CISH or cish may refer to: Chromogenic in situ hybridization, a technique in molecular biology CISH (gene), coding for the cytokine-inducible SH2-containing protein cish (mathematics), a hyperbolic function in mathematics International Committee of Historical Sciences, also referred to as Comité International des Sciences Historiques (CISH) outside the anglosphere This disambiguation page lists articles associated with the title CISH. (wikipedia.org)
The initial and important step of FISH, CISH and ISH is to prepare your tissue for hybridization, before adding probe. (ahdiagnostics.dk)
Chromogenic in situ hybridization (CISH) is a recently introduced method, and although it makes use of the in situ hybridization technology of FISH, it also takes advantage of the chromogenic signal detection of IHC that can be detected with the ordinary light microscope and costs one-quarter as much as FISH. (biomedcentral.com)
Four candidate oncogenes were validated for DNA copy number and mRNA and protein expression by quantitative polymerase chain reaction (qPCR), chromogenic in situ hybridization (CISH), reverse transcriptase-qPCR (RT-qPCR), and immunohistochemistry (IHC) in more patients. (biomedcentral.com)
Chromogenic1
Whether you select to visualize your RNA and DNA targets by fluorescence (FISH) or chromogenic (CISH) detection, you can be sure to find the best products and guidance for your workflow with our specialists. (ahdiagnostics.dk)
Natural cytotoxicity1
We show that CISH does not only regulate interleukin-15 (IL-15) signaling pathways but also natural cytotoxicity receptors (NCR) pathways, triggering CISH protein expression. (bmj.com)
Protein2
Here, we targeted an intracellular inhibiting protein 'cytokine inducible SH2-containing protein' (CISH) in NK cells to evaluate the impact on their functions and antitumor properties. (bmj.com)
Together, these data demonstrate that the intra-cellular immune checkpoint protein CISH can potentially enhance anti48 tumor responses against a broad range of cancer types regardless of PD-L1 biomarker status. (packgene.com)
Gene3
Global gene expression of Cish fl/fl Ncr1 Ki/+ NK cells compared with Cish +/+ Ncr1 Ki/+ NK cells revealed upregulation of pathways and genes associated with NK cell cycling and activation. (bmj.com)
Herein, we employed multiplex CRISPR editing of primary human T cells to systematically compare the function of CISH deletion relative to PDCD1 (the gene encoding PD-1) and/or VSIG9 (the gene encoding TIGIT) in a model of neoantigen41 mediated cancer cell cytolysis. (packgene.com)
CISH is potentially able to detect Her-2/ neu gene amplification and to minimize, if not eliminate, the false positive fraction with the IHC procedure. (biomedcentral.com)
Tumor6
Cish fl/fl Ncr1 Ki/+ NK cells display lower activation thresholds and Cish fl/fl Ncr1 Ki/+ mice are more resistant to tumor metastasis and to primary breast cancer growth. (bmj.com)
CISH deletion favors NK cell accumulation to the primary tumor, optimizes NK cell killing properties and decreases TIGIT immune checkpoint receptor expression, limiting NK cell exhaustion. (bmj.com)
In contrast, CISH inactivation enhanced antigen-specific cytolysis of tumor cells regardless of PD-L1 expression, including outperforming PD-1 and TIGIT disruption even in the presence of high PD-L1 tumor cells. (packgene.com)
Furthermore, we observed a synergistic increase in tumor cell killing when CISH and PD-1 or TIGIT are inactivated in combination, supporting the notion that these immune checkpointsregulate non-redundant pathways of T cell activation. (packgene.com)
We also compared age at diagnosis and tumor histologic grade with IHC and CISH Her-2/ neu . (biomedcentral.com)
Similarly, although the small group of well-differentiated tumors was apparently Her-2/ neu negative in both tests, no significant association was noted between any tumor histologic grade and either IHC or CISH results. (biomedcentral.com)
Deletion1
In human NK cells, CISH deletion also favors NCR signaling and antitumor functions. (bmj.com)
The direct link for online application portal is available at CISH website as per the CISH vacancy notification, these vacancies are assigned to Facilitator, Young Professional and post wise vacancy details are given here. (sarkariexamhub.in)
Published September 22, 2017 at 80 × 80 in CISH Recruitment - Junior Research Assistant Vacancy - Walk In Interview 07 October 2017 . (aisarkarijobs.in)
Methods2
