Cleave the rna. Medical search. Frequent questions

RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)
However, a perceptive and hard-working graduate student, Ben Stark, noticed that an RNA copurified with the protein in the enzyme preparation. (nobelprize.org)
He then devised a test to see if the RNA molecule was essential for the function of the enzyme. (nobelprize.org)
We soon suggested that the RNA subunit of RNase P was part of the active center of the enzyme, by analogy to the then current picture of the ribosome. (nobelprize.org)
The book is thus essential reading for all molecular biologists and biochemists, as well as chemists interested in RNA technology, information storage, or enzyme catalysis. (cshlpress.com)
This gene provides instructions for making an enzyme that functions as an RNA exonuclease. (medlineplus.gov)
Specifically, the USB1 enzyme protects a small RNA molecule called U6, which helps in the assembly of the blueprints for protein production. (medlineplus.gov)
They can self-splice without the help of any proteins, whereas nuclear introns are spliced by the spliceosome, an RNA and protein-containing enzyme complex. (jove.com)
The term ribozyme is used for RNA that can act as an enzyme. (jove.com)
Class 2 type VI CRISPR-Cas13 systems use a single enzyme to target RNA using a programmable crRNA guide and are divided into four subtypes based on the identity of the Cas13 protein (Cas13a-d). (nih.gov)
The CRISPR Cas9 tool relies on a single, unique guide RNA that directs the Cas9 enzyme to bind with and cleave the corresponding targeted site in the genome. (ornl.gov)
The way electrons are distributed in the molecule influences reactivity and conformational stability, including the likelihood that the Cas9 enzyme-guide RNA complex will effectively bind with the microbe's DNA, said Erica Prates, computational systems biologist at ORNL. (ornl.gov)
An enzyme (protein) that's part of the human immunodeficiency virus reads the sequence of viral RNA nucleic acids (yellow in graphic) that have entered the host cell and transcribes the sequence into a complementary DNA sequence (shown in blue). (cellsalive.com)
Hepatitis C virus NS3-4A protease regulates the lipid environment for RNA replication by cleaving host enzyme 24-dehydrocholesterol reductase. (harvard.edu)
The Cas enzyme recognizes DNA molecules that contain non-self genetic information, e.g. from bacteriophages , and cleaves them at specific sites. (phys.org)
Together, the enzyme and the profile RNA constitute the CRISPR-Cas system. (phys.org)
If this kind of enzyme is specifically equipped with new RNA "profiles," it cleaves the cell's genome at precisely defined sites. (phys.org)
Using a multi-round selection process, the McMaster team uncovered an artificial DNA-RNA hybrid probe that is a substrate for a salmonella-specific form of an RNase H enzyme. (labmanager.com)

Zamore, P. D., Tuschl, T., Sharp, P. A. & Bartel, D. P. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. (nature.com)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
Our proprietary technology is "RNA switchTM", which utilizes the ability of microRNA to cleave mRNA. (bio.org)
RNA switch can have multiple microRNA binding sites tandemly and make programmable mRNA therapy such as NOT/OR conditions by designing the microRNA binding sites. (bio.org)
Messenger RNA (mRNA) molecules are encoded by genes and are themselves templates for the proteins that carry the main metabolic functions in a cell. (sciencedaily.com)
Within the complex, microRNAs are thought to bind to mRNA molecules containing a complementary stretch of RNA sequence. (sciencedaily.com)
The complex then cleaves the mRNA into smaller pieces, thereby preventing translation of the protein it encodes, and thus inhibiting or "silencing" gene expression. (sciencedaily.com)
miRNAs normally cleave mRNA by base-pairing to the 3′-untranslated region (UTR) of the target genes ( 9 ). (spandidos-publications.com)

a) Illustration of Dz13 cleaving its proper fluorescently labeled RNA substrate. (nature.com)
Here we present cryogenic electron microscopy structures of the Arabidopsis thaliana Argonaute10 (AtAgo10)-guide RNA complex with and without a target RNA representing a slicing substrate. (nature.com)
The leadzyme refers to a small ribozyme that cleaves a RNA substrate in the presence of Pb2+. (uwaterloo.ca)
MicroRNAs are ~22 nucleotide long RNA molecules that provide substrate specificity to a protein complex known as the RNA-induced silencing complex. (sciencedaily.com)
Within the cleft, at least five different subsites interact with the substrate, each subsite binding one monomeric unit of the polysaccharide. (lu.se)

