Chromatographic separation media stationary phase. Medical search. Frequent questions

At industrial scale an SMB chromatographic separator is operated continuously, requiring less resin and less solvent than batch chromatography. (wikipedia.org)
We studied the determination of phenylpropionylglycine in urine by isocratic ion-exclusion chromatography on a cation-exchange column using water-sulphuric acid (pH values between 2 and 4) as mobile phase. (nih.gov)
We conclude that, even in asymptomatic patients, determination of phenylpropionylglycine in urine after a phenylpropionic acid load by ion-exclusion chromatography is a rapid and reliable diagnostic tool for the detection of medium-chain acyl-CoA dehydrogenase deficiency. (nih.gov)
Anion exchange chromatography (AEX) exploits the negative charge on EV surface molecules for binding to the positively charged solid phase. (biaseparations.com)
Chromatography is the separation of mixtures into their components as a result of differential distribution of the solutes in order for identification, analyses, purify and/or quantity of the mixture for a mobile medium and for a stationary absorbing medium. (majortests.com)
What is the basic principle for separation in normal phase, reversed phase, ion-exchange and gel permeation chromatography? (majortests.com)
Normal and Reversed Phase Chromatography What is the relationship between polarity of the mobile phase vs. elution time and resolution in normal phase and reversed phase chromatography? (majortests.com)
Hydrophobic interaction chromatography (HIC) uses a filler with moderate hydrophobicity as the stationary phase and a salt-containing aqueous solution as the mobile phase, and uses the difference in the hydrophobic properties of the solute molecules to achieve separation by the difference in the strength of the hydrophobic interaction with the stationary phase, chromatographic method. (welch-us.com)
Because the separation principle of hydrophobic interaction chromatography is completely different from chromatographic techniques such as ion exchange chromatography or gel filtration chromatography, this technique is often used in combination with the latter two to separate complex biological samples. (welch-us.com)
In theory, HIC and RPC are two closely related liquid chromatography techniques, both of which are based on the flow-water interaction between the hydrophobic regions on the surface of biomolecules and the hydrophobic ligands (alkyl or aromatic) on the chromatographic medium , however, the chromatographic mechanisms at the molecular level as well as the practical level differ between the two techniques. (welch-us.com)
This leads to the development of novel preparative liquid chromatography in which mobile phase flows down by positive air pressure called as Flash chromatography. (ijpsr.com)
In flash chromatography however air pressure is used to speed up the flow of solvent, dramatically decreasing the time needed to purify the sample, therefore making the column and running the separation could take less than 10-15 minutes. (ijpsr.com)
Flash chromatography is basically an air pressure driven hybrid of medium pressure and shorter column chromatography which has been optimized for particularly rapid separation. (ijpsr.com)
Flash chromatography differs from the conventional technique in two ways: first, slightly smaller silica gel particles (250-400 mesh) are used, and second, due to restricted flow of solvent caused by the small gel particles, pressurized gas ( ca. 10-15 psi) is used to drive the solvent through the column of stationary phase. (ijpsr.com)
MPLC - Medium pressure liquid chromatography (MPLC) systems which operate above 150 psi. (ijpsr.com)
The stationary phase is also commonly known as the packing, which is the core part of chromatography. (flashcolumns.com)
After modification, it has a wide range of applications in the separation and purification of biomolecules such as proteins and carbohydrates in ion exchange chromatography, size exclusion chromatography, and reversed-phase hydrophobic chromatography. (flashcolumns.com)
The chromatographic mode in which the polarity of the modified groups on the matrix is greater than the polarity of the mobile phase is called normal phase chromatography, which relies on the different polarity of the sample to distribute between the stationary phase and the mobile phase to achieve separation. (flashcolumns.com)
High-performance liquid chromatography has become the predominant global separation technique over the past four decades. (chromatographytoday.com)
What does the mobile phase do in chromatography? (profound-answers.com)
Chromatography relies on two different 'phases': the mobile phase is the solvent that moves through the paper, carrying different substances with it. (profound-answers.com)
Pure water in elevated temperature can be used as mobile phase in liquid chromatography mainly due to change of dielectric constant. (profound-answers.com)
chemistry) The liquid or gas that flows through a chromatography system, moving the materials to be separated at different rates over the stationary phase. (profound-answers.com)
What makes a good mobile phase in chromatography? (profound-answers.com)
What role does the mobile phase play in the distance a molecule travels in chromatography? (profound-answers.com)
The molecule phase displays the solvent used in chromatography. (profound-answers.com)
What do you mean by mobile phase and stationary phase in chromatography? (profound-answers.com)
In all chromatography there is a mobile phase and a stationary phase. (profound-answers.com)
In paper and thin-layer chromatography the mobile phase is the solvent. (profound-answers.com)
Which is the mobile phase of liquid chromatography? (profound-answers.com)
Liquid chromatography (LC) is a common purification technique with liquid mobile phases. (amerigoscientific.com)
