Circulating cell derived microparticles by flow cytometry cell derived microparticles. Medical search. Frequent questions

WJMSC-derived small extracellular vesicle enhance T cell suppression through PD-L1. (nature.com)
Microparticles (MPs) are a type of extracellular vesicle (EV) distinct from exosomes (which are smaller and generated inside vesicular bodies prior to secretion through the open cannicular system) and apoptotic bodies (larger fragments of late-stage apoptotic cells carrying fragmented DNA and protein). (unityhealth.to)

Upon activation, platelets release plasma-membrane derived microparticles (PMPs) exposing phosphatidylserine (PS) on their surface. (lu.se)
This phenomenon occurs in otherwise unmanipulated murine marrow in vivo, resulting in circulating platelets that bear membrane from non-megakaryocytic hematopoietic donors. (elifesciences.org)
These findings identify emperipolesis as a new cell-in-cell interaction that enables neutrophils and potentially other cells passing through the megakaryocyte cytoplasm to modulate the production and membrane content of platelets. (elifesciences.org)
Microparticles/Microvessicles (~100-1000nm) are one of the more commonly studied populations and are typically induced following activation or inflammatory stimuli from many cell types including platelets, macrophages/monocytes, T-cells, B-cells, progenitor cells (EPCs, MSCs, etc.), cancer cells, endothelial and epithelial cells among many others. (unityhealth.to)
Bombeli,T., Schwartz,B.R., and Harlan,J.M. (1998): Adhesion of Activated Platelets to Endothelial Cells: Evidence for a GPIIb/IIIa-dependent Bridging Mechanism and Novel Roles for Endothelial Intercellular Adhesion Molecule 1 (ICAM-1), α V β 3 -Integrin and GPIbα. (9dok.net)
Chen,M. and Geng,J.G. (2006): P-Selectin mediates adhesion of leukocytes, platelets and cancer cells in inflammation, thrombosis, cancer growth and metastasis. (9dok.net)

Microparticles are plasma membrane vesicle fragments (between 0.1 and 1 μm in size) released from various cell types during activation by agonists or physical or chemical stress, including apoptosis [ 6 , 7 ]. (ersjournals.com)
Exosomes can transfer molecules from one cell to another via membrane vesicle trafficking, thereby influencing the immune system, such as dendritic cells and B cells, and may play a functional role in mediating adaptive immune responses to pathogens and tumors. (wikipedia.org)
On the other hand, the "exosome vesicle " is an extracellular particle released from the endosomal compartment of most eukaryotic cells. (frontiersin.org)

The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
Over the last decade, significant investigation in the MM field has demonstrated that the interaction of malignant PCs with BM stromal cells within the microenvironment is essential for survival of the PC clone [ 5 , 6 ]. (oncotarget.com)
Human amniotic membrane and amniotic membrane-derived mesenchymal stromal cells (hAMSCs) have produced promising results in regenerative medicine, especially for the treatment of inflammatory-based diseases and for different injuries including those in the orthopedic field such as tendon disorders. (mdpi.com)
Mesenchymal stem/stromal cells (MSCs) are increasingly used as an intravenously applied cellular therapeutic. (issca.us)
Later, Arnold Caplan's group described mesenchymal stem/stromal cells (MSCs) as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies [2-4]. (issca.us)
This concept started from the observation that bone marrow transplantation can provide stromal cells able to synthesize intact collagen type I, replacing deficient patient cell function and ameliorating disease symptoms [5]. (issca.us)

2 Upon inflammation, the pool of resident macrophages gets quickly replaced by macrophages derived from circulating monocytes. (haematologica.org)
Monocytes and lymphocytes were analysed by flow cytometry for HSP72 expression from blood samples prior to, mid-way and at the completion of the programme. (worktribe.com)

Tumor-associated B7-H1 promotes T-cell apoptosis: a potential mechanism of immune evasion. (nature.com)
There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
Plasma membrane-derived vesicles (PMVs) are described as sub-cellular vesicles released upon shear stress, cell activation, injury or apoptosis. (symbiosisonlinepublishing.com)
They are released when cells undergo activation/apoptosis via blebbing and shedding and have a function in intercellular communication. (symbiosisonlinepublishing.com)
The review focuses on the role of APC-derived EVs in regulating the transformation of macrophage phenotype, shaping foam cells, driving autophagy and/or inhibiting apoptosis of Th4 + cells, T regulatory cells, endothelial and smooth muscle cells (SMCs), as well as in facilitating oxidative stress in vasculature. (bmrat.org)

