• Nutraculture™ Phycocyanin™ induces apoptosis in the existing and proliferating cancer cells and being a natural antioxidant, it also helps getting rid of any cancer promoting oxidative stress. (nutraculture.com)
  • It is established that Phycocyanin induces apoptosis in cancer cells by changing the Bcl-2/Bax ratio (Bcl-2 is an anti apoptotic protein, Bax is a pro-apoptotic protein, the ratio of Bcl-2/Bax represents the degree of apoptosis) and the release of Cyt-c in the cytosol (Lu et. (nutraculture.com)
  • et al, The Flavagline Compound 1-(2-(dimethylamino)acetyl)-Rocaglaol Induces Apoptosis in K562 Cells by Regulating the PI3K/ Akt/mTOR, JAK2/STAT3, and MAPK Pathways. (sabbiotech.cn)
  • DNA (cytosine-5)-methyltransferase 3A (DNMT3A)-mutated acute myeloid leukemia (AML) has a poor prognosis, but the exact mechanism is still unclear. (frontiersin.org)
  • Acute myeloid leukemia (AML) is a common, heterogeneous, and aggressive hematopoietic malignancy, characterized by apparent genetic abnormalities ( 1 ). (frontiersin.org)
  • Although the disease can evolve toward acute leukemia, morbidity and mortality most frequently result from a marrow failure syndrome. (acgtfoundation.org)
  • In early phase clinical trials for both acute and chronic myeloid leukemias, we have observed the induction of anti-tumor immunity and associated clinical responses following K562/GM-CSF vaccination (see preliminary data). (acgtfoundation.org)
  • We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
  • established from the bone marrow of a 12-year-old boy with acute lymphoblastic leukemia (cALL) at relapse in 1979. (maayanlab.cloud)
  • Human acute myeloid leukemia established from the peripheral blood of a 35-year-old woman with acute myeloid leukemia (AML FAB M2) in 1976. (maayanlab.cloud)
  • q11)) is the hallmark of chronic myelogenous leukemia (CML) but is also present in 15-30% of adult B-cell acute lymphoblastic. (karger.com)
  • The growth of these leukemic cells are controlled by either initiating cell differentiation or apoptosis to occur. (wikipedia.org)
  • These changes can also drive the leukemic cells to a state of stress, which allows for increased sensitivity of the cells to drugs that initiate apoptosis. (wikipedia.org)
  • Holyoake, T.L. A comparison of normal and leukemic stem cell biology in chronic myeloid leukemia. (eurekaselect.com)
  • Karyotype analysis indicates novel chromosomes and gene expression analysis suggests a shift of cultured K562 cells away from patient-derived leukemic cells. (bvsalud.org)
  • Moreover, T2CA induced the activation of functional NK cells in human lymphocyte cultures that in turn contributed to the death of K562 leukemic cells in a dose-dependent manner in co-culture experiments. (arjournals.org)
  • The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
  • Growth of cancer cells is regulated by the balance between cell proliferation and apoptosis, which is a normal physiological process serving to eliminate unwanted cells and maintain homeostasis in healthy tissue. (nutraculture.com)
  • We found that the expanded cells possessed significantly improved immune effector functions, including proliferation, differentiation, and cancer cell killing, both in vitro and in the humanized mouse model. (nature.com)
  • Modified pectin (MP) is a bioactive complex polysaccharide that is broken down into smaller fragments of units and used as an oral dietary supplement for cell proliferation. (encyclopedia.pub)
  • et al, CD155 Cooperates with PD-1/PD-L1 to Promote Proliferation of Esophageal Squamous Cancer Cells via PI3K/Akt and MAPK Signaling Pathways. (sabbiotech.cn)
  • The myelodysplastic syndromes (MDS) are a group of clonal neoplastic hematologic disorders characterized by varying degrees of bone marrow failure, abnormal hematopoiesis, and proliferation of myeloid blast cells. (acgtfoundation.org)
  • Bosutinib is a potent inhibitor of CML cell proliferation in vitro and has demonstrated promising activity in CML patients resistant or intolerant to IM as well as in newly diagnosed patients with chronic phase CML (CML-CP). (mdm2-receptor.com)
  • Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis. (wikipedia.org)
  • Cell differentiation is induced by the deacetylase activity in these "undifferentiated progenitor cells," which alters the phenotype and morphology of the K562 cells. (wikipedia.org)
  • This gene targets the cyclin-dependent kinase inhibitor, p21, and causes cell differentiation, cell cycle arrest in G1, and ultimately apoptosis. (wikipedia.org)
  • Other methods being focused on in the regulation of K562 cells include therapeutic methods like polyphyllin D, which caused differentiation from the progenitor state to occur, and for apoptosis to begin. (wikipedia.org)
  • Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
  • Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
  • Cancer is the uncontrolled growth of cells in the body, which is caused by the stepwise accumulation of mutations that affect cell growth control, differentiation and survival. (nutraculture.com)
  • Inevitably, there are fundamental and applied questions, such as those relating to transcriptional control of stem cell differentiation, intrinsic noise in gene expression, and the origins of disease, that may only be addressed at the single cell level. (justia.com)
  • Background: The molecular mechanisms of differentiation of intestinal epithelial cells, is poorly understood and disruption of this balance may result in neoplastic transformation and malignant growth. (metu.edu.tr)
  • The family of CCAAT/Enhancer Binding Protein (C/EBP) transcription factors is implicated in cellular growth, differentiation, inflammation and development and control differentiation in several cell types. (metu.edu.tr)
  • In this proposal, we seek to evaluate the integration of K562/GM-CSF vaccination with systemic therapies for MDS that alter host immunity and/or hematopoietic cellular differentiation. (acgtfoundation.org)
  • Here we demonstrate that C-terminal-truncating Asxl1 mutations (ASXL1-MTs) inhibited myeloid differentiation and induced MDS-like disease in mice. (jci.org)
  • miR-125a reduced expression of C-type lectin domain family 5, member a ( Clec5a ), which is involved in myeloid differentiation. (jci.org)
  • ASXL1 mutations inhibit G-CSF-induced myeloid differentiation of 32Dcl3 cells. (jci.org)
  • F ) Surface expression of CD11b in 32Dcl3 cells transduced with indicated plasmid after incubation with 1 ng/ml IL-3 (blue) or 50 ng/ml G-CSF for 6 days (red) was analyzed by flow cytometry (top). (jci.org)
  • Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
  • Chronic myeloid leukemia (CML), an acquired malignant myeloproliferative disorder of hematopoietic stem cells, is one of the three most common forms of leukemia. (edu.hk)
  • Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. (nkmaxbio.com)
  • Thought to be disorders of hematopoietic stem cells, clonal cytogenetic abnormalities are frequently identified. (acgtfoundation.org)
  • Evidence exists that immune activation against hematopoietic elements frequently occurs in MDS patients, based on the identification of lymphocytic infiltrates in the marrow, oligoclonal expansion of T cells, and excessive production of tumor necrosis factor alpha. (acgtfoundation.org)
  • In conclusion, the data shown here demonstrated that CHMFL-074 was impressive against both unchanged CML cell lines and BCR-ABL positive individual major hematopoietic cells. (molecularcircuit.com)
  • Nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOXs) are a major generator of ROS in leukemia, and marine natural products have shown promising activities for the treatment of hematopoietic malignancies. (unina.it)
  • Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
  • This clinical translational proposal aimed to assess the therapeutic potential of genetically targeted T cells in the treatment of leukemia and lymphoma. (acgtfoundation.org)
  • Importantly, he found that T cell stimulation with the cytokine lnterleukln-15, but not the widely used lnterteukin-2, is critical for sustaining the therapeutic activity of infused CO19-spedflc T cells. (acgtfoundation.org)