To help you get the most out of your FISH, CISH, and ISH, our product specialists are trained and experienced in the methods themselves. (ahdiagnostics.dk)
Methods To further understand CISH functions in NK cells, we developed a conditional Cish-deficient mouse model in NK cells ( Cish fl/fl Ncr1 Ki/+ ). (bmj.com)
Find the right solutions for every step of your workflow in FISH, CISH and ISH - from the initial sample preparation to detection. (ahdiagnostics.dk)
Immune1
CISH is a member of a new class of intra-cellular immune checkpoint molecules that function downstream of the T cell receptor to regulate antigen-specific effector functions, including reactivity to cancer neoantigens. (packgene.com)
HUMAN2
Finally, we targeted CISH in human NK-92 or primary NK cells, using a technology combining the CRISPR(i)-dCas9 tool with a new lentiviral pseudotype. (bmj.com)
Finally, using CRISPRi, we then targeted CISH in human NK-92 or primary NK cells. (bmj.com)
Step1
Conclusion This study represents a crucial step in the mechanistic understanding and safety of Cish targeting to unleash NK cell antitumor function in solid tumors. (bmj.com)
Analysis1
In addition, CISH analysis of patients indicated that copy number amplification indeed occurred for ARHGAP19 and ZNF322A genes in lung cancer patients. (biomedcentral.com)
Applications1
CISH Applications is invited from CISH in prescribed form through online mode only for various posts. (sarkariexamhub.in)
Perform1
We were able to perform and evaluate CISH successfully on all cases. (biomedcentral.com)
Jobs1
CISH Jobs 2023 CISH will recruit the candidates on central Government Basis. (sarkariexamhub.in)
Cells2
Results In Cish fl/fl Ncr1 Ki/+ mice, we detected no developmental or homeostatic difference in NK cells. (bmj.com)
Primed Cish fl/fl Ncr1 Ki/+ NK cells display increased activation upon NCR stimulation. (bmj.com)
We assessed Her-2/ neu alteration using CISH on formalin-fixed paraffin-embedded primary invasive ductal carcinoma tumors in which IHC (CB11 antibody) had previously been performed, and we compared the results with IHC. (biomedcentral.com)
Although we noted Her-2/ neu positivity more in premenopausal women, the age at diagnosis was not significantly associated with IHC or CISH results. (biomedcentral.com)
Those cases with 2+ IHC results will be triaged and subjected to CISH. (biomedcentral.com)
Cytokine-inducible1
Cytokine-inducible SRC homology 2 domain protein (CISH) is a suppressor of cytokine signaling that controls interleukin-2 signaling pathway. (nih.gov)
HER23
Cross tabulation of HER2 gene status for CISH versus Dako FISH. (medscape.com)
Mean HER2 /CEN-17 ratio and signal numbers for all specimens with a HER2 /CEN-17 CISH ratio below 3. (medscape.com)
Microarray-based readout of ER, PR, and HER2 shows a high concordance with immunohistochemistry/CISH and provides an additional, objective, and quantitative assessment of tumor receptor status in breast cancer. (nih.gov)
Susceptibility2
Association between CISH polymorphisms and susceptibility to chronic hepatitis B in Chinese Han population]. (nih.gov)
Previously, we demonstrated Suppressor of Cytokine Signaling (SOCS) family member Cish as a promising targetable molecule to improve inhibited anti-tumor T cells. (nih.gov)
Genetic1
Genetic deletion of CISH using this new technology could result in significant increase of cytotoxicity, IFN- γ production, and IL-2 production, indicators of tumor-specific T cells properties. (nih.gov)
Human3
Western blot analysis of extracts from NK-92 cells, untreated (-) or treated (+) with Human Interleukin-2 (hIL-2) #8907 (10 ng/ml, overnight), or BaF3 cells, untreated (-) or treated (+) with Mouse Interleukin-3 (mIL-3) #8923 (10 ng/ml, 6 hr), using CISH (D4D9) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (cellsignal.com)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human CISH (hCISH, +), using CISH (D4D9) Rabbit mAb. (cellsignal.com)
Furthermore, we are attempting to genetically delete CISH in human T cells by using a lentiviral based Crispr/Cas9 technology. (nih.gov)
Research2
Nuoret tutkijat kaukana idässä : raportti Conferences of International Committee for Historical Sciences (ICHS/CISH) and International Federation for Research in Women's History (IFRWH) Jinanissa, Kiinassa 23. (jyu.fi)
Shimla and initiated biotechnology research at CISH, Lucknow. (bagchee.com)
Presentation1
Schematic presentation of the grid-based cover slip that was implemented in CISH slide screening-mapping. (iiarjournals.org)