High performance liquid chromatography (HPLC) columns use higher pressures and smaller solid particles, which not only overcome some limitations of standard LC, but also allow for more effective separations and wider applications, such as pharmaceutical factory, environment, forensics, and chemical industry. (amerigoscientific.com)
We offer monodisperse chromatography media for usage from analytical to process scale separation. (amerigoscientific.com)
Our packings and media mainly consist of silica gels or polymeric resins for normal phase, reversed phase, affinity, ion exchange, and hydrophilic interaction chromatography. (amerigoscientific.com)
Offering the chemist both ease of use and the potential to separate complex sample matrices, it's easy to see why thin layer chromatography is such a popular separation technique. (microsaic.com)
Thin layer chromatography (TLC) is a chromatographic method where the analytes are separated on a solid stationary phase plate, comprising of an adsorbent material coating on top of a sheet of aluminium or plastic. (microsaic.com)
Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. (lookformedical.com)
It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. (lookformedical.com)
A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
From simple separations to detailed wavelength analysis, we examine the important concepts and techniques needed to make liquid chromatography work its best in the laboratory. (cannabissciencetech.com)
Chromatography allows for the separation of a mixture of compounds by diluting the mixture into a mobile phase which then passes through or over a stationary phase to create separation by the interaction and affinity of individual compounds for one of the phases of the chromatography system ( Figure 1a ). (cannabissciencetech.com)
Mobile phase is a solvent or gas that flows through a chromatography system and is also referred to as an eluant . (cannabissciencetech.com)
Stationary phase is a liquid or solid material that is fixed in a chromatography system. (cannabissciencetech.com)
The purpose for separation falls into two categories: preparative chromatography or analytical chromatography. (cannabissciencetech.com)
Chromatography processes are further divided into two major categories (liquid or gas chromatography) based on the composition of their mobile phase component. (cannabissciencetech.com)
Gas chromatography (GC) uses a carrier gas, such as hydrogen or helium, to separate compounds based on their interaction with a stationary phase or based on their boiling points. (cannabissciencetech.com)
In liquid chromatography (LC), compounds are separated by dissolving a sample into a liquid mobile phase which is then passed over a solid stationary phase. (cannabissciencetech.com)
This general chapter applies to chromatographic analytical procedures and supplements the general chapters on Thin-layer chromatography (2.2.7) , Gas chromatography (2.2.28) , Liquid chromatography (2.2.29) and Size-exclusion chromatography (2.2.30) . (edqm.eu)
Schomburg, G. Stationary phases for reversed-phase liquid chromatography: Coating of silica by polymers of various polarities. (mpg.de)
Bien-Vogelsang, U. Immobilization of stationary liquids in reversed- and normal-phase liquid chromatography: Production and testing of materials for bonded-phase chromatography. (mpg.de)
These models offer insight into selectivity trends observed in liquid chromatography with C18 and C30 stationary phases, prepared by monomeric or polymeric synthetic approaches, and operated at different column temperatures. (nist.gov)
In a separate effort, alkyl stationary phases used in liquid chromatography have been modeled by molecular dynamic simulations, under chromatographically relevant conditions. (nist.gov)
2. Lippa, K. A. and Sander, L. C., "Identification of isolated cavity features within molecular dynamics simulated chromatographic surfaces," Journal of Chromatography A, 1128, 79-89 (2006). (nist.gov)
The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples. (lu.se)
UHPSFC , ultra- pacted by other parameters, such as the addition of the mod- high performance supercritical fluid chromatography ifier and the change of the mobile phase density [20]. (lu.se)
The solvent system which gives maximum separation of the spots will be better solvent for column chromatography. (drinksavvyinc.com)
This is an air pressure driven technique comprising of medium and short column chromatography, optimised for rapid separation of organic compounds. (drinksavvyinc.com)
Flash chromatography, also known as medium pressure chromatography, was popularized several years ago by Clark Still of Columbia University, as an alternative to slow and often inefficient gravity-fed chromatography. (drinksavvyinc.com)
Based on the principle of gas-liquid partition chromatography, the technique utilizes different stationary phases packed in a stainless steel column to separate mixtures of volatile compounds. (nighthelper.com)
Packed column gas chromatography (GC) is a powerful and versatile analytical technique that offers numerous benefits, including high-resolution separations, fast analysis times, low cost, and ease of operation. (nighthelper.com)
Spherical polyimide (PI) particles have been introduced as a stationary phase in liquid chromatography (LC). (go.jp)
In this study, we reveal the suppression of non-specific hydrophobic interaction in poly(ethylene-co-glycidylmethacrylate) (PEGM) based spongy monolith (SPM), PEGM-SPM, which has recently be reported as a new platform of separation medium for affinity chromatography in our previous study, by an simple acidic treatment. (go.jp)
A solid-phase extraction-type collection device packed with Sunpak-H, styrene-divinylbenzene polymer particles, was used to identify 16 airborne polycyclic aromatic hydrocarbons (PAHs) using high-performance liquid chromatography (HPLC)-fluorescence detection and ultraviolet detector. (go.jp)
HPAE-PAD - High-Performance Anion Exchange chromatography with Pulsed Amperometric Detection - is a technique that was developed specifically for the separation and detection of carbohydrates. (thermofisher.com)