  • Valencia J, Gul-Uludağ, H, Mahdipoor P. Jiang, X, Uludağ H. Investigating siRNA delivery to chronic myeloid leukemia K562 cells with lipophilic polymers for therapeutic BCR-ABL down-regulation. (udea.edu.co)
  • In this study, therapeutic effects of drug-conjugated nanocarrier system with enhanced stability and double moiety pH-sensitivity on breast cancer (SKBR-3- HER-2- positive), normal breast epithelial (MCF-10A, HER-2-negative) and chronic myeloid leukemia (K562, HER-2-negative) cells were determined. (iyte.edu.tr)
  • In this study, we sought to explore whether ivermectin (IVM), a macrolide antiparasitic agent, could overcome the resistance of cancer cells to the therapeutic drugs. (alecomm.com)
  • K562 cells are part of the NCI-60 cancer cell line panel used by the National Cancer Institute. (wikipedia.org)
  • Another important group of regulators of the K562 line are Sirtuins, referred to as SIRTS. (wikipedia.org)
  • The Leukemia-Lymphoma Cell Line Factsbook. (wikipedia.org)
  • BaF3 mouse pro-B cell line treated with 10 ng/mL Recombinant Mouse IL-3 (Catalog # 403-ML ) for 1 hour was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. (rndsystems.com)
  • SP1 and GATA3) and HDAC2-GATA1 regulatory networks on rescued regions in K562 cell line. (biomedcentral.com)
  • 50% of all common SNPs in a hepatocarcinoma and a myeloid leukemia cell line. (biologists.com)
  • In the present study, we tested whether CX-5461 could potentiate the effect of imatinib in the human chronic myeloid leukemia cell line K562. (mendeley.com)
  • Chronic myeloid leukemia is a common cancer in human, so the goal of this study was the use of natural compound such as cell wall obtained from Saccharomyces cerevisiae (S. cerevisiae) and Saccharomyces boulardi (S. boulardi) and zinc nanoparticles on the growth inhibition of K562 cell line. (ac.ir)
  • The results showed that S. boulardi cell wall significantly (p=0.029) inhibits the growth of K562 cell line compared to S. cerevisiae . (ac.ir)
  • Also zinc nanoparticles significantly (p=0.021) inhibit K562 cell line. (ac.ir)
  • Based on this finding it should be concluded that combination of zinc nanoparticles with Saccharomyces cell walls could inhibit the growth of K562 cell line in vitro . (ac.ir)
  • T2CA inhibited the expression of phosphorylated galectin-3 expression in human glioblastoma cell line which in turn inhibits the activity of MMP2 and MMP9 proteins in zymography investigations and inhibits migration of cancer cells in in vitro scratch assay. (arjournals.org)
  • 216 sets of gene knockdowns changing fitness of each cell line relative to other cell lines from the Achilles Cell Line Gene Essentiality Profiles dataset. (maayanlab.cloud)
  • Human renal cell adenocarcinoma cell line, established from a 58 years old caucasian male. (maayanlab.cloud)
  • A immortal human brain-derived cell line cell that has the characteristics: Human cell line derived from glioblastoma. (maayanlab.cloud)
  • Human skin melanoma cell line, established from a 43 years adult caucasian male. (maayanlab.cloud)
  • Human pancreatic adenocarcinoma cell line, established from a 61 year old human female. (maayanlab.cloud)
  • The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line by limiting dilution. (maayanlab.cloud)
  • A immortal human bone marrow-derived cell line cell that has the characteristics: GM-CSF- or IL-3-dependent cell line. (maayanlab.cloud)
  • Human colon colorectal carcinoma cell line from a male organism. (maayanlab.cloud)
  • A immortal human blood cell line cell that has the characteristics: AML with Ph' chromosome and t(3;3)(q21;q26). (maayanlab.cloud)
  • Human brain glioma cell line. (maayanlab.cloud)
  • Human pancreatic cancer cell line. (maayanlab.cloud)
  • A immortal human stomach-derived cell line cell that has the characteristics: Stomach cancer, but producing Nagao-type alkaline phosphatase. (maayanlab.cloud)
  • Malignant meningioma cell line. (maayanlab.cloud)
  • A immortal human ovary-derived fibroblast cell line cell that has the characteristics: Clear cell adenocarcinoma. (maayanlab.cloud)
