• [1] During embryonic development of birds, the chorioallantoic membrane also plays an essential role in bone formation by transporting calcium into the embryo from the eggshell. (wikipedia.org)
  • The Chorioallantoic membrane performs the following functions: The CAM functions as the site of gaseous exchange for oxygen and carbon dioxide between the growing embryo and the environment. (wikipedia.org)
  • There are also problems associated with the handling of the embryo, as the yolk membrane is prone to rupture both during and after the culture. (wikipedia.org)
  • In this method, fertilized eggs are rotated inside an incubator for three days in order to prevent the embryo from sticking to the membranes of the shell. (wikipedia.org)
  • The authors studied the specific tumor antigen in Crocker's sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the developing chick embryo. (springer.com)
  • Other models, such as the chick embryo chorioallantoic membrane (CAM) assay, are therefore an attractive alternative. (mdpi.com)
  • The chicken embryo and the blood-vessel rich chorioallantoic membrane (CAM) is a valuable in vivo model to investigate biomedical processes, new ultrasound pulsing schemes, or novel transducers for contrast-enhanced ultrasound imaging and microbubble-mediated drug delivery. (jove.com)
  • The protocol also describes how to inject cavitation nuclei, such as microbubbles, into the CAM vascular system, how to separate the membrane containing the embryo and CAM from the rest of the egg content for optically transparent studies, and how to use the chicken embryo and CAM in a variety of short-term ultrasound experiments. (jove.com)
  • When a suspension of living adult chick leucocytes is placed in contact with the chorioallantoic membrane (CAM) of a developing chick embryo, a variable number of white focal lesions appear a few days later. (royalsocietypublishing.org)
  • We hypothesized that the chorioallantoic membrane (CAM) of the chicken embryo is a more suitable culture system than traditional in vitro culture. (usask.ca)
  • After warming, the vitrified tissues were either immediately placed in 10% formalin (control) or on the chorioallantoic membrane of a 10-day old chicken embryo for 5 days. (usask.ca)
  • The chick embryo chorioallantoic membrane (CAM) is commonly used in vivo to study both angiogenesis and anti-angiogenesis. (elsevier.com)
  • Objective: In this study we have compared in an in vivo assay, the chick embryo chorioallantoic membrane, the angiogenic potential of mast cell suspensions isolated from rats, degranulated mast cells and their secretory granules. (elsevier.com)
  • Furthermore, DHT-treated, PTX3 overexpressing hPTX3_S115 cell transfectants show a reduced proliferation rate in vitro and a limited angiogenic activity in the chick embryo chorioallantoic membrane and murine s.c. (aacrjournals.org)
  • Inhibition of the mentioned essential steps of in vitro angiogenesis is in agreement with the observed antiangiogenic activity, substantiated by using two in vivo angiogenesis models, the chorioallantoic membrane and the zebrafish embryo neovascularization assays, and by the ex vivo mouse aortic ring assay. (mdpi.com)
  • The specific tissue of origin for these enzymes remains to be elucidated, but metabolic enzymes are expressed in the chorioallantoic membrane [ 18 ] and embryo [ 19 ] of the chicken. (royalsocietypublishing.org)
  • This core facility provides scientific expertise and services using the chick embryo model specifically, its chorioallantoic membrane (CAM). (ipatimup.pt)
  • To assess the antiangiogenic effect of aliskiren, a direct renin inhibitor, on chick embryo chorioallantoic membrane (CAM) assay. (conicyt.cl)
  • The main function of the Catecholamines (CA): noradrenaline-NA, adrenaline-A and dopamine-DA is to protect the embryo from deleterious hypoxic damages in the end of embryogenesis (Hypoxie) by improving the blood gas status and the redistribution of cardiac output in favour of heart, brain and chorioallantoic membrane. (fu-berlin.de)
  • We have previously reported that individual neonatal foreskin stromal cells (hNSSCs) promote angiogenesis in vitro and in girl embryo chorioallantoic membrane layer (Camera) assay in vivo. (healthandwellnesssource.org)
  • Keywords: Individual neonatal foreskin stromal cells (hNSSCs), Girl embryo chorioallantoic membrane layer (Camera), Individual umbilical line of thinking endothelial cells (HUVEC), Micro mass, Girl femur, Ex girlfriend vivo, Organotypic lifestyle 1. (healthandwellnesssource.org)
  • An attractive alternative is the chick chorioallantoic membrane (CAM) assay. (mdpi.com)
  • Using a chick chorioallantoic membrane (CAM) implanted with xenografts harvested from C57BL/6 mice with B16F10 melanoma cells, we studied the effects of iron oxide nanoparticles functionalized with salicylic acid (SaMNPs) as a form of therapy on the local development of xenotransplants and CAM vessels. (mdpi.com)
