Chromatography protein electrophoresis high performance liquid chromatography chromatography gel. Medical search. Frequent questions

The trimeric conformation and purity of the modified spike protein were tested using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and size-exclusion high-performance liquid chromatography (HPLC). (news-medical.net)
Furthermore, analysis of TolC-null mutations using high performance liquid chromatography (HPLC) reveals that the TolC mutant secretes little, if any, enterobactin. (umsystem.edu)
This protein profiling method using gel electrophoresis coupled with nano-HPLC/ESI/MS/MS and manual evaluation is a sensitive and accurate method for protein identification as well as a powerful tool for monitoring various types of food products. (elsevierpure.com)
This include topics on Thin Layer Chromatography (TLC), High Performance Liquid Chromatography (HPLC), Gas Chromatography Mass Spectrometry (GC-MS), Liquid Chromatography Mass Spectrometry (LS-MS), and Affinity Chromatography. (elac.edu)
Twenty-one species belonging to 13 genera of Acanthaceae obtained from Saudi Arabia were studied using electrophoretic analysis for protein patterns and two isoenzymes polymorphism in addition the phytochemical screening by TLC and HPLC. (scholarsresearchlibrary.com)
As well, fourteen phenolic acids have been screened by high-performance liquid chromatography (HPLC) method. (scholarsresearchlibrary.com)
The main objective of this study is to clarify the relationships among 21 taxa related to two Acanthaceae subfamilies through polyacrylamide gel electrophoresis (PAGE) and high-performance liquid chromatography (HPLC) and to discuss whether these characters can provide an additional fundamental tool which helps in the future the explanation of the taxonomic trends at specific and infra-specific level within the family. (scholarsresearchlibrary.com)
Peptides are easier for separation and characterization by high performance liquid chromatography (HPLC) and HLPC-coupled MS. (creative-proteomics.com)
The presence of HMW species, for instance, is frequently determined using size-exclusion chromatography (SEC) coupled with high-pressure liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC), while LMW species are detected using a capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) (reduced and non-reduced) technique. (pharmtech.com)

Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. (wikipedia.org)
Mass spectrometry is an important method for the accurate mass determination and characterization of proteins, and a variety of methods and instrumentations have been developed for its many uses. (wikipedia.org)
The two primary methods used for the ionization of protein in mass spectrometry are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). (wikipedia.org)
The application of mass spectrometry to study proteins became popularized in the 1980s after the development of MALDI and ESI. (wikipedia.org)
In 1987, Koichi Tanaka used the "ultra fine metal plus liquid matrix method" and ionized biomolecules the size of 34,472 Da protein carboxypeptidase-A. In 1968, Malcolm Dole reported the first use of electrospray ionization with mass spectrometry. (wikipedia.org)
These ionization methods have greatly facilitated the study of proteins by mass spectrometry. (wikipedia.org)
Consequently, protein mass spectrometry now plays a leading role in protein characterization. (wikipedia.org)
Mass spectrometry of proteins requires that the proteins in solution or solid state be turned into an ionized form in the gas phase before they are injected and accelerated in an electric or magnetic field for analysis. (wikipedia.org)
Tandem mass spectrometry (MS/MS) is used to measure fragmentation spectra and identify proteins at high speed and accuracy. (wikipedia.org)
By using our newly developed liquid chromatography-tandem mass spectrometry quantification method, we were able to measure the absolute amount of MRP2/Mrp2 in liver tissues and isolated hepatocytes across species. (aspetjournals.org)
Hong, SS, Park, JH & Kwon, SW 2007, ' Determination of proteins in infant formula by high-performance liquid chromatography-electrospray tandem mass spectrometry ', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , vol. 845, no. 1, pp. 69-73. (elsevierpure.com)
MDI-Hb was also digested with trypsin and specific amino acid conjugation sites determined by ultra-performance liquid chromatography-quadruple-tandem time-of-flight mass spectrometry. (cdc.gov)
For preparative gels, the system was modified to produce the typical protocol compatible with mass spectrometry analysis (17). (gpr120inhibitor.com)
The protein spots whose normalized volumes changed a lot more than 1.5 fold and with P 0.05 have been picked up in the gels that had been stained with all the mass spectrometry compatible system.three.5. (gpr120inhibitor.com)
In-gel Digestion and Liquid ChromatographyTandem Mass Spectrometry AnalysisIn-gel digestion was carried out as previosly described (18). (gpr120inhibitor.com)
For liquid chromatography-tandem mass spectrometry evaluation, the lyophilized samples have been resuspended in 0.1 formic acid before evaluation. (gpr120inhibitor.com)
An Agilent 1100 LC/ MSD trap XCT was utilised for high-performance liquid chromatography and tandem mass spectrometry. (gpr120inhibitor.com)
Protein digestion is a crucial step prior to mass spectrometry (MS) analysis of peptides for successful protein identification and characterization, biomarker discovery, and systems biology. (creative-proteomics.com)
Advances in mass spectrometry are, however, enabling more detailed analyses and thus improved monitoring of residual proteins during downstream processing. (pharmtech.com)
Example MeCAT Workflow Ultra sensitive absolute and relative MeCAT protein quantification is performed by ICP-MS (inductively coupled plasma mass spectrometry) on protein level down to low attomol range. (mecat.eu)
ICP-MS (inductively coupled plasma mass spectrometry) allows an ultra sensitive (absolute) quantification of the metals (lanthanides) tagged to proteins on protein level . (mecat.eu)
If the coding sequence of the protein is unknown but the protein is purified, mass spectrometry (or chemical methods such as Edman degradation) can be used to determine the amino acid sequence of the protein, which can then be used to search for the the corresponding DNA sequence. (hideyuki-ts.com)
At ASDRP, he and his research students are working on a number of high profile biotechnology and synthetic biology research projects, including development of mRNA vaccines for tropical viruses and using metabolomics by mass spectrometry to identify bioactives from natural sources. (asdrp.org)
Detection of tetrahydrocannabinol (THC), cannabidiol (CBD), their major metabolites, and a variety of synthetic cannabinoids by liquid chromatography/mass spectrometry. (tamu.edu)
Detection of anabolic steroids by liquid chromatography/mass spectrometry. (tamu.edu)
Detection of performance enhancing beta-agonist drugs by liquid chromatography / mass spectrometry. (tamu.edu)

Its applications include the identification of proteins and their post-translational modifications, the elucidation of protein complexes, their subunits and functional interactions, as well as the global measurement of proteins in proteomics. (wikipedia.org)
The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts-a critical and often neglected step in successful protein purification. (cshlpress.com)
A proteomics approach revealed that several proteins in the endoplasmic reticulum, the site of N -glycoprotein synthesis, are down-regulated upon apratoxin A exposure. (aspetjournals.org)
Improving the precision of quantitative bottom-up proteomics based on stable isotope-labeled proteins. (uni-frankfurt.de)
At Creative Proteomics, we provide both in-gel and in-solution protein digestion services for protein analysis. (creative-proteomics.com)
Creative Proteomics provides in-gel and in-solution protein digestion services for successful protein and proteome analysis. (creative-proteomics.com)