  • Combining IR spectral data with the results of complementary biochemical investigations carried out in samples by different analytical methods we identified and cross-validated IR signatures of drugs targeting the oncogenic protein BCR/ABL and its associated abnormal tyrosine kinase activity in K562 cell line. (univr.it)
  • In the present study, we investigated the effect of the marine microalga Skeletonema marinoi (S.M.), a ubiquitous diatom that forms massive blooms in the oceans, on the human leukemia cell line K562. (unina.it)
  • As a second step, an in vitro IM-resistant model (K-562 CML cell line) was established. (unibo.it)
  • MTT assay was used to evaluate cell growth inhibition. (edu.hk)
  • The apoptosis rate was increased on SKBR-3 at 50% cell growth inhibition (IC50) as determined by Annexin-V/Propidium iodide double staining. (iyte.edu.tr)
  • on human chronic myeloid leukemia K562 cells. (edu.hk)
  • Human erythroleukemic K562 cells represent the prototypical cell culture model of chronic myeloid leukemia (CML). (bvsalud.org)
  • This is extremely useful to understand the effect of human variation both in homeostasis and diseased state such as hepatic or blood cell disorders. (biologists.com)
  • γδ T cells, specifically the Vγ9Vδ2 subset, which is the dominate subset among γδ T cells in human peripheral blood, recognize target cells in a human leukocyte antigen (HLA)-independent manner. (nature.com)
  • In this study, mechanisms of resistance to imatinib-induced apoptosis in human K562 cells were examined. (metu.edu.tr)
  • Dr. Sadelain demonstrated that human peripheral blood T cells could be effectively redirected to recognize the CO19 antigen and eradicate established, systemic human B cell tumors borne by immunodeficient mice, This was the first publication showing that genetically modified human T cells can induce durable remissions in an in vivo setting. (acgtfoundation.org)
  • The inhibitory effect of T2CA on phopho-galectin-3 expression and the activation of T-cytotoxic and NK cells suggested its protective effects against pathogenic infections and human malignancies via its immune stimulating mechanisms. (arjournals.org)
  • The hybrid virus could infect human airway cells and caused disease in mice, according to the team's results, which were published in Nature Medicine . (alecomm.com)
  • The results demonstrate the ability of the SHC014 surface protein to bind and infect human cells, validating concerns that this virus-or other coronaviruses found in bat species-may be capable of making the leap to people without first evolving in an intermediate host, Nature reported. (alecomm.com)
  • Cell lines and cell tradition The K562 (CML), KU812 (CML), MEG-01 (CML), MV4-11 (AML), MOLM14 (AML), U937 (AML), REC-1 (Human being B-cell lymphoma cell), HL-60 (Human being promyelocytic leukemia cells), MEC-1(CLL), CHL. (molecularcircuit.com)
  • ASXL1-MT mice displayed features of human-associated MDS, including multi-lineage myelodysplasia, pancytopenia, and occasional progression to overt leukemia. (jci.org)
  • Due to their HLA-independent mode of action, allogeneic Vγ9Vδ2 T cells can be considered for clinical application. (nature.com)
  • To apply allogeneic Vγ9Vδ2 T cells in adoptive immunotherapy, the methodology used to obtain adequate cell numbers with optimal effector function in vitro needs to be optimized, and clinical safety and efficacy also need to be proven. (nature.com)
  • Furthermore, a phase I clinical trial in 132 late-stage cancer patients with a total of 414 cell infusions unequivocally validated the clinical safety of allogeneic Vγ9Vδ2 T cells. (nature.com)
  • Among these 132 patients, 8 liver cancer patients and 10 lung cancer patients who received ≥5 cell infusions showed greatly prolonged survival, which preliminarily verified the efficacy of allogeneic Vγ9Vδ2 T-cell therapy. (nature.com)
  • Our clinical studies underscore the safety and efficacy of allogeneic Vγ9Vδ2 T-cell immunotherapy, which will inspire further clinical investigations and eventually benefit cancer patients. (nature.com)
