The reaction is confined to organisms producing beta-lactamases, but it is sufficiently sensitive to indicate the presence of this enzyme is small amounts in strains previously considered not to produce it. (nih.gov)
This enzyme hydrolyzes the chromogenic substrate: TMB, to form a colored compound whose reaction will be stopped by sulfuric acid. (cryopep.com)
Chromogenic activity is a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. (thermalindo.com)
The specific differential activity of this agar is obtained with a proprietary lecithin substrate for the detection of the phospholipase enzyme present in the L. monocytogenes colonies growing on this media. (thermalindo.com)
Combining both the chromogenic and phospholipase enzyme reactions makes it possible to differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (thermalindo.com)
Upon cleavage of the substrate by the active enzyme (factor Xa), a colored compound is released. (medscape.com)
However PON1 is considered as a promiscuous enzyme having also the ability to hydrolyze many other substrates such as other organophosphorous compounds, non phosphorous arylesters and also lactones, which have been considered as its primary substrates [ 19 ]. (biomedcentral.com)
Dyes5
Formazan dyes are artificial chromogenic products obtained by reduction of tetrazolium salts by dehydrogenases and reductases. (wikipedia.org)
Formazans are intensely colorful compounds characterized by the following structure: [-N=N-C(R)=N-NH-], and are closely related to azo (−N=N−) dyes. (wikipedia.org)
A protocol for establishing the suitability of chromogenic dyes in the analysis of filamentous fungi with phenotype microarrays was described. (edu.pl)
Cyanine dyes that are highly charged, such as those including multiple sulfonate, alkylsulfonate, carboxylate and/or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention. (justia.com)
The present invention relates generally to the synthesis of fluorescent compounds that are analogues of cyanine dyes. (justia.com)
Assay5
The endpoint of the assay is an easy to interpret colour reaction. (biotoxicity.com)
The most commonly used methodology is the chromogenic assay, which uses a chromophore-linked substrate of factor Xa. (medscape.com)
New chromogenic substrates for the assay of alpha-amylase and (1→4)-β-D-glucanase. (megazyme.com)
RESULTS: A combined kinetic assay was developed in conditions to optimally measure FAP as well as PREP activity with Z-Gly-Pro-AMC as substrate. (inra.fr)
Using the interaction of recombinant hCD40 ligand with HEK-Blue sensor cells expressing the CD40 receptor as example, we show that such an assay can be developed based on BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium chloride) as substrate. (envite.org)
Biochemical1
If growth occurs - perform proper identification methods: Yeast identification methods Manual and automated biochemical reactions capable of identifying most but not all yeast species. (slideshare.net)
Assays4
Assays based on the use of these substrates are sensitive and highly specific for either alpha-amylase or (1→4)-β-D-glucanase. (megazyme.com)
And (b) the technical aspects of PON1 assays, in which the reagents and reaction conditions, sources of variation, quality control systems, equipment and interferences with other esterases will be discussed. (biomedcentral.com)
The spectrophotometric assays based on the ability of PON1 to hydrolyse substrates are currently more widely used, probably due to their low cost and availability. (biomedcentral.com)
SEAP activity measurement in reporter cell-based assays using BCIP / NBT as substrate. (envite.org)
Aromatic Compounds2
Friedel, R. A. and M. Orchin (1951) Ultraviolet Spectra of Aromatic Compounds. (photochemcad.com)
Helical inclusion complexes of amylose with aromatic compounds: crystallographic evidence for new V-type allomorphs. (toulouse-biotechnology-institute.fr)
Reagents2
To ensure the desired reaction is reached, TCI offers a variety of reagents and enzymes for your research. (tcichemicals.com)
Click for a list of TCI reagents according to their basic structure such as 3,4,5-Membered Cyclic Compounds, 6-Membered Heterocyclic Compounds, 6-Membered Carbocyclic Compounds, Compounds by Functional Group Classes, Compounds by Element Classes, and more. (tcichemicals.com)
Organic compounds4
belongs to the class of organic compounds known as monosaccharide phosphates. (ecmdb.ca)
Hirayama, K. (1967) Handbook of Ultraviolet and Visible Absorption Spectra of Organic Compounds. (photochemcad.com)
CRC Atlas of Spectral Data and Physical Constants for Organic Compounds. (photochemcad.com)
Peroxidase2
TMBZ is a chromogenic reagent utilized for peroxidase detection. (dojindo.com)
The subject ANS analogs are characterized in that they react with MBTH or analogs thereof in the presence of an oxidizing agent, a peroxidase and oxygen to produce a chromogenic, specifically a blue, reaction product that exhibits reduced drift. (allindianpatents.com)
