• Abstract: '[Researchers] have determined the nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map. (harvard.edu)
  • P. 2194 middle column 2nd paragraph: 'Genome assembly relied on the use of several types of data, including clone-based sequence, whole-genome sequence from libraries with three insert sizes [2 kb, 10 kb, and 130 kb], and a Bacterial Artificial Chromosome (BAC)-based sequence-tagged site (STS) content map. (harvard.edu)
  • We constructed Drosophila melanogaster bacterial artificial chromosome libraries with 21-kilobase and 83-kilobase inserts in the P[acman] system. (nih.gov)
  • We isolated a bacterial artificial chromosome clone composed of all 17 exons and 16 introns from the individual gene (Johnston et al. (cancer-ecosystem.com)
  • A) Schematic of bacterial artificial chromosome clone formulated with all 17 exons and 16 introns from the individual gene plus huge 5 and 3 flanking sequences. (cancer-ecosystem.com)
  • Methods We selected bacterial artificial chromosome (BAC) clones harboring key IGC genes from public and private libraries and sequenced the inserts with long-read sequencing technologies. (usda.gov)
  • An important breakthrough in horse Y chromosome research came with the availability of new genomics tools and resources, such as large-insert genomic bacterial artificial chromosome (BAC) libraries (for review, see Rubes et al. (veteriankey.com)
  • First, Tim Graubert and colleagues describe the whole-genome sequencing of an MDS-derived secondary AML tumor and a matched normal (skin) sample. (massgenomics.org)
  • To investigate the possible expansion of a single clade , we performed whole-genome sequencing for 43 Japan and 10 global ST131 isolates with bla CTX-M-27 (n = 16), bla CTX-M-14 (n = 16), bla CTX-M-15 (n = 13), and others (n = 8). (cdc.gov)
  • Recent studies using whole-genome sequencing (WGS) analysis revealed that ST131 comprises different lineages or clades ( 4 , 5 ). (cdc.gov)
  • By applying whole genome and quantitative chromatin conformation analysis {chromatin interaction analysis with paired end tag sequencing (ChIA-PET), and real time chromosome conformation capture (3C) assay}, we observed that the MEF2C-bound enhancer and transcription start site (TSS) of KLF4 came into closer spatial proximity by pitavastatin treatment. (soken.ac.jp)
  • High-resolution comparative mapping between human chromosomes 4 and 8 and bovine chromosome 27 provides genes and segments serving as positional candidates for udder health in cattle. (fbn-dummerstorf.de)
  • This genome survey reveals numerous genes, genome-wide repeats, simple sequence repeats (potential genetic markers), and CpG islands (potential gene initiation sites). (jcvi.org)
  • The heterochromatin consists mainly of short, simple sequence elements repeated for many megabases, occasionally interrupted by inserted transposable elements, and tandem arrays of ribosomal RNA genes. (harvard.edu)
  • The materials themselves are highly accurate readouts of specific genes that can act as an answer key for checking the results of a particular sequencing method. (labmanager.com)
  • The newest addition could be useful in identifying and diagnosing the few known conditions related to genes in the Y chromosome. (labmanager.com)
  • There are certainly aspects of fertility and some genetic disorders that are connected to genes in the Y chromosome," Zook said. (labmanager.com)
  • Genes of interest are identified using next-gen high-throughput sequencing and barcode multiplexing is used to reduce experimental cost. (biomedcentral.com)
  • Analysis of co-occurring transposons integration sites in single cell clone allows for the identification of genes that might act cooperatively to produce drug resistance a level of information not accessible using RNAi or ORF expression screening approaches. (biomedcentral.com)
  • This showed chromosomes carry genes and described the chromosome recombination. (timetoast.com)
  • Barbara McClintock found out that genes can jump around on chromosomes. (timetoast.com)
  • When Barbara figured out that genes can "jump" she than correlated microscopic arrangments of chromosome placements. (timetoast.com)
  • A researcher may access the web page of these sites and perform a search according to genetic tag, sequence information, location on chromosome, related genetic disorders and diseases, as well as the names of genes themselves. (enculturation.net)
  • The introduction of mutant loxP websites within the integrating plasmids permits repeated elimination of antibiotic resistance genes and different plasmid sequences. (bioconstructor.com)