  • We have recently demonstrated that vaccination with irradiated murine allogeneic ovarian cancer cells secreting heat shock protein 70 (Hsp70) were capable of generating potent tumor antigen-specific immune responses and strong anti-tumor effects against ovarian cancers (Chang et al. (acgtfoundation.org)
  • Molecular Monito-ring of BCR-ABL Transcripts after Allogeneic Stem Cell Transplantation for Chronic Myeloid Leukemia. (udea.edu.co)
  • MTT assay was used to investigate the effect of IVM on cancer cells growth in vitro . (alecomm.com)
  • En: WHO Classification of Tumours of Haema-topoietic and Lymphoid Tissues. (udea.edu.co)
  • Hematological malignancies, which include lymphoid, myeloid, histiocytic, and mast cell neoplasms, are a heterogeneous group of diseases of diverse incidence, pathogenesis and prognosis (Kornblau et al . (bbrc.in)
  • Therefore, K562 cells have been widely used for investigating the BCR/ABL1 oncogene and the tyrosine kinase inhibitor, imatinib-mesylate. (bvsalud.org)
  • Overall, K562 cell culture systems remain widely used for the investigation of novel therapeutics for CML, which is particularly important in cases of imatinib-mesylate resistance. (bvsalud.org)
  • Discovery and development of novel drugs that are capable of overcoming drug resistance in tumor cells are urgently needed clinically. (alecomm.com)
  • Recently, it has been reported by many workers that the potential functions of exosomes contributed to various aspects of hematological tumorigenesis, particularly with a focus on the exosome-mediated tumor progression, metastasis, drug resistance and immune escape by altering the function of receiver cells via diverse exosomal cargoes including proteins, DNA, messenger RNAs (mRNAs), and microRNAs) (Whiteside and Boyiadzis, (2017). (bbrc.in)
  • Chemotherapy is one of the most important treatment options in the treatment of all types of leukemia, but multi-drug resistance often precludes the success of this treatment. (ijpsonline.com)
  • However, the applicability of such traditional chemotherapeutic approaches is primarily limited due to acquired Multi-Drug Resistance (MDR) in cells, which is acquired through the production of anti-apoptotic cascades and the overexpression of cell membrane-based drug efflux pumps, such as P-glycoprotein, for self-defense. (ijpsonline.com)
  • The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
  • Our results indicated that ivermectin at its very low dose, which did not induce obvious cytotoxicity, drastically reversed the resistance of the tumor cells to the chemotherapeutic drugs both in vitro and in vivo . (alecomm.com)
  • This first one is an in vitro study of cultured micro glial cells treated with LPS. (lymecore.com)
  • Bruceine D exhibited potent cytotoxicity to K562 cells with IC50 of 6.37 ± 0.39 μM. (edu.hk)
  • In addition, the BCR-ABL fusion gene product, a constitutively activated tyrosine kinase which is crucial for the development of chronic myeloid leukemia (CML), is highly sensitive to bosutinib. (mdm2-receptor.com)
  • Methods: We evaluated the IRF4 expression kinetics during tyrosine kinase inhibitor (TKI) treatment in a cohort of 116 chronic myeloid leukemia (CML) patients to elucidate its role in the disease course. (karger.com)
  • Based on the encouraging preclinical data, we propose to generate a clinical grade tumor cell-based vaccine engineered to secrete high levels of Hsp70. (acgtfoundation.org)
  • The ability to induce these changes in K562 cell cycle and cell cycle regulation provides targets for cancer drugs. (wikipedia.org)
  • Annexin-V/PI movement cytometric experiments had been performed to determine if 17-AAG alone or in combination with cisplatin could induce A549 cell apoptosis. (hsp90-inhibitors.com)
  • cells were described to induce tumors in athymic mice and to synthesize lecithin. (maayanlab.cloud)
  • They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)
  • Immunotherapy using gene-transduced tumor cells has emerged as a potentially plausible approach for the control of advanced stage ovarian cancer. (acgtfoundation.org)