The present study reports the optimization and validation of a dissolution test for selegiline.HCl tablets using a new high-performance liquid chromatographic (HPLC) method. (shengsci.com)
Dr. Tao Jiang is an expert in HPLC, GC and LC/MS with more than 20 years of research experience in separation media, instrumentation and their applications in pharmaceutical field. (ca-ca.org)
In addition, we provide qualified LC/HPLC accessories, vials, plates and monodisperse media to ensure optimal performance and robust analysis of chromatographic systems. (amerigoscientific.com)
Schomburg, G. Polymer-coating approach for HPLC stationary phases: Efficiency, chemical stability, versatility. (mpg.de)
When developing an HPLC method, the first step is always to consult the literature to ascertain whether the separation has been previously performed and if so, under what conditions - this will save time doing unnecessary experimental work. (pharmtech.com)
for example, if the sample includes polar analytes then reverse phase HPLC would offer both adequate retention and resolution, whereas normal phase HPLC would be much less feasible. (pharmtech.com)
For low/medium polarity analytes, normal phase HPLC is a potential candidate, particularly if the separation of isomers is required. (pharmtech.com)
Reverse phase gradient HPLC is commonly used in peptide and small protein analysis using an acetonitrile-water mobile phase containing 1% trifluoroethanoic acid. (pharmtech.com)
The 2D-HPLC system incorporated strong cation exchange and reversed phase separation modes. (cdc.gov)

The cost reductions come about as a result of: the use of a smaller amount of chromatographic separation media stationary phase, a continuous and high rate of production, and decreased solvent and energy requirements. (wikipedia.org)
SMB provides lower production cost by requiring less column volume, less chromatographic separation media ("packing" or "stationary phase"), using less solvent and less energy, and requiring far less labor. (wikipedia.org)
But these drawbacks are effectively compensated by the better yield and a much lower solvent consumption as well as a much higher productivity compared to simple batch separations. (wikipedia.org)
Separation of dried liquid samples with a liquid solvent and a glass plate covered with a thin layer of silica gel. (majortests.com)
Obviously, the interaction between the hydrophobic solute and the RPC medium is much stronger than that of the HIC medium, and severe elution conditions such as organic solvent gradients are required to elute the solute from the column. (welch-us.com)
Separation of dibromochloromethane and bromoform from biological samples is most often achieved by headspace analysis, purge-and-trap collection, solvent extraction, or direct collection on adsorbent resins. (cdc.gov)
For water-insoluble materials such as fat or other tissues, the most common separation procedure is extraction with an organic solvent such as diethyl ether (Zlatkis and Kim 1976). (cdc.gov)
Solvent extraction and separation tech. (slideshare.net)
Similar a Solvent extraction and separation tech. (slideshare.net)
With Quatro-binary gradient capability traditional binary gradients with a limited polarity range can be adjusted to elute very lipophilic and highly polar compounds within a single purification or compound solubility during the separation by adding a constant amount of a co-solvent, acid, or base with the Isolera advanced pump. (biotage.com)
Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase). (lookformedical.com)
The mixed solvent system served as a solvation and reaction medium for efficiently producing reliable FAB mass spectra and for the partial displacement of the ligands for ease in their identification. (drinksavvyinc.com)
Modern flash chromatographic system consists of pre-packed plastic cartridges wherein solvent is pumped through the cartridges to achieve separation. (drinksavvyinc.com)

