Claudins expressed in cultured epithelial cell. Medical search. Frequent questions

Interestingly, the necessity of occludin to spermatogenesis doesn't apply to humans as occludin was not found in human Sertoli cells in an earlier study (Moroi et al. (flapinhibitor.com)
1998). Nonetheless, a current study by RT-PCR has identified occludin in human Sertoli cells (Xiao and Cheng, unpublished observations), illustrating additional study on the function of occludin in huamn BTB is warranted. (flapinhibitor.com)
It was reported that occludin was not located inside the TJ strands among porcine aortic endothelial cells (Hirase et al. (flapinhibitor.com)
Moreover, in occludin knockout mice, the TJ barrier formed between intestinal epithelial cells was indistinguishable from those of the wild sort ultrastructurally (Saitou et al. (flapinhibitor.com)
2000), demonstrating that in some epithelia that commonly express occludin, a missing of occludin doesn't necessarily impact the formation and/or maintenance of the TJ barrier. (flapinhibitor.com)
Moreover, while studies have shown that therapy of synthetic occludin peptide disrupted TJ barrier in between Sertoli cells (Chung et al. (flapinhibitor.com)
readily available in PMC 2014 July 08.Mok et al.Web page(T84) cell cultures has shown that the occludin peptide-induced TJ-barrier disruption was mediated by redistribution of other TJ proteins (e.g. claudin-1) and TJ adaptor (e.g. (flapinhibitor.com)
A lot more critical, the use of monoclonal antibody against the second extracellular loop of occludin in T84 cells was identified to disrupt epithelial cell polarity but not the TJ barrier (Tokunaga et al. (flapinhibitor.com)
Nonetheless, these findings illustrate that occludin, unlike claudins, may have other function(s) and ETB Source serving as a signaling molecule in controlling the permeability in TJs, such as fine-tuning the barrier function, in addition to serving as the developing block of TJs in some epithelia. (flapinhibitor.com)
The TJ consists of transmembrane proteins occludin, claudins, and junctional adhesion molecules (JAMs), which are organized in intramembranous strands and are linked to the F-actin cytoskeleton either directly or indirectly through members of the MAGUK (membrane-associated guanylate kinase) family of proteins ZO-1, -2, and -3. (rupress.org)
In this study, we investigated the effects of TNF-α and propofol on occludin expression in human cerebral microvascular endothelial cell line, D3 clone (hCMEC/D3 cells), and explored the underlying mechanisms. (researchsquare.com)
Cerebral microvascular endothelial cells are linked together through the interactions among multiple tight junction proteins including claudins, occludin and ZO-1 [ 5 ]. (researchsquare.com)
It has been reported that TNF-α could decrease the expression of occludin in endothelial cells [ 13 ]. (researchsquare.com)
The kit CSB-EL016263HU is used for cell lysates sample and for the quantitative determination of human occludin (OCLN). (cusabio.com)
There exsit the quantitiy difference between different cell type Occludin(OCLN). (cusabio.com)

Lung epithelial cells form a barrier that lines airway and alveolar surfaces and constitute an air-liquid interface. (springer.com)
For over 50 years, glucose has been recognised to cross the lung epithelial barrier and be transported by lung epithelial cells. (ersjournals.com)
Since the mid-1960s, it has been known that there are energy-dependent, sodium-coupled glucose transporter (SGLT) and energy-independent, facilitative glucose transporter (GLUT) pathways for glucose uptake in the lung [ 1 ], and that glucose can permeate the alveolar epithelial barrier [ 2 ]. (ersjournals.com)
Important for the barrier function of intestinal epithelium, its cells are joined securely together by four types of junctions (cell junctions), which can be identified at the ultrastructural level: Gap junctions Desmosomes Adherens junctions Tight junctions Gap junctions bring the adjacent cells within 2 nanometers of each other. (wikipedia.org)
Barrier functions of TJ were examined in wild-type or DUSP3-deficient lung epithelial cells. (biomedcentral.com)
Normal epithelial cells, particularly in simple epithelium, have apical and basal-lateral polarity and form barrier against environmental challenges. (biomedcentral.com)
On the other hand, TJs are specifically important for maintaining epithelial barrier because of their structure [ 4 ]. (biomedcentral.com)
A hydrogel model of the human blood-brain barrier using differentiated stem cells. (open.ac.uk)
An In Vitro Blood-Brain Barrier Model to Study Firm Shear Stress-Resistant Leukocyte Adhesion to Human Brain Endothelial Cells. (open.ac.uk)
Previous studies indicated that endotoxins usually caused intestinal mucosa inflammation and destruction of tight junction integrity and epithelial cell apoptosis [ 4 ], which subsequently led to disruption of intestinal homeostasis and damage of the intestinal barrier functions. (biomedcentral.com)
The intestinal epithelial barrier serves as the first line of defense between the host and the luminal environment. (mdpi.com)
Blood-brain barrier (BBB) is a multicellular structure and mainly consists of microvascular endothelial cells, astrocytes, pericytes, intercellular tight junctions and basal lamina [ 1 ]. (researchsquare.com)