  • Western blot hybridization studies showed that T2CA downregulates the expression of total gal 3 proteins and extended the survival of AKR/J mice injected with Ehrlich ascites tumor cells in the Kepler-Meier survival curve analysis. (arjournals.org)
  • Exosomes, which are 30- to 120-nm vesicles, are released by most types of cells, including tumor cells. (bbrc.in)
  • The change in phenotype induces a decrease in the growth rate and leads the K562 cells to the terminal path of becoming mature erythroids, monocytes, and mature macrophages. (wikipedia.org)
  • K562 cells can spontaneously develop characteristics similar to early-stage erythrocytes, granulocytes and monocytes and are easily killed by natural killer cells as they lack the MHC complex required to inhibit NK activity. (wikipedia.org)
  • When the levels of these components are thrown off, they can either no longer inhibit apoptosis of the cancer cells, a role fulfilled by BCR/ABL, or they cause apoptosis to be induced, in the same vein as Bax and cytochrome C. These components are key in the mitochondria, and due to this, it has been supported that apoptosis uses the mitochondrial apoptosis pathway. (wikipedia.org)
  • Together with a description of morphology, infrared spectral analysis, and examination of metabolic properties, we provide a comprehensive characterization of K562 cells. (bvsalud.org)
  • Morphology of the cells 6 days after G-CSF stimulation was assessed by Wright-Giemsa staining (bottom). (jci.org)
  • In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
  • Further, K562 cells overexpress transferrin receptors (TfR) and have been used as a model for targeting cytotoxic therapies, via receptor-mediated endocytosis. (bvsalud.org)
  • The T2CA activated T-cytotoxic and NK cells significantly contributing to its immune stimulating effect. (arjournals.org)
  • Doxorubicin conjugated micelles with HER-2 peptide (DOX-HER-2-NCs) had more cytotoxic effects on HER-2 positive cells. (iyte.edu.tr)
  • Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
  • Immunocytochemistry/ Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. (novusbio.com)
  • We confirm the high expression of TfR on K562 cells using immunofluorescence and cell-surface receptor binding radioassays. (bvsalud.org)
  • Then, the MVB's can either fuse with lysosomes resulting in degradation of intra-luminal contents or they can secrete their content as exosomes outside the donor cells. (bbrc.in)
  • Rowley, J.D. A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and giemsa staining. (eurekaselect.com)
  • Chronic myeloid leukaemia. (udea.edu.co)
  • Pesach J & Ben-Yehuda D. Molecular evolution of chronic myeloid leukaemia. (udea.edu.co)
  • Aimed at developing accurate, reliable and cost-saving analytical techniques for drugs screening we evaluated the potential of Fourier Transform (FT) InfraRed (IR) microspectroscopy (microFTIR) as a quantitative pre-diagnostic approach for the rapid identification of IR signatures of drugs targeting specific molecular pathways causing Chronic Myeloid Leukemia (CML). (univr.it)
  • Chronic myeloid leukemia (CML) is a myeloproliferative disease that activates multiple signaling pathways, causing cells to produce higher levels of reactive oxygen species (ROS). (unina.it)
  • We have developed a genetically modified tumor cell vaccine for the treatment of myeloid malignancies. (acgtfoundation.org)
  • In particular, ASXL1 mutations are common in patients with hematologic malignancies associated with myelodysplasia, including myelodysplastic syndromes (MDSs), and chronic myelomonocytic leukemia. (jci.org)
  • Therefore, we co-cultured AML cells with macrophages. (frontiersin.org)
  • Macrophages are the most abundant white blood cells in the immune microenvironment and have a high degree of heterogeneity. (frontiersin.org)
  • Galectin three is produced by the macrophages and other myeloid tissues and response to injury and infection. (lymecore.com)