Background: Microparticles (MPs) are small vesicles (0.1-1 micron) deriving from plasma membrane budding during homeostasis and cell activation. (unibo.it)
MPs are extremely small vesicles generated via off-budding from a parent cell. (unityhealth.to)

In this study, our goals were to determine if MM cells release MVs, and if so, begin to characterize their biologic activity. (oncotarget.com)

Myelofibrosis (MF) is a clonal neoplasia of the hematopoietic stem/progenitor cells characterized by distinctive abnormalities in megakaryocyte (MKC) development and platelet (PLT) activation. (unibo.it)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
They are secreted by a wide array of cells, including cardiac myocytes, mature and progenitor endothelial cells, mesenchymal stem cells, immune cells like antigen-presenting cells (APCs), and malignant cells 5 , 6 . (bmrat.org)

Levels of platelet-derived and leukocyte-derived microparticles were also significantly higher in patients with OSA (2,267 (1,102-3,592) microL(-1) and 20 (14-31) microL(-1), respectively) compared with control subjects (925 (328-2,068) microL(-1) and 15 (5-23) microL(-1), respectively). (ox.ac.uk)
In patients with minimally symptomatic obstructive sleep apnoea, levels of AnnexinV-positive, platelet- and leukocyte-derived microparticles are elevated when compared with closely matched control subjects without obstructive sleep apnoea. (ox.ac.uk)
A significant increase in presumably mostly leukocyte-derived CD31+/CD41− MV (1.32 (1.09-1.93)-fold [median (25th-75th quartiles)], p = 0.021) was observed post- to prefilter, whereas platelet-derived MV as well as AnnexinV-binding MV were unaltered. (springeropen.com)

As circulating PMPs-levels were not altered in Gas6-/- mice compared to Gas6+/+ mice, we hypothesize that the Gas6-mediated uptake is not a means to clear the bulk of circulating PMPs but may serve to phagocytose PMPs locally generated at sites of platelet activation and as a way to affect endothelial responses. (lu.se)
In 57 patients with minimally symptomatic OSA, and 15 closely matched control subjects without OSA, AnnexinV-positive, platelet-, leukocyte- and endothelial cell-derived microparticles were measured by flow cytometry. (ox.ac.uk)
In patients with chronic renal failure increased systemic levels of platelet-derived as well as procoagulatory AnnexinV-binding MV were found in blood following RRT [ 13 ]. (springeropen.com)
No correlation was observed at baseline between the percentages of MK/PLT-derived MPs and platelet number, allele burden, splenomegaly and constitutional symptoms. (unibo.it)
Qin R , Zhu H , Wang F , Song M , Lin P , Xing Y , Zhang W , Zhong M , Wang Z , . Platelet activation in diabetic mice models: the role of vascular endothelial cell-derived protein disulfide isomerase-mediated GP IIb/IIIa receptor activation. (aging-us.com)
The purpose of this study was to investigate the release of Endothelial microparticle (EMP)-associated protein disulfide isomerase (PDI) after endothelial cell injury induced in diabetes and the changes in platelet activation. (aging-us.com)
Literature survey indicated the origin of the miR from liver cells and not of platelet, leukocyte, smooth muscle or endothelial (EC) origin. (biomedcentral.com)

