Citrobacter rodentium mouse. Medical search. Frequent questions

Mice were held on iron-deficient, normal iron, or high iron diets and after 2 weeks they were orally challenged with the pathogen Citrobacter rodentium. (nih.gov)
For the study, the researchers examined Citrobacter rodentium, an intestinal bacterial pathogen of mice that's used to model infections with human E. coli. (medicalxpress.com)
Citrobacter rodentium is mouse specific extracellular pathogen, which shares an infection strategy with human pathogenic E. coli strains (for example E. coli O157). (ukri.org)
Being a natural mouse pathogen, C. rodentium provides an ideal model to study infections with gut pathogens in the natural host and in the context of the gut microbiota. (ukri.org)
Dietary fiber deprivation, together with a fiber-deprived, mucus-eroding microbiota, promotes greater epithelial access and lethal colitis by the mucosal pathogen, Citrobacter rodentium. (wellnessresources.com)
2015) studied the ability of six different phytogenic compounds on mice' resistance to enteric pathogen infection. (benisonmedia.com)
Figure 1,2&3 shows that mice receiving eugenol (the main component of clove essential oil) had reduced colonization of Citrobacter rodentium, an enteric pathogen. (benisonmedia.com)
Interestingly, the team found that a year later animals that were infected with Citrobacter and had received a single two-week course of dietary iron were alive and healthy, and surprisingly still colonized by the pathogen in their gastrointestinal tract. (sciencemission.com)
Survival and early colonization models show that mice supplemented B12 were more susceptible to pathogen colonization and virulence. (ualberta.ca)

In this study, polymeric Ig receptor (pIgR) knockout mice, which lack secretory antibodies (SAb), were used to investigate the role of vaccine-elicited SAb in protection against gastrointestinal bacterial infections. (ox.ac.uk)
Finally, we investigated the importance of SIgA in protection against recurrent infections with Citrobacter rodentium. (ox.ac.uk)
MNV infects hematopoietic cells and alters their cellular morphology and causes subclinical chronic infections in adult immunocompetent mice. (kitpcr.com)
Working in mice, researchers assessed how immunoglobulin G (IgG) - a type of antibody that resides primarily in the blood - is transferred from a mother's blood to her breast milk, and how IgG protects young mice from Citrobacter rodentium, the rodent equivalent of a pathogenic strain of Escherichia coli that causes severe intestinal infections. (microbiomepost.com)
Then, they infected the pups with Citrobacter rodentium , the rodent equivalent of a pathogenic strain of Escherichia coli that causes severe intestinal infections. (microbiomepost.com)
Finally, the team set out to confer protection against gut infections by inducing IgG antibodies and then immunize female mice with them before the animals became pregnant. (microbiomepost.com)

PA significantly reversed multiple lipids and '-glutamyl amino acids from elevated levels in colitis mice to the baseline. (usda.gov)
Anti-inflammatory and microbiota-modulating effects of PA were further validated in an infectious colitis model induced by Citrobacter rodentium, in which PA also restored the microbial dysbiosis index to the baseline and promoted microbial interactions. (usda.gov)
Consistently, Rag2-/- mice with ILC3-specific deletion of Rptor or Rictor were more resistant to IFN-g-dependent a-CD40 colitis, but exhibited no obvious defects in IL-22-mediated defense against Citrobacter rodentium infection. (unibas.ch)

Liang, Qiaochu et al, Sialic acid plays a pivotal role in licensing Citrobacter rodentium's transition from the intestinal lumen to a mucosal adherent niche, Proceedings of the National Academy of Sciences (2023). (medicalxpress.com)
Vaccination of C57BL/6 mice with Salmonella typhimurium elicited strong antigen-specific, mucosal responses, which blocked in vitro invasion of epithelia. (ox.ac.uk)
C. rodentium are being used as models for studying mucosal response to infection, colon tumor production, and virulence associated with pathogenic E. coli. (up.ac.za)