This report concerns a benchtop prototype instrument containing a gas chromatographic microanalytical system (uGC) designed for the selective determination of multiple airborne volatile organic compounds (VOCs) at concentrations in the vicinity of recommended occupational exposure limits. (cdc.gov)
It plays a very important role in the separation and purification of compounds. (flashcolumns.com)
1992) used solid phase micro extraction to separate volatile halogenated compounds from water samples. (cdc.gov)
Compounds are identified by their degree of retention on the stationary phase, and compared with a reference-standard spot. (microsaic.com)
Step-gradients can be short and powerful providing separations for one or more compounds but can be challenging to develop and optimize. (biotage.com)
This new development worked with the affinity of the target compounds with the different phases allowing for separation and collection of individual compounds. (cannabissciencetech.com)
Mixtures are heated and vaporized in an injection port and are moved through or across a stationary phase to separate compounds before reaching a detector that provides data on concentration or identity of components. (cannabissciencetech.com)
In neutral and alkaline media, these products were also formed but the equilibrium between these three compounds was readily established. (go.jp)

Chromatographic separation of the enantiomers of α-hydroxyallylphosphonates on chiral stationary phases using sequential UV, polarimetric and refractive index detection. (umsl.edu)
Chromatographic enantioseparation of amino acids using a new chiral stationary phase based on a macrocyclic glycopeptide antibiotic. (mpg.de)

This instalment reviews the various process parameters and raw material attributes that affect glycosylation, as well as the different analytical tools that are used for characterization, with greater emphasis on the chromatographic methods of analysis. (chromatographyonline.com)
We have proven a flow independent separation of mAb charge variants and in this way, a resolution comparable to classical CEX particulate-based analytical columns was achieved in only 6 min analysis time. (biaseparations.com)

The silica gel is the stationary phase, TLC uses a thin uniform layer of silica gel or alumina coated onto a piece of glass, metal or rigid plastic. (majortests.com)
At the same time, the surface of silica gel is easily modified to form other bonded stationary phases. (flashcolumns.com)
There are many kinds of stationary phases modified with a silica gel matrix, and the separation and purification effect is much better than other matrixes. (flashcolumns.com)
However, compared with inorganic substrates such as silica gel, the separation mechanical strength that the organic matrix stationary phase can withstand is lower, and the separation and purification effect is poor, which limits its scope of application in purification. (flashcolumns.com)
The normal phase silica gel stationary phase is mainly used in the preliminary purification of synthetic non-polar or medium-polar intermediates. (flashcolumns.com)

The components with lower adsorption and affinity to stationary phase travel faster when compared to the greater adsorption and affinity with the stationary phase. (profound-answers.com)
Additionally, the immobilization procedures of protein-A toward the PEGM-SPM and the separation conditions for immunoglobulin G (IgG) were optimized for further effective affinity separations. (go.jp)

Besides the availability of preparative tools for separation, combining multiple orthogonal and complementary characterization tools is crucial for defining the EV product of interest. (biaseparations.com)
Schomburg, G. Characterization and stability of silanized and polymercoated octadecyl reversed phases. (mpg.de)