Like protein enzymes, RNA enzymes (ribozymes) can catalyze (start or accelerate) chemical reactions that are critical for life. (wikipedia.org)
Protein enzymes may have come to replace RNA-based ribozymes as biocatalysts because their greater abundance and diversity of monomers makes them more versatile. (wikipedia.org)
One of the challenges in studying abiogenesis is that the system of reproduction and metabolism utilized by all extant life involves three distinct types of interdependent macromolecules (DNA, RNA, and protein). (wikipedia.org)
Gilbert pointed out that neither DNA nor protein were required in such a primitive system if RNA could perform as a catalyst. (nobelprize.org)
Ensuing discoveries of other natural catalytic RNAs that could cleave and ligate phosphodiester bonds, and the very recent observation that the region surrounding the peptidyl transferase center of a bacterial 50S ribosomal subunit contains RNA and no protein, further buttress the hypothesis. (nobelprize.org)
The non-structural protein NSP2 of rotavirus, which exhibits nucleoside triphosphatase, single-stranded RNA binding, and nucleic-acid helix-destabilizing activities, is a major component of viral replicase complexes. (nih.gov)
Every bacterium hosts inside itself a combination of a toxin (T), usually a protein, and an antitoxin (A), which can be a protein or an RNA molecule. (thehindubusinessline.com)
Of the eight types of TA systems identified so far, Singh's lab is interested in the Type III TA system, where the antitoxin is not a protein but rather a stretch of ribonucleic acid (RNA). (thehindubusinessline.com)
The toxin gene gets transcribed on to the RNA which then is used to produce the toxin protein, but the antitoxin gene only gets transcribed to RNA. (thehindubusinessline.com)
Being the RNA, it gets degraded faster than the protein toxin. (thehindubusinessline.com)
Simply put, Singh's team has helped establish the structure and binding nature between the antitoxin RNA and the toxin protein. (thehindubusinessline.com)
Integrator and protein phosphatase 2A (PP2A) form a complex that dephosphorylates paused RNA polymerase II (Pol II), cleaves the nascent RNA, and terminates transcription. (rcsb.org)
May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin (By similarity). (antikoerper-online.de)
A second report focuses on the function of the protein ARGONAUTE1 (AGO1), a major component of the RNA-induced silencing complex in Arabidopsis. (sciencedaily.com)
MRRF controls the release of ribosome from messenger RNA at the termination of protein biosynthesis. (prospecbio.com)
We have these molecules that try to block a protein from its receptor, prevent a protein from cleaving another protein, or increase the rate that a protein is broken down. (medscape.com)
As the messenger RNAs are transcribed and then translated the protein Cas9 in complex with the gRNA returns to the plasmid and creates DSB, effectively disrupting its function and destroying the delivery mechanism," the researchers write. (genomeweb.com)
PRKRA, a protein kinase, interferon-inducible double stranded RNA dependent activator protein kinase, also known as PACT, a protein activator of the interferon-induced protein kinase, is a product of PRKRA gene [ 1 , 2 ] and is a member of the dsRNA binding protein family [ 3 ]. (hindawi.com)
PRKRA is contained in the cytoplasmic RNA-induced silencing complex (RISC) together with transactivation response RNA binding protein (TRBP) and Dicer [ 4 - 6 ]. (hindawi.com)

The RNA world is a hypothetical stage in the evolutionary history of life on Earth, in which self-replicating RNA molecules proliferated before the evolution of DNA and proteins. (wikipedia.org)
In an article he contributed to a volume issued in honor of Nobel-laureate physiologist Albert Szent-Györgyi, he explained that the primitive Earth's environment could have produced RNA molecules (polynucleotide monomers) that eventually acquired enzymatic and self-replicating functions. (wikipedia.org)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
Transfer RNA (tRNA) molecules contain various post-transcriptional modifications that are crucial for tRNA stability, translation efficiency, and fidelity. (mdpi.com)
They preferentially cleave single-stranded RNA molecules between purine and uridine residues to generate two nucleotide fragments with 2'3'-cyclic phosphate adenosine/guanosine terminus and uridine residue, respectively. (frontiersin.org)
Ribonucleases (RNases) are RNA-processing or -degrading enzymes that hydrolyze phosphodiester bonds within RNA molecules ( 1 ). (frontiersin.org)
RNA exonucleases cut off (cleave) building blocks called nucleotides one at a time from molecules of RNA (a chemical cousin of DNA). (medlineplus.gov)
however, Ribonuclease P can cleave other RNA molecules. (jove.com)
MicroRNAs are tiny ribonucleic acid (RNA) molecules (~22 nucleotides long) that recently have been found to play important roles in regulating gene expression in eukaryotic organisms, including plants and animals. (sciencedaily.com)
They turned to quantum biology, a field bridging molecular biology and quantum chemistry that investigates the effects that electronic structure can have on the chemical properties and interactions of nucleotides, the molecules that form the building blocks of DNA and RNA. (ornl.gov)
MicroRNAs (miRNAs) are small non-coding RNA molecules (containing ~22 nucleotides) that function in RNA silencing and post-transcriptional regulation of gene expression. (spandidos-publications.com)

