Cleaves both strands of dna single strand specific dna and rna endonucleases. Medical search. Frequent questions

Protean offers Non-specific Endonuclease isolated from Serratia marcescens, which is a highly processive enzyme active under a variety of conditions and temperatures - Endonuclease S. marcescens. (pharma-industry-review.com)
The Endonuclease S. marcescens enzyme supplied by Protean, as well as all other enzymes manufactured by the company, is produced in a certified environment under ISO 9001 and ISO 13485 standards and fully approved for use in downstream GMP certified processes. (pharma-industry-review.com)
The Protean Non-specific Endonuclease S. marcescens enzyme is ideally suitable for pharmaceutical and biopharmaceutical manufacturing cGMP applications. (pharma-industry-review.com)
Researchers supply cells with a template containing the desired sequence, make a targeted double-strand break with the Cas9 enzyme, and then wait to see whether homology-directed repair incorporates the template to reconnect the strands. (pharmaceuticalintelligence.com)
They then tethered Cas9 to an enzyme called cytidine deaminase that directly catalyzes conversion of C to U (essentially an equivalent of thymine, T), without DNA cleavage. (pharmaceuticalintelligence.com)
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated enzyme Cas9 is an RNA-guided endonuclease that uses RNA-DNA base-pairing to target foreign DNA in bacteria. (nature.com)
One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer. (neb.com)
EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation (3). (neb.com)
Monarch DNase I is optimized for use in this workflow, and is available for purchase as a component of the Monarch Total RNA Enzyme Pack, ( NEB #T2019 ). (neb.com)
DNA is a nucleic acid which is capable of duplicating itself via the enzyme known as DNA polymerase. (wikibooks.org)
EcoRI (which is pronounced as "eco R one") is a restriction endonuclease enzyme that is found in E. coli bacteria. (vedantu.com)
It's a restriction enzyme that cuts/cleaves DNA double helices into pieces at particular sites as a result of the restriction-modification mechanism. (vedantu.com)
The Eco portion of the enzyme's name comes from the species where it was isolated - "E" stands for "Escherichia" and "co" stands for "coli" - while the R stands for the specific strain, in this case, RY13, and the I stands for "first-ever enzyme extracted from this strain. (vedantu.com)
Eco-RI is a type II restriction enzyme that binds to double-stranded DNA and cleaves it at particular base pairs. (vedantu.com)
Natural restriction endonucleases are classified into four classes based on their composition and enzyme cofactor needs, as well as the nature of their target sequence and the position of their DNA cleavage site relative to the target sequence (Types I, II, III, and IV). (vedantu.com)
Restriction enzyme DNA sequence analyses, on the other hand, reveal significant variation, implying that more than four types exist. (vedantu.com)
Thermo Scientific restriction enzyme reaction buffers produce mixtures of BSA, which improves the stabilization of several enzymes and links contaminants in DNA preparations, ensuring consistent results. (vedantu.com)
Each enzyme cleaves at a specific four to eight-base pair sequence (a restriction site), producing DNA segments called restriction fragments. (pharmacy180.com)
A restriction fragment length polymorphism (RFLP) is a genetic variant that can be observed by cleaving the DNA into restriction fragments using a restriction enzyme. (pharmacy180.com)