It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)
Furthermore, the portion of CTCs that extravasate and are able to generate distant metastases may have lost EpCAM manifestation and are believed to have undergone the epithelial-mesenchymal transition (EMT), which results in the downregulation of epithelial cell markers, such as E-cadherin, claudins, cytokeratins, and EpCAM (10-13). (biotechnologyconsultinggroup.com)
During oncogenesis, cancer cells undergo epithelial-mesenchymal transition (EMT), lose their polarity, and gain higher migratory and invasive abilities. (biomedcentral.com)
In this pathological process, retinal pigment epithelial (RPE) loses epithelial characteristics through an epithelial-mesenchymal transition (EMT), transforms into mesenchymal phenotype, increasing cells migration ability, invasiveness, resistance to apoptosis, and production of extracellular matrix, turning RPE into fibroblast-like cells [ 7 ]. (ijbs.com)
Therefore, cellular stresses induced during cell isolation cause dedifferentiation and transition to duct-like cells through activation of p38 MAP kinase and constitutively active Src kinases. (sykinhibitors.com)

In epithelial cells, apical-basal polarity is maintained through the formation of several intercellular adhesion systems consisting of adherens junctions (AJs), desmosomes, and tight junctions (TJs). (rupress.org)
Cell adhesion molecule-1 (CADM1) is a member of the immunoglobulin superfamily that functions as a tumor suppressor of lung tumors. (cancerindex.org)
The protrusions of the cell edge are shown as green arrows corresponding to points of adhesion maturation (panels 3 and 4). (mechanobio.info)
Panel 4 shows sliding of adhesion and hence slight retraction of the respective section of the cell edge compared to panel 3. (mechanobio.info)
Background Snail is a transcriptional element which may impact the epitheliomesenchymal changeover (EMT) by regulating adhesion protein such as for example E-cadherin and claudins aswell seeing that matrix metalloproteases (MMP). (hiv-proteases.com)
It is able to induce adhesion when expressed in cells lacking tight junctions. (cusabio.com)

TJs, tightly fused regions between the outer membrane of adjacent cells, are formed by integral transmembrane proteins such as OCLN and claudins, anchoring proteins such as zonula occludens (ZO)1-3, and other TJ-associated/regulatory proteins [ 4 , 5 ]. (biomedcentral.com)
In this study, we investigated the expression of mesenchymal biomarkers in autophagy deficiency cells and found these proteins were increased. (ijbs.com)
The cytoskeleton is a highly dynamic network of filamentous proteins that enables the active transport of cellular cargo, transduces force, and when assembled into higher-order structures, forms the basis for motile cellular structures that promote cell movement. (mechanobio.info)

We asked regardless of whether the antiproliferative action of 17-AAG was as a consequence of cell cycle arrest, apoptosis, or each. (hsp90-inhibitors.com)
Annexin-V/PI movement cytometric experiments had been performed to determine if 17-AAG alone or in combination with cisplatin could induce A549 cell apoptosis. (hsp90-inhibitors.com)
The antibody is useful for studying the phenomenon of cell death by apoptosis as a pathogenic mechanism of numerous pathologies: nerve damage, neurodegenerative diseases, cardiovascular diseases, autoimmune diseases, AIDS and malignancies. (samatashkhis.com)