Exosomes are membrane-bound extracellular vesicles (EVs) that are produced in the endosomal compartment of most eukaryotic cells. (wikipedia.org)
If the MVB fuses with the cell surface (the plasma membrane), these ILVs are released as exosomes. (wikipedia.org)
Since the size of exosomes is limited by that of the parent MVB, exosomes are generally thought to be smaller than most other EVs, from about 30 to 150 nanometres (nm) in diameter: around the same size as many lipoproteins but much smaller than cells. (wikipedia.org)
In the case of cancer cells, exosomes may show differences in size, shape, morphology, and canonical markers from their donor cells. (wikipedia.org)
Exosomes were first discovered in the maturing mammalian reticulocyte (immature red blood cell) by Stahl and group in 1983 and Johnstone and group in 1983 further termed 'exosomes' by Johnstone and group in 1987. (wikipedia.org)
Exosomes were shown to participate in selective removal of many plasma membrane proteins as the reticulocyte becomes a mature red blood cell (erythrocyte). (wikipedia.org)
The ILVs become exosomes if the MVB merges with the cell membrane, releasing the internal vesicles into the extracellular space. (wikipedia.org)
Exosomes contain various molecular constituents of their cell of origin, including proteins and RNA. (wikipedia.org)
Although the exosomal protein composition varies with the cell and tissue of origin, most exosomes contain an evolutionarily-conserved common set of protein molecules. (wikipedia.org)
The content of exosomes changes depending on the cells of origin, and they thereby reflect their originating cells. (wikipedia.org)
Therefore, scientists who are actively researching the role that exosomes may play in cell-to-cell signaling, often hypothesize that delivery of their cargo RNA molecules can explain biological effects. (wikipedia.org)
For example, mRNA in exosomes has been suggested to affect protein production in the recipient cell. (wikipedia.org)
However, another study has suggested that miRNAs in exosomes secreted by mesenchymal stem cells (MSC) are predominantly pre- and not mature miRNAs. (wikipedia.org)
Because the authors of this study did not find RNA-induced silencing complex-associated proteins in these exosomes, they suggested that only the pre-miRNAs, but not the mature miRNAs in MSC exosomes, have the potential to be biologically active in the recipient cells. (wikipedia.org)
The study of exosomes and other cell-derived microvesicles is an area of rapidly growing importance and the subject of intense interest and research. (malvernpanalytical.com)
2009) define exosomes as 40 -100 nm diameter membrane vesicles of endocytic origin that are released by most cell types upon fusion of multivesicular bodies with the plasma membrane, presumably as a vehicle for cell-free intercellular communication. (malvernpanalytical.com)
Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
These results provide a mechanism by which CMV can exacerbate allograft rejection and suggest a novel function of EC-derived exosomes that could contribute in a more general manner to immune surveillance. (aai.org)

Although the exact role of cellular microparticles remains partially understood, there is increasing evidence to suggest an active role for microparticles in PH pathophysiology. (ersjournals.com)

Review/Rese a: Acute oxygen sensing-Role of metabolic specifications in peripheral chemoreceptor cells. (us.es)

Patients with PH exhibited higher circulating levels of microparticles compared to control subjects and in vitro or in vivo generated microparticles can induce endothelial dysfunction, interfere with coagulation pathways or modulate inflammatory phenomenon. (ersjournals.com)

Circulating cell-derived microparticles in patients with minimally symptomatic obstructive sleep apnoea. (ox.ac.uk)
In patients with OSA, median (interquartile range) levels of AnnexinV-positive microparticles were significantly elevated compared with control subjects: 2,586 (1,566-3,964) microL(-1) versus 1,206 (474-2,501) microL(-1), respectively. (ox.ac.uk)
sEVs were isolated from different clinical samples of head and neck squamous cell carcinoma (HNSCC) patients, the level and cellular origins of PD-L1-positive sEVs were assessed. (nature.com)
PD-L1-positive sEVs in HNSCC patients had various cellular origins, including tumor cell, T cell, B cell, dendritic cell and monocyte/macrophage. (nature.com)
Tracking the evolution of circulating exosomal-PD-L1 to monitor melanoma patients. (nature.com)
Risk-adapted therapies for AML, ALL, and CLL patients consist of chemotherapy with/without stem cell transplantation (SCT), but the rate of early failures and relapses is still unsatisfying. (nature.com)
All index patients displayed positive SARS-CoV-2-specific antibody and T-cell responses that lasted up to 102 days after symptom onset. (cdc.gov)
Studies of HIV-1 infection of fractionated CD4 and CD8 T-cells from normal donors and AIDS patients have revealed that depletion of CD4 T-cells results from the ability of HIV-1 to selectively infect, replicate in, and ultimately destroy this T lymphocyte subset (Klatzmann et al. (justia.com)
We carried out a study among 120 patients divided by high-CFR and low-CFR to profile the miRNA content of circulating EVs. (biomedcentral.com)
Human CMV infection is controlled by T cell-mediated immunity and in immunosuppressed transplant patients it is associated with acute allograft rejection as well as chronic allograft vasculopathy. (aai.org)
Antwi-Baffour S, Boafo AO, Kyeremeh R, Mahmood SA (2013) Plasma Membrane-derived Vesicles (PMVs) in G6PD Deficient Patients. (symbiosisonlinepublishing.com)
There is therefore the suspicion of increased levels of Plasma Membrane-derived Vesicles (PMVs) in G6PD deficient patients. (symbiosisonlinepublishing.com)