The approach utilises the CRISPR-Cas9 gene editing system, coupled with libraries of single guide RNAs (sgRNAs), which are designed to target every gene in the genome. (wikipedia.org)
The clustered regularly interspaced short palindrome repeats (CRISPR)/Cas9 system is a gene-editing technology that can introduce double-strand breaks (DSBs) at a target genomic locus. (wikipedia.org)
By using a single guide RNA (sgRNA), the endonuclease Cas9 can be delivered to a specific DNA sequence where it cleaves the nucleotide chain. (wikipedia.org)
To date, CRISPR/Cas9 genome-editing approaches have relied on a cellular mechanism called homology-directed repair, which is triggered by double-strand breaks in DNA. (pharmaceuticalintelligence.com)
First, they inactivated part of Cas9 so that it couldn't make the double-strand break. (pharmaceuticalintelligence.com)
The team again modified Cas9, this time so that it would create a nick in the nonedited, G-containing strand, while leaving the edited, U-containing strand intact. (pharmaceuticalintelligence.com)
Cas9-guide RNA complexes are also effective genome engineering agents in animals and plants. (nature.com)
Here we use single-molecule and bulk biochemical experiments to determine how Cas9-RNA interrogates DNA to find specific cleavage sites. (nature.com)
We show that both binding and cleavage of DNA by Cas9-RNA require recognition of a short trinucleotide protospacer adjacent motif (PAM). (nature.com)
Non-target DNA binding affinity scales with PAM density, and sequences fully complementary to the guide RNA but lacking a nearby PAM are ignored by Cas9-RNA. (nature.com)
These results reveal how Cas9 uses PAM recognition to quickly identify potential target sites while scanning large DNA molecules, and to regulate scission of double-stranded DNA. (nature.com)
Figure 1: DNA curtains assay for target binding by Cas9-RNA. (nature.com)
Figure 2: Cas9-RNA remains bound to cleaved products and localizes to PAM-rich regions during the target search. (nature.com)
Figure 3: Cas9-RNA searches for PAMs and unwinds dsDNA in a directional manner. (nature.com)
RNA-guided human genome engineering via Cas9. (nature.com)
Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. (nature.com)
Research studies have reported that an extended secondary DNA on the guide crRNA for Cas12a or a hairpin RNA structure added to the sgRNA for Cas9 increases the efficiency and specificity of gene editing. (biorxiv.org)
These companies are using a variety of approaches, including the use of RNA interference (RNAi), antisense oligonucleotides (ASOs), and gene editing technologies such as CRISPR/Cas9. (inventiv.org)
In this report, we described the application of CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (endonuclease) system for efficient genetic editing in E. tenella , and showed that the CRISPR/Cas9 system mediates site-specific double-strand DNA breaks with a single guide RNA. (biomedcentral.com)
The CRISPR/Cas9 system introduces site-specific double-strand DNA breaks (DSBs) with endonuclease Cas9 in a target sequence that is homologous to the single guide RNA (sgRNA). (biomedcentral.com)

The deoxyribose sugars form the structural backbone for DNA via a phosphodiester bond between the 3' carbon of one nucleotide and the 5' carbon of the next. (wikibooks.org)
Vectors must be capable of autonomous replication within the host cell, must contain at least one specific nucleotide sequence recognized by a restriction endonuclease, and must carry at least one gene that confers the ability to select for the vector such as an antibiotic resistance gene. (pharmacy180.com)
It ideally contains a copy of every DNA nucleotide sequence in the genome. (pharmacy180.com)
A probe is a small piece of RNA or single-stranded DNA (usually labeled with a radioisotope, such as 32P, or another recognizable compound, such as biotin or a fluorescent dye) that has a nucleotide sequence complementary to the DNA molecule of interest (target DNA). (pharmacy180.com)
PCR permits the synthesis of millions of copies of a specific nucleotide sequence in a few hours. (pharmacy180.com)
We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
A region of a dsRNA sequence with a length of 19 nucleotides or more is complementary enough to a target RNA nucleotide to trigger destruction by the RNAi mechanism. (inventiv.org)
Dicer cleaves long dsRNA molecules into short, 21-22 nucleotide duplexes which are subsequently unwound by the RISC to yield mature siRNAs. (biomedcentral.com)

Furthermore, double-strand DNA has relatively small grooves where damaging enzymes can't attach, making it more difficult for them to 'attack' the DNA. (wikibooks.org)
Double-stranded RNA , on the other hand, has much larger grooves, and therefore, it is more subject to being broken down by enzymes. (wikibooks.org)
All enzymes recognize certain short DNA sequences and cleave DNA into specific pieces with 5'-phosphates at the ends. (vedantu.com)
single function (restriction digestion) enzymes do not require methylase. (vedantu.com)
Conventional restriction endonucleases from Ecori Thermo Scientific are a wide set of high-quality restriction enzymes that have been designed to function in one of the Five Buffer System's buffers. (vedantu.com)
Restriction endonucleases are bacterial enzymes that cleave double-stranded DNA (dsDNA) into smaller fragments. (pharmacy180.com)
Restriction enzymes form either staggered cuts (sticky ends) or blunt-end cuts on the DNA. (pharmacy180.com)

