• Pathway analysis in blood cells of pigs infected with classical swine fever virus: comparison of. (deepdyve.com)
  • Classical swine fever virus replicon particles lacking the E rns gene: a potential marker vaccine for intradermal application. (microbiologyresearch.org)
  • Nevertheless, animals inoculated with an E2 subunit vaccine are unable to prevent horizontal and vertical spread of the virus . (03964.com)
  • Molecular epidemiology studies in these regions suggests that the virus circulating in the field has evolved under the positive selection pressure exerted by the immune response to the vaccine, leading to new attenuated viral variants. (biomedcentral.com)
  • The purpose of this study is to determine whether immune globulin can limit the amount of yellow fever vaccine virus present in the blood after vaccination without compromising the immunit. (bioportfolio.com)
  • Jishu Shi, professor of vaccine immunology and director of U.S.-China Center for Animal Health in the university's College of Veterinary Medicine, has developed a method of producing a classical swine fever vaccine safely and inexpensively. (k-state.edu)
  • Shi's vaccine uses a protein from the virus rather than live or attenuated virus, which means the vaccine poses no biosecurity risk to produce in the U.S., where classical swine fever was eradicated in 1978. (k-state.edu)
  • In contrast to the classical (non-marker) C-strain vaccine, this marker vaccine has the advantage that the accompanying serological test can discriminate between infected and vaccinated animals. (royalsocietypublishing.org)
  • Improved vaccination strategies are urgently needed to curb the global spread of this virus, and the development of suitable vaccine candidates will be aided by an improved understanding of IBV molecular biology. (asm.org)
  • The Laboratory of Vaccine Immunology develops vaccine adjuvants and novel diagnostic assays and vaccines for swine and bovine infectious diseases. (k-state.edu)
  • 2018. Towards the development of a one-dose classical swine fever subunit vaccine: antigen titration, onset and duration of immunity. (k-state.edu)
  • Recombinant viruses represent a particularly promising avenue of vaccine research both for improving existing vaccines and for developing new ones [ 8 , 9 ]. (hindawi.com)
  • How close are we to developing a vaccine for African swine fever? (thepigsite.com)
  • We speak to the head of the African swine fever group at The Pirbright Institute about the hard work going into developing a vaccine that could protect the global pig herd from a deadly foe. (thepigsite.com)
  • Early in June 2019, reports emerged from China that shopping mall operator Guangdong Highsun Group Co had announced it was backing the development of an African swine fever vaccine. (thepigsite.com)
  • Many experts were also sceptical of the research behind the development of the vaccine, and rightly so, as China's Ministry of Agriculture later released a statement that the company had not received a research application and that without experimental data, the claim that a polysaccharide injection is effective in preventing the virus lacked scientific proof. (thepigsite.com)
  • The Pirbright Institute have their very own African swine fever group who are certainly making progress in their ASF vaccine research. (thepigsite.com)
  • We have been conducting research to underpin vaccine development by improving understanding of how the virus evades hosts defences, especially the main antiviral response type I Interferon," explains Dr Dixon. (thepigsite.com)
  • Our second approach for vaccine development involves identifying which of the viruses 170 genes code for proteins that are immunogenic and can induce a cellular or antibody response. (thepigsite.com)
  • Comparative studies on the pathogenicity and tissue distribution of three virulence variants of classical swine fever virus, two field isolates and one vaccine strain, with special regard to immunohistochemical investigations. (nih.gov)
  • Recent research has indicated that the human MxA protein inhibits the life cycles of certain RNA viruses, such as members of the Bunyaviridae family, the Flaviviridae family and others. (beds.ac.uk)
  • The viruses that comprise the Flaviviridae family are small enveloped, single-stranded, positive-sense RNA viruses. (asmscience.org)
  • Examination of interferon (IFN) signaling in infected cells has revealed strategies by which Flaviviridae members attenuate IFN-a/β receptor signaling actions, thus limiting the downstream expression of ISGs that otherwise control virus replication and spread. (asmscience.org)
  • The classical swine fever virus UTR described appears to be longer at the 5' end than other pestivirus UTRs. (wikipedia.org)
  • All the identified virus isolates were classified as ASFV genotype I based on the partial B646L gene (C-terminal end of VP72 gene) and the full E183L gene encoding p54 protein analysis. (bioportfolio.com)
  • If the amino acid motif is similar to that observed for other HPAI isolates, then the isolate being tested should be considered HPAI and the virus is controlled for export. (ufl.edu)
  • Nyalugwe EP, Jones RAC, Barbetti MJ et al (2015) Biological and molecular variation amongst Australian turnip mosaic virus isolates. (springermedizin.de)
  • Hu X, He C, Xiao Y, Xiong X, Nie X (2009) Molecular characterization and detection of recombinant isolates of Potato virus Y from China. (springermedizin.de)