Further concept of RNA as a primordial molecule can be found in papers by Francis Crick and Leslie Orgel, as well as in Carl Woese's 1967 book The Genetic Code. (wikipedia.org)
One version of the hypothesis is that a different type of nucleic acid, termed pre-RNA, was the first one to emerge as a self-reproducing molecule, to be replaced by RNA only later. (wikipedia.org)
Only upon the presence of an appropriate input molecule, the two parts are joined, the DNAzyme complex is formed and the cleavage of RNA occurs ('True' output) ( Fig. 1b ). (nature.com)
At that time, Tom Cech had recently and independently observed phosphoester bond cleavage and ligation by a different RNA molecule. (nobelprize.org)
Once thought to be just a messenger that allows genetic information encoded in DNA to direct the formation of proteins, RNA (ribonucleic acid) is now known to be a highly versatile molecule that has multiple roles in cells. (cshlpress.com)
Subsequent chapters examine riboswitches and ribozymes, establishing what the RNA molecule is capable of alone. (cshlpress.com)
Following tissue damage, RNase T2 is secreted and participates in resistance against RNA viruses or functions as an alarm signaling molecule to regulate the host immune response and contributes to tissue remodeling and repair ( 12 , 13 ). (frontiersin.org)

This defense is based on a common mechanism, a CRISPR ribonucleic acid (crRNA), a "guide RNA" that helps detect regions of a foreign genome, such as the DNA of a virus, for targeted cleavage. (isaaa.org)

If the RNA world existed, it was probably followed by an age characterized by the evolution of ribonucleoproteins (RNP world), which in turn ushered in the era of DNA and longer proteins. (wikipedia.org)
Argonaute (AGO) proteins use small RNAs to recognize transcripts targeted for silencing in plants and animals. (nature.com)
During this stage, proteins were not yet engaged in biochemical reactions and RNA carried out both the information storage task of genetic information and the full range of catalytic roles necessary in a very primitive self-replicating system. (nobelprize.org)
CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) adaptive immune systems defend microbes against foreign nucleic acids via RNA-guided endonucleases. (nih.gov)
These systems are divided into two classes: class 1 systems utilize multiple Cas proteins and CRISPR RNA (crRNA) to form an effector complex while class 2 systems employ a large, single effector with crRNA to mediate interference. (nih.gov)
The Cas13 proteins are capable of both pre-crRNA processing and target RNA cleavage, which protect the host from phage attacks. (nih.gov)
There are many AGO1-like proteins in animals and other eukaryotes as well, indicating that the RNA-induced silencing complex is of ancient evolutionary origin, and that microRNA-mediated regulation of gene expression is shared among many eukaryotes. (sciencedaily.com)
Even viruses can't replicate if their RNA is prevented from being turned into proteins. (medscape.com)
The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including PRKRA, TRBP, and Dicer. (hindawi.com)

Suggestions for 'simple' pre-RNA nucleic acids have included peptide nucleic acid (PNA), threose nucleic acid (TNA) or glycol nucleic acid (GNA). (wikipedia.org)
Despite their structural simplicity and possession of properties comparable with RNA, the chemically plausible generation of "simpler" nucleic acids under prebiotic conditions has yet to be demonstrated. (wikipedia.org)
They trained the model on a dataset of around 50,000 guide RNAs targeting the genome of E. coli bacteria while also taking into account quantum chemical properties, in an approach described in the journal Nucleic Acids Research . (ornl.gov)
Other defenses include Argonaute-based systems that utilize both DNA and RNA guides to destroy foreign nucleic acids and abortive infection (Abi), which is a measure of last resort, such that death of the infected cells prevents spread of infection. (benthamscience.com)