Citrobacter rodentium is a Gram-negative species of bacteria. (wikipedia.org)
C. rodentium causes a self-limiting infection and triggers robust immune responses, proliferation of epithelial cells and displacement of the normal gut bacteria which mimic the characteristics observed during human infection with pathogenic E. coli. (ukri.org)
Although higher numbers of bacteria were detected early after challenge infection in feces of vaccinated pIgR(-/-) mice compared with vaccinated C57BL/6 mice, both mouse strains showed complete clearance after 9 days. (ox.ac.uk)
Using a gnotobiotic mouse model, in which animals were colonized with a synthetic human gut microbiota composed of fully sequenced commensal bacteria, we elucidated the functional interactions between dietary fiber, the gut microbiota, and the colonic mucus barrier, which serves as a primary defense against enteric pathogens. (wellnessresources.com)
Guess which mice fared better against that pathogenic bacteria? (easyhealthoptions.com)
The team found that gut bacteria are able to induce IgG antibodies in the gut of mice, and the antibodies can be transferred from a mother's blood to her breast milk. (microbiomepost.com)
Researchers report that giving mice dietary iron supplements enabled them to survive a normally lethal bacterial infection and resulted in later generations of those bacteria being less virulent. (sciencemission.com)
Tissue analysis over the course of the experiment showed that both groups of infected mice had comparable levels of bacteria, yet the iron group appeared healthy while the no-iron group got sicker. (sciencemission.com)
To determine if this was the case, the team sequenced the genomes of Citrobacter that were isolated from these animals and found that in the genes necessary for causing disease, the bacteria had accumulated mutations, rendering those genes non-functional. (sciencemission.com)
Studies with germ-free mice (mice raised in sterile conditions with no gut bacteria)are being used to examine the effect of changing microbiota on behavior. (ombrelab.com)

These results suggested that, in immune animals, SIgA is crucial for the protection of gastrointestinal surfaces against secreted bacterial toxins, may inhibit early colonization by C. rodentium, but is not essential for protection against re-infection with S. typhimurium or C. rodentium. (ox.ac.uk)
Since our study shows that increased mucin corresponded with higher levels of C. rodentium colonization, we set out to determine the contributions of mucin to gut ecology and the dietary phytochemicals that stimulate their effects in the gut. (ualberta.ca)

An essential role for specific SAb in protection against Vibrio cholerae was evident from experiments showing that vaccinated pIgR(-/-) mice, but not vaccinated C57BL/6 mice, were susceptible to cholera toxin challenge. (ox.ac.uk)
As adults, these mice were more susceptible to gut inflammation associated with inflammatory bowel disorder. (microbiomepost.com)

In this project we will use wild type and mutant C. rodentium strains as well as wild type mice and mice deficient in a key controllers of cholesterol metabolism. (ukri.org)
To investigate the impact of mTOR signaling on ILC3s, I generated wildtype and Rag2-/- lymphopenic mouse strains with a conditional RORc promoter-driven deletion of Rptor and/or Rictor, the essential subunits of mTORC1 and mTORC2, respectively. (unibas.ch)

To study bacterial pathogenesis and the role of the microbiota during infection, scientists commonly infect mice with Citrobacter rodentium as a robust model system. (ubc.ca)
Acid hydrolysis processing to both cultivars reduced the effect on the microbiota and ability of C. rodentium to colonize the gut. (ualberta.ca)
Germ-free mice fed the proanthocyanidin-rich containing fraction had increased mucin accumulation in the feces, indicating that phytochemicals directly impact the mucus layer independently of the microbiota. (ualberta.ca)
To determine how B12 impacts the gastrointestinal microbiota, we supplemented drinking water at 100 times the amount found in diet and challenged mice with C. rodentium . (ualberta.ca)
In adult germ-free mice, mild external stress resulted in an exaggerated release of stress hormone compared to control mice with normal microbiota. (ombrelab.com)

However, the mice that underproduced serotonin became much sicker after bacterial exposure, often dying from their illness. (utsouthwestern.edu)
Here, we will use the Citrobacter rodentium model to investigate the temporal consequences of bacterial infection on metabolism and immune responses in intestinal epithelial cells (IECs), as well as dysbiosis. (ukri.org)

Previous studies identified one such system in C. rodentium , called Cpx, that when deleted, resulted in the reduction of virulence in mice. (ubc.ca)
These results suggest that while the Cpx regulon contributes significantly to the virulence of C. rodentium , other currently unknown factors likely play a contributing role. (ubc.ca)
Cecal meta-transcriptomics revealed that the activities of the Firmicute population was altered by B12 supplementation and this contributed to a more virulent C. rodentium population as confirmed by reduced glucosidase and increased virulence genes. (ualberta.ca)