  • With this feature, Casilio can now conduct different functions at different genomic sites--e.g., upregulating one set of genes while repressing another set. (jax.org)
  • Characterisation of breakpoints in patients with apparently balanced constitutional chromosome rearrangements and phenotypic abnormalities has proved an invaluable strategy for identifying disease causing genes, especially those on the X chromosome. (bmj.com)
  • 1. Create an "array design" with all genes of interest (ideally this would be the largest set possible, e.g. known genes + predicted genes of all qualities, perhaps even predicted genes from the new sequence data). (lu.se)
  • Isolation and characterization of bovine chromosome region specific sequence-tagged microsatellite sites (STMS). (fbn-dummerstorf.de)
  • Targeted generation of 16 sequence-tagged sites for bovine chromosome region 5q21-q25 by microdissection. (fbn-dummerstorf.de)
  • Single pass sequencing of cDNA clones provides a rapid route to gene discovery and production of EST's (2). (le.ac.uk)
  • Systematic sequencing of cDNA clones derived from xylem and leaf tissue will produce a new generation of research tools for conifer biology. (le.ac.uk)
  • 1111, Yata, Mishima, Shizuoka 411-8540, Japan REFERENCE 2 AUTHORS Mishima,H., Shizuoka,T. and Fuji,I. TITLE Glyceraldehyde-3-phosphate dehydrogenase expressed in human liver JOURNAL Unpublished (2002) COMMENT Human cDNA sequencing project. (nig.ac.jp)
  • 450 /chromosome="12" /clone="GT200015" /clone_lib="lambda gt11 human liver cDNA (GeneTech. (nig.ac.jp)
  • Deep cDNA sequencing demonstrated that both wild-type and mutant alleles were expressed . (massgenomics.org)
  • The high-throughput - next generation sequencing (HT-NGS) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the Sanger method. (springer.com)
  • With the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cost of $1,000 each is seemed to be realistically feasible in the near future. (springer.com)
  • The completion of the first human genome drafts (Yamey 2000 ) was just a start of the modern DNA sequencing era which resulted in further invention, improved development toward new advanced strategies of high-throughput DNA sequencing, so called the " high-throughput next generation sequencing " (HT-NGS). (springer.com)
  • Drug resistance can be mediated by further genetic and/or epigenetic changes in the tumor and, with the advent of high throughput sequencing, it is now feasible to systematically survey mutations in tumor genomes from patients following resistance development. (biomedcentral.com)
  • gambiae karyotyped either cytogenetically or molecularly using a genotyping-in-thousands by sequencing (GT-seq) high-throughput approach that employs targeted sequencing of multiplexed PCR amplicons. (cdc.gov)
  • I'm just thinking out loud about how to incorporate high throughput transcriptome sequencing data into BASE. (lu.se)
  • 7- 9 All reported mutations are single nucleotide alterations leading to splice site, missense, nonsense, or frameshift mutations, or intra-exonic deletions and insertions of up to four nucleotides, which have been detected through exonic PCR amplifications and sequencing. (bmj.com)
  • In contrast to the abundance mutations in SDHB and SDHD , only a single multiply affected family and an isolated case, containing a single nucleotide initiation codon and a splice site mutations in SDHC , respectively, have been described by Niemann et al . (bmj.com)
  • These clones can be integrated into predetermined attP sites in the genome using UC31 integrase to rescue mutations. (nih.gov)
  • The authors undertook systematic sequencing of U2AF1 exons in 150 MDS cases, and found that 8.7% had mutations at Ser34. (massgenomics.org)
  • OBJECTIVE: Mutations in the FUS gene on chromosome 16 have been recently discovered as a cause of familial amyotrophic lateral sclerosis (FALS). (bvsalud.org)
  • We report here 13 mutations in the fused in sarcoma/translated in liposarcoma (FUS/TLS) gene on chromosome 16 that were specific for familial ALS. (bvsalud.org)
  • METHODS: To establish the prevalence of VAPB mutations, we screened 80 familial ALS samples by DNA sequencing. (bvsalud.org)
  • Unrepaired or incorrectly repaired DNA damage may lead to loss of heterozygosity, mutations, deletions, genomic rearrangements and chromosome loss. (cea.fr)
  • A previous population genomic analysis showed that protein sequences are under greater functional constraints in thermophiles than in mesophiles, suggesting that mutations are functionally more deleterious in thermophiles than in mesophiles [ 4 ]. (nature.com)
