• The detection of CTCs in peripheral blood is one of the most promising approaches to identify disseminated disease in colorectal cancer (CRC). (researchsquare.com)
  • The isolation and molecular analysis of CTCs in peripheral blood is one of the most promising approaches to identify disseminated disease, in order to upgrade or downgrade the multimodal therapy. (researchsquare.com)
  • Background: Circulating tumour cells (CTCs) are cells that have detached from a primary tumour, circulate in the peripheral blood, and are considered to be the main root of distant metastases. (iiarjournals.org)
  • Even then he presumed that these cells, today known as circulating tumour cells (CTCs), could play a role in the development of distant metastases. (iiarjournals.org)
  • Nowadays, a huge body of literature describes CTCs as cells that, after detaching from the primary tumour and circulating through the blood or lymphatic system, are the main cause of the occurrence of metastases in patients with epithelial cancer ( 2 - 4 ). (iiarjournals.org)
  • Numerous studies suggest that detection of CTCs in the blood or disseminated tumour cells (DTCs) in the bone marrow of patients with cancer is linked to poor outcomes of the disease and reduced treatment efficiency ( 5 , 6 ). (iiarjournals.org)
  • There are several different methods for the detection of DTCs and CTCs from bone marrow or blood ( 7 - 9 ). (iiarjournals.org)
  • Currently, only one methodology for the discovery and enumeration of CTCs is approved, at least for the metastatic setting by the US Food and Drug Administration (FDA), namely the so-called Cell Search® System ( 10 ). (iiarjournals.org)
  • This method is based on the immunomagnetic enrichment of CTCs from patient's blood samples, with subsequent staining of these cells. (iiarjournals.org)
  • The enumeration of circulating tumor cells (CTCs) represents an effective prognostic and predictive biomarker, which is able to monitor efficacy of adjuvant therapies, detect early development of (micro)metastases and at last, assess therapeutic responses of advanced disease earlier than traditional imaging methods. (amegroups.org)
  • Moreover, since repeated tissue biopsies are invasive, costly and not always feasible, the assessment of tumor characteristics on CTCs, by a peripheral blood sample as a 'liquid biopsy', represents an attractive opportunity. (amegroups.org)
  • In the last decade, the detection of disseminated tumor cells (DTCs) in the bone marrow and circulating tumor cells (CTCs) in the blood have demonstrated to provide useful information for the clinical management of BC by predicting treatment benefit earlier than traditional imaging methods. (amegroups.org)
  • An emerging method to detect metastasis is the analysis of circulating tumor cells (CTCs), which has shown promise in filling the gaps left by other diagnostic methods. (iiarjournals.org)
  • CTCs are tumor cells originating from primary sites or metastases that circulate in the patients' bloodstream and are very rarely found in healthy individuals ( 9 , 10 ) ( Figure 1 ). (iiarjournals.org)
  • Morphological-based isolation separates CTCs according to size discrepancies, using isolation by size of epithelial tumor cells (ISET) or according to density, using density-gradient separation ( 4 ). (iiarjournals.org)
  • Circulating tumor cells (CTCs), as one of the materials of "liquid biopsy, " have important guiding roles in the early diagnosis of head and neck squamous cell carcinoma, judgment of prognosis and therapeutic response, and monitoring of tumor recurrence and metastasis. (sdu.edu.cn)
  • focus on circulating tumor cells (CTCs)[J]. Oral Oncol, 2017, 74: 83- 89. (sdu.edu.cn)
  • Evaluation of incidence, significance, and prognostic role of circulating tumor microemboli and transforming growth factor-β receptor I in head and neck cancer[J]. Head Neck, 2017, 39 (11): 2283- 2292. (sdu.edu.cn)
  • Liquid biopsy: an emerging prognostic and predictive tool in head and neck squamous cell carcinoma (HNSCC). (sdu.edu.cn)
  • Because tumour dormancy provides a unique therapeutic window to prevent metastatic disease, we discuss on-going clinical trials and weigh the potential for immunotherapy to eradicate dormant disease. (nature.com)
  • More recently, other potential blood-based markers are emerging as independent parameters for prediction development and outcome in metastatic disease, including circulating tumor microemboli and circulating tumor materials (CTMat). (amegroups.org)
  • This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)
  • Epithelial cells in the peripheral blood of patients with cancer of the head and neck: incidence, detection and possible clinical significance[J]. Radiother Oncol, 2001, 59 (2): 213- 217. (sdu.edu.cn)
  • A simple multicolor flow cytometry protocol for detection and molecular characterization of circulating tumor cells in epithelial cancers[J]. Cytometry A, 2012, 81 (6): 489- 495. (sdu.edu.cn)
  • CD4 + T cells contribute to the remodeling of the microenvironment required for sustained tumor regression upon oncogene inactivation. (nature.com)
  • Reports of spontaneous regression and tumour infiltration with T-lymphocytes makes melanoma candidate for immunotherapies. (mdpi.com)