Overall, the data show that a double endothelial and epithelial check point controls the transchoroidal migration of neutrophils into the developing brain. (biomedcentral.com)
It has been reported that the BBB protects CNS from harmful molecules and pathogens in the blood, and the effects are based on the structure of tight junctions between cerebral microvascular endothelial cells [ 6 ]. (researchsquare.com)
In the skin, H1Rs are expressed on vascular smooth muscle cells, endothelial cells, neurons, and different immune cells such as monocytes, neutrophils, DCs, and T and B cells where their activation drives the typical symptoms of immediate hypersensitivity responses, comprising edema and pruritus [ 10 ]. (springeropen.com)

Many of the genes necessary for differentiation into the different epithelial cell types have been identified and characterized (see this table). (wikipedia.org)
Acute myeloid leukemia (AML) is a heterogenous hematological malignancy characterized by inhibited differentiation and uncontrolled proliferation of hematopoietic stem/progenitor cells (HSPC). (biomedcentral.com)
In the earliest stages of development, the physical properties of the microenvironment can direct cell differentiation, and initiate the coordinated movement of groups of cells to establish the patterns that will define how the body is arranged. (mechanobio.info)
In this study, we found that this change of claudin expression is a process of dedifferentiation, because expression of other differentiation markers also changes during culture. (sykinhibitors.com)
Explore our series of recombinant neural factors to support the culture and differentiation of nerve cells. (acrobiosystems.com)

The GTPases Rac and Cdc42 play a pivotal role in the establishment of cell polarity by stimulating biogenesis of tight junctions (TJs). (rupress.org)
In this study, we show that the Rac-specific guanine nucleotide exchange factor Tiam1 (T-lymphoma invasion and metastasis) controls the cell polarity of epidermal keratinocytes. (rupress.org)
Our data indicate that Tiam1-mediated activation of Rac in PAs controls TJ biogenesis and polarity in epithelial cells by association with and activation of the Par3-Par6-aPKC polarity complex. (rupress.org)

The intestinal epithelium is the single cell layer that form the luminal surface (lining) of both the small and large intestine (colon) of the gastrointestinal tract. (wikipedia.org)
To securely contain the contents of the intestinal lumen, the cells of the epithelial layer are joined together by tight junctions, thus forming a contiguous and relatively impermeable membrane. (wikipedia.org)
Renewal relies on proliferative cells (stem cells) that reside at the crypt (base) of the intestinal glands (epithelial invaginations into the underlying connective tissue). (wikipedia.org)
Subsets of sensory intestinal epithelial cells synapse with nerves, and are known as neuropod cells. (wikipedia.org)
2001) at the same time as that in between intestinal epithelial cells (Nusrat et al. (flapinhibitor.com)
2005), a study in human intestinal T84 epithelialNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptInt Rev Cell Mol Biol. (flapinhibitor.com)
It consists of a continuous monolayer of intestinal epithelial cells connected by intercellular junctional complexes which shrink the space between adjacent cells. (mdpi.com)

During embryonic stages of lung organogenesis, TGF-β1 is pivotal in epithelial-mesenchymal interaction, during alveolarization and for airway branching morphology. (springer.com)
In vitro studies in resistive human lung epithelial cell monolayers grown at an air-liquid interface have supported these in vivo observations. (ersjournals.com)
High levels of CLDN6 are associated with non-small-cell lung cancer. (cusabio.com)
This is in contrast to Ciproxifan the results of some studies indicating Ciproxifan induction of MMP9 or MMP2 by snail in tumors but this kind of effect may be cell line specific [9 24 However we could not observe any relation between snail and these MMPs in the array material with all results indicating that there was no evident association between MMP2 and 9 and snail in lung carcinoma. (hiv-proteases.com)
The nuclear tumor-cell-associated expression of snail was strongest in small cell carcinoma of the lung a tumor type which is one of the most aggressive lung tumors. (hiv-proteases.com)
On the other hand tumor-cell-associated nuclear snail expression was not found in carcinoid tumors of the lung also indicating that there is a link between such nuclear snail expression and biological behaviour of tumors. (hiv-proteases.com)

Cell membranes are highly enriched in signaling receptors, transmembrane mechanosensors, pumps and channels, and, depending on their makeup, can recruit and retain a pool of mechanosensors important in the field of mechanobiology. (mechanobio.info)