However, several problems remain and hinder efficient and specific editing of target genomic loci. (molcells.org)
A genomic library is a collection of fragments of dsDNA obtained by digestion of the total DNA of the organism with a restriction endonuclease and subsequent ligation to an appropriate vector. (pharmacy180.com)
Comparative-genomic analysis of CRISPR and cas genes leads to the hypothesis that the CRISPR-Cas system (CASS) is a mechanism of defense against invading phages and plasmids that functions analogously to the eukaryotic RNA interference (RNAi) systems. (biomedcentral.com)

That is to say why has evolution favored a DNA world over an RNA world given that the two molecules are so similar structurally? (wikibooks.org)
Prokaryotic organisms normally contain small, circular, extrachromosomal DNA molecules called plasmids that can serve as vectors. (pharmacy180.com)
In contrast, complementary DNA (cDNA) libraries contain only those DNA sequences that are complementary to messenger RNA (mRNA) molecules present in a cell and differ from one cell type to another. (pharmacy180.com)

Competition assays provide evidence that DNA strand separation and RNA-DNA heteroduplex formation initiate at the PAM and proceed directionally towards the distal end of the target sequence. (nature.com)
Here, we developed a platform with engineered crRNAs and optimized conditions that enabled us to detect DNA, DNA/RNA heteroduplex and methylated DNA with higher sensitivity, achieving a limit of detection of in femtomolar range without any target pre-amplification step. (biorxiv.org)

Class 2 CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated proteins) systems, such as Cas12a (Cpf1, subtype V-A) and Cas13a (C2c2, subtype VI), are capable of nonspecific cleavage of ssDNA (single-stranded DNA) and RNA, respectively, in addition to successful gene editing. (biorxiv.org)
We speculate that chemical modifications of the crRNA can potentially change its nature of binding and subsequently alter this collateral cleavage due to conformational changes of the Cas12a dynamic endonuclease domain. (biorxiv.org)
It accomplishes this cleavage by accelerating the hydrolysis of the phosphodiester links in the target DNA. (vedantu.com)
More recently, his group invented tools for cleavage-free large gene insertion via mining microbial recombination protein, and developed single-cell tracking approach for studying cancer biology and cancer immunology. (stanford.edu)
In either case, cleavage with the endonuclease results in fragments of lengths differing from the normal that can be detected by DNA hybridization. (pharmacy180.com)

The Non-specific nuclease, Benzonase - Endonuclease S. marcescens, has no proteolytic activity and is perfect for the removal of nucleic acids from protein samples or as an effective sonication alternative. (pharma-industry-review.com)
Serratia marcescens nuclease has potent digestive activity toward both single and double-stranded DNA and RNA of any origin. (pharma-industry-review.com)
Endonuclease G (ENDOG) is a nuclear-encoded mitochondrial-localized nuclease. (mdpi.com)
Specific functional analogies are drawn between several components of CASS and proteins involved in eukaryotic RNAi, including the double-stranded RNA-specific helicase-nuclease (dicer), the endonuclease cleaving target mRNAs (slicer), and the RNA-dependent RNA polymerase. (biomedcentral.com)
siRNAs are produced from double-stranded RNAs of viruses and transposable elements, which are processed by the dicer nuclease, one of the essential components of the RNA-Induced Silencing Complexes (RISCs) [ 7 - 9 ]. (biomedcentral.com)