It cannot provide any separation or purification that cannot be done by a simple column purification. (wikipedia.org)
The single advantage which it brings to a chromatographic purification is that it allows the production of large quantities of highly purified material at a dramatically reduced cost. (wikipedia.org)
At present, the main application field of this technology is in the purification of proteins, and it has become an effective means for the separation of serum proteins, membrane-bound proteins, nuclear proteins, receptors, recombinant proteins, etc., as well as some drug molecules, and even cells. (welch-us.com)
Today HAWACH will talk about the connotation of the purification column-stationary phase. (flashcolumns.com)
To determine the stationary phase packed in the Flash purification column, three aspects of the stationary phase must be determined in sequence: matrix type, surface modification/bonding phase, and matrix parameters. (flashcolumns.com)
It is used in normal-phase purification systems. (flashcolumns.com)
In addition, when applied to the separation and purification of some biomolecule samples, it will cause non-specific adsorption of samples, or denature the sample, resulting in the deterioration of the peak shape of the sample elution in the chromatogram and the decrease of the sample recovery rate. (flashcolumns.com)
However, it is difficult to modify the surface of alumina, so it is still used in normal phase purification systems at most and a small amount in ion exchange systems. (flashcolumns.com)
The composite matrix is compatible with and neutralizes the advantages and disadvantages of the inorganic matrix and the organic matrix, but the corresponding stationary phase of the composite matrix is generally expensive, so it is less used in preparation and purification. (flashcolumns.com)
In this separation mode, the sample is eluted from the purification column in order from weak to strong polarity. (flashcolumns.com)

Moreover, the obtained MNPs showed the specific extraction efficiency to organic pollutant, polycyclic aromatic hydrocarbons, while superparamagnetic property of the MNPs facilitated the convenient separation of MNPs from the bulks water samples. (hindawi.com)
Ionic liquids, which can be tuned by choosing of the cations and anions independently, exhibited unique physicochemical properties, such as tunable miscibility with water and organic solvents, and have potential applications as electrolyte materials, green solvents, catalysts, and separation mediums [ 9 - 14 ]. (hindawi.com)
N,N′-diallylimidazolium-based ionic liquids have also been used as extractant to highly enrich organic pollutants from aqueous samples in microextraction techniques, such as dispersed liquid-liquid microextraction and single drop microextraction, or to functionalize the stationary phase of chromatographic column and solid fiber membranes [ 15 - 21 ]. (hindawi.com)
The matrix is the basic material composed of the stationary phase, and the commonly used matrix materials are divided into three categories: inorganic, organic, and composite materials. (flashcolumns.com)
Selecting an organic modifier for reversed-phase chr. (chromatographytoday.com)
The organic analytes partition between the water sample and the stationary phase coating of a fused silica fiber before they are thermally desorbed in a GC injector. (cdc.gov)
Mobile phase (moving phase): organic solvents - Important properties: polarity. (profound-answers.com)
A low-viscosity mobile phase consisting of compressed trial organic matter in geosciences because the composition carbon dioxide is used in SFC to achieve fast and efficient of its phenols is an important indicator of the type of original separation. (lu.se)

Immunoaffinity chromatographic monoliths against RAE-1 GPI anchored glycoprotein were developed (CIMmic HDZ - @RAE-1 column) as a part of Glycomet project with the main goal to analyze the antigen glycoprofile. (biaseparations.com)

Therefore, in the HIC process, a solution with high salt concentration is used in the sample adsorption stage, so that the target molecules are bound in the chromatographic column, and in the elution stage, the hydrophobicity between the solute and the chromatographic medium is made by reducing the salt concentration in the eluent. (welch-us.com)
Mobile phases are of binary solvents (normally methanol and water) and the elution is done isocratically or with a linear gradient. (profound-answers.com)
Typically, a challenge for TLC separation is analyte co-elution (analytes not achieving adequate separation due to having similar retention times on the stationary phase). (microsaic.com)
Gradient elution was performed using toluene: methanol (10:0, 9.5:0.5, 9:1 up to 0:10) as the mobile phase. (jakenzyme.com)

capillary columns of different lengths for gas chromatographic analysis. (nighthelper.com)

The selectivity and separation efficiency are decided by the composition of the mobile and stationary phase and the column sizes. (amerigoscientific.com)
Mobile phase composition, for example, is the most powerful way of optimizing selectivity whereas temperature has a minor effect and would only achieve small selectivity changes. (pharmtech.com)

Compact prototype microfabricated gas chromatographic analyzer for autonomous determinations of VOC mixtures at typical workplace concentrations. (cdc.gov)
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. (lookformedical.com)