This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. (springer.com)
In summary, we demonstrate a role of motile cilia in TGF-β1 sensing and showed that TGF-β1 disturbs TJ permeability of conductive airway epithelia by redistributing CLDN3 from TJs into cell nuclei. (springer.com)
Glucose diffusion is determined by epithelial permeability to glucose, the transepithelial glucose gradient and surface area. (ersjournals.com)

In immortalised human airway cells (line H441) with 10 mM glucose in the basolateral medium, apical ASL glucose was 0.24±0.07 mM [ 10 ]. (ersjournals.com)

The expression of ASK1 is correlated with the level of claudin-6 in cervical carcinoma cells and tissues. (cusabio.com)
This work provides a proof of concept for the use of Claudin-6 to eliminate residual undifferentiated human pluripotent stem cells from culture. (cusabio.com)
Although claudin-6 and claudin-9 can serve as entry factors in cell lines, hepatitis C virus infection into human hepatocytes is not dependent on claudin-6 and claudin-9. (cusabio.com)
We postulate that the tight junction protein claudin-19 is expressed in IMCD and that it takes part in epithelial adaptation to changing osmolality at different functional states. (uni-luebeck.de)
IMCD cells cultivated at 300 mosm/kg showed high transepithelial resistance, a cation selective paracellular pathway and claudin-19 was mainly located in the tight junction. (uni-luebeck.de)
In contrast, IMCD cells cultivated at 900 mosm/kg had low transepithelial resistance, anion selectivity, and claudin-19 was relocated from the tight junctions to intracellular vesicles. (uni-luebeck.de)
Taken together with our knowledge of claudin-6 and -10 function in other vertebrates, data support the idea that in SW-acclimated T. nigroviridis , these claudins are abundant in gill ionocytes, where they contribute to the formation of a Na + shunt and 'leaky' epithelium, both of which are characteristic of salt-secreting SW fish gills. (silverchair.com)
In skin, claudin-6 was found to co-localize with NKA-ir cells, but claudin-10d and -10e did not. (silverchair.com)
Moreover, in autophagy deficient RPE cell line via knockdown autophagy related protein 7 (Atg7), the expression of epithelial marker claudin-1 was suppressed and the mesenchymal markers were increased, accompanied by an increase in cell migration and contractility. (ijbs.com)
Description: Quantitativesandwich ELISA kit for measuring Human Claudin-2(CLDN2) in samples from serum, plasma, tissue homogenates, cell lysates. (scalegen.com)
Description: A competitive ELISA for quantitative measurement of Human Claudin 2(CLDN2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (scalegen.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Claudin 2 (CLDN2) in tissue homogenates, cell lysates and other biological fluids. (scalegen.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Claudin 2 (CLDN2) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (scalegen.com)
The impact of snail knockdown on claudin appearance was cell type particular. (hiv-proteases.com)
In earlier studies overexpression of snail and slug decreased the manifestation of claudin 1 mRNA in Madin Darby kidney epithelial cells [7]. (hiv-proteases.com)
Claudin 3 4 and 7 expressions were also shut down by snail in mouse epithelial cell lines [1]. (hiv-proteases.com)
In conclusion however a part of snail's effect on EMT appear to be mediated through downregulation of claudin manifestation although the affects may vary with regards to the specific cell lines. (hiv-proteases.com)

Functional roles of MSI2 and miR-143 on AML cell proliferation and migration were determined by CCK-8 assay, colony formation, and transwell assays in vitro and in mouse subcutaneous xenograft and orthotopic transplantation models in vivo. (biomedcentral.com)

By constructing an EMT model of retinal pigment epithelial (RPE) cells in vitro, we found that autophagy was activated in the EMT process of RPE cells. (ijbs.com)
The in vitro cell viability of hCMEC/D3 cells was measured by cell counting kit-8. (researchsquare.com)

Confluent monolayers of adult human RPE cultures exhibit characteristics of native RPE. (arvojournals.org)

Similar to wild-type (WT) keratinocytes, Tiam1-deficient cells establish primordial E-cadherin-based adhesions, but subsequent junction maturation and membrane sealing are severely impaired. (rupress.org)
Immunologically, AD is driven by TSLP-secretion by keratinocytes and epithelial cells which modulate dendritic cell (DC) and basophil function to preferentially induce a Th2 immune response. (springeropen.com)