Mass analysis of proteolytic peptides is a popular method of protein characterization, as cheaper instrument designs can be used for characterization. (wikipedia.org)
This paper describes results on the association between protein kinase A and peptides corresponding to wild-type PLN (residues 8-22) and peptides bearing mutations (R9C and A11E) which in the context of full-length PLN (52 residues) result in dilated cardiomyopathy. (elifesciences.org)
Whey proteins are rich in bioactive peptides, possessing bioactive properties such as being antioxidant and antihypertensive as well as having antimicrobial activities, which, when ingested, confers several health benefits. (encyclopedia.pub)
Milk provides a wide range of biologically active components such as bioactive proteins and peptides, oligosaccharides, immunoglobulins, and fats/lipids that protect against pathogens and illnesses on regular consumption. (encyclopedia.pub)
These properties are because of the presence of milk proteins/peptides, which support infant development, its growth, and confers health benefits beyond basic nutrition [ 2 ] . (encyclopedia.pub)
Protein digestion presents the process to cut proteins into shorter fragments, known as peptides. (creative-proteomics.com)
Although it is possible for MS to study intact proteins, the smaller peptides facilitate protein identification and improve the coverage of proteins that might be reduced due to solubility and heterogeneity. (creative-proteomics.com)
MeCAT labeled peptides and proteins are separated by 2D electrophoresis, PAGE or LC followed by ICP-MS quantification. (mecat.eu)

Oligosaccharide fingerprinting employing capillary zone electrophoresis. (chipsbooks.com)

However, because of the unknown binding affinity of antibody to MRP2/Mrp2 protein across species and lack of purified MRP2/Mrp2 proteins for calibration, the immunoblotting assay was excluded from the absolute quantification of MRP2/Mrp2 protein for multiple species. (aspetjournals.org)
Affibody molecules hold great promise as carriers of cytotoxic drugs for cancer therapy due to their typically high affinity, easy production, and inherent control of the drug molecules' loading and spatial arrangement. (mdpi.com)
Inhibition of the enzyme by 8-azaguanine and azathioprine provides a starting point for the synthesis of higher affinity purine-based inhibitors. (biomedcentral.com)

Techniques include protein isolation, purification and characterization, protein assays, immunoassay: Enzyme-Linked Immunosorbent Assay (ELISA), Western Blot, Sodium Dodecyl Sulfate Polyacrylamide (SDS-PAGE), agarose gel electrophoresis, and Polymerase Chain Reaction (PCR) technology. (elac.edu)
Identification of United States Barley Cultivars by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis of Hordeins. (cerealsgrains.org)
LPS samples isolated from different mutants carrying mutations in the 3.9-kb SmaI-XhoII DNA fragment exhibited banding patterns in silver-stained sodium dodecyl sulfate-polyacrylamide gels markedly different from that of the wild-type LPS. (uni-bielefeld.de)

Oligosaccharide fingerprinting employing high performance anion exchange liquid chromatography with pulsed amperometric detection (HPAE-PAD). (chipsbooks.com)
Moreover, comparison of the monosaccharide composition obtained by high-performance anion-exchange chromatography with pulsed amperometric detection of LPS purified from wild-type Xanthomonas campestris pv. (uni-bielefeld.de)

MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross-linked polyacrylamide gels. (uni-frankfurt.de)
Advantageously is the use of the breakable polyacrylamide gels DPAGE (disolvable PAGE) of Proteome Factory because protein gel spots or bands can easily liquefied (fluidized) after protein separation and staining for FIA-ICP-MS analysis. (mecat.eu)

Gel images were analyzed by Prognosis computer software (Nonlinear, Newcastle-upon-Tyne, UK) as outlined by the instruction procedure for differentially expressed proteins. (gpr120inhibitor.com)
Functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis resulted in the differentially expressed proteins in the FM and OA groups are mostly involved in the coagulation and complement system-related pathways, serotonin pathways, dopamine pathways, glutamate pathways and acute phase reactions. (ncl.edu.tw)

The quality of the PCR product was confirmed using agarose gel electrophoresis. (umsystem.edu)
The strips have been sealed on best of a 12.five sodium dodecyl sulfate gel employing 0.five agarose. (gpr120inhibitor.com)

Finally, there is a new chapter on a method complementary to gel electrophoresis and chromatography: in silico analysis of genomic and proteomic databases. (cshlpress.com)
Experimental as well as in silico analysis inferred that the host protein causes not only change in the integration events but also targets the incoming viral DNA or the integrase-viral DNA complex. (biomedcentral.com)