  • Congenital chromosome changes and other mutations in the DNA sequence can cause many conditions, for example cancer. (lu.se)
  • Generation of chromosome fragment specific DNA sequences by microdissection and DOP-PCR. (fbn-dummerstorf.de)
  • 2009), chromosome flow sorting and microdissection technology, and various genomic DNA amplification systems (Telenius et al. (veteriankey.com)
  • Sites in the DNA sequence where individuals differ at a single DNA base are called single nucleotide polymorphisms (SNPs). (genome.gov)
  • These primers are the point of origin for nucleotide binding, and the specific primer sequence will determine what region of DNA is replicated. (azolifesciences.com)
  • In order to identify single nucleotide polymorphism (SNP) markers that would be suitable for assays, we aligned sequence data from 125 Holstein bulls to the alternative haplotypes. (usda.gov)
  • The Pumilio RNA-binding protein system allows the design of a simple nucleotide code that recognizes an eight-nucleotide RNA sequence, called a PUF domain. (jax.org)
  • The sequence of the four nucleotide bases encodes DNA's information. (genome.gov)
  • We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
  • METHODS: We designed tetra-primer amplification refractory mutation system (ARMS)-PCR assays based on five tag single-nucleotide polymorphisms (SNPs) previously shown to be strongly correlated with 2Ru inversion orientation. (cdc.gov)
  • The STC resource, containing end sequences, fingerprints, and arrayed BACs, creates a map where the interrelationships of the individual BAC clones are resolved through their STCs as overlapping BAC clones are sequenced. (jcvi.org)
  • Once a seed or initiation BAC clone is sequenced, the minimum overlapping 5' and 3' BAC clones can be identified computationally and sequenced. (jcvi.org)
  • By reiterating this "sequence-then-map by computer analysis against the STC database" strategy, a minimum tiling path of clones can be sequenced at a rate that is primarily limited by the sequencing throughput of individual genome centers. (jcvi.org)
  • It also illustrates the power of the STC strategy for creating minimum tiling paths of BAC clones for large-scale genomic sequencing. (jcvi.org)
  • The majority of the marker sequences were derived from randomly chosen clones from four different chromosome 6-enriched plasmid libraries or from subclones of YACs previously mapped to chromosome 6. (le.ac.uk)
  • In addition, we find that a high proportion of the plasmid sequences derived from gel-enriched chromosome 6 (and that form the basis of a chromosome-specific sequencing project) originates from other chromosomes. (le.ac.uk)
  • Besides the interactions that position eS25 at its location in the E site region of the 40S ribosomal subunit, eS25 has been suggested to interact with other cellular proteins. (wikipedia.org)
  • Comparative mapping of sheep inhibin subunit βB to chromosome 2 in sheep and cattle by fluorescence in situ hybridization. (fbn-dummerstorf.de)
  • The human ATP synthase beta subunit gene: sequence analysis, chromosome assignment, and differential expression. (joplink.net)
  • The flavin together with the iron-sulfur clusters function to mediate electron transfer from the dicarboxylate binding site on the Fp subunit to the membrane-anchor domain where quinone is reduced to quinol. (lu.se)
  • In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)
  • sequencing-uml-draft-1.png ​ ( 74.9 KB ) - added by Nicklas Nordborg 13 years ago . (lu.se)
  • sequencing-biomaterials-draft-2.png ​ ( 97.7 KB ) - added by Nicklas Nordborg 13 years ago . (lu.se)
  • An examination of the content and distribution of repetitive DNA sequences to identify the location of such sequences within specific chromosomes and their conservation across species of the Pinaceae family. (le.ac.uk)
  • Fluorescent in situ hybridisation (FISH) on metaphase chromosomes and on extended chromatin will be used to physically locate repetitive as well as single copy DNA sequences on conifer chromosomes and to integrate physical and genetic maps. (le.ac.uk)
  • Chromosomes all contain sections of very repetitive DNA, but well over half of the Y chromosome is like that," said study co-author Justin Zook, who leads NIST's Genome in a Bottle (GIAB) consortium. (labmanager.com)
  • 1992). Due to the largely heterochromatic nature of ECAY and repeat-biased amplification of DOP-PCR, microdissected probes tend to be enriched with repetitive sequences, while euchromatic sequences remain underrepresented. (veteriankey.com)