This advised that 17-AAG induced cell cycle arrest by preventing Ponatinib selleck chemicals A549 cells from entering mitosis. (hsp90-inhibitors.com)
We assessed the transcription ranges of EGFR, HIF-1A, AKT1 and RAF-1 by real-time RT-PCR following A549 or GLC-82 cells were taken care of with 17-AAGorDMSO for 24 h. (hsp90-inhibitors.com)

Tight junctions (TJ) are multi-protein complexes that hold epithelial cells together and form structural and functional barriers for maintaining proper biological activities. (biomedcentral.com)
In these processes, cancer cells usually have dysfunctional cell-cell junctions, especially in AJ and TJ [ 3 , 4 ]. (biomedcentral.com)
However, cell-cell junctions do not always negatively regulate cancer development. (biomedcentral.com)
Therefore, the regulation of cell-cell junctions is a dynamic process during cancer progression, and the regulatory mechanisms are warranted for further studies. (biomedcentral.com)
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
ZO-1 may be involved in signal transduction at cell-cell junctions. (thermofisher.com)

OCLN had an increased phosphorylation level in DUSP3-deficient cells. (biomedcentral.com)
This Human OCLN ELISA Kit was designed for the quantitative measurement of Human OCLN protein in serum, plasma, tissue homogenates, cell lysates. (cusabio.com)

Although tumors can end up being produced in pets by cells from the peripheral bloodstream of sufferers (14), and murine CTCs possess been effectively cultured (15, 16), LRRFIP1 antibody long lasting lifestyle of singled out individual CTCs provides not 81938-43-4 81938-43-4 really been reported. (biotechnologyconsultinggroup.com)
This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. (cancerindex.org)
These heterozygous somatic mutations most regularly consist of a point mutation within exon 21, leading to an amino acid substitution or in-frame deletions within exon 19.Kinase domain mutations result in constitutive activation of EGFR by jak2 inhibitors selleck chemicals destabilization in the autoinhibited conformation on the receptor.In mutant EGFR tumors, cell survival is dependent on EGFR signaling, a phenomenon termed ?oncogene addiction? (hsp90-inhibitors.com)
We observed that snail was expressed in 22.6% of tumors although its expression varied depending on the histology of the tumors. (hiv-proteases.com)

In a number of cell lines knockdown of snail decreased invasion in both matrigel assay and in the myoma tissues model for invasion. (hiv-proteases.com)
Snail knockdown inspired the appearance of claudins independently within a cell-line reliant manner but didn't impact MMP expressions or activations to any significant level. (hiv-proteases.com)

The brush border on the apical surface of the epithelial cells is covered with glycocalyx, which is composed of oligosaccharides attached to membrane glycoproteins and glycolipids. (wikipedia.org)

Methods: The hCMEC/D3 cells were treated with propofol, followed by TNF-α. (researchsquare.com)

Our study revealed that DUSP3 is an important TJ regulatory protein and its decrease may be involved in progression of epithelial cancers. (biomedcentral.com)
therefore, abnormalities of TJ are frequently observed in epithelial cancers [ 4 ]. (biomedcentral.com)

New Insights into the Pivotal Roles of Claudins in Proximal Tubule Electrolyte Reabsorption Aronson PS . (yale.edu)
New Insights into the Pivotal Roles of Claudins in Proximal Tubule Electrolyte Reabsorption Journal Of The American Society Of Nephrology 2022, 33: 659-661. (yale.edu)

Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kDa) proenzyme. (samatashkhis.com)

ASL glucose concentrations are the net result of diffusion of glucose from blood and interstitial fluid across the respiratory epithelium into the ASL, and removal of glucose from ASL by epithelial glucose transport processes. (ersjournals.com)
The epithelium is composed of a single layer of cells, while the other two layers of the mucosa, the lamina propria and the muscularis mucosae, support and articulate the epithelial layer. (wikipedia.org)
Goblet cells secrete the mucus layer which protects the epithelium from the lumenal contents. (wikipedia.org)

Antibodies recognizing native CLDN6 as displayed on cell surfaces and mediating complement-dependent cytotoxicity were elicited in vaccinated animals. (cusabio.com)

We examined the development of the nervous system in the rhopalium, a medusa-specific sensory CAL-101 cell line structure, in Aurelia sp.1 (Cnidaria, Scyphozoa) using confocal microscopy. (sykinhibitors.com)
At least seven bilaterally arranged neuronal clusters consisting of sensory and ganglion cells and their neuronal processes became evident in the rhopalium during metamorphosis to the medusa stage. (sykinhibitors.com)

