Citation needed assay. Medical search. Frequent questions

One approach is to perform an enzyme-linked immunosorbent assay (ELISA) using the same Aβ antibody as both the capture and detection reagent. (alzforum.org)
We standardized an enzyme-linked immunosorbent assay kit with some modifications todiscriminate between primary and secondary dengue infections. (who.int)
Binding of monoclonal antibodies (mAbs) to dNCO bound proteins in enzyme-linked immunosorbent assay (ELISA) was used to evaluate antigenicity of dNCO-protein conjugates. (pdx.edu)
TNF-\alpha (TNF-\alpha-EASIA Kit, DIAsource) and IL-6 (IL-6-EASIA Kit, DIAsource) were studied using the enzyme-linked immunosorbent assay (ELISA) method. (tubitak.gov.tr)
The results of serum testing for anti-Zika virus immunoglobulin M (IgM) antibody performed by the New York State Department of Health Wadsworth Center laboratory was negative using the CDC Zika IgM antibody capture enzyme-linked immunosorbent assay (Zika MAC-ELISA) ( 2 ). (cdc.gov)

In the line 280-282, the author wrote that the primers could be adapted for use in PCR-ELISA to improve sensitivity and capacity of the assay. (peerj.com)
The sensitivity of these tests evaluated in settings with a high prevalence of CHIKV infection is poor (range 1.9%-50.8%) compared with that for reference assays, especially in the acute phase of disease ( 1 - 5 ). (cdc.gov)
The assay's sensitivity exceeds that of previously reported CSF oligomer assays, the scientists said. (alzforum.org)
The goal of the assay optimization was to minimize the amount of antibody required for the assay, to minimize the amount of biological material needed from which the complex is isolated and to achieve the optimum sensitivity towards the peptides of interest. (aacrjournals.org)
Nanoflow liquid chromatography-mass spectrometry (nLC-MS) is a powerful alternative for peptide detection because it provides high sensitivity and specificity without the need for an immunocapture step. (elifesciences.org)
Typically if you are within ± 20 nanometers the assay will still work, but have slightly reduced sensitivity. (bioassaysys.com)
Western blot analysis can be a specific technique but may lack the sensitivity needed to identify animals during preclinical stages of disease. (usda.gov)
The enrichment step increased the sensitivity of the assay 100 fold and produced no false positive results. (usda.gov)
The reactivity, specificity, and sensitivity of LAMP assays were determined and compared to those of conventional PCR. (wayne.edu)
Their sensitivity was 100 copies per reaction versus 10 3 to 10 6 copies per reaction for PCR assays. (wayne.edu)
In summary, the experiments revealed substantial differences of cancer cell lines in PARP1 activity parameters, emphasizing the need to evaluate the sensitivity of each type of cancer in combination with the respective chemotherapeutic agent to PARP inhibition. (uzh.ch)

The Human Activated Carboxylated Osteocalcin ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of both human osteocalcin (1-49) and osteocalcin (1-43) (also referred as N-terminal & mid-regional osteocalcin) levels in serum or plasma samples. (eaglebio.com)
The Eagle Biosciences Human Osteocalcin ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. (eaglebio.com)
Eagle Biosciences has quickly become a leading provider of ELISA assay kits, HPLC assay kits, Molecular Biology assays, antibodies, and proteins. (eaglebio.com)
From ELISA development and plate coating to conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay. (immunochemistry.com)
Zika virus IgM antibodies were not detectable by the CDC Zika MAC-ELISA assay performed at the New York State Department of Health Wadsworth Center. (cdc.gov)

Test performance evaluation of SARS-CoV-2 serological assays. (acoem.org)
Results: Four thousand four hundred and fifty-one serological assays from 1445 individual were assessed. (monash.edu)

Alexa Fluor ® 647 Anti-Mouse IL-2 antibody for use in flow cytometry and immunohistochemistry assays. (southernbiotech.com)
We explored the function of cucurmosin in BxPC-3 pancreatic cancer cells using multiple cellular and molecular approaches such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, reverse transcription polymerase chain reaction (RT-PCR), Western blotting and transmission electron microscopy for observing typical changes and formation of apoptotic bodies. (spandidos-publications.com)