  • Most animals experience acute infection, during which they either die or survive by producing neutralising antibodies to the virus. (nih.gov)
  • This effect could be neutralized by rescue of the inactivated E rns gene and by neutralizing polyclonal antibodies directed against E rns , indicating that this effect was an inherent property of the RNase-negative virus. (asm.org)
  • Antibodies-online offers ELISA kits and antibodies for research of over 150 viral targets and virus like particles to assist researchers investigating virus structure, replication, pathogenesis and evolution . (antibodies-online.com)
  • The immune response to PRRSV infection is characterized by a delayed appearance of neutralizing antibodies ( 31 ), a short cell-mediated immune response ( 34 , 59 ), and slow development of a virus-specific gamma interferon (IFN-γ) response ( 35 ). (asm.org)
  • Virus spread in various organs was followed by virus isolation, by immunohistochemistry, applying monoclonal antibodies in a two-step method and by in situ hybridisation using a digoxigenin-labelled riboprobe. (nih.gov)
  • Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of family Arteriviridae which, along with the Coronaviridae , are classified in the order Nidovirales ( 9 ). (asm.org)
  • 2019. Development of a multiplex real-time RT-PCR assay for simultaneous detection and differentiation of influenza A, B, C, and D viruses. (k-state.edu)
  • Monocistronic mRNAs containing defective hepatitis C virus-like picornavirus internal ribosome entry site elements in their 5′ untranslated regions are efficiently translated in cells by a cap-dependent mechanism. (microbiologyresearch.org)
  • Identification of determinants involved in initiation of hepatitis C virus RNA synthesis by using intergenotypic replicase chimeras. (microbiologyresearch.org)
  • Hepatitis C virus (HCV) is now the most common cause of liver failure and liver transplantation in the United States and Europe. (asmscience.org)
  • Hepatitis C virus (HCV) is the most important cause of transfusion-associated and community-acquired non-A, non-B hepatitis ( 1 , 2 ). (pnas.org)
  • There are 4 major structurally-distinct IRES groups: type 1 (e.g., poliovirus) and type 2 (e.g., encephalomyocarditis virus), which are dissimilar except for a Yn-Xm-AUG motif at their 3′ borders, type 3 (e.g., hepatitis C virus), and type 4 (dicistroviruses). (pnas.org)
  • Viral IRESs are classified into 4 major groups, based on their sequence and structure: type 1 [e.g., poliovirus (PV)], type 2 [e.g., encephalomyocarditis virus (EMCV)], type 3 (e.g., hepatitis C virus), and type 4 (e.g., cricket paralysis virus). (pnas.org)
  • Pestiviruses are small, enveloped, positive-stranded RNA viruses ( 23 ). (asm.org)
  • For six weeks after postnatal infection, most of the piglets remained clinically healthy, despite persistent high virus titres in the serum, tissues, nasal and rectal swabs. (biomedcentral.com)
  • Here, we compare the efficiency of these products for classical swine fever virus RNA extraction from cell culture supernatant, serum, and tonsil. (academicjournals.org)
  • PRV has many nonessential genes which can be replaced with genes encoding heterologous antigens but without deleterious effects on virus propagation. (hindawi.com)
  • however, this subset of genes can be replaced by heterologous genes without affecting infectivity or virus propagation provided the essential genes remain intact. (hindawi.com)
  • Comparison of viraemia- and clinical-based estimates of within- and between-pen transmission of classical swine fever virus from three transmission experiments. (semanticscholar.org)
  • no clinical signs or virus transmission to sentinel animals were observed after 35 days. (bvsalud.org)
  • However, the virus was not associated with any evident Italy-1/10-1 displayed the closest relatedness to atypical clinical signs. (cdc.gov)
  • Unfortunately, due to the fact that PEDV and a different Alphacoronavirus, transmissible gastroenteritis virus (TGEV), have the same epidemiological and clinical features. (springer.com)
  • The range of clinical signs and its clinical similarity to other diseases can make classical swine fever challenging to diagnose. (usda.gov)
  • Cryo EM structure of the rabies virus ribonucleoprotein complex. (nih.gov)
  • In addition to the IFN- responses, induction of both CD4+ T helper cell and CD8+ cytotoxic T cells (CTL) were discernible C activation of the latter was confirmed in a virus-specific cytolytic assay. (03964.com)
  • Here we describe an extremely sensitive assay to analyze the effects of mutations on the virus RNA synthesis machinery in cells of both. (asm.org)
  • The database (DB) of the European Union (EU) and World Organisation for Animal Health (OIE) Reference Laboratory for classical swine fever offers one of the world's largest semi-public virus-specific sequence collections combined with a module for phylogenetic analysis. (mdpi.com)
  • New research from Kansas State University's College of Veterinary Medicine shows how feed plays a significant role in the spread of swine fever virus. (hutchnews.com)
  • It's been demonstrated that contaminated feed and feeding can support the viability of the virus over extended periods," said Scott Dee, of Pipeline Veterinary Services in Minnesota. (hutchnews.com)