Using mice, the researchers studied how serotonin might change the ability for Citrobacter rodentium - a mouse gut bacterium often used as an analog for E. coli in humans - to infect and sicken their hosts. (utsouthwestern.edu)
Researchers wanted to make sure their findings held true in living creatures, so they performed tests on mice as well. (easyhealthoptions.com)
Researchers exposed mice to Citrobacter rodentium, a mouse gut bacterium comparable to E. coli in humans. (easyhealthoptions.com)
Researchers also found that treating mice with a drug that increases serotonin levels known as fluoxetine (Prozac) prevented them from getting sick from this bacterium too. (easyhealthoptions.com)
Researchers also figured out that both E. coli and C. rodentium have a serotonin receptor on their surfaces. (easyhealthoptions.com)
Researchers have now found that a specific type of antibody in breast milk helps establish healthy gut microbiotas in infant mice . (microbiomepost.com)
First, the researchers fed breast milk from mice lacking IgG and breast milk from normal mice to two separate groups of baby mice. (microbiomepost.com)

Modulation of host cytoskeleton function by the enteropathogenic Escherichia coli and Citrobacter rodentium effector protein EspG. (k-state.edu)

Fecal levels of the innate defensive molecules and markers of inflammation lipocalin-2 and calprotectin were not influenced by dietary iron intervention alone, but were markedly lower in mice on the iron-deficient diet after infection. (nih.gov)
Next, mice on the iron-deficient diet tended to gain more weight and to have a lower grade of colon pathology. (nih.gov)
To test the importance of iron metabolism in promoting the cooperative defense system during infection, authors gave a population of mice an LD100 dose of Citrobacter (which should kill 100 percent of the host population) and fed half the population a normal diet and the other half a diet supplemented with iron for only 14 days, after which they were returned to a normal diet. (sciencemission.com)
In this thesis, a mouse model of infectious Citrobacter rodentium was used to challenge the effects of diet, specifically those associated with phytochemicals and vitamin B12 consumption, on intestinal ecology and integrity to promote host health. (ualberta.ca)
In accordance with a previous study, the acid hydrolyzed anthocyanidin fraction reduced weight gain in mice fed a high fat diet. (ualberta.ca)

Genetic and biochemical characterization of Citrobacter rodentium sp. (wikipedia.org)

Recently, by applying state-of-the-art molecular methods to study the interaction of C. rodentium with cells that line the gut we found that the infection causes drastic changes to energy production and central metabolism in the host cells, in what seems to be an attempt to dampen inflammation. (ukri.org)
Additionally, increases in chronic gut inflammation through the administration of a mouse parasite Trichus muris significantly increased anxiety-like behavior and could be reversed with anti-inflammatory drugs or probiotics 10. (ombrelab.com)

These mice were genetically modified to either over- or underproduce serotonin in their gastrointestinal tracts. (utsouthwestern.edu)
Treating mice with fluoxetine (sold under the brand name Prozac) to increase serotonin levels prevented them from getting sick from C. rodentium exposure. (utsouthwestern.edu)
Further experiments identified the receptor for serotonin on the surfaces of both E. coli and C. rodentium , a protein known as CpxA. (utsouthwestern.edu)
The mice used in the study were also genetically modified to either produce extra serotonin or very little serotonin in their gastrointestinal tracts. (easyhealthoptions.com)
On the other hand, mice who were modified to produce very little serotonin got very sick when exposed to C. rodentium - many of them even died. (easyhealthoptions.com)

However, vaccinated C57BL/6 and pIgR(-/-) mice were equally resistant to challenge infection with virulent S. typhimurium. (ox.ac.uk)

She determined that a subset of the genes in the regulon were differentially expressed in the absence of Cpx, and by systematically deleting each of these genes and observing the level of infection in mice, discovered that the deletion of two genes, degP and dsbA, resulted in significantly attenuated infection by C. rodentium . (ubc.ca)
For the current work, the team studied a naturally occurring gastrointestinal infection in mice caused by Citrobacter rodentium ( CR ), which leads to diarrhea, weight loss and, in extreme cases, death. (sciencemission.com)

