Clinical specimens gram positive cocci. Medical search. Frequent questions

Inadequate therapy against these anaerobic bacteria may lead to clinical failures. (medscape.com)
Anaerobic gram-positive cocci are usually recovered mixed with other anaerobic or aerobic bacteria from infections at different sites of the body. (medscape.com)
Martin reported that anaerobic cocci were isolated in 8.5-31% of clinical specimens that yielded any anaerobic bacteria at the Mayo Clinic. (medscape.com)
Anaerobic and facultative gram-positive cocci are often isolated from clinical specimens mixed with other anaerobic or aerobic bacteria and, on rare occasions, are isolated as the sole pathogen. (medscape.com)
Collecting anaerobic bacteria specimens is important because documentation of an anaerobic infection is through culture of organisms from the infected site. (medscape.com)
Samples from the salad showed the presence of gram-positive cocci bacteria in clusters. (chezbebeny.com)
Staphylococcus aureus is a type of bacteria that belongs to the genus Staphylococcus, which consists of spherical-shaped, Gram-positive organisms that usually form clusters resembling grapes. (stemcelldaily.com)
Gatifloxacin is an 8-methoxy fluoroquinolone that shows a broad spectrum and increased antibacterial activities against Gram-positive cocci bacteria, anaerobes, chlamydias and mycoplasmas. (bmj.com)
While gram-positive and gram-negative bacteria showed low susceptibility to Ampicillin, Tetracycline and Cotrimoxazole, the GNR were susceptible to Gentamicin and third-generation cephalosporins. (biomedcentral.com)
Sepsis is a blood stream infection usually caused by pathogenic bacteria with the diagnosis often beginning with clinical suspicion [ 3 ]. (biomedcentral.com)
Gram stain is the most common stain used in microbiology to identify bacteria. (medscape.com)
Approximately 40%-80% of the dry weight of gram-positive bacteria consists of peptidoglycan, while gram-negative bacteria have only a single layer of peptidoglycan. (medscape.com)
When ethanol is used as decolorizing agent, gram-negative bacteria lose their outer membrane because of the higher content of lipid, allowing crystal violet complex to leak through the cell wall. (medscape.com)
Organisms without a cell wall (eg, Mycoplasma species) and small bacteria (eg, Chlamydia and Rickettsia species) do not stain with Gram stain. (medscape.com)
Other important enteric gram-negative bacteria include Enterobacter species, Klebsiella pneumoniae , Escherichia coli , Serratia marcescens , Proteus species, and Acinetobacter species. (merckmanuals.com)
The prevalence of antimicrobial resistance in some Egyptian hospitals was high among Gram-negative, Gram-positive bacteria, and candida spp. (biomedcentral.com)
Regarding to postoperative pain, the most prevalent bacteria were Gram-positive cocci. (bvsalud.org)
Flowchart that can be used to identify bacteria by using Gram-positive stain. (cdc.gov)
Many infections caused by aerobic and anaerobic gram-negative bacteria resistant to some cephalosporins respond to cefoxitin for injection, USP. (wikidoc.org)
Colistin is an old drug, and its use has recently resurged because of increasing antibiotic resistance in gram-negative bacteria such as Acinetobacter baumannii . (pfmjournal.org)
Although the colistin resistance rates in gram-negative bacteria are currently not high, many colistin-resistant isolates are being identified and the possibility of horizontal transmission of colistin resistance has increased because of the plasmid-borne colistin resistance gene mcr-1 (mobilized colistin resistance). (pfmjournal.org)
Staphylococcus is a genus of Gram-positive bacteria known for their spherical or grape-like clustered shape under a microscope. (medicallabscientist.org)