This improved economic performance is brought about by a valve-and-column arrangement that is used to lengthen the stationary phase indefinitely and allow very high solute loadings to the process. (wikipedia.org)
Rapid separation of the analyte from sample matrix was achieved in less than 60s using a Cromolith RP-18e monolithic column using UV detection at 220 nm. (shengsci.com)
This involves a sample being vaporised and injected onto the head chromatographic column. (majortests.com)
The sample is transported through the column by the flow of inert, gaseous mobile phase. (majortests.com)
The column itself contains a liquid stationary phase which is absorbed onto the surface of an inert solid. (majortests.com)
This technique allows you to use smaller particle size for the column packing material allowing a much better separation of the components of the mixture. (majortests.com)
The analytes are then removed from the trap by heating it and backflushing the analytes onto a gas chromatographic column. (cdc.gov)
The rate theory provides a more realistic explanation of the processes that take place inside a chromatographic column. (profound-answers.com)
The mobile phase is forced through an immiscible stationary phase which is fixed in place in a column or on a solid surface. (profound-answers.com)
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. (lookformedical.com)
A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. (lookformedical.com)
We tested several CEX columns under different conditions and the best column for isotype separation was weak cation-exchanging CIMac COOH chromatographic monolith in pH gradient. (biaseparations.com)
We apply the best-available software tools and our experience to help you select the best column and conditions for your separation needs. (mac-mod.com)
Minimum temperature is 50°C and maximum temperature is 250/260°C. This column is highly polar and custom designed for separation of Fatty Acid Methyl Esters (FAMEs). (utm.my)
It is industry standard column for FAME analysis and ideal for cis/trans isomer separation. (utm.my)
Minimum temperature is -20°C and maximum temperature is 280/300°C. This column is medium polar, highly inert and low bleed column. (utm.my)
High efficiency of our column and high resolution contribute to a nice peak separation. (chromservis.eu)
Packed column GC provides fast analysis times, allowing for quick and efficient separations. (nighthelper.com)
Packed column GC offers high-resolution separations, allowing for accurate identification and quantification of individual components in a sample. (nighthelper.com)
As technology continues to advance, packed column GC is expected to become even more widely used due to its enhanced sensitivity compared to other chromatographic techniques. (nighthelper.com)

Fig. 2: Mass chromatogram a real sample: Separation of the amino acids in a sample of Budvar beer 12˚ (25 µL of the sample was the initial load). (chromservis.eu)

Specifically, an SMB system has two or more identical columns, which are connected to the mobile phase pump, and each other, by a multi-port valve. (wikipedia.org)
All chromatographic methods -with the exception of TLC- use columns for the separation process. (ijpsr.com)
There are many different of kinds of chromatographic columns. (ijpsr.com)
During their work they had created columns packed with silica and water as a stationary phase and then used chloroform as a mobile phase to separate N-acetamino acids from protein hydrolysates. (cannabissciencetech.com)

What determines the movement of solute if the mobile phase is gas? (profound-answers.com)
Explanation: If the mobile phase is gas, movement of solute is determined by its volatility because gas is always measured as volatility not as solubility or melting point or boiling point. (profound-answers.com)
It takes into account the time taken by the solute to equilibrate between the stationary and mobile phase unlike the plate model which assumes that the equilibration is infinitely fast. (profound-answers.com)

A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. (lookformedical.com)

Development and validation of a liquid chromatographic method for the determination of ascorbic acid, dehydroascorbic acid and acetaminophen in pharmaceuticals. (shengsci.com)
A reversed-phase ion pair liquid chromatographic method (RP-LC) for the determination of dehydroascorbic acid (DHA) and ascorbic acid (AA) and also acetaminophen, which is combined in pharmaceuticals, is proposed and validated. (shengsci.com)
Dr. Michael Ye is the R&D Manager of Liquid Separation Department in Supelco, a division of Sigma-Aldrich. (ca-ca.org)
Can mobile phase be liquid? (profound-answers.com)
The mobile phase may be either a liquid or a gas, while the stationary phase is either a solid or a liquid. (profound-answers.com)
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. (lookformedical.com)
Chromatographic techniques in which the mobile phase is a liquid. (lookformedical.com)
A New Form of Chromatogram Employing Two Liquid Phases" in the Biochemical Journal in 1941 and was awarded a Nobel Prize in 1952. (cannabissciencetech.com)
Schomburg, G. Gas-and liquid chromatographic separation of naphthalenophanes of the [2.2] and [3.3] type. (mpg.de)
It has GCMS liquid sample injection, Headspace injection, Solid Phase Micro Extraction (SPME) and DI Probe. (utm.my)
In this technique, a phase-coated fused silica fiber is exposed to the headspace above the liquid or solid sample. (utm.my)