The tyrosine kinase receptors Tyro3, Axl and Mer (TAMs) and their ligands protein S and Gas6 are involved in the uptake of PS-exposing apoptotic cells in macrophages and dendritic cells. (lu.se)
The uptake of labeled MPs in the presence of protein S and Gas6 in human aortic endothelial cells (HAEC) and human umbilical vein endothelial cells (HUVEC) was monitored by flow cytometry, western blotting and confocal/electron microscopy. (lu.se)
However, many disease-predisposing factors and/or contributing factors have been identified, including inflammation, endothelial cell dysfunction, aberrant vascular wall cell proliferation and mutations in the bone morphogenetic protein-receptor type 2 ( Bmpr2 ) gene [ 1 - 3 ]. (ersjournals.com)
EVs were characterized by transmission electron microscopy and fluorescence nanoparticle tracking analysis, and EV surface protein profiles were analyzed by multiplex bead-based flow cytometry to identify tumor- or immune system-related EVs of AML, ALL, CLL, and healthy samples. (nature.com)
It is disclosed herein that the HIV-1 envelope protein gp120 binds to α4β7 integrin on CD4+ T-cells, natural killer (NK) cells and CD8+ T-cells. (justia.com)
To analyze changes in protein expression of WJCs we performed a comparative proteomic analysis (2DE followed by MALDI-TOF MS) between fresh and post-frozen cell culturing. (fupress.net)
2003): Alternatively spliced human tissue factor: a circulating, soluble, thrombogenic protein. (9dok.net)

Furthermore, circulating microRNAs in the serum are resistant to lysis and are stable against RNase, as they are hidden in microparticles, apoptotic bodies, etc. [ 1 ]. (archivesofmedicalscience.com)

Blood and saliva samples will be analysed for markers of inflammation and immune function, using cytometric bead array and flow cytometry techniques. (bmj.com)
Flow cytometric analysis demonstrated the high purity of generated microsamples. (researchgate.net)

Dendritic cells (DC) have a special role in the production of IFN and are the main sources of serum interferon. (bmj.com)
Interestingly, MPs (containing RNA and DNA) could stimulate type I IFN production in plasmacytoid dendritic cells and subsequently the release of MPs from DC. (bmj.com)
The innate immune system includes macrophages, dendritic cells (DC), and natural killer (NK) cells, which respond quickly to an immunological threat. (nature.com)
Studies to antigen uptake, processing and presentation to T cells, the biology of dendritic cells and the regulation of immunity are aimed to raise our understanding of the immune reactions to tumor cells and improve immunotherapy of cancer. (universiteitleiden.nl)

It has been shown that hemofiltration is able to influence levels of circulating mediators such as inflammatory cytokines by adsorption or filtration [ 4 ]. (springeropen.com)
Furthermore, other cells at the interface of the innate and the adaptive immune system (e.g cytokine-induced killer cells (CIK) or invariant natural killer T-cells (iNKT)) are important mediators in antitumor-, autoimmune-, and antimicrobial responses and tumor surveillance. (nature.com)
Microparticles are increasingly becoming realized as important biomarkers and mediators of disease. (unityhealth.to)