Therefore, it is essential to understand the bacteriology, clinical diseases and diagnosis of Staphylococcus aureus in order to develop effective strategies to combat this formidable pathogen. (stemcelldaily.com)
Gram-positive cocci, including coagulase-negative staphylococci, Staphylococcus aureus , Streptococcus pneumoniae and other Gram-positive cocci, accounted for 42.3% of isolates. (who.int)
ABSTRACT We evaluated the utility of 2 methods for detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from signal-positive blood culture bottles: loop-mediated isothermal amplification (LAMP) assay, and direct cefoxitin disk diffusion (DCDD) test using a 30 g cefoxitin disk. (who.int)
Sur 60 hémocultures positives pour les cocci à Gram positif en grappes, l'amplification isotherme induite par boucle (au moyen du dépistage des gènes Fem A et Mec A) a montré une sensibilité et une spécificité de 100 % pour l'identification de Staphylococcus aureus résistant et sensible à la méthicilline. (who.int)
Gram-positive organisms such as Staphylococcus aureus and Streptococcus species are the most commonly isolated organisms in patients with pyogenic vertebral infections. (wheelessonline.com)
Examples of gram-positive cocci include Staphylococcus and Streptococcus species. (medscape.com)
Staphylococcus aureus is a Gram-positive spherically shaped bacterium , a member of the Bacillota , and is a usual member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin . (mdwiki.org)
Staphylococcus Staphylococcus Staphylococcus is a medically important genera of Gram-positive, aerobic cocci. (lecturio.com)
S. epidermidis S. epidermidis A species of staphylococcus that is a spherical, non-motile, gram-positive, chemoorganotrophic, facultative anaerobe. (lecturio.com)
Staphylococcus aureus is a spherical bacterium, commonly referred to as a coccus. (medicallabscientist.org)
Staphylococcus aureus is classified as a Gram-positive bacterium. (medicallabscientist.org)
Staphylococcus aureus has a thick, peptidoglycan-rich cell wall, which contributes to its Gram-positive staining. (medicallabscientist.org)
Staphylococcus aureus is positive for coagulase production. (medicallabscientist.org)
Staphylococcus aureus is positive for catalase production. (medicallabscientist.org)

Anaerobic gram-positive cocci are the second most frequently recovered anaerobes and account for approximately one quarter of anaerobic isolates. (medscape.com)
Incidence of clinical isolates from the pus, bile and catheter tip during about latest twenty years was investigated retrospectively in order to know the actual condition of hospital infection in our ward. (nii.ac.jp)
Gram positive cocci (GPC) were the predominant isolates with Coagulase positive (32.2%) and Coagulase-negative (28.7%) Staphylococci accounting for 60.9% of the total isolates. (biomedcentral.com)
Gram negative rods (GNR) comprised 39.1% of all isolates with Klebsiella, E . coli and Salmonella being the most common organisms isolated. (biomedcentral.com)
We describe the clinical presentation of three cases and compared the S. zooepidemicus isolates from the human cases with S. zooepidemicus isolates from horses to obtain insight into the epidemiology of S. zooepidemicus . (cdc.gov)
The purpose of this study was to characterize the clinical presentation of the disease caused by S. zooepidemicus , microbiologically characterize the isolated strains, and identify clonality of human isolates for comparison to equine isolates from contact horse stables or other horse farms of the surrounding area. (cdc.gov)
During the period from November 2020 to January 2021, one hundred microbial isolates were collected randomly from different specimens from some hospitals in Cairo, Egypt. (biomedcentral.com)
R. insidiosa in clinical isolates. (who.int)

Anaerobic gram-positive cocci that produce large amounts of lactic acid during the process of carbohydrate fermentation were reclassified as Streptococcus parvulus and Streptococcus morbillorum from Peptococcus or Peptostreptococcus . (medscape.com)
Les cocci à Gram positif, y compris les staphylocoques à coagulase négative, Staphylo- coccus aureus , Streptococcus pneumoniae et autres cocci à Gram positif représentaient 42,3 % des isolats. (who.int)
Methicillin-sensitive S. aureus , Streptococcus pneumoniae , and Haemophilus influenzae are most commonly implicated when pneumonia develops within 4 to 7 days of hospitalization, whereas P. aeruginosa , MRSA, and enteric gram-negative organisms become more common with increasing duration of hospitalization. (merckmanuals.com)
This activity describes the bacterium Streptococcus pyogenes (S. pyogenes) and reviewed the epidemiology, clinical manifestations of S. pyogenes infections, evaluation, and management of clinical infections caused by S. pyogenes. (nih.gov)