Whey is a yellow-green colored liquid portion of milk, also called cheese serum is obtained after separation of the curd, during coagulation of milk using proteolytic enzymes or acids [ 12 ] . (encyclopedia.pub)
Compatible with common protein and peptide separation technologies (electrophoresis, LC etc. (mecat.eu)

Most symptomatically infected pa- by pulsed-fi eld gel electrophoresis (PFGE), a technique that tients have painful bloody diarrhea ( 2 , 3 ). (cdc.gov)

In a recent study published in the journal npj Vaccines , researchers in China designed, produced, and evaluated the neutralization efficacy of a recombinant coronavirus disease 2019 (COVID-19) vaccine containing four hot-spot substitution mutations based on a prefusion-stabilized severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S) trimer. (news-medical.net)
HCPs are generated during the production of recombinant proteins. (pharmtech.com)
When the rare tRNAs are depleted to produce the recombinant protein, proliferation of the host cells is restricted, leading to low yield. (hideyuki-ts.com)

This course focuses on protein and nucleic acid chemistry. (elac.edu)
The nCounter® SPRINT Profiler (manufactured by NanoString Technologies, Inc.) allows simultaneous quantitative analysis of up to 800 nucleic acid sequences (DNA and RNA) or proteins in a sample (or simultaneous quantitative analysis of up to 200 nucleic acid sequences in 4 samples). (elixir-slovenia.org)
SeqStudio is an automated capillary electrophoresis system for nucleic acid analysis. (elixir-slovenia.org)

These ionization techniques have played a significant role in the characterization of proteins. (wikipedia.org)
It allows for the identification and characterization of proteins according to their properties. (creative-proteomics.com)
Facilitate protein identification and characterization, discover disease biomarkers, and contribute to the understanding of biological processes. (creative-proteomics.com)
The invention regards a method which enables high reproducible systematic, qualitative and quantitative characterization of proteomes and biomolecules. (mecat.eu)

SDS-PAGE assay with His-tag In-gel stain revealed TolC expression. (umsystem.edu)
The trinitrobenzene sulfonic acid assay (TNBS) and denaturing gel electrophoresis were used to determine the extent of cross-linking. (cdc.gov)
Subsequently, protein concentration on the depleted sera was determined by a Bradford protein assay, working with albumin as the regular.3.3. (gpr120inhibitor.com)
In the present study, we have identified a new HIV-1 integrase interacting protein, polypyrimidine tract binding protein and associated splicing factor (PSF) through pull down assay and co immunoprecipitation of IN with mammalian cell protein. (biomedcentral.com)

It can also be used to localize proteins to the various organelles, and determine the interactions between different proteins as well as with membrane lipids. (wikipedia.org)
Moreover, a significant loss of Mrp2 protein in the membrane fraction of rat cryopreserved hepatocytes was observed. (aspetjournals.org)
Allophycocyanin (AP, λ max = 650 nm) forms the core structure, connecting the phycobilisomes to the thylakoid membrane via linker proteins. (biomedcentral.com)

These strategies are designed to solubilize hydrophobic proteins, digest protein with trypsin or other proteases, and remove SDS. (creative-proteomics.com)

The World Health Organization (WHO) has classified the SARS-CoV-2 Alpha, Beta, Gamma, Delta, and Omicron variants as variants of concern because they carry mutations in their spike protein that increase their transmissibility and ability to evade vaccine-induced immunity, as compared to the ancestral Wuhan strain. (news-medical.net)
The present study used the HexaPro (S-6P) S-trimer and an Alum/cytosine phosphoguanine (CpG) 7909 dual adjuvant system to create a modified spike trimer vaccine containing the four spike protein substitution mutations commonly found in the five variants of concern. (news-medical.net)

In addition to quantification of MeCAT metal tagged proteins other elements like phosphorus, sulfor, selenium (metalloproteins) can detected, for example for verification and accurate absolute quantification of phospho proteins (see below). (mecat.eu)