It was verified that co-expression of CD80 disrupted the binding of sEV PD-L1 to its receptor PD-1 on T cells and attenuated the immunosuppression mediated by sEV PD-L1 both in vitro and in vivo. (nature.com)
Fig. 4: PD-L1-positive I-sEVs did not suppress T cells in vitro. (nature.com)
They are also released in vitro by cultured cells into their growth medium. (wikipedia.org)
The aim of the present work was to investigate molecular changes that occur in WJCs proteome at different culture conditions (freshly and post-frozen cell preparations) and to elucidate possible mechanism involved in maintaining active proliferation and maximal cellular plasticity in order to optimize in vitro culturing procedure. (fupress.net)
In vitro, CD4 + T cells from CMV-positive donors (but not CMV-negative donors) are readily activated by CMV-infected allogeneic EC, although it is unclear how allogeneic CMV-infected EC activate self-class II MHC-restricted memory CD4 + T cells. (aai.org)

IFN has the potential to dramatically influence the development, progression, and pathogenesis of SLE as it can influence the function and activation state of most major immune cell subsets and function as a bridge between innate and adaptive immunity. (bmj.com)
In otherwise healthy CMV-infected individuals, CMV persists in a state of clinical latency, its replication and pathogenesis kept in check by the host adaptive immune response involving both CD4 + and CD8 + T cells ( 2 , 3 ). (aai.org)

MV subtypes in blood were analyzed by high-sensitivity flow cytometry. (springeropen.com)
The difficulty is that the small particle size begins to stretch the limit of conventional cytometers and even the limits of newer high-sensitivity flow cytometers. (unityhealth.to)

Macrophages are versatile cells that can be polarized by the tissue environment to fulfill required needs. (haematologica.org)
To understand if polarization of macrophages can lead to a procoagulant macrophage subset we polarized human monocyte derived macrophages to a proinflammatory and an alternative activation state. (haematologica.org)
In conclusion, we provide evidence that proinflammatory polarization of macrophages does not lead to enhanced procoagulatory function, whereas alternative polarization of macrophages leads to an increased expression of TF and increased production of TF bearing extracellular vesicles by these cells suggesting a procoagulatory phenotype of alternatively polarized macrophages. (haematologica.org)
Macrophages are cells of the innate immune system which play numerous and vastly different functions within the body. (haematologica.org)
6 In addition, these cells are present during early wound healing and proinflammatory macrophages are characterized by a pronounced ability to degrade tissue. (haematologica.org)

These well-regulated processes to transfer information between cells occur through direct cell-cell contact, various soluble bioactive factors and through cellular microparticles [ 6 , 7 ]. (ersjournals.com)

The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
Without limitation, some embodiments comprise methods, systems, and/or compositions relating to microRNAs and/or cell based therapies and the use of the same in the research, diagnosis, or treatment of injury or disease. (justia.com)
There is a constant crosstalk between microRNAs derived from various cell sources. (archivesofmedicalscience.com)
In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)
MicroRNAs are produced by a variety of cells [ 1 - 4 ]. (archivesofmedicalscience.com)

The PD-L1 on tumor cell-derived small extracellular vesicles (sEVs) can suppress the proliferation and cytokine production of T cells. (nature.com)
It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
Of interest, MM-derived MVs were enriched with the biologically active form of CD147, a transmembrane molecule previously shown by us to be crucial for MM cell proliferation. (oncotarget.com)
In summary, this study demonstrates the significance of MV shedding and MV-mediated intercellular communication on malignant plasma cell proliferation, and identifies the role of MV-enriched CD147 in this process. (oncotarget.com)

blood cells were removed by two steps of centrifugation process (2500 g for 15 min at 20 C two time). (bmj.com)
Impact of sample processing on the measurement of circulating microparticles: storage and centrifugation parameters. (us.es)

EVs in circulation carry genetic material and proteins from their cell of origin, proteo-transcriptomic signatures that act as biomarkers. (wikipedia.org)
Aiming to provide proof-of-concept evidence and methodology for the potential role of serum-derived EVs as biomarkers in leukemic versus healthy samples in this study, we hope to pave the way for future detection of promising biomarkers for imminent disease progression and the identification of potential targets to be used in a therapeutic strategy. (nature.com)

High correlation between 2 flow cytometry platforms in the microparticles analysis using a new calibrated beads strategy. (us.es)

APC-derived EVs act as triggers of angiogenesis, neovascularization and inflammation through their participation in microvascular inflammation, angiogenesis, development of atherosclerotic plaques, and modulation of their instability. (bmrat.org)

Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)

Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
Cryopreservation is the only mode of long-term storage of viable cells and tissues for cellular therapy, stem cell transplantation and/or tissue engineering. (fupress.net)
However the freeze-thaw process strongly contributes to cell and tissue damage with several mechanism, including oxidative stress, intracellular ice formation (IIF) dependent cell injury and altered physical cellular properties, i.e. osmotic and ion homeostasis. (fupress.net)
The tissue source of the MSCs was in most cases not decisive, and cells from various tissue sources were explored. (issca.us)

PMP also differ from the microparticles derived from megakaryocytes despite the presence of several identical surface markers on the latter. (malvernpanalytical.com)

Fig. 3: Different clinical significance between tumor cell- and immunocyte-derived PD-L1-positive sEVs. (nature.com)

Microparticles from tumors exposed to radiation promote immune evasion in part by PD-L1. (nature.com)
Therapeutic vaccination has the major advantage that the induced immune response selectively attacks tumors while leaving the healthy cells untouched. (universiteitleiden.nl)

Circulating cell-derived microparticles have been shown to have pro-inflammatory, pro-coagulant and endothelial function-impairing effects, as well as to predict subclinical atherosclerosis and cardiovascular risk. (ox.ac.uk)
MPs express antigens and contain constituents from cell of origin and are increased in conditions that are characterized by high cell turnover or death, particularly inflammatory, autoimmune and neoplastic diseases. (unibo.it)
These include macrophage and smooth muscle cell (SMC) phenotypic switching and various inflammatory signaling, such as IL-33/suppression of tumorigenesis 2 (ST2), Ras-Raf-MEK-ERK pathways, and JAK-STAT signaling pathways 14 , 15 , 16 , 17 . (bmrat.org)

2013) focused on the role of cellular exocytic vesiculation in health, disease, and transfusion medicine, recognizing that microparticles (MPs), small membrane-derived vesicles which are derived from many cell types and released into the circulation under shear stress, complement activation, proapoptotic stimulation, cellular damage, or agonist interaction with cell surface receptors. (malvernpanalytical.com)
Cell-generated EV spike-in models were isolated and purified by size-exclusion chromatography, stained with cellular dyes and characterized by nano flow cytometry. (biomedcentral.com)
The observation that the immune system can also attack malignant cells has been known for many years but the cellular and molecular mechanisms have become clear in the last decades. (universiteitleiden.nl)
The loss of CD4 helper/inducer T cell function probably underlies the profound defects in cellular and humoral immunity leading to the opportunistic infections and malignancies characteristic of the acquired immunodeficiency syndrome (AIDS) (Lane et al. (justia.com)
WJCs postfrozen showed a qualitative and quantitative changes compared to cells from fresh preparation, expressing proteins involved in replication, cellular defense mechanism and metabolism, that ensure freeze-thaw survival. (fupress.net)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

The levels of PMVs in those samples were quantified using quantitative flow cytometry. (symbiosisonlinepublishing.com)

PLT (CD61+CD62P+) and MK (CD61+CD62P-)-derived MPs were analysed in PLT poor plasma samples by flow cytometry (CytoFLEX, Flow Cytometer-Beckman Coulter). (unibo.it)
In the reticulocyte, as in most mammalian cells, portions of the plasma membrane are regularly internalized as endosomes, with 50 to 180% of the plasma membrane being recycled every hour. (wikipedia.org)
As such they have a plasma membrane bilayer and biological molecule endowment derived from a parent cell. (unityhealth.to)
Since blood plasma circulates through the whole body, it is often the biofluid of choice for biomarker detection in EVs. (biomedcentral.com)
Plasma-derived EVs were captured and detected using this straightforward protocol, sequentially combining isolation and staining of specific surface markers, such as CD9 or CD41. (biomedcentral.com)
The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
Multiple myeloma (MM) is characterized by the clonal expansion of malignant plasma cells within the bone marrow. (oncotarget.com)

The spleen pinches off the Heinz body and the overlying membrane, leaving a "bite cell" or "blister cell" which eventually haemolysis. (symbiosisonlinepublishing.com)
However, some membrane alterations have been described in G6PD deficient red blood cells, such as the oxidation and clustering of membrane proteins, the binding of hemichromes to the internal face of the membrane, the destabilization of the membrane and the release of micro-vesicles [6]. (symbiosisonlinepublishing.com)