Coagulase-positive S. aureus is among the most ubiquitous and dangerous human pathogens, for both its virulence and its ability to develop antibiotic resistance. (merckmanuals.com)
S. aureus is one of the leading pathogens for deaths associated with antimicrobial resistance and the emergence of antibiotic-resistant strains, such as methicillin-resistant S. aureus (MRSA), is a worldwide problem in clinical medicine . (mdwiki.org)
A positive result indicates the presence of coagulase, a key characteristic of S. aureus. (medicallabscientist.org)
S. aureus is Gram-positive, and this characteristic is determined through a simple Gram stain procedure. (medicallabscientist.org)

[ 11 ] The ability of anaerobic gram-positive cocci and microaerophilic streptococci to produce capsular material is an important virulence mechanism, but other factors may also influence the interaction of these organisms in mixed infections. (medscape.com)
Staphylococci are gram-positive aerobic organisms. (merckmanuals.com)
Gram-positive organisms are seen as blue/purple (see image below). (medscape.com)
Gram-negative organisms are seen as pink/red (see image below). (medscape.com)
Gram-variable organisms are those that cannot be grouped as either negative or positive. (medscape.com)
The appearance of organisms that stain gram-positive or -negative means that the smear contains organisms. (medscape.com)
Bacilli are rod-shaped organisms, while cocci are spherical in shape. (medscape.com)
Gram-positive organisms have a higher content of peptidoglycan in their cell wall than gram-negative organisms. (medscape.com)
In contrast, in gram-positive organisms, because of the higher content of peptidoglycan in the cell wall, crystal violet is trapped inside the cell. (medscape.com)
Gram-negative organisms take the counterstain and stain pink/red. (medscape.com)
Gram-variable organisms include Actinomyces species. (medscape.com)

The rationale for choosing these 3 strains is to represent gram-positive cocci, gram-negative bacilli, and anaerobes with different oxygen requirements, covering the typical application range of the medium comprehensively as appropriate quality control strains. (mantacc.com)
Gram-negative bacilli. (medscape.com)
The organism is identified as gram-positive or -negative based on the color and as bacilli or cocci based on the shape. (medscape.com)
Gram-positive bacilli include Corynebacterium , Clostridium , and Listeria species. (medscape.com)
Gram-negative bacilli include Escherichia coli, Pseudomonas species, Proteus species, and Klebsiella species. (medscape.com)
Other possible microbiologic etiologies include Neisseria meningitidis, Listeria monocytogenes, and enteric gram-negative bacilli. (brainkart.com)
According to the results, it can be concluded that Fusobacterium nucleatum and Gram-negative bacilli were more related to pre-operative pain. (bvsalud.org)

It is especially suitable for respiratory, urogenital and intestinal specimens where survival of anaerobes needs to be ensured. (mantacc.com)

Clinically significant anaerobic cocci include Peptostreptococcus species, Veillonella species (gram-negative cocci), and microaerophilic streptococci (aerotolerant). (medscape.com)
Anaerobic gram-positive cocci include various clinically significant species of the genus Peptostreptococcus . (medscape.com)
Dysbiotic subgingival communities are characterized by a relative increase in abundance of anaerobic, Gram-negative bacterial species that can exploit protein-rich GCF as a nutrient source, promoting development of a proteolytic community phenotype. (biomedcentral.com)
Gram-negative cocci include Neisseria gonorrhoeae, Neisseria meningitides, and Moraxella species. (medscape.com)
40 observed that the number of bacterial species were greater in cases in which there were clinical manifestations as pain, edema, abscess, or fistula. (bvsalud.org)

