N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. (wikipedia.org)
The long term goal of our research is to elucidate the molecular mechanisms by which proteins are targeted to specific and distinct compartments. (stanford.edu)
Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. (bvsalud.org)
20171
2017). Moreover, Golgi-associated Rab29 can recruit LRRK2 to the surface of the Golgi and activate it there for both auto- and Rab substrate phosphorylation. (stanford.edu)
Humans2
NSFL1 cofactor p47 is a protein that in humans is encoded by the NSFL1C gene. (wikipedia.org)
We also study the NPC1 protein that is essential for cholesterol transport in humans and can lead to Niemann Pick C disease when mutated. (stanford.edu)
Important1
The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. (bvsalud.org)
Cell1
The download Hanging Sam: A Military Biography of General of Insulin like Growth Factor Binding Proteins( IGFBPs) phase 50 response pathway good research with reviewed N cell and C formation enzymes binding for conjugating Insulin like Growth Factors I and II( IGF I and IGF II). (evakoch.com)
Endoplasmic reticulum5
Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein. (wikidata.org)
Some of the ZG enzymes form protein complexes already within the lumen of the endoplasmic reticulum. (medscape.com)
We uncover striking trafficking differences between caveolins, with CAV1 rapidly exported to the Golgi and PM while CAV3 is initially retained in the endoplasmic reticulum and laterally moves into lipid droplets. (bvsalud.org)
The levels of caveolins in the endoplasmic reticulum are controlled by proteasomal degradation, and only monomeric/low oligomeric caveolins are exported into the cis-Golgi with higher-order oligomers assembling beyond this compartment. (bvsalud.org)
The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). (nih.gov)
Stacks1
A cell-free system that mimics the reassembly of Golgi stacks at the end of mitosis requires two ATPases, NSF and p97, to rebuild Golgi cisternae. (nih.gov)
Soluble1
[ 6 , 21 ] Several soluble N-ethylmaleimide-sensitive factor-activating protein receptor (SNARE) proteins, Rab GTPases and aquaporins are suggested to be involved in granule docking/priming, granule swelling and exocytosis. (medscape.com)
Receptor4
[ 11-14 ] Whereas the selective aggregation of ZG proteins has been well documented (Figure 1D & E), their interaction with the TGN/ZG membrane is poorly understood at the molecular level, and neither a common sorting signal nor a sorting receptor has been identified so far. (medscape.com)
In an active model for sorting ('sorting by entry'), membrane binding of secretory proteins is assumed to depend on a 'sorting receptor' within the TGN (e.g., a transmembrane protein), and entry into forming granules is restricted to receptor-mediated trafficking. (medscape.com)
In this model, a receptor is not required and entry into the forming granule is not solely restricted to regulated secretory proteins. (medscape.com)
GPR15 is a G protein-coupled receptor involved in immune disorders such as human immunodeficiency virus-induced enteropathy, multiple sclerosis, and colitis. (bvsalud.org)
Membranes1
Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. (nih.gov)
Cytosolic1
A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. (wikipedia.org)
Mediate1
however, some information is beginning to emerge regarding the proteins that mediate the exocytotic membrane fusion event. (medscape.com)
Trans1
This process takes place at the trans -Golgi network (TGN) and is thought to involve selective protein aggregation and protein-membrane interactions. (medscape.com)
Membrane fusion1
The p47 co-factor regulates the ATPase activity of the membrane fusion protein, p97. (nih.gov)
Binds1
The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. (nih.gov)
Mitosis1
This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. (nih.gov)
Clathrin2
The nonsecretory proteins fail to aggregate and are removed from the maturing granules in a clathrin-dependent process (reviewed in [ 15 , 16 ] ). (medscape.com)
These condensing vacuoles (CVs), the initial stage of ZGs, then pinch off as immature granules, which mature into ZGs by the selective removal of nonsecretory granule proteins (via a clathrin-mediated process called 'constitutive-like secretion') and further condensation of the aggregated proteins occurs, as well as a reduction in granule size. (medscape.com)
Structural2
We show that P132 is positioned at a major site of protomer-protomer interactions within the CAV1 complex, providing a structural explanation for why the mutant protein fails to homo-oligomerize correctly. (bvsalud.org)
the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). (nih.gov)
Complex2
Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion. (nih.gov)
[ 8 , 9 ] These complexes exhibit distinct protein compositions and contain certain zymogens in association with nonenzyme proteins, which have been proposed to act as 'helper' proteins in complex formation, aggregation and/or sorting to the membrane. (medscape.com)
NSFL1C2
NSFL1 cofactor p47 is a protein that in humans is encoded by the NSFL1C gene. (wikipedia.org)
NSFL1C has been shown to interact with Valosin-containing protein. (wikipedia.org)
Fusion1
Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack. (wikidata.org)
Studies1
Ultrastructural studies indicate that parts of the Golgi cisternae become dilated and filled with electron-opaque material. (medscape.com)
Signal2
Probe Set ID Ref Seq Protein ID Signal Strength Name Gene Symbol Species Function Swiss-Prot ID Amino Acid Sequence 1367452_at NP_598278 7.9 small ubiquitin-related modifier 2 precursor Sumo2 Rattus norvegicus " Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. (nih.gov)
Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins (By similarity). (nih.gov)
Formation1
Caveolin-1 (CAV1) and CAV3 are membrane-sculpting proteins driving the formation of the plasma membrane (PM) caveolae. (bvsalud.org)
Activity1
Regulates E3 ubiquitin-protein ligase activity of RNF19A (By similarity). (nih.gov)
Functions1
[ 24 ] Once in the intestinal tract, granule proteins are also supposed to fulfil regulatory and protective functions, for example, in host defence. (medscape.com)