Comparison of Polyacrylamide Gel Electrophoresis and High-Performance Liquid Chromatography Analyses of Gliadin Polymorphism in the Wheat Cultivar Newton. (cerealsgrains.org)

Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. (cshlpress.com)
MDI conjugation was observed to be dependent on the MDI: protein ratio and the concentration of protein. (cdc.gov)
For proteins in solution, the concentration should be greater than 1 mg/mL. (creative-proteomics.com)
Patients with T2D had higher C-reactive protein (CRP) and monocyte chemotactic protein-1 (MCP1) levels and lower adiponectin concentration, compared to controls. (biomedcentral.com)

Oligosaccharide fingerprinting employing capillary gas chromatography with flame ionization detection. (chipsbooks.com)
As a result, CE-SDS is used for the detection of LMW protein impurities. (pharmtech.com)

For crystallography studies, a protein sample with high purity, solubility and yield must be obtained. (hideyuki-ts.com)

The variant's effect on the function of the gene/protein, displayed in the format 'R/C'. R is the value reported by the source (publication, submitter) and this classification may vary between records. (gob.ar)
The host cells used for fermentation/cell culture produce HCPs necessary for the cells to function (growth, gene transcription, protein synthesis, etc.) and during cell death. (pharmtech.com)

The silver-stained gels had been scanned making use of a GS-800 calibrated densitometer (Bio-Rad) at 300 dpi. (gpr120inhibitor.com)

The most common protein-based impurities that downstream processes are developed to control include high- and low-molecular-weight species, charge-variants, and host-cell proteins. (pharmtech.com)
In high-throughput approaches, such as the pipeline adopted by Oxford Protein Production Facility (OPPF), many variants are constructed in parallel to maximise output. (hideyuki-ts.com)

A proteome analysis of mammalian glomeruli was performed in mouse using two-dimensional-gel electrophoresis with separate Coomassie and silver staining and subsequent mass spectrometric identifications. (researchgate.net)

In electrospray, the ions are created from proteins in solution, and it allows fragile molecules to be ionized intact, sometimes preserving non-covalent interactions. (wikipedia.org)

Electrospray ionization of a protein often results in generation of multiple charged species of 800 (wikipedia.org)
The cross-reactions of MRP2/Mrp2 protein of different species with anti-human MRP2 polyclonal antibody were found by Western blotting. (aspetjournals.org)
The amount of Mrp2 in rat liver was approximately 10-fold higher than that in other species. (aspetjournals.org)
We recommend that despite high initial costs, for long-term demographic monitoring of low-density species, or where logistical constraints make traditional field monitoring inaccurate, remote monitoring options should be considered. (bvsalud.org)
A total number of twenty-three protein bands observed between the species ranged 10 and 250 K Da, nine esterase and six peroxidase polymorphisms were detected. (scholarsresearchlibrary.com)
The biochemical data (protein profiles and isoenzymes polymorphisms) considered a significant tools for the relationships between the studied species. (scholarsresearchlibrary.com)
Residual undesired proteins-host-cell proteins (HCPs) and high-molecular-weight (HMW) and low-molecular-weight (LMW) species-have the potential to affect the safety and efficacy of biopharmaceuticals. (pharmtech.com)
Since human demand for good traits and yield is very high, only a small fraction of the world's approximately 200,000 plant species have, through history, survived the rigorous scrutiny of the domestication process. (hindawi.com)

Whole-protein mass analysis is primarily conducted using either time-of-flight (TOF) MS, or Fourier transform ion cyclotron resonance (FT-ICR). (wikipedia.org)
The most widely used instrument for peptide mass analysis are the MALDI-TOF instruments as they permit the acquisition of peptide mass fingerprints (PMFs) at high pace (1 PMF can be analyzed in approx. (wikipedia.org)
The complexity and sheer number of proteins in a cell are impediments to identifying proteins of interest or purifying proteins for function and structure analysis. (cshlpress.com)
Quantitative Analysis of Soybean Proteins by Densitometry on Gel Electrophoresis. (cerealsgrains.org)
Our technologies allow complete analysis of bulk drug substance and liquid or lyophilized drug product, and the validated analytical methods are performed under rigorous quality oversight, following defined standard operating procedures. (biodextris.com)
Using a microscope and computer image analysis, the system determines the type and number of bound probes and thus enables the quantitative analysis of nucleic acids and proteins. (elixir-slovenia.org)