  • Telomeres are structures at the ends of chromosomes that contain repetitive stretches of DNA. (cdc.gov)
  • The sequence-tagged connector (STC) strategy proposes to generate sequence tags densely scattered (every 3.3 kilobases) across the human genome by arraying 450,000 bacterial artificial chromosomes (BACs) with randomly cleaved inserts, sequencing both ends of each, and preparing a restriction enzyme fingerprint of each. (jcvi.org)
  • DSBs are first detected and signaled by the DNA damage checkpoint that triggers cell cycle arrest, providing time for the cell to repair damaged chromosomes before entering mitosis. (cea.fr)
  • Walter Flemming was one of the first cytologists to fully detail the movement of chromosomes during mitosis, as well as cell division. (timetoast.com)
  • Results: With due attention to the data resulted from multiplex polymerase chain reaction and agarose gel electrophoresis in order to recognize Y chromosome micro deletions in azoospermia factor region, in this work, all the bands related to the mentioned primers which were formed during the polymerase chain reaction, were detected on the gel obviously. (virascience.com)
  • It means that none of the samples neither the fifty fertile men nor the ninety-two patient men had complete micro deletions in their Y chromosome. (virascience.com)
  • Conclusion: This study suggests that there is no correlation between Y chromosome micro deletions and occurrence of recurrent pregnancy loss in Iranian population. (virascience.com)
  • The only true Y chromosome structural rearrangements, reported so far, are large-scale ECAY deletions in 64,XY sex reversal mares (Raudsepp et al. (veteriankey.com)
  • The probes have been used for molecular hybridizations (fluorescence in situ hybridization, or FISH) in clinical cytogenetics, Y chromosome mapping, sequencing, gene discovery, and comparative Y chromosome studies between the horse and other equids/ Perssodactyls. (veteriankey.com)
  • In other circumstances, the testing method was neither germline or somatic, but used other means for identifying genetic risk (e.g., family history), or diagnosis, including biomarkers (objective measures of a biological state or condition within cells or organisms) and cytogenetics (the study of chromosomes and their inheritance). (cdc.gov)
  • Swine-specific sequence tagged (Microsatellite) sites, STS and STMS, were assigned chromosomally by polymerase chain reaction analysis of the somatic cell hybrid panel. (usda.gov)
  • Segregating populations will be characterised with both single locus (microsatellites) and multi-locus (AFLPs) marker systems providing framework maps for the eventual location of cDNAs generated from single pass sequencing. (le.ac.uk)
  • Positions Contents --------- -------- 01-05 'LOCUS' 06-12 spaces 13-28 Locus name 29-29 space 30-40 Length of sequence, right-justified 41-41 space 42-43 'bp' 44-47 spaces 48-54 DNA, RNA, mRNA, rRNA, tRNA or cRNA, left justified 55-55 space 56-63 'linear' followed by two spaces, or 'circular' 64-64 space 65-67 The division code (see '3.1. (nig.ac.jp)
  • The MAT locus was tagged by insertion of 256 lac operators at 4.4 kb of the HO cut site. (cea.fr)
  • Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)
  • During this period, tremendous success has been achieved in the fields of decoding of human genome, technological advancement of new era of human genome applications, toward personalized genomes and discovery of rare variants, leveraging genome sequencing to impact on cancer researches and mammalian evolution and population structure. (springer.com)
  • Because the STC resource permits the easy integration of genetic, physical, gene, and sequence maps for chromosomes, it will be a powerful tool for the initial analysis of the human genome and other complex genomes. (jcvi.org)
  • Using them both as guides for sequencing a diverse group of over 1,200 separate genomes, they found that T2T-Y drastically improved the outcomes. (labmanager.com)
  • Arrows tag places of three pairs of PCR primers utilized to judge incorporation from the gene into DNA of transgenic mice. (cancer-ecosystem.com)
  • Incorporating the correct annealing temperature critical for the primers to bind to the correct target sequence. (azolifesciences.com)
  • They examined the sample with a combination of cutting-edge technologies-namely high fidelity and nanopore sequencing-that make the DNA fragment puzzle pieces larger and thus easier to assemble. (labmanager.com)
  • This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