A multiplex array profiling on circulating EVs revealed mir-224-5p ( p -value ≤ 0.000001) as the most differentially expressed miRNA in the Low-CFR group and showed a significantly independent relationship to CFR. (biomedcentral.com)

Antigen-presenting cells (APCs) produce and release a broad spectrum of EVs involved in the pathogenesis of atherosclerosis. (bmrat.org)

These data suggest that exposure to SARS-CoV-2 can induce virus-specific T-cell responses without seroconversion. (cdc.gov)

Almost 50 years ago, it was observed that MKs can engulf other hematopoietic cells, most commonly neutrophils ( Larsen, 1970 ). (elifesciences.org)
The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)

However, PD-L1-positive sEVs derived from immune cells did not exert immunosuppressive functions due to the co-expression of CD80. (nature.com)
Circulating microparticles (MPs) are ubiquitous in the blood of healthy individuals, These MPs play an active role in coagulation and intercellular communication and assist in activation or suppression of the immune system, depending on their parental cell origin. (bmj.com)
Regularly, anergy of T cells or other immune cells can be regularly reverted to anti-leukemic functionality. (nature.com)
Basic research of the T cell immune system in many different aspects is central in his team. (universiteitleiden.nl)
CMV infects endothelial cells (EC) and it is thought that CMV-specific host immune responses to infected allograft EC contribute to rejection. (aai.org)
Numerous animal studies as well as observational and clinical trials have shown that atherosclerosis arises from immune activation, with several cell-type specific pathways involved. (bmrat.org)

These MVs/MPs are often characterized on the basis of cell surface antigens derived from the host cells (i.e. (unityhealth.to)
Flow cytometry is an excellent but challenging method to assess MPs, with the advantage of high-throughput analysis, the power to quantitate several surface antigens (often of low expression level due to small particle size), and the ability to identify MP population subsets that may be of low frequency (i.e. circulating endothelial MPs). (unityhealth.to)
however, 6 reported COVID-19 symptoms within a median of 7 days after their partners, and 4 of those showed a positive SARS-CoV-2-specific T-cell response against 3 or 4 SARS-CoV-2 antigens that lasted up to 93 days after symptom onset. (cdc.gov)

The primary immunologic abnormality resulting from infection by human immunodeficiency virus (HIV) is the progressive depletion and functional impairment of T lymphocytes expressing the CD4 cell surface glycoprotein. (justia.com)

Human vascular endothelial cells (EC) 3 display both class I and class II MHC molecules and are directly recognized by CD8 + and CD4 + alloreactive T cells, respectively ( 12 , 13 , 14 , 15 ). (aai.org)

Glusoe-6-phosphate dehydrogenase (G6PD) deficiency on the other hand is an x-linked enzymopathy characterized by the inability of red blood cells (RBCs) to withstand oxidative stress. (symbiosisonlinepublishing.com)

5 Functionally, proinflammatory polarization leads to potent effector cells that kill intracellular micro-organisms and tumor cells. (haematologica.org)

His research focus is on haematopoietic stem cell transplantation (HSCT) and translational research of normal and malignant stem cells. (edu.au)

Professor Ma heads the Blood, Stem Cell and Cancer Research Programme at St Vincent's Centre for Applied Medical Research, Sydney. (edu.au)
Ma DD , 2007 , 'Expression of Neurofilament Proteins in Adult Bone Marrow Mesenchymal Stem Cells and Embryonic Stem Cells and Their Clinical Potential' , in Davenport LP (ed. (edu.au)
Stem Cell Research Advances , edn. (edu.au)
Our previous proteomics investigation was carried on Wharton's jelly cells (WJCs), fibroblastlike cells with similar properties to mesenchymal stem cells, therefore a rich source of primitive cells to be potentially used in regenerative medicine. (fupress.net)

These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
MicroRNA-126 (miR-126) is endothelial cell (EC) specific and plays a key role in regulating EC function, controlling angiogenesis, and maintaining vascular integrity. (justia.com)