The expression and purification strategies depend on the characteristics of the protein of interest, such as size, shape, intrinsic solubility, requirement of post-translational modifications and/or cofactors, presence of disulfide bridges, in vitro stability. (hideyuki-ts.com)

The SDS denatures the protein, and if needed a reducing agent can be added to break any disulfide bonds. (pharmtech.com)

The mobile phase A of liquid chromatography was water/0.1 formic acid plus the mobile phase B was acetonitril/0.1 formicacid. (gpr120inhibitor.com)

It mainly forms the catalytic subunits of two protein complexes, including mTOR complex 1 (mTOR complex1, mTORC1) and mTOR complex 2 (mTOR complex2, mTORC2). (hindawi.com)

The "self"-protein albumin in mouse airway fluid was uniquely modified by GSH-MDI at position 414K, a preferred site of MDI reactivity on human albumin. (cdc.gov)

In MALDI, the proteins are embedded within a matrix normally in a solid form, and ions are created by pulses of laser light. (wikipedia.org)
Electrospray produces more multiply-charged ions than MALDI, allowing for measurement of high mass protein and better fragmentation for identification, while MALDI is fast and less likely to be affected by contaminants, buffers and additives. (wikipedia.org)
Proteins can be identified and relatively quantified on peptide level by MALDI-MS an ESI-MSMS. (mecat.eu)

Experimental research found a markedly decreased expression of autophagy-related proteins such as Beclin-1, Atg12-Atg5, and LC3-II in a diabetic mouse model and in a high glucose-induced podocyte injury model [ 4 ]. (hindawi.com)

The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. (cshlpress.com)
The different interactions between viral proteins and cellular host proteins are required for efficient replication of HIV-1. (biomedcentral.com)

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , 845 (1), 69-73. (elsevierpure.com)
The gels were visualized by utilizing a complete protocol of a silver staining strategy for analytical gels. (gpr120inhibitor.com)
The properties of the different types of residual protein contaminants in biologic drugs vary significantly, leading to the need to use different analytical methods to detect and monitor each. (pharmtech.com)
We, at Biodextris, validate your assays to demonstrate that the appropriate performance criteria are met, and the analytical target profile is achieved. (biodextris.com)

In addition, the identification and quantification of some of these residual proteins, particularly in the presence of large concentrations of the product protein, can be challenging. (pharmtech.com)
Identification of these interacting host cellular protein provide better understanding of mechanism of viral replication and subsequently development of new therapeutic approaches. (biomedcentral.com)

The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. (researchgate.net)

In summary, TSN reduces podocyte damage induced by high glucose via inhibiting mTORC1 pathway and downstream targets and restoring podocyte autophagy. (hindawi.com)

Intracellular iron levels are elaborately balanced by iron efflux, uptake, and storage proteins that are regulated by iron regulatory proteins (IRPs, including IRP1 and IRP2) [ 16 ]. (nature.com)
PLN interacts with sarco/endoplasmic reticulum Ca 2+ -ATPase (SERCA) and regulates calcium uptake, which is modulated by the protein kinase A (PKA)-dependent phosphorylation of PLN during the fight-or-flight response. (elifesciences.org)

A cell culture facility equipped with a biosafety cabinet, CO2 incubator, high-speed centrifuge, automated cell counter, and a liquid N2 cryopreserver is also available adjacent to MCBF that supports cell and tissue culture-related research activities. (iitgn.ac.in)

Liquid-phase processing is one of the most convenient and utilized methods of synthesis. (degruyter.com)