If the seropositive samples for HSV-1 and HSV-2 were still stored, we strongly advice that HerpeSelect® 1/2 and Euroline-WB assays should be performed and further analyzed the relationship between antibody titers to herpes simplex virus and different storage conditions. (who.int)
Diagnosis of disease is made by detection of the abnormal form of the prion protein (PrP-Sc) by various antibody-based assays. (usda.gov)
The diagnosis of MG is suggested by clinical history and is confirmed by electromyography, anticholinesterase test or serological assay for acetylcholine receptor antibody. (ispub.com)

The abstract needs more detail and structure conforms to PeerJ standards. (peerj.com)

Rapid and appropriate diagnostic tools are needed to slow or stop the worldwide spread of CHIKV. (cdc.gov)
An introduction to procedures for the identification of antigenic determinants is followed by a review of progress in the development of diagnostic assays at the B cell and T cell levels. (who.int)
Applications in the diagnosis of HIV infection are assessed against the drawbacks of current diagnostic tests, particularly concerning the need for expensive confirmatory tests and the serious problems caused by false-negative responses. (who.int)
Chemists are playing an important role in designing and fabricating new materials for application in diagnostic assays. (typeset.io)
I demonstrate this goal by developing different methods and devices to (1) separate membrane bound proteins with the use of microfluidics, (2) use optical technology to make fiber optic cables into protein sensors, (3) generate new fluidic devices using semiconductor material to manipulate single cells, and (4) develop a new genetic microfluidic based diagnostic assay that works with current PCR methodology to provide faster and cheaper results. (caltech.edu)
The goals for research on surveillance and clinical diagnostics include rapid diagnostic assays for common pathogens and biological warfare agents. (nationalacademies.org)

If the tissue is unsuitable for this assay or if confirmatory testing is needed, the western blot assay is used. (usda.gov)
In the paper, the authors describe the use of a modified western blot assay for elk and sheep tissues. (usda.gov)
In this paper, the authors evaluate an enrichment technique applied to tissue lysates before western blot assay. (usda.gov)

Readers learn how the ability to produce synthetic antigens has opened exciting prospects for the design of new screening reagents and the development of urgently needed assays for monitoring the progress of infection and therapy, for routinely following up asymptomatic carriers, and for establishing a prognosis for carriers and patients at various stages of disease severity. (who.int)
Potent therapeutic strategies are urgently needed for pancreatic cancer. (spandidos-publications.com)

Rapid point-of-need assays are used to detect abundant biomarkers. (psu.edu)
Sensitive and accurate tests are needed to detect Shiga toxin-producing Escherichia coli (STEC) contaminated meat because STEC are dangerous pathogens. (usda.gov)
Data from this study shows the ability of the standardized assays to specifically detect the strains of each target serogroup and, most importantly, to differentiate the strains present into potentially virulent or avirulent groups. (usda.gov)
In the current study, we developed a rapid, simple, and cost-effective assay to detect serotypes in the 23-valent pneumococcal polysaccharide vaccine (PPSV23) using the LAMP method. (wayne.edu)
Real time PCR assays to detect common mutations in the biotinidase gene and application of mutational analysis to newborn screening for biotinidase deficiency. (medlineplus.gov)

The feasibility of LAMP assays in clinical application in patients with invasive pneumococcal diseases was validated by defining the detection limit of the LAMP assay with bacterial genomic DNA-spiked blood specimens. (wayne.edu)
As the technology has moved from research laboratories to clinical laboratories, the need for standardization has increased. (cdc.gov)

Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.BioAssay System's non-radioactive, fluorimetric ACP assay is based on the cleavage of methylumbelliferyl phosphate. (bioassaysys.com)

Accurate and rapid detection of CHIKV infection by reliable point-of-care (POC) assays has been recommended to facilitate outbreak control. (cdc.gov)
Presenter's Email: [email protected] XXX (no-need to enter paper number) The aim of this study is to identify Entamoeba species infecting wild and semi-wild orangutans and any risk factors associated with infection. (muni.cz)
The prevalence of HBV chronic infection among pregnant women in Bor, Jonglei State, is high hence there is a need for established public health interventions that can lead to a reduction of HBV vertical transmission. (bvsalud.org)