The issues are particularly critical in precise targeted insertion of external DNA sequences into genomes. (molcells.org)
Southern blotting is a technique that can be used to detect specific sequences present in DNA. (pharmacy180.com)
The method can be used to amplify DNA sequences from any source. (pharmacy180.com)
Applications of the PCR technique include: 1) efficient comparison of a normal gene with a mutant form of the gene, 2) detection of low-abundance nucleic acid sequences, 3) forensic analysis of DNA samples, and 4) prenatal diagnosis and carrier detection (for example, of cystic fibrosis). (pharmacy180.com)
Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
All archaeal and many bacterial genomes contain Clustered Regularly Interspaced Short Palindrome Repeats (CRISPR) and variable arrays of the CRISPR-associated ( cas ) genes that have been previously implicated in a novel form of DNA repair on the basis of comparative analysis of their protein product sequences. (biomedcentral.com)

By the year 2000, RNA interference (RNAi) technology had emerged as a fast, simple, and inexpensive technique for targeted gene knockdown, and was routinely being used to study in vivo gene function in C. elegans. (wikipedia.org)
1990), fungi (quelling) (Romano and Marcino, 1992), and nematodes (RNA interference) (Fire et al. (inventiv.org)
1990), fungi (quelling) (Romano and Marcino 1992), and nematodes (RNA interference). (inventiv.org)
These 'guide' crRNAs are incorporated into a ribonucleoprotein interference complex that can recognize and cleave its complementary target sequence, thus destroying the invading nucleic acid. (elifesciences.org)

Most genetic diseases in humans are caused by point mutations-single base errors in the DNA sequence. (pharmaceuticalintelligence.com)
In DNA a sequence of three bases, which is called a codon, is responsible for the encoding of a single amino acid. (wikibooks.org)
Antiparallel and parallel motifs are found in the beta-sheets, which help in strand scission and sequence identification in the helices. (vedantu.com)
The human genome contains many thousands of polymorphisms (DNA sequence variations at a given locus). (pharmacy180.com)
The polymerase chain reaction (PCR), another method for amplifying a selected DNA sequence, does not rely on the biologic cloning method. (pharmacy180.com)
This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)
The nuclear localization sequence (nls) of His4 was introduced into the N-terminal of Streptococcus pyogenes (SpCas9) to target the nucleus of the endonuclease SpCas9. (biomedcentral.com)

Application areas include degradation of DNA after cell lysis, viscosity reduction of samples caused by HMW nucleic acids, DNA and RNA removal from protein samples, replacement of sonication or another mechanical DNA shearing process, as well as the prevention of cell clumping. (pharma-industry-review.com)
DNA is a long chain of linear polymers containing deoxyribose sugars and their covalently bonded bases known as nucleic acids. (wikibooks.org)
This phenomenon has been efficiently harnessed by SHERLOCK (Specific High-sensitivity Enzymatic Reporter unLOCKing) and DETECTR (DNA Endonuclease Targeted CRISPR Trans Reporter) to reliably detect nucleic acids. (biorxiv.org)

The SpCas9 expression and sgRNA (specific to eyfp ) production, which were controlled by His4 and EtU6 promoter, were constructed in one plasmid. (biomedcentral.com)

The CRISPR/Cas12a RNA-guided complexes have a tremendous potential for nucleic acid detection due to its ability to indiscriminately cleave ssDNA once bound to a target DNA. (biorxiv.org)

One of the major functions of the DNA is storage of the genetic information. (wikibooks.org)
It is significant to note the reasons why DNA is the primary method through which all cells pass along genetic information. (wikibooks.org)
Target-specific genetic modification technology is expected to have a leading position in future gene therapy development, and has a ripple effect on various basic and applied studies. (molcells.org)
For genome correction by introducing target-specific genetic scissors in various organisms, it is necessary to harness the endogenous cellular DNA repair system. (molcells.org)

Additionally, because RNAi reduces gene expression at the post-transcriptional level by targeting RNA, RNAi-based screens only result in partial and short-term suppression of genes. (wikipedia.org)

With virtually no proteolytic activity this Non-specific Endonuclease is an excellent tool for removal of nucleic acid contamination from purified proteins, commonly from recombinant DNA products, and reduction of viscosity of bacterial lysates for the improvement of downstream processed to mitigate protein degradation during drug manufacturing. (pharma-industry-review.com)
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. (nature.com)
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. (nature.com)
Bacterial DNA ligases can join two DNA fragments from different sources if they have been cut by the same restriction endonuclease. (pharmacy180.com)

