant human erythropoietin (rhEpo), is N-terminally linked with the Fc part of a human IgG(1) molecule. (boku.ac.at)
  • We emphasized product quality aspects regarding the fusion protein itself and in addition, post-translational characterization of the subunits in comparison to human antibodies and rhEpo. (boku.ac.at)
  • The most persuasive roles for HSV and HCMV in human tumourigenesis are as mutagens, as activators of cellular transcription or in switching on the synthesis of host cell proteins not normally expressed in untransformed cells. (microbiologyresearch.org)
  • Full-length human recombinant MLH was expressed as a maltose binding-MLH fusion protein, affinity purified, and used as immunogen. (bdbiosciences.com)
  • Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)
  • The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. (caslab.com)
  • Epitope binning studies on the Carterra LSA revealed that hybridoma antibodies covered more diverse epitope regions but potency in functional assays was weaker across all bins while B Cell Cloning antibodies were concentrated in fewer epitope bins that represented greater potency. (chempartner.com)
  • We found that the reconstituted hybridomas maintain the ability of the original T cell clones to recognize the appropriate epitope in the context of the relevant MHC either as a synthetic peptide or after processing. (ox.ac.uk)
  • Jensen-Shannon and Grantham scores were computed for mutations in protein-coding genes to evaluate whether they affected protein function. (cdc.gov)
  • KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM) , while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. (caslab.com)
  • ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. (thermofisher.com)
  • The gG polypeptides were present on cytoplasmic and nuclear membranes during infection with both HSV-1 and recombinant vaccinia virus gG- VAC. (microbiologyresearch.org)
  • 2006): Biochemical characterization of rhEpo-Fc fusion protein expressed in CHO cells. (boku.ac.at)
  • In the present study, culture supernatant of a stable clone was collected and purified by affinity chromatography prior characterization. (boku.ac.at)
  • Dipeptidyl peptidase (DPP) 9, DPP8, DPP4 and fibroblast activation protein (FAP) are the four enzymatically active members of the S9b protease family. (preprints.org)
  • Recombinant HIPKs and DYRK1A are auto-activated and phosphorylate the negative elongation factor SPT5, the transcription factor c-Myc, and the C-terminal domain of RNA polymerase II, suggesting a direct function in transcriptional regulation. (nature.com)
  • Furthermore, MAP3K16 recombinant protein could phosphorylate MKK3 and ABR1, suggesting that they might be MAP3K16 substrates. (molcells.org)
  • Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
  • Hyperimmune mouse serum raised against purified, recombinant OmpP4 did not promote bactericidal killing of 35000HP or phagocytosis by J774A.1 mouse macrophages in serum bactericidal and phagocytosis assays, respectively. (biomedcentral.com)
  • Anti-EDN3, mouse monoclonal, clone 2A6-2A4, recognizes the ~26 kDa EDN3 protein in LNCaP cells. (emdmillipore.com)
  • In yeast the proteins are called MutS homolog 2 (MSH2), MutL homolog (MLH1), and PMS1 which is also a homolog of MutL. (bdbiosciences.com)
  • clone G168-15 may also be stronger for western blot analysis than clone G168-728 in some assay systems. (bdbiosciences.com)
  • Description: Candida Albicans (strain SC5314 / ATCC MYA-2876) Hsp90 co-chaperone Cdc37, recombinant protein. (genoimmun.com)
  • BmNPV genomic DNA codes polyhedrin which is a major protein of inclusion bodies and is mass- produced in infected silkworm larvae. (microbiologyresearch.org)
  • The silkworm larvae infected with the recombinant virus produced 3·6 mg of the fusion protein per larva and the infected BM-N cells produced 0·3 mg per ml of culture. (microbiologyresearch.org)
  • The course will be held in collaboration with the Lund Protein Production Platform, LP3 ( http://www.lu.se/lp3 ). (lu.se)
  • In the Arabidopsis genome, approximately 80 MAP3Ks (mitogen-activated protein kinase kinase kinases) have been identified. (molcells.org)
  • Fusion protein corresponding to Rabbit CACNA1C aa 1500-1750 (C terminal). (abcam.com)
  • Detection of fusion proteins containing 3xFLAG ® is enhanced up to 200 times more than any other system. (sigmaaldrich.com)
