Loading...
  • tissue
  • The patient's own stored stem cells are then transfused into his/her bloodstream, where they replace destroyed tissue and resume the patient's normal blood cell production. (wikipedia.org)
  • We reasoned that molecules responsible for the stem cell supportive phenotype would be preferentially expressed in AFT024 compared with nonsupporting lines derived from the same tissue source. (pnas.org)
  • The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early hematopoietic and vascular-associated tissue. (wikipedia.org)
  • Immune cells (white blood cells) in the donated tissue (the graft) recognize the recipient (the host) as foreign (nonself). (wikipedia.org)
  • citation needed] Possible mechanisms of recovery include: Generation of heart muscle cells Stimulation of growth of new blood vessels to repopulate damaged heart tissue Secretion of growth factors Assistance via some other mechanism It may be possible to have adult bone-marrow cells differentiate into heart muscle cells. (wikipedia.org)
  • The phenomena of mechanically dissociated cells aggregating and reorganizing to reform the tissue they were obtained from subsequently led to the development of the differential adhesion hypothesis by Malcolm Steinberg. (wikipedia.org)
  • With the advent of the field of stem cell biology, the potential of stem cells to form organs in vitro was realized early on with the observation that when stem cells form teratomas or embryoid body, the differentiated cells can organize into different structures resembling those found in multiple tissue types. (wikipedia.org)
  • Furthermore, there are vast developments in tissue engineering, which involve leveraging of technologies from biomaterials, molecular medicine, biochemistry, nanotechnology, genetic and biomedical engineering for regeneration and cell expansion targets to restructure and/or repair human organs. (wikipedia.org)
  • However, cardiac remodling remains limited due to limited cell residency, impact of mechanical forces onto cell survival and tissue hypoxia. (wikipedia.org)
  • Cardiac tissue engineering is a new technology based on the use of combinations of cells with regenerative capacity, biological and/or synthetic materials, cell signaling agents to induce the regeneration of an organ or damaged tissue. (wikipedia.org)
  • engraftment
  • The cells expanded from enriched cells in the absence of IL-3 and IL-1 revealed engraftment at 2, 4, 5, and 6 months, whereas addition of IL-3 or IL-1 to the cultures significantly reduced the reconstituting ability. (pnas.org)
  • molecules
  • We hypothesize that there are yet to be discovered regulators, and that the stem cell supportive ability of AFT024 comes from many interacting molecules. (pnas.org)
  • HLA-identical siblings or HLA-identical unrelated donors often have genetically different proteins (called minor histocompatibility antigens) that can be presented by Major histocompatibility complex (MHC) molecules to the donor's T-cells, which see these antigens as foreign and so mount an immune response. (wikipedia.org)
  • This initial phosphorylation event further activates the intrinsic tyrosine kinase activity, which in turn phosphorylates and activates signal transduction molecules that propagate the signal in the cell. (wikipedia.org)
  • Cluster of differentiation (CD) molecules are markers on the cell surface, as recognized by specific sets of antibodies, used to identify the cell type, stage of differentiation and activity of a cell. (wikipedia.org)
  • The molecules and exosomes released from stem cells are also being studied in an effort to make medications. (wikipedia.org)
  • lineages
  • Provided are compositions of matter, protocols and methods of use by which inhibiting expression of NR2F6 or activity thereof promotes differentiation of selective hematopoietic lineages, or conversely overexpression of NR2F6 or activity thereof inhibits differentiation. (freepatentsonline.com)
  • In one embodiment inhibition of differentiation is performed in other cell lineages besides hematopoietic. (freepatentsonline.com)
  • The hematopoietic stem cell (HSC) derived from adult bone marrow (BM) is commonly thought to have multilineage potential, meaning that the HSC is considered capable of reconstituting all lymphoid, myeloid, and erythroid lineages of the immune system ( 1 , 2 ). (pnas.org)
  • reconstitute
  • Transferring adult BM into lethally irradiated recipients readily reconstitutes B-2 and MZ, which represent the majority of the B cells in spleen and other lymphoid organs but only poorly reconstitute B-1 cells in the same recipients. (pnas.org)
  • In contrast, B-1a cells are relatively well reconstituted by transfers of HSC populations sorted from "neonatal" BM (2.5 wk of age), although the sorted cells still predominantly reconstitute B-2 and B-1b ( 5 ). (pnas.org)
  • tumors
  • Beyond the hematopoietic system, Usp3{delta}/{delta} animals spontaneously developed tumors, and primary Usp3{delta}/{delta} cells failed to preserve chromosomal integrity. (mdc-berlin.de)
  • mediate
  • To characterize the specific cell types that mediate stem cell expansion, we dissected this microenvironment into a panel of over 200 cloned stromal cell lines ( 7 , 8 ). (pnas.org)
  • It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • The paracrine soluble factors produced by stem cells, known as the stem cell secretome, has been found to be the predominant mechanism by which stem cell-based therapies mediate their effects in degenerative, auto-immune and inflammatory diseases. (wikipedia.org)
  • conversely
  • Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