The key of the study was the stability of antibodies to herpes simplex virus types 1 and 2, which were measured by HerpeSelect® 1/2 and Euroline-WB assays (2). (who.int)
Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. (southernbiotech.com)
Two approaches are most commonly used to test for anti-SARS-CoV-2 antibodies: lateral flow assays (LFAs) and ELISAs. (acoem.org)
There needs to be proof that tests are not demonstrating antibodies to the four coronaviruses that cause "common cold" symptoms - 229E, OC43, NL63 and HKU1 - which are highly prevalent. (acoem.org)
Further study is needed to show whether antibodies produced against SARS-CoV-2 are neutralizing antibodies that could produce immunity . (acoem.org)
The assay utilizes the two-site "sandwich" technique with two selected antibodies that bind to different epitopes of human osteocalcin. (eaglebio.com)

Cell migration and glucose uptake assays combined with protein function inhibitor studies provided further support that hypoxia-driven adaption of pathways associated with glycolytic metabolism, collagen biosynthesis and remodeling, redox regulation and immunomodulatory proteins typify a proteotype associated with an aggressive cancer cell phenotype. (nature.com)
For these cells to move through the body, they need to break down structural proteins that surround cells. (uwo.ca)

Participants who could not provide consent, did not live independently, or did not have biological marker assay results were excluded. (nih.gov)
Its biological activity has been verified using cellular assays. (invivogen.com)
biological environments and experiments tend to need aqueous environments. (caltech.edu)

Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. (southernbiotech.com)
Cathepsin B and L enzymatic activity was analysed by flow cytometric measurement using Magic Red Cathepsin L and B assay kits (ImmunoChemistry Technologies) according to manufacturer's instruction and modified protocol. (immunochemistry.com)

The aim of this study was develop a sybr-green based quantitative PCR assay for a highly conserved and single copy, putative RNA binding protein, DRBD3. (peerj.com)
Blood samples taken pre-, post-, and 24, 48, 72, and 96 hr postexercise were assayed for comparison of muscle damage and oxidative-stress biomarkers including protein carbonyls (PCs). (umt.edu)
In the last 10 years the field of molecular diagnostics has witnessed an explosion of interest in the use of nanomaterials in assays for gases, metal ions, and DNA and protein markers for many diseases. (typeset.io)
Therefore, the identification of hypoxia-inducible protein targets responsible for cancer progression, malignant transformation and metastasis is needed for deepening our understanding of OS biology. (nature.com)

Does the assay kit work in particular species? (bioassaysys.com)
Yes, this assay works in all species. (bioassaysys.com)
This study aims to identify the species of Entamoeba infecting orangutans by carrying out PCR assays on approximately 400 faecal samples collected by the Orangutan Health Project. (muni.cz)
The phylogeny, relationships, intraspecific and interspecific variation, and even identity of other species and cultivars in other parts of the world still need to be established and validated. (cloudfront.net)

The recent Zika virus disease outbreak in South America also highlights the worldwide need for rapid reliable POC tests. (cdc.gov)
Zika virus RNA was detected in both serum and urine by real-time reverse transcription-polymerase chain reaction (rRT-PCR) performed at the DOHMH Public Health Laboratory using a test based on an assay developed at CDC ( 1 ). (cdc.gov)
Further studies are needed to determine the characteristics of Zika virus shedding in the genital tract and vaginal fluid of humans. (cdc.gov)

Yes, unused reagents can be stored according to the assay protocol. (bioassaysys.com)

citation needed] Warami, Urowayino (7 January 2019). (wikipedia.org)

To meet this need, rapid CHIKV IgM POC tests are now available, but little information exists regarding their performance. (cdc.gov)
However, our findings, in agreement with those of others, show that current rapid CHIKV tests perform poorly and need major improvement ( Table ) ( 1 - 5 ). (cdc.gov)
In conclusion, a rapid and simple LAMP-based PPSV23-targeted serotype detection assay was developed for use in many countries. (wayne.edu)
These designated research organizations should address both classified and unclassified issues, and special mechanisms for rapid funding should be created to support external research efforts as the needs and opportunities emerge. (nationalacademies.org)

In addition, intact cells were challenged with H2O2 alone or in combination with the PARP inhibitor PJ-34, and finally, viability assays were conducted with H2O2 and temozolomide each with PJ-34. (uzh.ch)

Clinicians and researchers currently use several methods to measure estrogen, including Immunoassays and mass spectrometry-based assays. (medicalresearch.com)