Diagnosis is by Gram stain and culture. (merckmanuals.com)
A variation of Gram staining is called Atkin Gram stain, in which the primary stain is gentian violet and mordant is Atkin iodine. (medscape.com)
Gram stain is used to identify the organism causing a disease or pathological condition. (medscape.com)
CSF Gram stain reveals gram-positive cocci in pairs. (brainkart.com)
Flowchart of Gram stain morphology that can be used to identify aerobic Gram-positive cocci. (cdc.gov)
This means that it retains the crystal violet stain during the Gram staining process, indicating that it has a thick layer of peptidoglycan in its cell wall. (medicallabscientist.org)

Coagulase Negative, Coagulase Positive Staphylococci, Salmonella and Klebsiella were the aetiological agents of bloodstream infection among children at TTH. (biomedcentral.com)
Streptococci are gram-positive, catalase-negative, coagulase-negative cocci that occur in pairs or chains. (nih.gov)

Of 60 blood cultures positive for Gram-positive cocci in clusters, LAMP (via detection of the Fem A and Mec A genes) showed 100% sensitivity and specificity for identification of MRSA/MSSA. (who.int)
The cocci can appear as individual cells or in clusters. (medicallabscientist.org)

Coprococcus' - faecal coccus 'eutaktos' - orderly, well-disciplined (referring to the uniform reactions of the different strains) Holdeman, L. V. (wikipedia.org)
it contains the indole-positive, saccharolytic strains of the genus. (medscape.com)
There were 118 anaerobic bacterial strains detected in 66 (27.9%) of the 236 specimens. (clin-lab-publications.com)

Bacterial synergy, the presence of which is determined by mutual induction of sepsis enhancement, increased mortality, increased ability to induce abscesses, and enhancement of the growth of the bacterial components in mixed infections, is found between anaerobic gram-positive cocci and their aerobic and anaerobic counterparts. (medscape.com)
Out of 331 blood specimens cultured, the prevalence of confirmed bacterial sepsis was 25.9% (86/331). (biomedcentral.com)
Bacterial infections are often diagnosed on clinical grounds, supported by smears, culture, testing for immune responses (serology) and, increasingly, examining for nucleic acids. (pocketdentistry.com)

Des bacilles à Gram négatif étaient impliqués dans 42,3 autres pour cent des isolats, Pseudomonas aerug- inos étant l'isolat prédominant. (who.int)

If the organism culture is old, it may lose the peptidoglycan cell wall, causing a gram-positive organism to appear as gram-negative or gram-variable. (medscape.com)

Inoculate liquid specimens into an anaerobic transport vial or a syringe. (medscape.com)
RECARBRIO 1.25 grams for injection is supplied as sterile powder for constitution in a single-dose vial containing imipenem 500 mg (anhydrate equivalent), cilastatin 500 mg (free acid equivalent), and relebactam 250 mg (anhydrate equivalent). (nih.gov)
Each 1 gram vial contains cefotetan disodium equivalent to 1 gram cefotetan activity. (globalrph.com)

Diagnosis is suspected on the basis of clinical presentation and chest imaging and is confirmed by blood culture or bronchoscopic sampling of the lower respiratory tract. (merckmanuals.com)
Initial diagnosis is usually based on chest x-ray and clinical findings. (merckmanuals.com)
2. Have appropriate clinical specimens been obtained to establish a microbiologic diagnosis? (brainkart.com)
It is necessary to focus your microscope when viewing specimens to perform a proper analysis and give an accurate diagnosis. (cdc.gov)
Acute sinusitis is a clinical diagnosis. (medscape.com)
The diagnosis is based on clinical findings, blood cultures, and echocardiography Echocardiography Ultrasonic recording of the size, motion, and composition of the heart and surrounding tissues. (lecturio.com)