Additionally, sample preparation is easier once whole proteins have been digested into smaller peptide fragments. (wikipedia.org)

Metal standard solutions are used for high accuracy external protein independ calibration of ICP-MS. (mecat.eu)

In an attempt to study the host interacting protein of IN, we have identified a new interacting host protein PSF which is a splicing factor and elucidated its role in integration and viral replication. (biomedcentral.com)

Protein-based methods. (chipsbooks.com)
Gel-based methods. (chipsbooks.com)
Multiple methods are required for detecting and removing protein impurities. (pharmtech.com)
By combining the high variation of mutagenised populations with novel screening methods, traits that are almost impossible to identify by conventional breeding are now being developed and characterised at the molecular level. (hindawi.com)
Outline an expression and purification strategy, using both traditional and high-throughput methods, to produce a sample suitable for protein crystallography. (hideyuki-ts.com)
Therefore the functions of nucleomorph-encoded proteins must be elucidated indirectly by application of methods in genetically accessible organisms. (biomedcentral.com)

Milk whey proteins are one of the most valued constituents due to their nutritional and techno-functional attributes. (encyclopedia.pub)
In addition to their bioactivities, whey proteins are known to possess enhanced functional attributes that allow them to be utilized in broad applications, such as an encapsulating agent or carrier materials to entrap bioactive compounds, emulsification, and in edible and active packaging. (encyclopedia.pub)
Besides, proteins extracted from milk are well characterized for their multiple functional characteristics and are utilized by several industries in food applications. (encyclopedia.pub)
This review focuses on exploiting the bioactive and functional properties of milk whey proteins. (encyclopedia.pub)

The structure of some homologous proteins might have been solved previously and we can refer to their expression and purification strategies, which might prevent some waste of time on trial-and-errors. (hideyuki-ts.com)

Greater binding to HSA than Hb was observed and MDI bound to only eight binding sites on Hb compared to twenty for HSA (at 40:1 molar ratio of MDI: protein). (cdc.gov)

In general, the proteins are analyzed either in a "top-down" approach in which proteins are analyzed intact, or a "bottom-up" approach in which protein are first digested into fragments. (wikipedia.org)

Self-polymerization of MDI onto protein was observed on some amino acids at higher MDI concentrations. (cdc.gov)
The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. (researchgate.net)

The deduced amino acid sequences of these ORFs showed a high degree of homology to the deduced amino acid sequences of the rfbC, rfbD, rfbA, and rfbB genes of Salmonella typhimurium LT2, which have been shown to encode a set of enzymes responsible for conversion of glucose 1-phosphate to dTDP-rhamnose. (uni-bielefeld.de)

Anatomy12

Organisms15

Diseases3

Chemicals and Drugs109

Analytical, Diagnostic and Therapeutic Techniques and Equipment63

Phenomena and Processes46

Disciplines and Occupations7

Technology, Industry, Agriculture1

Information Science4

Health Care8

HPLC9

Mass spectrometry26

Proteomics6

Peptides8

Capillary zone electropho1

Affinity3

Sodium Dodecyl Sulfate Polyac3

Anion exchange2

Polyacrylamide gels2

Differentially expressed proteins2

Agarose2

Silico analysis2

Separation2

PFGE1

Recombinant3

Nucleic acid3

Characterization4

Assay4

Membrane3

Hydrophobic1

Mutations2

Absolute quantification1

Analyses1

Concentration4

Detection2

Purity1

Gene2

Silver-stained1

Variants2

Proteome analysis1

Molecules1

Species8

Analysis6

Post-translational1

Disulfide1

Formic1

Complexes1

Albumin1

MALDI3

Markedly1

Interactions2

Analytical4

Identification2

Proteomic1

Targets1

Uptake2

Incubator1

Synthesis1

Peptide1

Calibration1

Viral1

Methods6

Functional4

Purification1

Molar1

Fragments1

Concentrations2

Amino acid1