  • mice
  • Brpf1 -deficient mice display postnatal lethality and severe hematopoietic hypoplasia. (jci.org)
  • Lineage-negative, Ly-6A/E+, c-kit+ bone marrow cells from male mice were cultured in suspension in the presence of stem cell factor, IL-6, IL-11, and erythropoietin with or without IL-3 or IL-1. (pnas.org)
  • The reconstituting ability of the cultured cells was tested by transplanting the expanded male cells into lethally irradiated female mice. (pnas.org)
  • vitro
  • The most potent stromal cell line, AFT024, maintains highly purified fetal and adult stem cell populations in vitro , for at least 4-7 weeks. (pnas.org)
  • Despite their efficient functionality throughout life in vivo , controlling HSC behaviour in vitro (including their proliferation and differentiation) is still a major task that has not been resolved with standard cell culture systems. (rsc.org)
  • Attempts to create organs in vitro started with one of the first dissociation-reaggregation experiment where Henry Van Peters Wilson demonstrated that mechanically dissociated sponge cells can reaggregate and self-organize to generate a whole organism. (wikipedia.org)
  • Stem cells resembling totipotent blastomeres from 2-cell stage embryos can arise spontaneously in mouse embryonic stem cell cultures and also can be induced to arise more frequently in vitro through down-regulation of the chromatin assembly activity of CAF-1. (wikipedia.org)
  • thymic
  • One way to measure thymic function is through T-cell receptor excision circle (TREC) quantification. (frontiersin.org)
  • Nonetheless, decreased thymic output with aging may not affect peripheral T-lymphocytes, because there is a strong homeostatic compensatory mechanism that stimulates independent expansion of mature T-cells from the thymus. (frontiersin.org)
  • severe
  • Candidates for HSCTs include pediatric cases where the patient has an inborn defect such as severe combined immunodeficiency or congenital neutropenia with defective stem cells, and also children or adults with aplastic anemia who have lost their stem cells after birth. (wikipedia.org)
  • citation needed] Acute GvHD of the GI tract can result in severe intestinal inflammation, sloughing of the mucosal membrane, severe diarrhea, abdominal pain, nausea, and vomiting. (wikipedia.org)
  • citation needed] Mucosal damage to the vagina can result in severe pain and scarring, and appears in both acute and chronic GvHD. (wikipedia.org)
  • In 2014, the European Medicines Agency recommended approval of limbal stem cells for people with severe limbal stem cell deficiency due to burns in the eye. (wikipedia.org)
  • Stems cells are being studied in those with severe heart disease. (wikipedia.org)
  • Infection
  • These HSC populations can replenish circulating immune cells during periods of high demand, such as systemic bacterial infection. (sciencemag.org)
  • A. Burberry, M. Y. Zeng, L. Ding, I. Wicks, N. Inohara, S. J. Morrison, G. Núñez, Infection mobilizes hematopoietic stem cells through cooperative NOD-like receptor and Toll-like receptor signaling. (sciencemag.org)
  • Squamous cell cancers of the tonsils are more strongly associated with human papillomavirus infection than are cancers of other regions of the head and neck. (wikipedia.org)
  • regulation
  • Commentary by Fritz addresses the implications of NOD signaling in the regulation of stem cell function. (sciencemag.org)
  • Nevertheless, the biophysical regulation of these cells is still poorly understood. (rsc.org)
  • However, it has been shown that genes responsible for cytokine signaling, inflammatory response, and regulation of cell cycle are differentially expressed in patients with fatal GvHD versus "indolent" GvHD. (wikipedia.org)
  • thymus
  • Newer research indicates that other graft-versus-host-disease target organs include the immune system (the hematopoietic system, e.g., the bone marrow and the thymus) itself, and the lungs in the form of immune-mediated pneumonitis. (wikipedia.org)
  • T-cell receptor excision circles (TRECs) are small circles of DNA created in T-cells during their passage through the thymus as they rearrange their TCR genes. (wikipedia.org)
  • iPSCs
  • This was then followed in 2007 by the successful induction of human iPSCs derived from human dermal fibroblasts using methods similar to those used for the induction of mouse cells. (wikipedia.org)
  • Signaling
  • That NOD signaling influences stem cell function was also reported by Nigro et al . (sciencemag.org)
  • who found that signaling induced by a microbe-derived agonist of NOD2 promoted survival of intestinal stem cells and improved their resistance to oxidative stress-induced cell death. (sciencemag.org)
  • Signaling through CD135 plays a role in cell survival, proliferation, and differentiation. (wikipedia.org)
  • receptor
  • However, this expansion will result in a limited T-cell receptor (TCR) repertoire with a decreased ability to adapt to new antigens and infectious agents ( 3 ). (frontiersin.org)
  • modulation
  • The invention provides means of manipulating hematopoietic stem cell differentiation by modulation of levels of NR2F6 (EAR2). (freepatentsonline.com)
  • at the University of Illinois at Urbana-Champaign demonstrated that cardiovascular organoids can be formed from ES cells through modulation of the substrate stiffness, to which they adhere. (wikipedia.org)
  • regeneration
  • G. Nigro, R. Rossi, P. H. Commere, P. Jay, P. J. Sansonetti, The cytosolic bacterial peptidoglycan sensor Nod2 affords stem cell protection and links microbes to gut epithelial regeneration. (sciencemag.org)