Blood culture and antimicrobial susceptibility testing results are usually available between 48-72 hours after the specimen is obtained and therefore initial antimicrobial treatment has been usually empirical with the aim that the most likely pathogens would be susceptible to the chosen drugs [ 5 ]. (biomedcentral.com)
VAP ⫽ ventilator-associated pneumonia P neumonia was the most common nosocomial frequently reported pathogens, but Gram-positive infection among patients in combined medical- pathogens are being reported with increasing fre- surgical ICUs in the National Nosocomial Infections quency. (marysfamilymedicine.org)
lance1 and for 30% of pathogens in patients in the In the past, Gram-negative aerobes were the most EPIC Study,2 which included pneumonia and other CHEST / 124 / 5 / NOVEMBER, 2003 Downloaded From: http://publications.chestnet.org/ on 10/07/2016 types of ICU-acquired infections. (marysfamilymedicine.org)

Documentation requires proper collection of appropriate specimens, expeditious transportation, and careful laboratory processing. (medscape.com)
The or- ferred to blood culture media and immedi- ganisms most commonly isolated from ately transported to the hospital's blood cultures are Gram-positive cocci in- microbiology laboratory. (who.int)
Laboratory values must all the time be interpreted within the context of clinical information derived from different sources, including the medical historical past and bodily examination. (atpobtvs.com)
Clinical histories and laboratory culture. (who.int)

Anaerobic, microaerophilic, and facultative gram-positive cocci have minor morphological differences. (medscape.com)

In the earlier period, gram negative rods were dominant in clinical specimens but recently gram positive cocci, especially MRSA, has become more important. (nii.ac.jp)
Other baseline variables associated with significantly higher clinical cure rates in MRSA pneumonia were single-lobe pneumonia, absence of ventila- tor-associated pneumonia, and absence of oncologic and renal comorbidities. (marysfamilymedicine.org)
In this retrospective analysis, initial therapy with linezolid was associated with significantly better survival and clinical cure rates than was vancomycin in patients with nosocomial pneumonia due to MRSA. (marysfamilymedicine.org)

In randomized comparative studies, Cefoxitin for injection, USP and cephalothin were comparably safe and effective in the management of infections caused by gram-positive cocci and gram-negative rods susceptible to the cephalosporins. (wikidoc.org)

Specimens must be obtained free of contamination. (medscape.com)
Specimens of the lower respiratory tract are difficult to obtain without contamination with indigenous florae. (medscape.com)
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by vancomycin-resistant Enterococcus DNA is unlikely. (mayocliniclabs.com)

Coprococcus (ATCC 27759) is a genus of anaerobic cocci which are all part of the human faecal flora, but rarely seen in human clinical specimens. (wikipedia.org)
Peptostreptococcus is the only genus among anaerobic gram-positive cocci encountered in clinical infections. (medscape.com)
Gas-liquid chromatography and biochemical tests are required for genus-level identification and separation of most anaerobic gram-positive cocci. (medscape.com)

CEFOTAN™ (cefotetan for Injection, USP) is supplied in vials containing 80 mg (3.5 mEq) of sodium per gram of cefotetan activity. (globalrph.com)
MEFOXIN contains approximately 53.8 mg (2.3 milliequivalents) of sodium per gram of cefoxitin activity. (rxlist.com)

The exact frequency of Peptostreptococcus infections is difficult to calculate because of inappropriate methods of collection, transportation, and cultivation of specimens. (medscape.com)

However, no gram negative rods was isolated in the last year (1987). (nii.ac.jp)

P micros has a smaller diameter than other anaerobic gram-positive cocci and usually forms short chains. (medscape.com)

Liquid or tissue specimens are always preferred to swabs. (medscape.com)
Simplify the transportation of clinical samples with Mantacc Stuart Transport Swabs. (mantacc.com)
The soft rayon tips of Mantacc swabs allow easy specimen collection while keeping microorganisms alive. (mantacc.com)
Tissue specimens obtained by scraping the base of the ulcer with a scalpel or by wound or bone biopsy are strongly preferred to wound swabs. (pdftreatment.com)

1. Is an antimicrobial agent indicated on the basis of clinical findings? (brainkart.com)
5. Is there clinical evidence (eg, from well-executed clinical trials) that antimicrobial therapy will confer clinical benefit for the patient? (brainkart.com)