We conclude that the observed effects contribute to loss of epithelial integrity during atopic asthma. (springer.com)

CLDN6 enhances the chemoresistance to ADM via activating the AF-6/ERK signaling pathway and up-regulating cancer stem cell characters in MDAMB231 cells. (cusabio.com)
We found that MSI2 was significantly overexpressed in AML and exerted its role of promoting AML cell growth by targeting DLL1 and thereby activating Notch signaling pathway. (biomedcentral.com)
To clarify mechanisms of salivary gland dysfunction, we identified a signaling pathway involved in the dedifferentiation of primary cultures of parotid acinar cells. (sykinhibitors.com)

We further show that the reported increase of Fluozin-3 fluorescence following treatment of cells with 300 µM chloroquine for 1 h may not reflect increased zinc accumulation, since a similar treatment in Madin-Darby canine kidney cells results in a 36% decrease in their total zinc content. (bvsalud.org)

The Toll-like receptors (TLRs), forming one family of pattern-recognition receptors, sense and respond to various stimuli originating from microbial pathogens or injured cells [ 2 ]. (biomedcentral.com)
With this review, we provide an overview on histamine and the expression of its four receptors on skin resident and nonresident cells. (springeropen.com)

Description: tissue homogenates, cell lysates and other biological fluids. (scalegen.com)

Epithelial cells are continuously renewed every 4-5 days through a process of cell division, maturation, and migration. (wikipedia.org)
Cell-cell interaction has been shown to coordinate collective cell migration, which is a critical process in cancer metastasis [ 6 ]. (biomedcentral.com)
We previously described that peripheral administration to neonatal mice of the TLR2 ligand PAM3CSK4 (P3C), a prototypic Gram-positive bacterial lipopeptide, induces the migration of innate immune cells to the CSF. (biomedcentral.com)
They also point to the efficacy of N-acetylcysteine in reducing the deleterious effects of inflammation-associated perinatal injuries by a previously undescribed mechanism, i.e. the inhibition of innate immune cell migration across the choroid plexuses, without interfering with the systemic inflammatory response to infection. (biomedcentral.com)

Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
In primary cultures of human bronchial epithelial cells (HBECs) with 16.6±0.4 mM glucose in the basolateral medium, ASL glucose was 2.2±0.5 mM [ 11 ]. (ersjournals.com)
Primary cell culture of rat IMCD cells on permeable filter supports was performed under different osmotic culture conditions and after stimulation by antidiuretic hormone (AVP). (uni-luebeck.de)
Different tumor cell lines could also present different responses based on specific cell kinetics although the primary reaction between your manifestation of snail and claudins would be that the former would decrease the manifestation of at least some claudins. (hiv-proteases.com)

how a cell can detect, measure and respond to the rigidity of its substrate and how these processes apply to larger biological systems. (mechanobio.info)

The new culture model preserves native RPE morphology, electrophysiology, and gene and protein expression patterns, and may be a useful model to study RPE physiology, disease, and transplantation. (arvojournals.org)
The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)
BOB.1 from "B cell binding Octamer protein 1 "is a specific coactivator of transcription factors Oct-1 and Oct-2 of B lymphocytes. (samatashkhis.com)
Neural factors are a class of protein molecules with neurotrophic activity that can promote the survival and regeneration of nerve cells. (acrobiosystems.com)

Results show that DNA methylation down-regulates CLDN6 expression through MeCP2 binding to the CLDN6 promoter, deacetylating H3 and H4, and altering chromatin structure, consequently promoting migratory and invasive phenotype in breast cancer cells. (cusabio.com)

Paneth cells produce antimicrobial peptides such as human alpha-defensin. (wikipedia.org)
We tested what native features have been preserved with a new culture protocol for adult human RPE. (arvojournals.org)
We cultured RPE from adult human eyes. (arvojournals.org)
It was also demonstrated that propofol inhibited sevoflurane-induced inflammation through NF-κB pathway in human neuroglioma cells [ 15 ]. (researchsquare.com)
We transplanted human peripheral T cells and analyzed priming of CTLs in NOG mice. (sykinhibitors.com)

