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  • vesicle
  • Wortmannin and BDM affect drastically vesicle transport throughout the cell cytoplasm, including the region beneath the plasma membrane. (biochemj.org)
  • O nce attached at the cell membrane (CM), 1 a secretory vesicle may acquire the capability to immediately release upon stimulation. (rupress.org)
  • First, we showed that it is possible to extract information on vesicle motion in Chromaffin cells from electrophysiological experiments. (uni-kassel.de)
  • In particular, we performed Langevin simulations of vesicle motion towards the cell membrane assuming pure Brownian motion and obtained the statistics of vesicle arrival times. (uni-kassel.de)
  • We realized that, in the steady state, each amperometric peak can be related to a single release from a new vesicle arriving at the active site of the cell membrane and that the amperometric signal can be mapped into a time-sequence of release events. (uni-kassel.de)
  • secrete
  • Adrenal chromaffin cells (ACCs) secrete several neuroactive substances that are effective in influencing pain sensitivity in the central nervous system as well as enhancing the recovery of the intrinsic nigrostriatal dopaminergic system in patients with Parkinson's disease. (biomedsearch.com)
  • Closely related to the nervous system, chromaffin cells are one of the most commonly studied neural crest derivatives due to the variety of bioactive substances they secrete into the bloodstream, including adrenaline, noradrenaline, and other hormones. (altogen.com)
  • Adrenal medulla, the central region of these glands, contains the cells that synthesize and secrete these hormones. (altogen.com)
  • Intracellular
  • one due to influx from the cell exterior through voltage-gated Ca 2+ channels, and that due to release from intracellular stores. (rupress.org)
  • cultures
  • b , d Averaged pooled data (mean ± SEM) of the number of experiments shown in parentheses (number of cells and cultures). (springer.com)
  • Data are means ± SEM of the number of cells and cultures given in parentheses. (springer.com)
  • Primary cultures of bovine adrenal chromaffin cells provide large quantities of a homogeneous population of target cells for nerve growth factor (NGF) and, thus, are a suitable system for studying the molecular mechanism of action of NGF. (jneurosci.org)
  • Primary cultures of chromaffin cells from bovine adrenal medulla were used as a model to evaluate the ability of 8-Br cyclic AMP (8-Br cAMP) to induce tyrosine hydroxylase (TH) and to study the role of cAMP-dependent protein kinase (cAPK) in this induction. (aspetjournals.org)
  • ACCs
  • By the use of 3-D PLGA as support for ACCs, it is possible to upregulate metabolic function and localize a high number of morphologically healthy-looking cells. (biomedsearch.com)
  • Highly purified ACCs cultured on PLGA scaffold may have promise in transplantation studies, because these cells are less immunogenic and may be applied to in vivo settings by using short-term immunosuppression. (biomedsearch.com)
  • nicotinic
  • Two probes previously shown to distinguish the nicotinic ACh receptor of chick ciliary ganglion neurons also recognize a component on the surface of bovine chromaffin cells in culture that displays the properties expected for the chromaffin nicotinic ACh receptor. (jneurosci.org)
  • These results indicate that the bovine chromaffin component recognized by mAb 35 and Bgt 3.1 is likely to be the nicotinic ACh receptor on the cells and that it has many similarities to ACh receptors on chick autonomic neurons. (jneurosci.org)
  • Nicotinic Cholinergic Regulation of Tyrosine Hydroxylase Gene Expression and Catecholamine Synthesis in Isolated Bovine Adrenal Chromaffin Cells. (go.jp)
  • By quantitative confocal immunofluorescence, immunoreplica analysis and immunoprecipitation, we show here the translocation of p36 from the cytosol to a subplasmalemmal Triton X-100 insoluble fraction in chromaffin cells following nicotinic stimulation. (naver.com)
  • stimulation
  • Upon depolarization of cells expressing fluorescent Syt isoforms using elevated K + , we find that Syt-7 granules fuse with faster kinetics than Syt-1 granules, irrespective of stimulation strength. (rupress.org)
  • They found that strong stimulation of the cells leads to depletion of the pool of RRGs, and that slight elevation of [Ca 2+ ] leads to an acceleration in refilling the pool of RRGs. (rupress.org)
  • protein
  • Protein expression of Cyclophilin B in Chromaffin cells. (altogen.com)
  • At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). (altogen.com)
  • Within cells, annexin II is found either as a 36-kD monomer (p36) or as a heterotetrameric complex (p90) coupled with the S-100-related protein, p11. (naver.com)
  • The light chain of annexin II, p11, was selectively expressed by adrenergic chromaffin cells, and was only present in the subplasmalemmal Triton X-100 insoluble protein fraction of both resting and stimulated cells. (naver.com)
  • Thus, our strategy was based in overexpressing native as well as altered forms of SNAP-25 coupled to green fluorescent protein (GFP) in bovine chromaffin cells and studying the effect produced by these constructs in the secretory properties of the cells. (pnas.org)
  • vitro
  • In vivo and in vitro phosphorylation of annexin II in T cells: potential regulation by annexin V. (naver.com)
  • cytosolic
  • such effects of H 2 S donors are aborted by cyclopiazonic acid (CPA), that causes endoplasmic reticulum (ER) Ca 2+ depletion through sarcoendoplasmic reticulum Ca2+ ATPase inhibition and by protonophore carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP), that impedes the ability of mitochondria to sequester cytosolic Ca 2+ during cell depolarization. (springer.com)
  • predominantly
  • Immunohistochemistry showed that approximately 70% of chromaffin cells had phospho-STAT3 within their nuclei the distribution of phospho-ERK1/2 was predominantly within the cytoplasm of these cells. (otago.ac.nz)
  • experiments
  • Encouraged by the successful analysis on Chromaffin cells we decided to study and simulate miniature excitatory postsynaptic currents (mEPSC) and miniature excitatory junction potentials (mEJP), based on the results of patch-clamp experiments performed by collaboration partners at the University of Heidelberg (Dr. Peter Bengtson and Prof. Dr. Christoph Schuster). (uni-kassel.de)