  • Emerging techniques using the analysis of circulating tumor cells show promising results in predicting and identifying the early stages of breast cancer metastasis in patients. (iiarjournals.org)
  • The majority of deaths from breast cancer are not due to the primary tumor itself, but are the result of metastasis to other organs in the body ( 2 ). (iiarjournals.org)
  • Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)
  • According to the American Society of Clinical Oncology (ANCO) guidelines on breast cancer follow-up and management, symptoms of breast cancer recurrence include presence of new breast lumps, pain in the bone, chest or abdomen, dyspnea and constant headaches ( 5 ). (iiarjournals.org)
  • Relationship between hematogenous tumor cell dissemination and cellular immunity in DCIS patients. (nature.com)
  • The dissemination of tumor cells from the primary tumor to distant sites through bloodstream cannot be early detected by standard imaging methods. (amegroups.org)
  • Detection of rare disseminated tumor cells identifies head and neck cancer patients at risk of treatment failure[J]. Clin Cancer Res, 2003, 9 (14): 5287- 5294. (sdu.edu.cn)
  • Significance of circulating tumor cell detection using the CellSearch system in patients with locally advanced head and neck squamous cell carcinoma[J]. Eur Arch Otorhinolaryngol, 2013, 270 (10): 2745- 2749. (sdu.edu.cn)
  • Persistence of solitary mammary carcinoma cells in a secondary site: a possible contributor to dormancy. (nature.com)
  • RESULTS: We discovered that in prostate cancer patients from The Cancer Genome Atlas (TCGA) data set, high SNHG1 expression in localized tumors correlated with reduced progression-free survival, and in a data set of both primary and metastatic tumors, high SNHG1 expression was associated with metastatic tumors. (bvsalud.org)
  • Conclusion: By this methodology, cells from mammary carcinoma cell lines can be detected in blood samples. (iiarjournals.org)
  • Goss, P. E. & Chambers, A. F. Does tumour dormancy offer a therapeutic target? (nature.com)
  • Folkman, J. Tumor angiogenesis: therapeutic implications. (nature.com)
  • Progress in clinical research on circulating tumor cells in squamous cell carcinoma of the head and neck[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 18-24. (sdu.edu.cn)
  • 6 ) emphasized that the inclusion of serum tumor markers is an important factor in the postoperative monitoring of breast cancer patients ( 7 , 8 ). (iiarjournals.org)
  • Materials and Methods: Blood samples of a healthy donor were mixed with specific numbers of cells from different breast carcinoma cell line cells. (iiarjournals.org)
  • In this regard, we used blood samples from a healthy donor mixed with different numbers of established breast cancer cells (CAMA-1, MCF7, ZR-75-1) and carried out subsequent gene expression analysis for cytokeratins 8, 18 and 19. (iiarjournals.org)
  • The primary cause of tumor-related death in breast cancer (BC) is still represented by distant metastasization. (amegroups.org)
  • Main Outcomes and Measures: The primary outcome was the frequency of actionable molecular data, defined as the presence of mismatch repair deficiency (MMRD) or high microsatellite instability (MSI-H), homologous recombination repair deficiency, or tumor mutational burden of 10 mutations per megabase or greater. (bvsalud.org)
  • In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
  • Dormant cancer cells: programmed quiescence, senescence, or both? (nature.com)
  • BACKGROUND: A majority of prostate cancer cells are in a non-proliferating, G0 (quiescent) phase of the cell cycle and may lie dormant for years before activation into a proliferative, rapidly progressing, disease phase. (bvsalud.org)
  • Additionally, imaging methods such as bone scintigraphy, liver echography and chest X-ray are to be performed bianually. (iiarjournals.org)
  • Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)
  • Klein, G. Foulds' dangerous idea revisited: the multistep development of tumors 40 years later. (nature.com)
  • In this study, we investigated the role of a long non-coding RNA (lncRNA), SNHG1, on cell proliferation, quiescence, and sensitivity to docetaxel as a potential factor important in prostate cancer biology. (bvsalud.org)
  • Circulating tumour cells in locally advanced head and neck cancer: preliminary report about their possible role in predicting response to non-surgical treatment and survival[J]. Eur J Cancer, 2012, 48 (16): 3019- 3026. (sdu.edu.cn)
  • As early as 1869, cells with the morphology of cancer cells had been found by Thomas Ashworth in the blood of a person who died from metastatic cancer ( 1 ). (iiarjournals.org)
  • Results: Cytokeratin gene expression increased in all samples, when as few as 10 tumour cells were added. (iiarjournals.org)
  • Monitoring of circulating tumor cells and their expression of EGFR/phospho-EGFR during combined radiotherapy regimens in locally advanced squamous cell carcinoma of the head and neck[J]. Int J Radiat Oncol Biol Phys, 2012, 83 (5): e685- e690. (sdu.edu.cn)
  • Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
  • Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)
  • RNAi and prostate cancer cell lines were utilized to investigate SNHG1 in vitro. (bvsalud.org)