We hypothesize that pi-pi interactions between the aromatic carbonic acids and the ion-exchange resin are responsible for the strong retention on the stationary phase. (nih.gov)
The retention behavior was compared with that obtained on a commercially-available octadecylsilica (ODS) phase. (go.jp)
The results indicated a significantly larger retentivity of PACs on the PI stationary phase than that on typical ODS stationary phases, where the retention tendency on the PI phase for various PACs having different molecular size and shape was similar to the trend on the ODS phase. (go.jp)
The large retention on the PI phase can be interpreted on the basis of the surface structure of the PI particles, because a number of aromatic functionalities are in the chemical structure of the polymer chain backbone. (go.jp)

Schomburg, G. Preparative LC separation and isolation of enantiomerically pure olefins. (mpg.de)

Representation of octadecyl stationary phase morphology showing an ordered state and a disordered state. (nist.gov)

HIC media are composed of hydrophobic ligands such as alkyl or aromatic groups attached to a specific matrix such as agarose. (welch-us.com)
The RPC medium can be considered as a continuous hydrophobic phase, and the degree of substitution of ligands such as C4~C18 alkyl groups is usually in the hundreds of micromoles/mL gel: while the HIC medium has ligands such as C2~C alkyl groups or simple aromatic groups. (welch-us.com)

MStractor combines functions for molecular feature extraction with user-friendly dedicated GUIs for chromatographic and mass spectromerty (MS) parameter input, graphical quality-control outputs, and descriptive statistics. (bvsalud.org)
Molecular dynamics simulations of alkylsilane stationary-phase order and disorder. (nist.gov)

As analytes elute selectively through the stationary phase, separation is therefore achieved. (microsaic.com)
The properties of the mobile phase and the stationary phase are opposite of each other and allow for the partitioning of analytes as the mobile phase and test mixture flow through or over the stationary phase ( Figure 1b ). (cannabissciencetech.com)
Reverse phase is the choice for the majority of samples, but if acidic or basic analytes are present then reverse phase ion suppression (for weak acids or bases) or reverse phase ion pairing (for strong acids or bases) should be used. (pharmtech.com)

He has over 20 years experience in chromatographic analysis and separation including over 10 years experience in separation product research and development. (ca-ca.org)
The most variable aspect of analyses of this sort is the sample preparation procedure used to separate dibromochloromethane and bromoform from the test medium in order to prepare a sample suitable for GC analysis. (cdc.gov)
Dibromochloromethane and bromoform may be isolated from environmental samples using the same methods and principles as those used for biological materials, followed by gas chromatographic analysis. (cdc.gov)
Since only the volatiles are sampled, headspace analysis is ideal for dirty samples (e.g. blood, plastics, cosmetics), solid materials, samples with high boilers of no interest, samples with high water content, or samples that are difficult to handle by conventional chromatographic methods. (utm.my)
Unlike other carbohydrate analysis techniques, HPAE-PAD allows for highly selective separation and quantification of non-derivatized carbohydrates. (thermofisher.com)
Learn the basics of HPAE-PAD, the optimal chromatographic technique for carbohydrate analysis. (thermofisher.com)

Ethanol was successfully used in RP-LC as an environmentally friendly (green) chromatographic method with particulate stationary phases [17-20], but often with small flow rates. (drinksavvyinc.com)
For Solid Phase Micro Extraction (SPME), it is an equilibrium sampling method and can be used for gases, solids (Headspace) and liquids (direct). (utm.my)

The interaction between HIC media and hydrophobic biomolecules is believed to be driven by entropy increase and change in free energy, just like the spontaneous aggregation of hydrophobic molecules in aqueous systems. (welch-us.com)

Separation technique in which the stationary phase consists of ion exchange resins. (lookformedical.com)

What is mobile phase composition? (profound-answers.com)