The gold nanoparticles and DSSCs were investigated by field emission scanning electron microscopy (FE-SEM), ultraviolet-visible MDV3100 price (UV-vis) absorption spectra, current-voltage characteristics, electrochemical impedance spectroscopy (EIS), and incident photon conversion efficiency (IPCE) analyses to study the SPR effect of the gold nanoparticles on the photoelectrodes of the dye-sensitized solar cells. (aktsignals.com)

Conclusions In summary we have studied the expression of snail and and correlated the findings with expression of claudins 1 2 3 4 5 and 7 and matrix metalloproteinases 2 and 9. (hiv-proteases.com)

Importantly, RPE epithelial properties can be maintained by promoting autophagy and effectively reversing TFG-β2-induced RPE fibrosis. (ijbs.com)

Compared to those of the DUSP3-expressing cells, we found the expression and distribution of ZO-1, a TJ-anchoring molecule, were abnormal in DUSP3-deficient cells. (biomedcentral.com)
Accumulating data have confirmed that high expression of Msi2 mRNA is associated with poor prognosis in AML, as patients with as low as 1% of total bone marrow cells expressing elevated MSI2 levels still have a short survival [ 14 , 15 ]. (biomedcentral.com)
In cell line studies snail did not influence the expression or activation of MMP2 or MMP9 to a significant degree. (hiv-proteases.com)
While snail expression was inversely associated with some Ciproxifan claudins the results were somewhat different in the histological data and cell line experiments. (hiv-proteases.com)
We reported previously that the expression pattern of claudins changes during culture, is related to the three-dimensional organization of the cells, and reflects their ability to function as acinar cells. (sykinhibitors.com)

During EMT, the strong AJs formed by E-cadherin are replaced by weak N-cadherin interaction, allowing epithelial cells to escape from the homotypic interactions that tie them to adjacent cells. (biomedcentral.com)

Autophagy is an important cell biological mechanism in maintaining the homeostasis of tissues and cells, resisting the process of EMT. (ijbs.com)
Ab-3 shows no reaction with a variety of normal tissues .CEA is not found in benign glands, stroma, or malignant prostatic cells. (samatashkhis.com)

Microfold cells (commonly referred to as M cells) sample antigens from the lumen and deliver them to the lymphoid tissue associated with the mucosa (MALT). (wikipedia.org)

Resisting cell death', 'genome instability and mutation' and 'deregulating cellular energetics' refine breast cancer classification with their predictive values. (jcancer.org)
The CNS is protected against the invasion of immune cells by cellular barriers which comprise the cerebrovascular walls and the choroid plexuses (ChPs). (biomedcentral.com)
Part of cellular calcium is sequestered in intracellular organelles, including endoplasmic reticulum, sarcoplasmic reticulum (SR) in muscle cells and in mitochondria. (abdominalkey.com)

Circulating growth cells (CTCs) represent the principal trigger of intractable metastatic disease and are regarded important for metastasis development (3-5). (biotechnologyconsultinggroup.com)

J Cell Biol (2005) 170 (7): 1029-1037. (rupress.org)

Viable cells with intact membranes exclude PI, whereas dead and damaged cells with broken membranes are permeable to PI. (hsp90-inhibitors.com)

AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism? (mdpi.com)

Anatomy30

Organisms6

Diseases2

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Phenomena and Processes17

Disciplines and Occupations1

Information Science3

Occludin15

Barrier12

Transition5

Adhesion6

Proteins3

Apoptosis3

Endothelial3

Differentiation5

Polarity3

Intestinal7

Lung6

Transmembrane1

Permeability3

Airway1

Claudin17

Proliferation1

Vitro2

Monolayers1

Keratinocytes2

A5492

Junctions6

OCLN2

Tumors4

Knockdown2

Apical1

Methods1

Cancers2

Pivotal2

Ubiquitously expressed1

Epithelium3

Antibodies1

Sensory2

Integrity1

Signaling pathway3

Kidney1

Receptors2

Tissue homogenates1

Migration4

Primary4

Processes1

Protein5

Phenotype1

Human5

Characteristics1

Findings1

Effectively1

Expression5

Adjacent cells1

Tissues2

Mucosa1

Cellular3

Metastasis1

Biol1

Permeable1

Mechanism1