It is often positive for catalase and nitrate reduction and is a facultative anaerobe that can grow without the need for oxygen. (mdwiki.org)

Clinical symptoms and signs in patients are onto chocolate agar, blood agar and eosin not sufficiently reliable to predict bacter- methylene blue agar plates. (who.int)
His condition was septic with clinical symptoms of meningismus and pulmonary congestion. (cdc.gov)
The diag- nosis of diabetic foot infection is based on the clinical signs and symptoms of local inﬂammation. (pdftreatment.com)

Staining of the CSF revealed gram-positive cocci in chains with a considerable number of polymorphonuclear cells on the microscopic examination. (cdc.gov)
All benign lesions showed positive immunoreaction, with the staining index varying from 6-9, except lactating adenoma. (ijpmonline.org)
Among the IDC (NOS) lesions, six cases of grade III breast carcinoma exhibited a positive immunohistochemical reaction, the staining index of which varied from 2-6. (ijpmonline.org)

Coprococcus includes those gram-positive, anaerobic cocci that actively ferment carbohydrates, producing butyric and acetic acids with formic or propionic and/or lactic acids. (wikipedia.org)

This video gives a brief overview of how to focus on a specimen using a compound microscope. (cdc.gov)

The colonies were positive for catalase. (chezbebeny.com)
S. pyogenes is a gram-positive, catalase-negative, oxidase negative, β-hemolytic streptococci. (nih.gov)

In the present case, x-ray findings indicated gas formation and additionally, Klebsiella ornithinolytica was recovered from surgical specimens. (faoj.org)

The main clinical signs were hyperthermia or hypothermia and respiratory distress. (bvsalud.org)

Sputum and chest specimens were from COVID-19 patients. (biomedcentral.com)

Pleural fluid specimens from 236 patients were inoculated on Schaedler agar. (clin-lab-publications.com)

Isolating them requires appropriate methods of specimen collection, transportation, and cultivation. (medscape.com)
Methods The study was an open clinical trial evaluating the effectiveness of gatifloxacin with 200 mg doses twice a day for 7 days against male NGU. (bmj.com)
Core science job aids are intended to improve skills, methods, and procedures in clinical and public health laboratories. (cdc.gov)

Active surveil ance, whereby symptomatic patients from tip culture was positive for Enterococcus faecalis and the the haemodialysis unit had specimens col ected for blood culture was positive for bacteraemia with R. insidi- blood culture and sensitivity testing, was initiated, and osa . (who.int)

Mannitol Salt Agar Intended Use Mannitol Salt Agar is used for the selective isolation and enu- meration of staphylococci from clinical and nonclinical materials. (chezbebeny.com)

Dr. Nagel added that, with this product, there is also "diminished sensitivity and specificity directly from specimens. (contagionlive.com)

Transport tissue specimens in an anaerobic jar or a sealed plastic bag rendered anaerobic. (medscape.com)

The usual adult dosage range of cefoxitin is 1 gram to 2 grams every six to eight hours. (rxlist.com)

Other specimens can be collected from abscess contents, from deep aspirates of wounds, and by special techniques, such as transtracheal aspirates or direct lung puncture. (medscape.com)

Coprococcus eutactus is an obligately anaerobic, nonmotile, gram-positive coccus occurring in pairs or chains of pairs. (wikipedia.org)

En parallèle, une identification microbiologique normalisée et un test de sensibilité à l'oxacilline par PCR visant l'amplification du gène Mec A ont été réalisés. (who.int)

P magnus has a larger diameter than other anaerobic gram-positive cocci. (medscape.com)

It is named after Christian Gram, who first developed the technique to identify the organism responsible for pneumonia. (medscape.com)

Or is it prudent to wait until such clinical findings become apparent? (brainkart.com)
Important clinical exam findings include a new or changed heart murmur and common extra-cardiac signs, such as Osler nodes, Janeway lesions, splinter hemorrhages, and Roth spots. (lecturio.com)

Anatomy16

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