Detection4
The CFX96 Touch Deep Well Real-Time PCR detection system offers precise quantification and target discrimination for up to five targets in large-volume reactions. (lifelinediag.com)
All immunostaining techniques, including IHC, utilize this important property of the antigen - antibody reaction specificity to ensure the detection of a single molecule type from a milieu of thousands of different ones (Figure 1). (technologynetworks.com)
Diagrammatic representation of the specificity of the antigen - antibody reaction that enables detection and localization of a single target in a milieu of thousands of intracellular molecules. (technologynetworks.com)
Immunostaining is an umbrella term that encompasses all the techniques that are used for the detection of molecules employing the antigen - antibody reaction. (technologynetworks.com)
Bacillus1
After having prepared a mother suspension of the sample to be analysed according to a ratio of 10 (1 part solute + 9 parts diluent for a total of 10 parts), we put 1% of it into a medium containing the gelling agent agar-agar, a chromogenic substrate which colourises the Bacillus cereus bacteria, and antibiotics which inhibits the growth of other bacteria. (lns.lu)
Specificity1
FAP shares substrate specificity with prolyl oligopeptidase (PREP), studied in (neuro)inflammation and neurodegeneration as well as cancer. (inra.fr)
Molecule1
Glucose 1-phosphate is the direct product of the reaction in which glycogen phosphorylase cleaves off a molecule of glucose from a greater glycogen structure. (ecmdb.ca)
Vitro2
Another method based on molecular biology is the polymerase chain reaction (PCR), a technique used to make many copies of a specific DNA region in vitro (in a tube rather than in an organism). (lns.lu)
We identified urolithin A (UA) as a first-in-class natural compound that induces mitophagy both in vitro and in vivo following oral consumption. (lsd1receptor.com)
Bacterial4
EBPI offers several bacterial-based tests that assess samples for cytotoxicity from acutely toxic compounds. (biotoxicity.com)
The functional similarity between human and bacterial DPP4 was confirmed using 124 potential peptide substrates. (inra.fr)
Substrate-Assisted Mechanism for the Degradation of N-glycans by a Gut Bacterial Mannoside Phosphorylase. (toulouse-biotechnology-institute.fr)
Fasting can be induced if a compound affects bacterial growth, thereby reducing the amount of food available or modifying key nutrient amounts14, or acts as repellent for the worms and prevents food intake. (lsd1receptor.com)
Catalyzes1
[10] The active site of β-galactosidase catalyzes the hydrolysis of its disaccharide substrate via "shallow" (nonproductive site) and "deep" (productive site) binding. (wikidoc.org)
Fluorescence1
For a photochromic spiro-diarylethene derivative (Spiro-DAE), its photocyclization reaction and multicolour fluorescence modulation performances were investigated based on a series of density functionals calculations in this work. (academic-accelerator.com)
Nitrogen3
Hydrazones, which are electron-rich compounds, react with diazonium salts either at a nitrogen or a carbon atom to produce formazans. (wikipedia.org)
These data were obtained through the measurement of the level of nitrogen substrate utilization by microorganisms, i.e. the respiratory response of the tested isolates resulting from substrate catabolism (colorimetric reaction) (490 nm) and the level of cell biomass growth (measured by optical density) (750 nm). (edu.pl)
A2_nutrition_D F E - raw data regarding research results to evaluate fungi's use of chromogenic compounds (D F E) as carbon and nitrogen sources. (edu.pl)
Derivatives3
The formazans are compounds of the general formula [R-N=N-C(R')=N-NH-R"], formally derivatives of formazan [H2NN=CHN=NH], unknown in free form. (wikipedia.org)
Formazans can also be produced by the oxidation of the corresponding hydrazidines, usually prepared via reaction of hydrazonyl halides with the appropriate hydrazine derivatives. (wikipedia.org)
Results: The GH5_5 catalytic domain from Orciraptor showed pronounced activity on soluble cellulose derivatives and mixed-linkage glucans, with reaction optima comparable to known GH5_5 representatives. (megazyme.com)
Yield2
Under basic conditions, ethyl nitrate reacts at the methylene position to yield 3-methyl-1,5-diphenylformazan, which can also be obtained from the reaction of phenylazoethane with isoamyl nitrite. (wikipedia.org)
Furthermore, the color yield provided by the reaction product produced by the subject analogs is often greater than that observed for ANS in many embodiments of the subject invention, where when the color yield is greater, the color yield can be at least 5 % greater, usually at least about 20 % and in many embodiments at least about 35 % greater. (allindianpatents.com)
Ultraviolet1