  • Here, we have characterized K562 cells focusing on the karyotype of cells in prolonged culture, regulation of expression of TfR in wildtype (WT) and doxorubicin-resistant cells, and responses to histone deacetylase inhibition (HDACi). (bvsalud.org)
  • The corresponding IR spectral profiles were assumed to represent drug-resistant and drug-sensitive cells. (univr.it)
  • Leukemia cells in patients are intrinsically resistant to standard chemotherapy, and resistance development in leukemia cells toward drugs results in treatment failure[ 4 , 5 ]. (ijpsonline.com)
  • MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
  • Similarly, single cell methods are vital in stem cell research and cancer biology, where isolated populations of primary cells are heterogeneous due to limitations in purification protocols, and it is often a minority cell population that is the most relevant. (justia.com)
  • In cancer biology, controlling leukemia remains a big challenge. (ijpsonline.com)
  • Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
  • In the CML cell K562 inoculated xenograft mouse model, dental administration of 100 mg/kg/d of CHMFL-074 accomplished a tumor development inhibition (TGI) of 65% without exhibiting obvious toxicity. (molecularcircuit.com)
  • D) American blot evaluation with antibodies particular towards the indicated protein from tumor lysates ready through the K562 xenograft mice upon the conclusion of the indicated remedies. (molecularcircuit.com)
  • Vγ9Vδ2 T cells recognize pyrophosphates secreted by many microbes or overproduced by malignant cells in the context of butyrophilin 3A1 molecules. (nature.com)
  • Chronic myeloid leukemia (CML) is a myeloproliferative disease caused due to translocation between chromosome 9 and 22 leading to a chimeric gene product known as Bcr-Abl. (eurekaselect.com)
  • It led to loss of Δψm, release of cytochrome c, activation of caspases-9, -3 and cleavage of PARP, which suggested that bruceine D induced apoptosis of K562 cells through mitochondrial pathway. (edu.hk)
  • et al, Effect of Fushengong Decoction on PTEN/PI3K/AKT/NF-KB Pathway in Rats With Chronic Renal Failure via Dual-Dimension Network Pharmacology Strategy.In Front Pharmacol on 2022 Mar 15 by Hongyu Luo, Munan Wang,et al. (sabbiotech.cn)
  • et al, Effect of Fushengong Decoction on PTEN/PI3K/AKT/NF-κB Pathway in Rats With Chronic Renal Failure via Dual-Dimension Network Pharmacology Strategy. (sabbiotech.cn)
  • The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes. (wikipedia.org)
  • There are many different cellular components involved in the cycle of apoptosis such as BCR/ABL, Bcl-2, Bax protein, and cytochrome C. The tumor suppressor gene p53 is also important in the cell cycle regulation of K562 cells. (wikipedia.org)
  • For example, single cell analysis allows for the direct measurement of gene expression kinetics, or for the unambiguous identification of co-regulated genes, even in the presence of de-synchronization and heterogeneity that could obscure population-averaged measurements. (justia.com)
  • Thus, many of the basic functionalities for microfluidic single cell gene expression analysis have been demonstrated in isolation, including cell manipulation and trapping (9, 10), RNA purification and cDNA synthesis (11-13), and microfluidic qPCR (14) following off-chip cell isolation cDNA synthesis and preamplification. (justia.com)
  • The screen was done in two cell lines: chronic myeloid leukemia (CML) K562 cells and RPE1 cells, both engineered to express dCas9-KRAB, which is a catalytically inactive version of Cas9 that doesn't cut DNA, fused with a KRAB domain which represses the gene. (centuryofbio.com)
  • Additionally, DOX-HER-2-NCs resulted in cell cycle arrest at G2/M-phase in response to IC50 value. (iyte.edu.tr)
  • Cell lysates were also subject to immunoblotting with anti-FLAG Ab or anti-Myc Ab. (jci.org)
  • B ) Expression of ASXL1-WT and its mutants in 293T cells or 32Dcl3 cells transiently transfected or stably transduced with a FLAG-ASXL1-MT1, ASXL1-MT2, ASXL1-WT, or an empty vector (pMYs-IG). (jci.org)
  • High-throughput single cell measurement technologies are therefore of interest and have broad applications in clinical and research settings. (justia.com)