At that time, it had only been demonstrated that RNA could cleave or ligate phosphodiester bonds. (nobelprize.org)
The substrates for most naturally occurring ribozymes are RNA phosphodiester bonds. (jove.com)

Also covered are viral RNAs, telomerase RNA, and tools for scientists who work on RNA. (cshlpress.com)
The replication process in these viruses produce long RNA carrying multiple units of the viral genome where each unit carries a small ribozymes like hammerhead. (jove.com)
CRISPR-Cas systems enable bacteria to acquire immunity against their viruses by capturing snippets of their DNA and using RNA-guided nucleases that cleave the viral DNA. (rockefeller.edu)
These include AZT, 3TC, d4T, ddc, and ddl that act by blocking the recoding of viral RNA into DNA. (cellsalive.com)
In particular, the RNA interference (RNAi) pathways are of notable significance for the control of viral infections. (mdpi.com)
Viral RNA obtained from a bronchoalveolar lavage specimen from the patient has been sequenced and genetically analyzed by the National Influenza Centre in Chile (Instituto de Salud Pública) and by the Influenza Division/CDC. (cdc.gov)

Severe scarring is one of the most serious complications after cleft lip and palate surgeries. (nova.edu)
Overall, 20 of 23 sporadic patients with a COL2A1 mutation had either a cleft palate or retinal detachment with vitreous anomalies. (nih.gov)
The presence of vitreous anomalies, retinal tears or detachments, cleft palate and a positive family history were shown to be good indicators for a COL2A1 defect. (nih.gov)

87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)

In addition, mitochondrial DNA (mtDNA) encodes 2 ribosomal RNA genes and 22 transfer RNA (tRNA) genes necessary for the intramitochondrial synthesis of these 13 polypeptides. (medscape.com)
My involvement in the discovery of the first catalytic RNA began in innocence during a study of tRNA biosynthesis in Escherichia coli. (nobelprize.org)
Its function was to cleave a phosphodiester bond at the start of the mature tRNA nucleotide sequence, thereby releasing the upstream extra or "precursor" nucleotides. (nobelprize.org)
Besides their canonical roles in translation, tRNAs also originate tRNA-derived small RNAs (tsRNAs), a class of small non-coding RNAs with regulatory functions ranging from translation regulation to gene expression control and cellular stress response. (mdpi.com)

Kikkert M . Innate immune evasion by human respiratory RNA viruses. (cdc.gov)
These can be found in many RNA plant viruses, as well as the hepatitis delta virus, a human pathogen. (jove.com)
Retroviruses are enveloped RNA viruses defined by their mechanism of replication via reverse transcription to produce DNA copies that integrate into the host cell's genome. (msdmanuals.com)

Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes. (nature.com)
RNA cleavage is promoted by one to three histidine residues that are located in CAS I and II. (frontiersin.org)
These regularly spaced hammerhead enzymes undergo self-cleavage resulting in breakage of the long RNA into individual genome segments. (jove.com)
There are two distinct types of Cas13a enzymes, based on their cleavage preference: adenosine (A) cleaving or uridine (U) cleaving. (nih.gov)

The phrase "RNA World" was first used by Nobel laureate Walter Gilbert in 1986, in a commentary on how recent observations of the catalytic properties of various forms of RNA fit with this hypothesis. (wikipedia.org)
The phrase "The RNA World" was coined by Walter Gilbert in 1986 in a commentary on the then recent observations of the catalytic properties of various RNAs. (nobelprize.org)
Finally, the so-called "evolution in vitro " methodology, which is able to scan an enormous number of nucleic acid sequences in vitro for any given function, has revealed that RNA, indeed, can have many different catalytic functions as so can, presumably, DNA. (nobelprize.org)
On further reflection, many doubts have been raised about whether or not the original genetic/catalytic material could have been RNA as we know it today because extreme conditions on the primitive Earth might have led to the rapid chemical degradation of RNA. (nobelprize.org)
Nevertheless, even if the precise chemical nature of the early genetic/catalytic material differed from present-day RNA, it seems reasonable to conclude that the RNA World did exist at some time. (nobelprize.org)
Cech's observation and ours, while still greeted skeptically by some members of the enzymological community, were soon universally accepted and within a few years other catalytic RNAs derived from plant pathogens and the human delta RNA were also found. (nobelprize.org)