3) investigate whether circulating MK and PLT- MPs may be a biomarker of response to RUX. (unibo.it)
In the present work, we have isolated and characterized the extracellular vesicles from human AD-MSC to investigate their role in the chondroprotective actions of these cells. (karger.com)

We found that both endothelial cell types can phagocytose PMPs, and using TAM-blocking antibodies or siRNA knock-down of individual TAMs we show that the uptake is mediated by endothelial Axl and Gas6. (lu.se)
We investigated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies and T-cell responses against SARS-CoV-2 and human coronavirus (HCoV) 229E and OC43 in 11 SARS-CoV-2 serodiscordant couples in Strasbourg, France, in which 1 partner had evidence of mild coronavirus disease (COVID-19) and in 10 unexposed healthy controls. (cdc.gov)

In hematological malignancies, tumor-derived EVs might reprogram the bone marrow environment, suppress antileukemic immunity, mediate drug resistance and interfere with immunotherapies. (nature.com)
In the 1970s, Friedenstein and colleagues [1] first reported that locally applied culture-expanded populations of bone marrow stroma-derived fibroblastic cells remained at their injection sites under the kidney capsule, where an ectopic hematopoiesis was initiated. (issca.us)
Pivotal studies by the group of Horwitz in children with osteogenesis imperfecta, an inherited enzyme deficiency of collagen synthesis by mesenchymal cells in bone, opened the field for intravenous use of MSCs. (issca.us)
Engraftment of donor-type MSC-derived osteoblasts was shown using bone specimens and microsatellite DNA marker analysis. (issca.us)

On the other hand, the number of circulating hEPC is increased in hypoxia or acute myocardial infarction (AMI). (intechopen.com)

Changes in the concentration and/or composition of circulating Mps have been described in various autoimmune diseases, including rheumatoid arthritis (RA) systemic sclerosis (SSc) and SLE. (bmj.com)

Rates of complete remission (CR), prognosis and survival depend on the grade of anemia, thrombocytopenia, white blood cell expansion and karyotypes. (nature.com)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
Blood collection is easy and minimally invasive, yet reproducible procedures to obtain pure EV samples from circulating biofluids are still lacking. (biomedcentral.com)
Here, we detail and model a blood separation principle which exploits discrete viscosity differences caused by blood particle sedimentation in a laminar flow. (researchgate.net)
It is measured as the ratio of coronary blood flow at its maximum during adenosine infusion to when at rest. (biomedcentral.com)
Abnormal adhesion of red blood cells in polycythemia vera: a prothrombotic effect? (labex-grex.com)
however, the cells were trackable in blood only. (issca.us)
There is an increased level of PMVs in the circulating blood of G6PD deficient individuals as compared to G6PD normal individuals. (symbiosisonlinepublishing.com)
Red blood cells (RBCs) require constant energy to maintain their biconcave disc shape and haemoglobin in reduced form [1]. (symbiosisonlinepublishing.com)

Anatomy40

Organisms4

Diseases6

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment61

Phenomena and Processes46

Disciplines and Occupations8

Technology, Industry, Agriculture22

Health Care3

Extracellular vesicle2

Platelets6

Vesicle3

Stromal cells7

Monocytes2

Apoptosis5

Small vesicles2

Characterize1

Progenitor4

Leukocyte-derived3

Platelet7

Exosomes18

Pathophysiology1

Peripheral1

Endothelial dysfunction1

Patients12

Protein7

Apoptotic1

Cytometric2

Dendritic cells4

Mediators3

Vitro5

Adaptive2

High-sensitivity flow2

Macrophages5

Soluble1

MicroRNAs5

Proliferation5

Centrifugation2

Biomarkers2

Platforms1

Inflammation1

Cytokines1

Tissue4

Megakaryocytes1

Tumor cell1

Tumors2

Inflammatory3

Cellular6

Quantitative1

Plasma7

Membrane2

Multiplex1

Pathogenesis1

Induce1

Hematopoietic2

Immune6

Antigens3

Lymphocytes1

Vascular endothelial cells1

Oxidative stress1

Intracellular1

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Stem4

Angiogenesis3

Investigate2

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Bone4

Hypoxia1

Systemic sclerosis1

Blood9