The rat G-protein-coupled receptor kinase 6 (GRK6) cDNA was cloned from rat brain tissue by a combination of reverse-transcription polymerase chain reactions (RT-PCR), based on homology to the cloned human GRK6, and rapid amplification of cDNA ends (RACE-PCR). (nih.gov)
Using a cDNA clone for glyoxysomal malate synthase from cucumber cotyledons as a probe, it was shown that malate synthase gene transcripts in cucumber cotyledons increased in amount to a peak on the 3rd or 4th day of germination and then declined. (eurekamag.com)
Hybridization of restriction fragments with acetate‐specific mRNA or cDNA has been used to delimit the transcribed region(s) of each clone. (psu.edu)
To identify the photoreceptive molecule that regulates photoperiodic responses, in the present study, we have cloned and characterized the cDNA encoding an opsin gene expressed in the ayu brain, a putative site of the photoreceptor for photoperiodism. (bioone.org)
In this investigation, we cloned a novel cDNA for mouse brain sialidase and expressed the cDNA in COS-7 cells. (elsevierpure.com)
We describe here the cloning of the cDNA encoding human alpha-endosulfine, a 13-kDa peptide that is a putative candidate for such a role. (ox.ac.uk)
Characterization2
We report the cloning and characterization of the gene ABNORMAL FLORAL ORGANS (AFO), which is required for normal flower development in Arabidopsis. (edu.sa)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)
You will also find learning and support resources for molecular biology techniques and methods, available to you at your fingertips. (thermofisher.com)
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Gene4
Chemically synthesised oligonucleotides can also be used if the target sequence size does not exceed the limit of chemical synthesis.Isolation of insert can be done by using shotgun cloning,c-DNA clones,gene machines(artificial chemical synthesis). (bionity.com)
A second gene-specific primer was designed and used in 5ʹ RACE PCR to clone the 5՛ region. (microbiologyresearch.org)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
The AFO gene was cloned and is predicted to encode a novel protein of 229 amino acids. (edu.sa)
MRNA2
We report the molecular cloning of DNA complementary to human Hp mRNA. (rti.org)
Four Neurospora crassa genomic clones have been selected as hybridizing much more strongly to labeled mRNA isolated from acetate‐grown mycelium than to mRNA from sucrose‐grown mycelium. (psu.edu)
Expression4
In practice, however, a number of other features are desired and a variety of specialised cloning vectors exist that allow protein expression, tagging, single stranded RNA and DNA production and a host of other manipulations that are useful in downstream applications. (bionity.com)
1994), Molecular cloning and expression of a human hea. (xenbase.org)
Molecular cloning and expression of a human heart inward rectifier potassium channel. (xenbase.org)
Human alpha-endosulfine, a possible regulator of sulfonylurea-sensitive KATP channel: molecular cloning, expression and biological properties. (ox.ac.uk)
Malate synthase1
Molecular transformation of a Neurosporaacu‐5 mutant and of an Aspergillus nidulans acuE mutant by DNA of clone 2 and clone 1, respectively, strongly suggests that clone 2 codes for acetyl‐coenzyme A synthetase and that clone 1 codes for malate synthase. (psu.edu)
Restriction3
In the classical restriction and ligation cloning protocols, cloning of any DNA fragment essentially involves four steps: fragmentation, ligation, transfection, and screening/selection. (bionity.com)
Viral DNA isolated from a single wart was partially methylated at only one out of the four HpaII sites, d(C-C-G-G). Recognition sites for Bg/I, Bg/II, PstI and PvuII restriction endonucleases were located on the cloned genome. (pasteur.fr)
GenBuilder™ seamless cloning technology offers high efficiency, quick and direct cloning, bypassing all the tedious procedures of traditional cloning that often involve restriction, ligation, and sometimes phosphorylation. (genscript.com)
Amplify1
Cloning is frequently employed to amplify DNA fragments containing genes, but it can be used to amplify any DNA sequence such as promoters , non-coding sequences, chemically synthesised oligonucleotides and randomly fragmented DNA. (bionity.com)
Homology1
As part of a directed effort to discover novel G protein-coupled receptors through homology searching of expressed sequence tag databases, we identified a partial clone (GPCR97) that had significant homology to biogenic amine receptors. (aspetjournals.org)
Genomic1
Subsequent cloning and sequencing of gcsap fragments obtained from PCR with genomic DNA revealed a 73 bp intron beginning at nt 728. (microbiologyresearch.org)
Fragments2
Preparation of DNA fragments for cloning can be accomplished in a number of alternative ways. (bionity.com)
GeneArt Gibson Assembly cloning is the optimal choice for building large and demanding constructs from multiple DNA fragments. (thermofisher.com)
Nuclear transfer2
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)
In the first part of How to Clone a Mammoth , Shapiro describes how the scientific community moved from the early (naïve) idea of finding an outstandingly well-preserved sample and proper cloning by somatic nuclear transfer to creating a mammoth-like elephant by genome editing. (molecularecologist.com)
Biological2
Cloning is utilized in a wide array of biological experiments and technological applications such as large scale protein production. (bionity.com)
1. Cloning is an umbrella term traditionally used to describe different processes for duplicating biological material. (who.int)
Plasmid1