  • This polymorphism is primarily attributed to the various amount of Y heterochromatin, though earlier studies also suggest variation in the euchromatic portion of the chromosome (Power, 1988). (veteriankey.com)
  • The system makes use of site-specific recombination reactions carried out by bacteriophage integrases to combine plasmids at as much as eight phage attachment websites in E. coli MG1655. (bioconstructor.com)
  • Isolation and application of chromosome 6 specific microsatellite markers for detection of QTL for milk-production traits. (fbn-dummerstorf.de)
  • FBN3 (D6S24): a bovine microsatellite derived from cosmid cIOBT475 at chromosome 6q12-14. (fbn-dummerstorf.de)
  • For the future direction, we need the in-depth genome sequence information and analysis for most of the mammals, including human to fully understand genome variation of economic traits, genetic susceptibility to diseases, and pharmacogenomics of drug response. (springer.com)
  • Year 2011 is celebrated as the 10th anniversary since the human genome was first sequenced ( www.nature.com/natureconferences/hg10years/index.html ). (springer.com)
  • Sequence-tagged connectors: a sequence approach to mapping and scanning the human genome. (jcvi.org)
  • What was once the final frontier of the human genome-the Y chromosome-has just been mapped out in its entirety. (labmanager.com)
  • Led by the National Human Genome Research Institute (NHGRI), a team of researchers at the National Institute of Standards and Technology (NIST) and many other organizations used advanced sequencing technologies to read out the full DNA sequence of the Y chromosome-a region of the genome that typically drives male reproductive development. (labmanager.com)
  • Contain a diploid set of chromosomes and make up the majority of human cells. (proprofs.com)
  • Joe Hin Tjio defined 46 as the exact number of human chromosomes. (timetoast.com)
  • Outcomes Era of transgenic mice that Aceneuramic acid hydrate exhibit the individual gene We reasoned a transgene formulated with the entire individual gene plus lengthy flanking sequences might confer tissue-specific, basal, and inducible appearance of individual P-selectin in mice as the indigenous gene will in human beings. (cancer-ecosystem.com)
  • Nucleoli, prominent nuclear subdomains and sites of ribosome biogenesis, form around nucleolar organizer regions (NORs) that contain rDNA arrays located on human acrocentric chromosome p-arms. (diagenode.com)
  • Additionally, there are two reports about infertile stallions having XXY sex chromosomes - an equine counterpart to human Kleinfelter syndrome (Kubien et al. (veteriankey.com)
  • 18- 21 Xq28 is a highly gene rich region of the human X chromosome and we focused on the X chromosome breakpoint as the identification of a disrupted gene here would be a rapid way to identify a further candidate gene for X linked mental retardation within Xq28. (bmj.com)
  • AN - check the tag INFANT HN - 2008 FX - Child Nutrition FX - Infant Nutrition Physiology FX - Milk FX - Milk, Human DH - Adolescent Nutrition DI - 052508 MN - SP6.021.067 MS - Nutrition of persons 10 through 19 years of age. (bvsalud.org)
  • We focused on MEF2C among the MEF2 family members and identified a novel functional MEF2C binding site 148 kb upstream of the KLF4 gene by chromatin immunoprecipitation along with deep sequencing (ChIP-seq) followed by luciferase assay. (soken.ac.jp)
  • Targeted construction of a high-resolution, integrated, comprehensive, and comparative map for a region specific to bovine chromosome 6 based on radiation hybrid mapping. (fbn-dummerstorf.de)
  • The HapMap is a map of these haplotype blocks and the specific SNPs that identify the haplotypes are called tag SNPs. (genome.gov)
  • One feature of nuclear organization is the existence of subcompartments in which specific DNA sequences and proteins associate creating microenvironments that can be more or less favorable for specific processes. (cea.fr)
  • Scalograms that relate to the organism Caenorhabditis elegans (C. elegans) exhibit a multitude of periodic organization of specific DNA sequences. (springeropen.com)
  • CRISPR-Cas9 cuts DNA at a precise location, guided there by a sequence-specific RNA. (jax.org)
  • dCas9 has been modified so that it no longer cuts DNA, but it can still be guided to specific sequences and will bind to them. (jax.org)
  • Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
  • 2007). This allowed isolation of ECAY sequences and generation of Y-specific molecular probes. (veteriankey.com)
  • Therefore, microdissected Y-chromosome probes are good for FISH but not suitable for cloning and sequencing of the male-specific or pseudoautosomal regions of the chromosome. (veteriankey.com)