The chromatographic conditions included ascending separation technique, twin trough chamber for plate development, chamber saturation time 4 min and migration distance 10 cm at a temperature of 25 �� 2��C. Detection was done in UV�Cvisible range. (jakenzyme.com)
Over the subsequent decades this procedure was expanded as a separation technique by the Nobel Prize winning work of Archer J.P. Martin and Richard L.M. Synge in the 1940s and 1950s. (cannabissciencetech.com)

This resulted in a good separation from interfering substances after direct injection of urine. (nih.gov)

8 ug injected mass, linear calibration curves, no humidity effects, excellent medium-term (that is, 1 week) reproducibility, autonomous operation for 8 h, detection limits below Threshold Limit Values (TLV) for 10 mL air samples collected in 1 min, and response patterns that enhanced vapor recognition. (cdc.gov)

His interests includes developing new chromatographic stationary phases and application methods for bio-separation and small molecules. (ca-ca.org)
Examples include colloidal particle stabilization, chromatographic materials, and drug delivery methods. (nist.gov)

Cyano-bonded phases are easier to work with than plain silica for normal phase separations. (pharmtech.com)

Thus, it is well-suited for separation of a binary mixture. (wikipedia.org)

Generally speaking, hydrophilic small molecules are difficult to interact with HIC media. (welch-us.com)

Mobile phase shouId be inert towards sample constituents and the stationary phase. (profound-answers.com)
How is a sample dissolved in a mobile phase? (profound-answers.com)
Sample spots (which can be quickly prepared from complex matrices) are applied to a stationary phase. (microsaic.com)
To see examples of how the MiD and TLC-MS 2 can work together to give information rich data from complex sample media please refer to our Application Notes section. (microsaic.com)
Pipette 50 μL of extraction medium into each sample preparation vial and vortex 5 to 10 sec. (chromservis.eu)

Bien-Vogelsang, U. Immobilization of stationary liquids on silica particles by γ-radiation. (mpg.de)

For chromatographic media with aromatic groups as hydrophobic ligands, there is also a potential possibility of π-π interaction. (welch-us.com)
When the surface of the substance to be separated has aromatic groups, it will show a mixed separation of hydrophobic interaction and π-π interaction. (welch-us.com)
Thereby, we could show that the OMM 12 interaction network is shaped by both exploitative and interference competition in vitro in nutrient-rich culture media and demonstrate how community structure can be shifted by changing the nutritional environment. (nature.com)

The mobile phase flows through the paper, carrying the components of the mixture with it. (profound-answers.com)
Dilute derivatization reaction mixture with 400 μL of diluting and washing medium and vortex 5-10 sec. (chromservis.eu)

Deficiency of medium-chain acyl-CoA dehydrogenase is a frequent and treatable metabolic defect, which can be diagnosed by detection of phenylpropionylglycine in urine after an oral load of phenylpropionic acid. (nih.gov)

Developed by advanced patented technology, our media have high resolution and long lifetime, and can be used in biological, pharmaceutical, chemical and other fields. (amerigoscientific.com)

Chromatographic separation techniques now published in Ph. (edqm.eu)
We have investigated the conformational phase behavior of tethered chain systems using temperature expanded Monte Carlo techniques at thousands of state points, covering densities from the isolated chains to the thin film limit, and a broad range of temperatures. (nist.gov)

Capillaries offer much better efficiency (narrow peaks) which leads to greatly better-quality peak separation. (utm.my)

Anatomy8

Organisms23

Diseases1

Chemicals and Drugs99

Analytical, Diagnostic and Therapeutic Techniques and Equipment64

Psychiatry and Psychology2

Phenomena and Processes56

Disciplines and Occupations2

Technology, Industry, Agriculture4

Information Science7

Health Care9

Chromatography64

HPLC9

Solvent13

Compounds8

Chiral stationary2

Analytical2

Silica gel5

Affinity2

Characterization2

Purification10

Organic8

Monoliths1

Elution4

Capillary Columns1

Selectivity2

Mixtures2

Column20

Amino1

Columns4

Solute3

Utilizes1

Liquid11

Retention4

Isolation1

Octadecyl1

Alkyl2

Molecular2

Analytes3

Analysis6

Method2

Aqueous1

Resins1

Composition1

Technique2

Substances1

Mass1

Methods2

Normal phase1

Binary1

Small molecules1

Sample5

Particles1

Interaction3

Mixture2

Detection1

Chemical1

Techniques2

Efficiency1