The compound has an unusual ultraviolet spectrum, and the color change can be followed quantitatively by measuring changes in absorption which occur in the 380- to 500-nm region, where cephalosporins normally have no absorption. (nih.gov)
Organisms1
β-galactosidase can catalyze two different reactions in organisms. (wikidoc.org)
Derivative1
Remarkably, carcinogenic benzidine was replaced by its isomeric tetramethyl derivative, the 3,3', 5,5'- tetramethylbenzidine (TMB), which presents several advantages: it is cheap, not mutagenic and a ready-to-use chromogenic substrate. (bvsalud.org)
Measurement1
Substrate for the specific measurement of endo -1,4-β-D-glucanase (cellulase). (megazyme.com)
Results2
Oxidation of such compounds results in their conversion into colorless tetrazolium salts. (wikipedia.org)
A5_toxicity_MTT INT - raw data regarding research results to evaluate the toxicity of chromogenic compounds ( MTT INT ) for fungi. (edu.pl)
Conditions2
Diazonium salts add to active methylene compounds to form an intermediate azo compound, followed by the addition of a second diazonium salt (under more alkaline conditions), yielding tetrazene, which then forms a 3-substituted formazan. (wikipedia.org)
The use of versatile intermediates is important for oligosaccharide syntheses as is the choice for suitable protective and de-protective groups that can hold up against a variety of reaction conditions. (tcichemicals.com)
Form3
Another form to synthesize formazans is by the reaction of active methylene compounds with diazonium salts. (wikipedia.org)
When reduced in a cell, either enzymatically or through direct reaction with NADH or NADPH, the classical tetrazolium salt, MTT, turns blue to purple and may form an insoluble precipitate. (wikipedia.org)
After dyeing, the substrates were either soluble or could be readily dispersed to form fine, gelatinous suspensions. (megazyme.com)
Activity3
Screening of a collection of 450 compounds as inhibitors revealed a structure-activity relationship that mimics in part that of mammalian DPP9. (inra.fr)
PON1 can be evaluated by its different activities , for example its paraxonase activity (when paraoxon is used as substrate), arylesterase activity (when a non phosphorous arylester such as phenyl acetate or 4 (p)-nitrophenyl acetate is used as substrate) or by its lactonase activity (when 5-thiobutil butyrolactone -TBBL- or other lactones such as dihydrocoumarin are used as substrate). (biomedcentral.com)
Maybe it would be more appropriate to use the general term "PON1 activity" that can be measured by different substrates such as paroxon, phenyl acetate, 4-nitrophenyl acetate, TBBL or dihydrocoumarin. (biomedcentral.com)
Produce1
The reaction of diazonium compounds with aldehyde hydrazones is one of the most common procedures to produce formazans. (wikipedia.org)
Sample3
5. Wash the sample with PBS several times to stop the staining reaction. (dojindo.com)
These methods also ignore synergistic effects from compound mixtures and can underestimate toxic potential of a sample. (biotoxicity.com)
Consequently, higher levels of LMWH or UH in the sample lead to lower chromogenic intensity. (medscape.com)
Product1
By "reduced drift" is meant that the reaction product produced upon combination of the subject ANS analog with MBTH (or an analog thereof) has a more stable reading compared with the reading drift observed upon reaction of ANS with MBTH, or the same MBTH analog, where the drift is measured over a period of 2 minutes from the start of the reaction. (allindianpatents.com)
Blue2
They have a variety of colors from dark blue to deep red to orange, depending on the original tetrazolium salt used as the substrate for the reaction. (wikipedia.org)
The hydrogels used as a printing paste were composed of water, poly(ethylene oxide)- block -poly(propylene oxide)- block -poly(ethylene oxide) (Pluronic F-127) as a gel matrix and nitro blue tetrazolium chloride as a radiation-sensitive compound. (envite.org)
Method1
The method of preparation can also be applied to obtain substrates for other endo-hydrolases. (megazyme.com)
Medium1
Lithium chloride in the base medium and supplementary antimicrobial compounds ceftazidime, polymyxin, nalidixic acid, and cycloheximide provide the medium's selectivity. (thermalindo.com)
CASE1
Immunohistochemistry or immunohistochemical staining is a specific use case of immunostaining when the antigen - antibody reaction is used to study the status of molecules in tissue (from the Greek histos , which means tissue). (technologynetworks.com)
Data1
1963) Tables of Spectrophotometric Absorption Data of Compounds Used for the Colorimetric Determination of Elements. (photochemcad.com)
Cell1
Two recently identified exo-lytic N -acetylmuramidases (Tf_NamZ1 and Tf_NamZ2) specifically cleave the peptide-free, exogenous (nutrition source) PGN in the periplasm and release the MurNAc and disaccharide substrates for the transporters Tf_MurT and Tf_AmpG, respectively, whereas the peptide-containing, endogenous (the self-cell wall) PGN stays unattached. (karger.com)