The trinitrobenzene sulfonic acid assay (TNBS) and SDS gel electrophoresis were used to evaluate extent of intra and intermolecular cross-linking in dNCO-HSA conjugates. (pdx.edu)
Research is needed to evaluate the different approaches for validation of estrogen assay accuracy. (medicalresearch.com)

The two standardized assays were extensively validated using 215 pure culture strains, laboratory inoculated food samples, and naturally contaminated beef (n = 84) and pork (n = 84) enrichments collected from the red meat surveillance program. (usda.gov)
The assays standardized in this study can be helpful for food surveillance programs and help mitigate product loss due to the presence of avirulent strains lacking crucial virulence genes (stx and eae). (usda.gov)
Further evaluation of this assay is needed through surveillance and vaccine efficacy studies. (wayne.edu)

The development of in situ signal amplification reactions could extend these assays to screening and triaging of patients for trace levels of biomarkers, even in resource-limited settings. (psu.edu)

The researchers turned to a more sensitive assay developed by Singulex of Alameda, California. (alzforum.org)
The assay is not currently available to researchers outside Merck. (alzforum.org)
As they select from among the current assays, clinicians and researchers should consider the purpose of the test and select the most appropriate estrogen measurement method for the situation. (medicalresearch.com)

Diotallevi A ( 2018 ) Peer Review #2 of 'A real-time PCR assay for quantification of parasite burden in murine models of leishmaniasis (v0.2)' . (peerj.com)
Anonymous Reviewer ( 2018 ) Peer Review #1 of 'A real-time PCR assay for quantification of parasite burden in murine models of leishmaniasis (v0.1)' . (peerj.com)
Explore our selection of November citations from 2018. (immunochemistry.com)

The output of these UH2/UH3 Target Validation Projects, to be facilitated by a Resource and Coordinating Center (RCC) funded under a companion RFA-RM-14-017, will be a toolkit of validated and documented assays of specific targets in the domain of stress reactivity and stress resilience, and experimental manipulations/interventions that engage those targets. (nih.gov)

A further 72 samples confirmed the assay was able distinguish the two populations, with less overlap between the groups than the field is used to seeing with CSF monomer ELISAs. (alzforum.org)
Tests need to distinguish between these viruses and SARS-CoV-2. (acoem.org)

This paper characterizes the ability of allylic ethers to provide an amplified response, and offers insight into additional design considerations that are needed before in situ small molecule-based signal amplification becomes a viable strategy for point-of-need diagnostics. (psu.edu)

Current studies on assays of dNCO-specific IgE and IgG lack or have limited characterization of the conjugates used. (pdx.edu)

Our preclinical studies of CAR(2448) suggest its potential for the unmet need of novel strategies for the treatment of ovarian cancer. (lonza.com)
Further studies are needed to confirm the efficacy of PMX-DHP treatment for septic shock. (accjournal.org)
However, to definitely conclude this, we need further extensive studies. (tubitak.gov.tr)
Studies and citations listed under the topics 'gold nanoparticle' and 'toxicity' in the Web of Science database. (medscape.com)

The low prevalence of detectable dNCO specific IgE has been attributed to both assay limitations and a potential IgE-independent dNCO asthma mechanism(s). (pdx.edu)

How do I prepare cell or tissue samples for assays? (bioassaysys.com)

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you. (bioassaysys.com)

Two high-resolution melting (HRM)real-time PCR assays were standardized for the specific detection and discrimination of potentially virulent and avirulent strains of E. coli O103 and O121. (usda.gov)
Both HRM assays showed 100% inclusivity and exclusivity using pure culture strains and enriched spiked food samples. (usda.gov)
The specificity of each LAMP assay was determined using 44 serotypes of pneumococcal strains. (wayne.edu)

Further study is needed to understand the mechanisms responsible for the observed fast-twitch muscle-fiber oxidative-stress relationship. (umt.edu)
This study is the first report of a LAMP-based assay for identification of PPSV23 serotypes. (wayne.edu)
Eight patients underwent thymectomy from January 2003 to October 2005, who were not considered to be difficult intubation, did not have pre-existing pulmonary disease and were not in crisis or in need of preoperative ventilatory support were enrolled for the study. (ispub.com)
The same serologic assay was used in this study and the 2 previous US serosurveys, suggesting that the different results are not an artifact of different assays. (cdc.gov)