  • The engineered T cells eradicate large tumors in pre-clinical experiments. (acgtfoundation.org)
  • But these anti-cancer properties would warrant further study on the clinical application of yeast cell wall. (ac.ir)
  • 2. Conduct a clinical trial in MDS testing K562/GM-CSF vaccination integrated with the systemic agent(s) identified in aim 1 as being most active in combination with GM-CSF tumor vaccines (years 2 and 3). (acgtfoundation.org)
  • Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
  • Furthermore, there are several ways by which exosomes are taken up by recipient cells: receptor- or lipiddraft-mediated endocytosis, phagocytosis, macropinocytosis, or fusion with the plasma membrane of a target cell, For transportation, exosomes mainly originate from multivesicular bodies (MVB's) in the cells which are produced by the invagination of endosomal limiting membrane (Pfrieger et al . (bbrc.in)
  • These heterozygous somatic mutations most regularly consist of a point mutation within exon 21, leading to an amino acid substitution or in-frame deletions within exon 19.Kinase domain mutations result in constitutive activation of EGFR by jak2 inhibitors selleck chemicals destabilization in the autoinhibited conformation on the receptor.In mutant EGFR tumors, cell survival is dependent on EGFR signaling, a phenomenon termed ?oncogene addiction? (hsp90-inhibitors.com)
  • Although loss-of-function ASXL1 mutations promote myeloid transformation, a large subset of ASXL1 mutations is thought to result in stable truncation of ASXL1. (jci.org)
  • Moreover, γδ T cells can directly kill target cells without the involvement of dendritic cells (DCs) and perform dual functional roles in antitumor and anti-infective immunity. (nature.com)
  • NK cell receptors in antiviral immunity. (arjournals.org)
  • These functions are necessary in cells in order to divide and regenerate tissues, and play a maintenance role in homeostatic functions. (wikipedia.org)
  • We asked regardless of whether the antiproliferative action of 17-AAG was as a consequence of cell cycle arrest, apoptosis, or each. (hsp90-inhibitors.com)
  • Unsupervised pattern recognition performed by Hierarchical Cluster Analysis (HCA) clustered the spectra of single K562 cells in two distinct groups roughly corresponding to living and to apoptotic cells, respectively. (univr.it)
  • Significant variations with increasing percentages of apoptotic cells were observed after the treatment of K562 cells with drugs that directly or indirectly target BCR/ABL. (univr.it)
  • Yamaoka K, Ohno S, Kawasaki H, Suzuki K. Overexpression of a beta-galactoside binding protein causes transformation of BALB3T3 fibroblast cells. (arjournals.org)
  • Recognition of a virus-encoded ligand by a natural killer cell activation receptor. (arjournals.org)
  • Galectin-3 facilitates cell motility in gastric cancer by up-regulating protease-activated receptor-1 (PAR-1) and matrix metalloproteinase-1 (MMP-1). (arjournals.org)
  • Galectin three promotes immune suppression in chronic inflammatory conditions by forming these cross-links it suppresses immune surveillance It blocks the T cell receptor. (lymecore.com)
  • The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
  • In such cases, stem cell transplant therapy, which is limited by donor availability, is the only proven cure for the patients. (eurekaselect.com)
  • The introduction of genes that code for tumor-specific receptors into T lymphocytes, which are cells of the immune system that mediate tumor elimination, was a novel approach to cancer therapy. (acgtfoundation.org)
  • His interests currently include nutrition and dietary therapy, herbal treatments, bio-identical HRT and intravenous nutritional therapy for chronic diseases such as CFS, adrenal fatigue, thyroid disorders, male and female menopause and cancer. (thecancerrevolution.co.uk)
  • Introduction - Although imatinib (IM) is a recognized gold standard in chronic myeloid leukemia (CML) therapy, resistance has emerged in a significant proportion of patients. (unibo.it)