This structural similarity suggests that the nucleotide-binding site is located inside the cleft between the two domains. (nih.gov)
Small ribozymes are usually 50 to 150 nucleotides long and are self-cleaving nucleotide sequence motifs. (jove.com)

MRRF attaches to the large ribosomal subunit in the cleft which has a peptidyl transferase center. (prospecbio.com)

Most RNA-cleaving DNAzymes are much longer. (uwaterloo.ca)
RNA-cleaving DNAzymes provide a unique platform for developing biosensors. (uwaterloo.ca)
Two RNA-cleaving DNAzymes (17E and 10-23) are the most widely used. (uwaterloo.ca)

One of the most critical components of cells, the ribosome, is composed primarily of RNA. (wikipedia.org)
The only known exception to this is the 23S ribosomal RNA in the bacterial ribosome which catalyzes peptide bond formation. (jove.com)

2) An (RNA) containing uridine + H(2)O = an (RNA)-3'-uridine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA). (cathdb.info)

Ribozymes are special types of RNA that can act as enzymes. (jove.com)

Plant small RNAs: advancement in the understanding of biogenesis and role in plant development. (nature.com)

Several RNA binding sites, resulting from the quaternary organization of NSP2 monomers, may be required for the helix destabilizing activity of NSP2 and its function during genome replication and packaging. (nih.gov)

The main biological roles of these ribonucleases are cleaving or degrading RNA substrates. (frontiersin.org)

Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
The intracellular distribution pattern suggests that RNase T2 may be involved in degrading exogenous or endogenous RNAs in lysosome and regulating mitochondrial RNA metabolism ( 6 - 8 ). (frontiersin.org)

Spacer sequences are transcribed into CRISPR RNAs (crRNAs)-small RNAs that, through base-pairing interactions with the target sequence, guide Cas nucleases to the invasive nucleic acid to cleave it. (rockefeller.edu)
Reverse transcriptase sometimes makes mistakes reading the RNA sequence. (cellsalive.com)

Considering how well different nucleases have been translated into new and improved technologies, any discovery in this field could bring new benefits to society," says Chase Beisel from the Würzburg Helmholtz Institute for RNA-based Infection Research (HIRI). (isaaa.org)
Oleg Dmytrenko, the first author of the study, said that they were exploring CRISPR nucleases that were originally clumped with Cas12a, nucleases that defend bacteria by recognizing and cleaving invasive DNA. (isaaa.org)
After a pathogen enters the cell, it can be destroyed by sugar-nonspecific nucleases that cleave both DNA and RNA. (benthamscience.com)

This free toxin cleaves the phage RNA (along with the bacterial RNAs), effectively preventing phage propagation from infecting other bacteria. (thehindubusinessline.com)

The book goes on to discuss the evolution of ribosomes and the functions of RNPs, before reviewing the recent work that has revolutionized our understanding of gene regulation by non-coding RNAs, including miRNAs and siRNAs. (cshlpress.com)

Although RNA is fragile, some ancient RNAs may have evolved the ability to methylate other RNAs to protect them. (wikipedia.org)

P.272-280.Mitkevich V.A, Tchurikov N.A, Zelenikhin P.V, Binase cleaves cellular noncoding RNAs and affects coding mRNAs//FEBS Journal. (kpfu.ru)
Dmytrenko added that a CRISPR-based defense mechanism that relies on a single nuclease to recognize the invader and degrade cellular DNA and RNA has not been observed before. (isaaa.org)
This copy, a kind of " molecular profile " of bacteriophage DNA and other foreign genetic material , exists as RNA, an important cellular building block, which is used, among other things, as a temporary storage site of genetic information . (phys.org)

The proposed mechanism is based on the knowledge that transcription of mtDNA is polycistronic, which means that all genes encoded on the heavy and light strands are transcribed as 2 large precursor RNA strands. (medscape.com)

Krzysztof Chylinski, Anaïs Le Rhun, Emmanuelle Charpentier, The tracrRNA and Cas9 families of type II CRISPR-Cas immunity systems, RNA Biology , 2013. (phys.org)
Their approach builds on the CRISPR-Cas9 system, but adds a self-cleaving mechanism that destroys the message. (genomeweb.com)