This research highlights the need for action on recommendations and interventions to prevent maternal deaths from homicide. (nih.gov)
LCNTDR recognises the huge progress made in combatting NTDs since its inception, but also that a commitment to research and innovation is needed to achieve and sustain 2030 road map targets. (biomedcentral.com)
Intense research has been fueled by the need for practical, robust, and highly sensitive and selective detection agents that can address the deficiencies of conventional technologies. (typeset.io)
Accurate estrogen tests ensure consistent patient assessment and care, reduce the need for retesting of patients, and enable the implementation of research findings in patient care. (medicalresearch.com)
Several overarching strategies are needed to provide the necessary funding for research and development (R&D), mechanisms for response, integration of efforts, and translation of findings into application. (nationalacademies.org)

In addition, it is recommended that we should shift the focus of toxicological experiments from 'live-dead' assays to the assessment of cell function, as this strategy allows observation of bioresponses at lower doses that are more relevant for in vivo scenarios. (medscape.com)

citation needed] Asaba-Assay is situated on a flood basin which is almost always flooded, this results to a large population of mosquitoes and other insects thus making life difficult. (wikipedia.org)
The modified technique showed no false positive results and was shown to be a useful assay for confirming the diagnosis of scrapie and chronic wasting disease in deer and elk. (usda.gov)

In collaboration with groups in Norway and in the U.K., Savage will next test the assay in longitudinal cohorts that also collect other fluid, imaging, and cognitive markers. (alzforum.org)

Thus, there is an urgent need for new therapeutic strategies aiming to control metastasis in OS. (nature.com)

To this end we developed a system that combines in vivo optogenetics with microdialysis and a highly sensitive mass spectrometry-based assay to measure opioid peptide release in freely moving rodents. (elifesciences.org)
Since Assay Buffer is very light sensitive, always keeps buffer in the dark (amber bottle or tube) as much as possible for long term usage. (bioassaysys.com)

Therefore, there is need to develop a test that can distinguishbetween primary and secondary serological responses. (who.int)
Electron donor behaviors of bucillamine were studied by a standard ABTS test method and a chemiluminescence (CL) assay. (nel.edu)

Äggcell eller ovum är en haploid honlig reproducerande cell eller könscell. (drake.nu)
The spacer length of CAR(2448) was optimised based on in vitro cytotoxic activity against ovarian cancer (OC) cell lines via a real-time cytotoxicity assay. (lonza.com)
The proteome needed to support an aggressive osteosarcoma cell phenotype remains largely undefined. (nature.com)
In vitro plasminogen activation, invasion, proteolysis, and tube formation assays were performed in each cell type and TAFI-specific TM was found to reduce metastatic and angiogenic potential. (uwo.ca)
The need for CD4+ T-cell testing services has increased and is expected to continue to increase. (cdc.gov)
See a heatmap of connections between individual perturbagens in cell lines and all other perturbagens used for the P100 assay or the GCP assay. (clue.io)

In the phrase (lines 269-272) "Using a qPCR approach to amplify parasite DNA allows determination of parasite burden in as little as 2-4 hours instead of waiting approximately a week for parasites to grow in the LDA" the authors need consider the extraction time. (peerj.com)

Supplemental testing is useful when tissues are autolyzed or if confirmatory testing is needed. (usda.gov)

Assay standards, controls and samples are added directly to wells of a microtiter plate that is coated with streptavidin. (eaglebio.com)

Homogeneous "mix-incubate-measure" type assay. (bioassaysys.com)

This section needs additional medical references for verification . (wikipedia.org)

[ 13 ] Hence, this article aims to provide general guidelines for nanotoxicological assays of AuNPs. (medscape.com)

The assays are in early stages, and the designers are planning more work to validate them. (alzforum.org)

Of primary interest is the array of enzymes that degrade radiolabeled angiotensins in receptor binding assays. (nova.edu)
The body needs free biotin to activate enzymes called biotin-dependent carboxylases. (medlineplus.gov)

This document has been developed by CDC to give guidance to laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. (cdc.gov)

A post hoc analysis of EUPHRATES (Evaluating the Use of Polymyxin B Hemoperfusion in Randomized Controlled Trial of Adults Treated for Endotoxemia and Septic Shock) trial has shown that PMX-DHP significantly reduced 28-day mortality compared with the control group in septic shock patients with endotoxin activity assay level between 0.60 and 0.89. (accjournal.org)
None of our patients needed ventilatory assistance in the postoperative period. (ispub.com)

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