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  • proteins
  • DNA methylation is a robust biomarker, vastly more stable than RNA or proteins, and is therefore a promising target for the development of new approaches for diagnosis and prognosis of breast cancer and other diseases. (biomedcentral.com)
  • arginine
  • The three types of PRMTs share the ability to mono-methylate arginine residues, but vary in their ability to generate differential methylation states (1-3). (cellsignal.com)
  • Research studies indicate that PRMT7 is a type III PRMT that displays substrate specificity for an arginine-X-arginine (RXR) motif surrounded by several basic residues (4,5). (cellsignal.com)
  • DNMT1
  • DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. (cellsignal.com)
  • Epigenetics
  • Using RNA epigenetics and chromatin structures as diagnostic tools can help predict and reduce drug resistance in treating patients with leukemia. (medindia.net)
  • structures
  • The genome is organized into particular chromatin structures that have specific roles both in maintaining the overall structure and in gene expression. (hindawi.com)
  • In human cells, chromatin is organized into distinct structures with functional domains that regulate gene expression and stem cell development. (medindia.net)
  • They show that 5-AZA-sensitive and 5-AZA-resistant leukemia cells have distinctly different chromatin structures that are associated with RNA:m5C and RCMTs at nascent RNA, as schematically illustrated. (medindia.net)
  • sequential
  • The sequential treatment of PMF CD34 + cells with the chromatin-modifying agents 5-aza-2′-deoxycytidine (5azaD) and trichostatin A (TSA), but not treatment with small molecule inhibitors of JAK2, resulted in the generation of increased numbers of CD34 + CXCR4 + cells, which was accompanied by enhanced homing of PMF CD34 + cells to the marrow but not the spleens of NOD/SCID mice. (aacrjournals.org)
  • Our data suggest that PMF CD34 + cells are characterized by a reduced ability to home to the marrow but not the spleens of NOD/SCID mice and that this homing defect can be corrected by sequential treatment with chromatin-modifying agents. (aacrjournals.org)
  • clinical
  • However, because DNAm analysis is mainly performed by DNA methylation array systems, the versatility of DNAm measurement is low in clinical use. (hindawi.com)
  • By applying new technologies--including mass spectrometric analysis and proximity ligation rolling circle amplification confocal microscopy--to a small size of clinical MDS/AML specimens (n=18), the researchers demonstrated a significant increase in RNA:m5C and RCMTs and the NSUN1/BRD4-associated chromatin structure in 5-AZA-resistant vs. 5-AZA-sensitive clinical MDS/AML specimens. (medindia.net)
  • Drugs that modulate DNA methylation are in clinical trials ( 5 ) and have been shown to affect gene expression in vivo ( 6 ). (aacrjournals.org)
  • assay
  • The MethyLight assay is a methylation-specific PCR-based technique, which is a quick and easy method for measuring DNAm, and has the potential to readily screen large numbers of samples [ 14 ]. (hindawi.com)
  • By using an in vivo one-hybrid DNA-binding assay in cultured human cells we found that CpG methylation had no appreciable effect on the affinity of SB transposase to its binding sites. (biomedcentral.com)
  • biomarkers
  • The methylation rates of cg23576855 and cg05573951 in AHRR , cg03636183 in F2RL3 , and cg21566642 in ALPPL2 are well known biomarkers for cigarette smoking [ 7 , 8 ], and more than thirty CpG sites were reported as significantly associated probes in short-term alcohol dependence treatment programs [ 9 ]. (hindawi.com)
  • The relative contribution of DNA methylation and genetic variants on protein biomarkers for human diseases. (diva-portal.org)
  • In this study, we combined SNP and DNA methylation data with measurements of protein biomarkers for cancer, inflammation or cardiovascular disease, to investigate the relative contribution of genetic and epigenetic variation on biomarker levels. (diva-portal.org)
  • A total of 121 protein biomarkers were measured and analyzed in relation to DNA methylation at 470,000 genomic positions and to over 10 million SNPs. (diva-portal.org)
  • Study
  • The aim of this study was to determine how DNA methylation is changed after arsenic-induced malignant transformation. (omicsdi.org)