DsDNA is a molecule that consists of two complementary strands of DNA that are bound together by hydrogen bonds. (inventiv.org)

Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller. (neb.com)
Encapsulation or formulation can protect siRNA from endonuclease degradation and enhance cellular uptake while promoting endosomal escape. (bvsalud.org)

Targeted approaches for gene knockdown emerged in the 1980s with techniques such as homologous recombination, trans-cleaving ribozymes, and antisense technologies. (wikipedia.org)
Subsequent repair of the DSBs is carried out by the host cell through either non-homologous end-joining pathway (NHEJ), leading to insertion and deletion mutations in the targeted genes, or homologous direct repair (HDR) in the presence of a DNA donor template. (biomedcentral.com)

The discovery of the elaborate and versatile systems of RNA silencing in eukaryotes is one of the pivotal advances in biology of the last decade [ 1 - 6 ]. (biomedcentral.com)

This hybrid combination of two fragments is called a recombinant DNA molecule. (pharmacy180.com)

The X-ray structure of the casposase bound to DNA representing the product of integration reveals a tetramer with target DNA bound snugly between two dimers in which single-stranded casposon end binding resembles that of spacer 3'-overhangs. (elifesciences.org)

The invention is more specifically directed at compositions that contain double-stranded DNA (?dsRNA? (inventiv.org)
The invention is directed to compositions that contain double stranded RNA (?dsRNA? (inventiv.org)
Suppression of gene expression by double-stranded RNA (dsRNA) has been demonstrated in a variety of systems including plants (post-transcriptional gene suppression) (Napoli et al. (inventiv.org)
1998). Double-stranded (dsRNA), which is more stable than ssRNA, has been shown to suppress gene expression in a variety of systems including plants (post-transcriptional gene suppression) (Napoli et al. (inventiv.org)

Although its precise biological function remains unclear, its proximity to mitochondrial DNA (mtDNA) makes it an excellent candidate to participate in mtDNA replication, metabolism and maintenance. (mdpi.com)
Indeed, several roles for ENDOG have been hypothesized, including maturation of RNA primers during mtDNA replication, splicing of polycistronic transcripts and mtDNA repair. (mdpi.com)
Protective methylation at the specified target site nucleotides, which is put in place immediately after replication, ensures that the bacteria's DNA remains untouched. (vedantu.com)

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. (nature.com)

DNA is more chemically stable than RNA because it lacks the hydroxyl group on the 2' carbon. (wikibooks.org)
9 , 10 In addition, chemically modified Cas12a guided-RNA has also been shown to facilitate improved gene correction in mammalian cells through both viral-and non-viral methods compared to the wild-type guide RNA. (biorxiv.org)

The CRISPR locus is then transcribed as a long CRISPR RNA (pre-crRNA) that is digested into shorter pieces (crRNAs). (elifesciences.org)

Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. (spandidos-publications.com)
Genes are small blocks of DNA that tell the cell which proteins it should create. (wikibooks.org)
One of the most promising approaches is the use of double-stranded DNA (dsDNA) to specifically target and inhibit the expression of genes. (inventiv.org)
This structure allows dsDNA to bind to specific regions of the genome, which can be used to target and inhibit the expression of specific genes. (inventiv.org)
Small interfering RNA (siRNA) is a potential method of gene silencing to target specific genes. (bvsalud.org)

Small CRISPR RNAs guide antiviral defense in prokaryotes. (nature.com)

With isothermal amplification of SARS-CoV-2 RNA using RT-LAMP, the modified crRNAs were incorporated in a paper-based lateral flow assay that could detect the target with up to 23-fold higher sensitivity within 40-60 minutes. (biorxiv.org)

In addition to the technical limitations that stem from the stochastic nature of DNA repair events, it is difficult to regulate programmable nucleases inside living systems. (molcells.org)

