• Immunohistochemical analysis were performed using the monoclonal antibody CD34 to determine the angiogenic activity, and the monoclonal antibodies 3E6 and ER-PR8 to assess PSMA and PSA expression, respectively. (biomedcentral.com)
  • Cells were incubated with -SMA and TNFRSF1A Collagen I were visualized with an over night with specific fluorochrome main antibodies including -SMA (Abcam, Galactose 1-phosphate Potassium salt United States), Collagen I (Affinity, China) at a concentration of 1 1:100. (healthanddietblog.info)
  • Aberrant prostate growth arises as a consequence of changes in the balance between cell proliferation and cell death [ 3 ]. (biomedcentral.com)
  • Of interest, MM-derived MVs were enriched with the biologically active form of CD147, a transmembrane molecule previously shown by us to be crucial for MM cell proliferation. (oncotarget.com)
  • In summary, this study demonstrates the significance of MV shedding and MV-mediated intercellular communication on malignant plasma cell proliferation, and identifies the role of MV-enriched CD147 in this process. (oncotarget.com)
  • Cell proliferation was recognized by CCK8 (Dojindo, Japan) following a manufacturers protocol. (healthanddietblog.info)
  • There is a growing literature that tumor cells release biologically active microvesicles (MVs) that modify both local and distant microenvironments. (oncotarget.com)
  • We now report that MT-Exendin mice develop extensive tissue lymphocytic infiltration with increased numbers of CD4 + and CD8a + cells in the liver and/or kidney and increased numbers of B220 + cells present in the pancreas and liver. (diabetesjournals.org)
  • The GB hepatitis viruses (GBV-A, GBV-B and GBV-C). The GB hepatitis viruses were cloned recently and preliminary genomic characterization shows that they are related to other positive-stranded RNA viruses with local regions of sequence identity with various flaviviruses. (medmuv.com)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
  • Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. (lookformedical.com)
  • The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease. (lookformedical.com)
  • A group of heterogeneous lymphoid tumors representing malignant transformations of T-lymphocytes. (lookformedical.com)
  • B-cell lymphoid tumors that occur in association with AIDS. (lookformedical.com)
  • The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
  • If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. (lookformedical.com)
  • The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
  • The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1). (lookformedical.com)
  • Cloning Complementary DNA Avoids Introns Most eukaryotic genes have intervening sequences of non-coding DNA (introns) between the segments of coding sequence (exons). (binaryoptionsforex625.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. (lookformedical.com)
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • methods of heating a plasma by means of electromagnetic waves at various plasma resonance frequencies. (binaryoptionsforex625.com)
  • The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Cells grown in vitro from neoplastic tissue. (lookformedical.com)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
  • Malignant lymphoma composed of large B lymphoid cells whose nuclear size can exceed normal macrophage nuclei, or more than twice the size of a normal lymphocyte. (lookformedical.com)
  • Western Blot Analysis Lung cells and cultured cells were extracted with RIPA buffer and then centrifuged at 15,000?rpm, 4C for 15?min, the supernatant was collected. (healthanddietblog.info)
  • Herein we present clear evidence that not only do both patient MM cells and human MM cell lines (HMCLs) release MVs, but that these MVs stimulate MM cell growth. (oncotarget.com)
  • The observation of this intercellular communication and that MM cells modify the microenvironment is highly suggestive that one of the first biologically relevant changes necessary for progression of MGUS or SMM to MM is for a member(s) of the clone to evolve and acquire the molecular changes needed to reprogram its immediate microenvironment into a supportive, growth-promoting microenvironment. (oncotarget.com)
  • In this study, our goals were to determine if MM cells release MVs, and if so, begin to characterize their biologic activity. (oncotarget.com)
  • This deregulation may result in production of prostate specific markers such as the secreted protease prostate-specific antigen (PSA) and the cell surface prostate-specific membrane antigen (PSMA) [ 4 ]. (biomedcentral.com)
  • R.I. FRESHNEY: "Culture of Animal Cells: A Manual of Basic Technique", 1987, ALAN R. LISS, INC. (sumobrain.com)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • These data suggest that these markers are regulated differentially and the difference in their expression showed a correlation with malignant transformation. (biomedcentral.com)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
  • It is likely that polyclonal B-cell lymphoproliferation in AIDS is a complex result of EBV infection, HIV antigenic stimulation, and T-cell-dependent HIV activation. (lookformedical.com)
  • They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. (lookformedical.com)
  • One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). (lookformedical.com)
  • Cold Spring Harbor Laboratory Press (CSHLP) announced the release of T-Cell Memory, available on its website in hardcover and eBook formats. (cshlpress.com)
  • Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)
  • After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • A form of undifferentiated malignant LYMPHOMA usually found in central Africa, but also reported in other parts of the world. (lookformedical.com)