The entire genome of human papilloma virus type 1a was cloned in Escherichia coli using the plasmid pBR322 as vector. (pasteur.fr)
19971
WHA50.37 of 1997 argues that human cloning is ethically unacceptable and contrary to human integrity and morality. (who.int)
Antibiotic resistance1
Modern cloning vectors include selectable markers (most frequently antibiotic resistance markers) that allow only cells in which the vector, but not necessarily the insert, has been transfected to grow.Additionally, the cloning vectors may contain colour selection markers which provide blue/white screening (via α-factor complementation) on X-gal medium. (bionity.com)
Human8
We obtained a clone of 2817 bp with an open reading frame of 1731 bp encoding for a protein of 576 amino acids that is 96.7% identical and 97.9% similar to its human counterpart. (nih.gov)
The GPCR97 clone was used to probe a human thalamus library, which resulted in the isolation of a full-length clone encoding a putative G protein-coupled receptor. (aspetjournals.org)
2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
General Assembly the adoption of a declaration on human cloning by which Member States were called upon to prohibit all forms of human cloning inasmuch as they are incompatible with human dignity and the protection of human life. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
WHA50.37, which states "the use of cloning for the replication of human individuals is ethically unacceptable and contrary to human integrity and morality. (who.int)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
Differences2
What are the differences between the GenBuilder™ kit and the PCR Cloning Kit? (genscript.com)
The data collected from the resultant spectra were used to assess differences among clones and among ramets of a single clone and to determine the mechanism governing the observed differences. (who.int)
Optimal1
The recombinant protein named FoxTPI was purified (97% purity) showing a molecular mass of 27 kDa, with optimal activity at pH 8.0 and and temperature of 37 °C. The values obtained for Km and Vmax using the substrate GAP were 0.47 ± 0.1 mM, and 5331 µmol min-1 mg-1, respectively. (bvsalud.org)
Transformation1
Electroporation is used when extremely high transformation efficiencies are required, as in very inefficient cloning strategies. (bionity.com)
Receptor1
The existence of the histamine H 3 receptor was demonstrated pharmacologically 15 years ago, yet despite intensive efforts, its molecular identity has remained elusive. (aspetjournals.org)
Sequence2
[Molecular cloning] refers to the procedure of isolating a defined DNA sequence and obtaining multiple copies of it in vivo . (bionity.com)
The identity of the clone and the accuracy of the sequence was verified by direct aa sequencing of the tryptic peptides derived from the purified rat liver enzyme. (lu.se)
Research2
With Applied Biosystems and Invitrogen products for PCR and cloning, there is no wondering whether they will propel your research forward. (thermofisher.com)
Despite the fact that my research is not related to cloning a mammoth, I had to learn about the cloning efforts, so that I can answer the numerous questions. (molecularecologist.com)
Cells1
That means that, while these mummies might appear - superficially - to be the most likely source of an intact cell suitable for cloning, their remains may actually be the worst place to look for such cells. (molecularecologist.com)
Cell3
The primary structure of HH- IRK1 is homologous to that of the IRK1 channel cloned from a mouse macrophage -like cell line. (xenbase.org)
The recombinant protein displaces binding of the sulfonylurea [3H]glibenclamide to beta cell membranes, inhibits cloned KATP channel currents, and stimulates insulin secretion. (ox.ac.uk)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)
State2
Intestinal carriage of prolonged spectrum β-lactamase (ESBL)-producing Escherichia coli is a frequent, rising and worrying phenomenon, however little is understood in regards to the molecular state of affairs and the evolutionary forces at play. (bioconstructor.com)
It's essential reading for everybody interested in understanding the current state, future prospects, and all the challenges of cloning a mammoth. (molecularecologist.com)
High2
Whatever your workflow needs, our comprehensive and diverse molecular solutions are designed for high performance, time savings, and reliability for your PCR and cloning success. (thermofisher.com)
The multivariate analyses conducted on the data further emphasized the results obtained from ANOVA and a high degree of success (90%) was achieved in classifying seed samples to their source clone. (who.int)
Full length1
One of the clones, pULB1148, carries a full length copy coding for both alpha 2 and beta polypeptides. (rti.org)
Common1
Three embryonic class II myosin heavy chains (MYHs) were cloned from the common carp ( Cyprinus carpio L.), MYH emb1 ,MYH emb2 and MYH emb3 . (biologists.com)
Learn1
Learn PCR and cloning at your own pace and on your own time. (thermofisher.com)
Question1
When I explain that I study the woolly mammoth, sooner or later (and usually right away) comes the question, "Are you going to clone a mammoth? (molecularecologist.com)
Copy1
A clone is an organism that is a genetic copy of an existing one. (who.int)
General2
From childish excitement to real scientific interest, the idea of cloning a mammoth raises general enthusiasm. (molecularecologist.com)
In How to Clone a Mammoth , Shapiro tries to merge the general (and personal) enthusiasm for de-extinction with all the challenges that science has to overcome to move de-extinction from science fiction to reality. (molecularecologist.com)