The bases of each strand bind very specifically, A binds with T and C binds with G no other combination exists at least in DNA. (wikibooks.org)
It only has one alpha subunit that binds to one DNA strand. (vedantu.com)
Surprisingly, we found that PRORP alone binds pre-tRNAs with nanomolar affinity and can even cleave some of them at reduced efficiency without the other subunits. (bvsalud.org)

When DNA is not self-replicating it exists in the cell as a double stranded helical molecule with the strands lined up anti-parallel to each other. (wikibooks.org)
In RNA there are two possible OH groups that the molecule can form a phosphodiester bond between, which means that RNA is not forced into the same rigid structure as its deoxy counterpart. (wikibooks.org)
Introduction of a foreign DNA molecule into a replicating cell permits the amplification (production of many copies) of the DNA, a process called cloning. (pharmacy180.com)
A vector is a molecule of DNA to which the fragment of DNA to be cloned is joined. (pharmacy180.com)

RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). (spandidos-publications.com)

Sending this machinery into cells creates a mismatched pair at the target, comprising the newly introduced U, and an original guanine base (G) on the opposite strand. (pharmaceuticalintelligence.com)
The same platform was used to detect as low as ~700 fM cDNA from HIV, 290 fM RNA from HCV, and 370 fM cDNA from SARS-CoV-2, all within 30 minutes without a need for target amplification. (biorxiv.org)
The discovery and mechanistic understanding of target-specific genome engineering technologies has led to extremely effective and specific genome editing in higher organisms. (molcells.org)
In particular, the use of the CRISPR system enabled convenient programming and manipulation of the genome at specific target loci. (molcells.org)
In this minireview, we describe recent efforts made to overcome these problems by using target-specific endonucleases, including CRISPR. (molcells.org)
Probes can be used to identify which clone of a library or which band on a gel contains the target DNA. (pharmacy180.com)

Next, we explain recently reported genome engineering methods for efficient knock-in of exogenous DNA, and we discuss future applications of genome editing technology. (molcells.org)

It is the absence of this group that allows DNA to form its conventional double helix structure. (wikibooks.org)

The market for methods, compositions, and compounds for the specific inhibition by double-stranded DNA of gene expression is a rapidly growing field in the biotechnology industry. (inventiv.org)
The market for methods, compositions, and compounds for the specific inhibition by dsDNA of gene expression is driven by the increasing demand for new and effective treatments for diseases that are caused by abnormal gene expression. (inventiv.org)
Several companies are currently developing methods and compounds for the specific inhibition by dsDNA of gene expression. (inventiv.org)

A DNA library is a collection of cloned restriction fragments of the DNA of an organism. (pharmacy180.com)

Researchers develop a CRISPR-based technique that efficiently corrects point mutations without cleaving DNA. (pharmaceuticalintelligence.com)

The absence of this Hydroxyl group is fundamental in determining the way in which DNA is able to condense itself within the nucleus of a cell. (wikibooks.org)

Each of the four bases on DNA, Adenine (A), Cytosine (C), Guanine (G), and Thymine (T) is bonded covalently to a deoxyribose sugar. (wikibooks.org)

Polymorphisms can arise from single-base changes and from tandem repeats. (pharmacy180.com)

However, the current CRISPR/Cas12a systems are limited to detecting DNA in a picomolar detection limit without an amplification step. (biorxiv.org)

A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)

Anatomy3

Organisms8

Chemicals and Drugs59

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes50

Information Science3

Health Care2

Enzyme19

Cas920

Nucleotide8

Enzymes7

Genomic3

Molecules3

Heteroduplex2

Cleavage5

Nuclease5

Sequences6

Interference4

Sequence8

Nucleic acids3

SgRNA1

Complexes1

Genetic4

RNAi1

Bacterial4

Complementary1

Degradation2

Homologous2

Eukaryotes1

Recombinant1

Bound1

DsRNA4

Replication3

Maturation1

Chemically2

Locus1

Genes5

Prokaryotes1

Sensitivity1

Programmable1

Binds3

Molecule4

Sanger1

Target6

Genome editing te1

Conventional1

Gene expression3

Fragments1

Mutations1

Nucleus1

Cytosine1

Repeats1

Amplification1

Polymorphisms1