However, our data also showed decreased intestinal inflammatory responses of mice fed on high iron diets. (nih.gov)
Genome-editing of Mouse Small Intestinal Organoids. (booksdo.com)
Small intestinal ILC3s from Rag2-/- mice exhibited a higher dependency on both mTORCs than small intestinal ILC3s from wildtype mice, indicating that mTOR is differentially regulated in ILC3s from lymphopenic and wildtype mice. (unibas.ch)
To determine the effect of phytochemicals on microbe-host interactions and intestinal health, the seed coat of two cultivars of peas ( Pisum sativum ) rich and poor in phytochemicals were fed to mice as raw or acid hydrolyzed fractions. (ualberta.ca)
In addition, pea phytochemicals increased mucin accumulation in the intestinal lumen, and this may have improved the ability of C. rodentium to colonize the gut. (ualberta.ca)

The team found that pups that received breast milk from mothers lacking IgG became sick after being infected with C. rodentium, while those that received breast milk with IgG antibodies did not. (microbiomepost.com)
So, Zeng and her colleagues sought to explore how IgG antibodies influence the gut microbiotas of newborn mice. (microbiomepost.com)
In pups that received breast milk with IgG, the antibodies stopped C. rodentium from attaching to the lining of the infants' guts, thus helping to prevent infection. (microbiomepost.com)

General Information: Citrobacter rodentium is the causative agent of transmissible murine colonic hyperplasia in mice. (up.ac.za)

Murine norovirus (MNV) is a common viral infection of mice in many research facilities. (kitpcr.com)

Those that overproduced this neurotransmitter were less likely to become colonized by C. rodentium after being exposed to this bacterium or had relatively minor courses of illness. (utsouthwestern.edu)

Isolation from the Mouse Small and Large Intestine. (booksdo.com)

Future studies will build on these findings and further delineate the role of each gene in the Cpx regulon and their collective impacts on the pathogenesis of C. rodentium . (ubc.ca)

N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer. (thermofisher.com)

Recently, additional innate lymphocytes have been discovered named innate lymphoid cells (ILCs) in both humans and mice ( 1-8 ). (pasteur.fr)

We study ILCs development from hematopoietic precursors using mouse models. (pasteur.fr)

They found that the short course of dietary iron caused an acute state of insulin resistance in the mice. (sciencemission.com)

Mice that did not receive IgG from their mothers also developed abnormal gut microbiotas, which led to increased levels of immune cells that produce a molecule linked to inflammatory diseases. (microbiomepost.com)
Although IL-12 was predominantly investigated before the discovery of IL-23, many recent studies using IL-23p19 -/- mice have identified that IL-23 but not IL-12 has a key role in orchestrating an inflammatory cytokine cascade involving enhanced expressed levels of IL-17, TNFα, IFNγ and IL-6 in the intestine [ 11 - 14 ]. (oncotarget.com)

Development of genetic mouse models of Parkinson disease, humanized Park7 mouse models. (public.lu)

Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes. (thermofisher.com)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14968-1-AP (Chromogranin B antibody) at dilution of 1:400 (under 10x lens). (ptglab.com)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14968-1-AP (Chromogranin B antibody) at dilution of 1:400 (under 40x lens). (ptglab.com)

Those mice were less likely to become colonized by C. rodentium or if they were colonized by it, they only got mildly sick. (easyhealthoptions.com)
Using what's known as a systems biology approach, they analyzed the gene activity that was induced in the infected healthy population compared to the infected sick population, as well as the uninfected healthy mice. (sciencemission.com)

These mouse based studies are fundamental in nature, but may provide new insights into the ILC biology that could be applicable to human disease states. (pasteur.fr)
Our proof-of-concept studies have already shown deregulation in the production and release of cholesterol during C. rodentium infection. (ukri.org)

Anatomy2

Organisms9

Diseases5

Chemicals and Drugs4

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes4

Pathogen9

Infections6

Colitis3

Mucosal3

Bacteria10

Colonization2

Susceptible2

Strains2

Microbiota5

Bacterial2

Virulence3

Researchers7

Escherichia1

Diet5

Characterization1

Inflammation2

Serotonin5

Virulent1

Infection in mice2

Intestinal5

Antibodies3

Colonic1

Murine1

Bacterium1

Isolation1

Findings1

Dendritic cells1

Humans1

Study1

Dietary iron1

Inflammatory2

Models1

Analysis3

Sick2

Studies2