The RNA World referred to an hypothetical stage in the origin of life on Earth. (nobelprize.org)

Results show that prokaryotic and eukaryotic AGOs use distinct mechanisms for achieving target slicing and provide insights into small interfering RNA potency. (nature.com)
The model helped us identify clues about the molecular mechanisms that underpin the efficiency of our guide RNAs," Prates said, "giving us a rich library of molecular information that can help us improve CRISPR technology. (ornl.gov)

Importantly, DTh recognizes cancer marker RNA, while cleaving other targeted RNA, which offers a possibility to suppress vital genes exclusively in cancer cells thus triggering their death. (ifmo.ru)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. (spandidos-publications.com)

Slicing by DNA-guided prokaryotic AGOs has been studied in detail, but structural insights into RNA-guided slicing by eukaryotic AGOs are lacking. (nature.com)
Fig. 4: Structural basis for RNA slicing. (nature.com)

LOCUS AJ583672 4970 bp RNA linear VRL 26-JUL-2016 DEFINITION Norwalk virus gene for polyprotein (partial), ORF2 and ORF3, genomic RNA. (cdc.gov)

When Cas12a2 recognizes invasive RNA, the nuclease cleaves it but can also damage other RNA and DNA inside the cell. (isaaa.org)

When a phage attacks bacteria, it takes over the host's internal machinery to multiply its genetic material (DNA or RNA) inside, killing the bacteria in the process. (thehindubusinessline.com)

Methionine - involved in DNA/RNA chemistry. (michaelshealth.com)
In 2014, he joined the group of Dr. Jonathan Watts to pursue a PhD in chemistry at the University of Southampton (UK) and the RNA Therapeutics Institute (USA). (rsc.org)

The Integrator endonuclease docks to the RNA exit site and opens to cleave nascent RNA about 20 nucleotides from the Pol II active site. (rcsb.org)

Specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds in RNA. (cathdb.info)
A team of researchers led by Yingfu Li, Tohid Didar, and Carlos Filipe of McMaster University in Hamilton, Canada, have now developed a novel test system based on a hybrid DNA-RNA probe that specifically and rapidly detects salmonella, without the need for microbiological diagnostics or expensive analytical equipment. (labmanager.com)
However, when the sample mixture contains salmonella, the upper layer is released thanks to the salmonella RNase H specifically cleaving the DNA-RNA linker probe. (labmanager.com)

Regardless of its plausibility in a prebiotic scenario, the RNA world can serve as a model system for studying the origin of life. (wikipedia.org)
On the other hand, the discovery in 2009 that activated pyrimidine ribonucleotides can be synthesized under plausible prebiotic conditions suggests that it is premature to dismiss the RNA-first scenarios. (wikipedia.org)

Like DNA, RNA can store and replicate genetic information. (wikipedia.org)

Enter small inhibitory RNAs, or siRNAs for short, discovered in 1998 by Andrew Fire and Craig Mello at UMass Worcester. (medscape.com)

To improve the modeling and design of guide RNA, the ORNL scientists sought a better understanding of what's going on at the most basic level in cell nuclei, where genetic material is stored. (ornl.gov)

aRNA is RNA that is transcribed from the coding, rather than the template, strand of DNA. (genomicglossaries.com)
Endoribonucleases that cleave RNA at 5'-polyuridine locations within the RNA strand. (bvsalud.org)

Anatomy16

Organisms27

Diseases1

Chemicals and Drugs120

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes76

Information Science4

Health Care1

Enzyme18

MRNA11

Substrate5

Protein19

Molecules11

Molecule7

Ribonucleic acid1

Proteins9

Nucleic acids4

Phosphodiester bonds2

Viral6

Palate3

Polymerase2

TRNA4

Viruses3

Cleavage4

Catalytic6

Nucleotide2

Subunit1

DNAzymes3

Ribosome2

Uridine1

Ribozymes1

Biogenesis1

Genome1

Substrates1

Endogenous2

Sequence2

Nucleases3

Bacterial1

MiRNAs1

Methylate1

Cellular3

Transcription1

Cas92

Hypothetical stage1

Mechanisms2

Genes3

Structural2

Genomic1

Recognizes1

Phage1

Chemistry2

Endonuclease1

Specifically3

Prebiotic2

Replicate1

Small1

Genetic1

Strand2