  • We have now sequenced Y. pestis glp D gene in such remains, showing a 93-bp deletion specific for biotype Orientalis. (cdc.gov)
  • check also tag CHILD or specific HN - 2008 FX - Adolescent Nutrition FX - Infant Nutrition DH - Maternal Nutrition DI - 052505 MN - SP6.021.072 MS - Nutrition of a mother which affects the health of the INFANT as well as herself. (bvsalud.org)
  • Different proteins were known to have unique sequences, whereas it was thought that all DNA molecules have the same sequence. (proprofs.com)
  • dCas9 can be fused to protein tags or the effector domains of transcription factors (proteins that bind DNA in promoter regions and simultaneously bind other regulatory proteins via effector domains, which can activate or repress transcription and thereby affect gene expression) to develop customized DNA binding proteins. (jax.org)
  • Assignment of the fibroblast growth factor 10 (FGF10) gene to bovine chromosome 20q16→q17 by fluorescence in situ hybridization and somatic cell panel analysis. (fbn-dummerstorf.de)
  • To investigate the possibility that a novel candidate gene for XLMR was disrupted at the X chromosome translocation breakpoint, we mapped the breakpoint using fluorescence in situ hybridisation (FISH). (bmj.com)
  • The tagged DNA binds to its complementary DNA segment and can be detected by measuring the amount and type of fluorescence. (msdmanuals.com)
  • Intriguingly, we found that the X chromosome breakpoint in the daughter could not be defined by a single breakpoint spanning genomic clone and further analysis showed a 650 kb submicroscopic duplication between DXS7067 and DXS7060 on either side of the X chromosome translocation breakpoint. (bmj.com)
  • The map appears to span the entire chromosome, although marker density is greater in some regions than in others and is lowest within the telomeric region. (le.ac.uk)
  • Oryza sativa Japonica Group strain A05 chromosome 6 marker STS123 genomic sequence, sequence tagged site 480 bp linear DNA Accession: EF010228.1 GI: 169880634 PubMed Taxonomy GenBank FASTA Graphics PopSet. (centralparkcarriagesofficial.org)
  • This might be because rearrangements in the gene-poor Y chromosome have milder effect on the phenotype, as a result of which the affected individuals escape attention and are not subjected to cytogenetic analysis. (veteriankey.com)
  • 3- 6 Phenotypic abnormalities seen in cases with apparently balanced chromosome rearrangements have usually been explained by the disruption of a gene at the breakpoint causing the loss of gene function. (bmj.com)
  • A gene-based high-resolution comparative radiation hybrid map as a framework for genome sequence assembly of a bovine chromosome 6 region associated with QTL for growth, body composition, and milk performance traits. (fbn-dummerstorf.de)
  • A high resolution comparative map for a bovine chromosome 6 (BTA6) region containing QTL for production, health, and conformation traits. (fbn-dummerstorf.de)
  • The combination of these resources resulted in a set of ordered contigs spanning nearly all of the euchromatic region on each chromosome arm. (harvard.edu)
  • Y chromosome microdeletions are a class of most likely genetic factors that occur in a special zone of Y chromosome which is named azoospermia factor region. (virascience.com)
  • Within the broad susceptibility region for bipolar disorder on the pericentromeric portion of chromosome 18, the highest allele sharing in our 22-pedigree series has been found in markers mapping to 18p11.2. (rhea-db.org)
  • Many diseases involve variations in gene expression rather than genetic sequence. (jax.org)
  • Comparative mapping of sheep inhibin subunits alpha (INHA) and beta B(INHBB) to chromosome 2 in goat by FISH. (fbn-dummerstorf.de)
  • Comparative mapping of the gene coding for Inhibin-α (INHA) to Chromosome 2 in sheep and cattle. (fbn-dummerstorf.de)
  • To be able to apply such techniques, it is imperative to convert DNA characters into numerical sequences. (springeropen.com)
  • The Escherichia coli sequence type (ST) 131 C2/ H 30Rx clade with the bla CTX-M-15 gene had been most responsible for the global dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli . (cdc.gov)
  • The increase in resistance among ExPEC has resulted mainly from the recent expansion of a pandemic clonal group known as E. coli sequence type (ST) 131, which is usually multidrug resistant and is associated with CTX-M-15, the most prevalent β-lactamase among ESBL-producing ExPEC ( 2 ). (cdc.gov)