  • In this chapter, we describe the use of two stable STED dyes in combination with expansion microscopy, which allows the robust detection by conventional and STED microscopy of proteins immunolabeled prior to sample expansion. (bvsalud.org)
  • Secondly, those proteins which are present in soluble form in the cytoplasm often are subject to degradation by host specific enzymes, thus reducing the amounts of active protein that can be recovered. (justia.com)
  • If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)
  • Bone morphogenetic proteins (BMPs) are osteoinductive substances and have been used in bone regeneration. (bvsalud.org)
  • Fusion partners known to the prior art include maltose binding protein (Di Guan et al. (justia.com)
  • Fc-fusion proteins are molecules consisting of an IgG-Fc domain fused to a protein of interest, such as an extracellular domain of a receptor, ligand, enzyme, or peptide. (invivogen.com)
  • Homeodomain-interacting protein kinases (HIPKs) are an evolutionarily conserved kinase family comprising the members HIPK1, HIPK2, and HIPK3 in vertebrates, and, additionally, HIPK4 in mammals. (nature.com)
  • Finally, the use of a fusion partner may enable the production of a peptide which would otherwise be too small to accumulate and recover efficiently from a recombinant host cell system. (justia.com)
  • The preparation of valuable recombinant (genetically engineered) polypeptides, for example pharmaceutical proteins, relies frequently on techniques which involve the production of these polypeptides as fusion or hybrid proteins. (justia.com)
  • The search for materials presenting characteristics similar to those achieved with autograft, aiming to reduce morbidity in procedures of restoration of lost bone structures, allowed research to develop toward synthetic materials, such as the rhBMP-2, which is the main morphogenetic protein inducer of bone tissue. (bvsalud.org)
  • Hence, it may be observed that these proteins play an important role in bone formation. (bvsalud.org)
  • Bone Morphogenetic Proteins. (bvsalud.org)
  • Recombinant SARS-CoV-2 antigens, such as the Spike (S) glycoprotein or Nucleocapsid (N), are used for the isolation of target-specific neutralizing antibodies. (invivogen.com)
  • abstract = "Fertilin α and β are members of the MDC (metalloproteinase-like, disintegrin-like, cysteine-rich) protein family and are expressed on the sperm surface where they have been proposed to play a role in mammalian fertilization. (edu.au)
  • Homeodomain-interacting protein kinases (HIPKs) belong to the CMGC kinase family and are closely related to dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs). (nature.com)
  • The ABA-bound receptor proteins interact with the members of the clade A subfamily of PP2Cs (e.g, ABI1, ABI2, and HAB1). (molcells.org)
  • CD137 is a type I membrane protein and a member of the TNF receptor superfamily. (adipogen.com)
  • Therefore, the risk of altering protein function, blocking other epitopes, or decreasing solubility is minimized. (sigmaaldrich.com)
  • Production of perdeuterated proteins for neutron-scattering experiments. (lu.se)
  • This methodology facilitates the isolation and recovery of the fusion peptide from the host cells by exploiting the physico-chemical properties of the fusion partner. (justia.com)
  • Each protein fusion tag was cloned separately on the C terminal of GST. (sigmaaldrich.com)
  • Recombinant Mpn HPRT was expressed as an N-terminal fusion protein with a 6 × histidine tag and a tobacco etch virus (TEV) cleavage site at the N-terminus, and was purified to >98% purity by metal affinity chromatography, see more as assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis (data not shown). (bcl-2protein.com)
  • For less potent cell lines, protein levels are typically ~0.1 mG/L. The presence of the SV40 replication origin will result in high levels of DNA replication in SV40 replication permissive COS cells. (sigmaaldrich.com)
  • Purified GST fusion proteins of the N and C termini of Na v 1.3 pull down contactin from lysates of transfected HEK 293 cells. (jneurosci.org)
  • Cells infected with this recombinant virus (gG-VAC) synthesized glycosylated proteins of 48K, 57K and 61K mol. (microbiologyresearch.org)
  • proteins in HSV-1-infected cells. (microbiologyresearch.org)