  • A comprehensive survey for polymorphisms in the bovine IFN-gamma gene reveals a highly polymorphic intronic DNA sequence allowing improved genotyping of Bovinae. (fbn-dummerstorf.de)
  • Our map largely supports previous gene-based maps of this chromosome but reveals a number of errors in the physical map. (le.ac.uk)
  • Pille Hallast's recent work, published in Nature, reveals the complexities of the male sex chromosome. (jax.org)
  • Using just the tag SNPs, researchers are able to find chromosome regions that have different haplotype distributions in the two groups of people, those with a disease or response and those without. (genome.gov)
  • So, researchers and clinicians rely on special software to piece together fragments of sequenced code in the correct order like a puzzle. (labmanager.com)
  • The researchers pitted their complete Y chromosome sequence, named T2T-Y, against the most widely used reference genome's Y chromosome parts, which are riddled with stretches of absent code. (labmanager.com)
  • A new gene regulation and labeling platform dubbed "Casilio" can simultaneously execute distinct functions at multiple areas of the genome at the same time, expanding researchers' ability to study gene function and chromosome structure, Jackson Laboratory (JAX) researchers report. (jax.org)
  • The widely used CRISPR-Cas9 gene-editing technology has enabled researchers to alter genome sequence or gene expression with great precision, says JAX Assistant Professor Albert Cheng, Ph.D., first and co-corresponding author of a paper published in Cell Research . (jax.org)
  • For example, it can be fused to domains that activate or repress gene expression, allowing researchers to increase or decrease gene expression at will without changing the actual sequence. (jax.org)
  • We further sequenced multiple and diverse isolates of this species and calculated that T. eurythermalis has a lower effective population size than other free-living prokaryotes by 1-2 orders of magnitude. (nature.com)
  • The 2Ru inversion, spanning roughly 8% of chromosome 2R, is commonly polymorphic in West African populations of An. (cdc.gov)
  • DNA isolation, genotyping of simple tandem repeat polymorphisms, PCR amplification and sequencing were performed using standard techniques 2 and all simple tandem repeat polymorphisms were amplified in the presence of 10% glycerol and 5% DMSO after labelling one oligonucleotide primer with 32 P and analysed on a 6% polyacrylamide gel. (bmj.com)
  • Mapping of the Interferon γ gene (IFNG) to Chromosomes 3 in sheep and 5 in goat by FISH. (fbn-dummerstorf.de)
  • Mapping of the gene encoding for Inhibin-βA (INHBA) to Chromosome 4q26 in sheep. (fbn-dummerstorf.de)
  • Chromosomal mapping of the bovine aromatase gene (CYP19) and an aromatase pseudogene to Chromosome 10 and syntenic group U5. (fbn-dummerstorf.de)
  • Characterisation and mapping of expressed sequence tags or EST's to serve as anchored sequences tagged sites (STS). (le.ac.uk)
  • On the surface the task of bioinformatics seems fairly straightforward: to efficiently organize the overwhelming amount of biological and genetic data produced by such projects as those involved in genomic mapping and sequencing. (enculturation.net)
  • That data served as a jumping-off point for the new study's authors, who focused their analysis on the best understood GIAB Y chromosome. (labmanager.com)
  • A machine-learning analysis tool and gamut of other advanced programs helped the team identify and assemble the pieces of the chromosome. (labmanager.com)
  • An allele is one of two or more versions of DNA sequence (a single base or a segment of bases) at a given genomic location. (genome.gov)
  • RESULTS: A successful assay was designed based on the tag SNP at position 2R, 31710303, which is highly predictive of the 2Ru genotype. (cdc.gov)
  • The results of a study published in Nature demonstrate that this advance improves DNA sequencing accuracy for the chromosome, which could help identify certain genetic disorders and potentially uncover the genetic roots of others. (labmanager.com)
  • Targeted introduction of DSBs into rDNA, but not abutting sequences, results in ATM-dependent inhibition of their transcription by RNA polymerase I. This is coupled with movement of rDNA from the nucleolar interior to anchoring points at the periphery. (diagenode.com)
  • Thus, revealing the structural and organizational features in DNA sequences is a very interesting topic. (springeropen.com)
  • Outside BASE, align the new sequences to genome or transcript sequences and calculate "intensities" for each gene on the "array design" and dump into a tab delimited raw data file. (lu.se)