  • Gels containing melanoma cells were grafted onto chick chorioallantoic membranes (CAMs). (arvojournals.org)
  • Anti-angiogenic activity was tested in chick chorioallantoic membrane (CAM) assays (staurosporine positive control). (arvojournals.org)
  • Chorioallantoic membrane (CAM) assay was carried out as previously described ( 18 ). (aacrjournals.org)
  • [2] As a result, the chorioallantoic membrane allows exchange of gases, such as oxygen , to developing embryos . (wikipedia.org)
  • Ex ovo chicken embryos and the blood-vessel rich chorioallantoic membrane (CAM) have proven to be a suitable model to investigate various biological and biomedical processes like embryogenesis, oncology, and drug delivery 1 , 2 , 3 , 4 . (jove.com)
  • Results: Isolated mast cells and their secretory granules, but not degranulated mast cells, induced an angiogenic response in the chorioallantoic membrane. (elsevier.com)
  • Methods: Gelatin sponges adsorbed with cell suspensions of rat mast cells, degranulated mast cells and their secretory granules were implanted on the top of the chorioallantoic membrane at day 8 of incubation. (elsevier.com)
  • Chicken chorio allantoic membrane (CAM) on 9th day of incubation was incubated with 1, 3 and 5 μg concentration of HRL for 72 h using gelatin sponge. (biomedcentral.com)
  • Gelatin sponges soaked with leptin were placed on the membrane at 9th day of post incubation so as to allow slow delivery of the chemical at the treated area with less or no inflammation [ 13 , 14 ]. (biomedcentral.com)
  • The first objective was to compare the health and development of preantral follicles in bovine ovarian tissue, as well as the neovascularization of these tissues, subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. (usask.ca)
  • Rapid membrane water transport is mediated by a family of molecular water channels, called aquaporins (AQPs), which have been identified in the epithelial and endothelial cells of higher vertebrates. (elsevier.com)
  • Sprouting is a multistep process involving the dissolution of the basement membrane, the migration and proliferation of endothelial cells, the formation of a lumen and maturation of the new vessel with investment of the vessel with pericytes [ 3 ]. (mdpi.com)
  • The chorioallantoic membrane - also called the chorioallantois or abbreviated to CAM - is a vascular membrane found in eggs of some amniotes , such as birds and reptiles . (wikipedia.org)
  • The Chorioallantoic Membrane (CAM), also known as the chorioallantois, is a highly vascularized membrane found in the eggs of certain amniotes like birds and reptiles. (wikipedia.org)
  • Membrane most commonly means a thin, selective barrier, but it is sometimes used for films that function as separators, like biological membranes. (wikipedia.org)
  • CAM provides several features such as ease of access, and the rapid development of the membrane structure, presence of an immunodeficient environment, ease of visualization for imaging techniques ranging from microscopic to PET scans. (wikipedia.org)
  • Blood capillaries and sinuses are found between epithelial cells of the chorionic layer, allowing close contact (within 0.2 μm) with air found in pores of the shell membrane of the egg. (wikipedia.org)
  • The first is the chorionic epithelium that is the external layer present immediately below the shell membrane. (wikipedia.org)
  • Results: Isolated mast cells and their secretory granules, but not degranulated mast cells, induced an angiogenic response in the chorioallantoic membrane. (elsevier.com)
  • Furthermore, DHT-treated, PTX3 overexpressing hPTX3_S115 cell transfectants show a reduced proliferation rate in vitro and a limited angiogenic activity in the chick embryo chorioallantoic membrane and murine s.c. (aacrjournals.org)
  • Angiogenic potential of Aleurites moluccana (L.) Wild (Lumbang) crude bark and seed extracts on the chorioallantoic membrane (CAM) of a 10-day old chic embryo. (edu.ph)
  • Angiogenic of Broussonettia luzonica (Himbabao) and Caesalpinia pulcherrima (Caballero) crude flower extracts on the chorioallantoic membrane (CAM) of a 10-day old chick embryo. (edu.ph)
  • Specifically, we investigated the ability of integrin αvβ3 and bFGF to influence MAP kinase activity in angiogenic blood vessels within the chick chorioallantoic membrane (CAM). (rupress.org)
  • Pathogenicity of Nocardia for chorio-allantoic membrane of the chick embryo. (bvsalud.org)
  • Chicken chorio allantoic membrane (CAM) on 9th day of incubation was incubated with 1, 3 and 5 μg concentration of HRL for 72 h using gelatin sponge. (biomedcentral.com)
  • The chick embryo chorioallantoic membrane (CAM) model is well described in human medicine as a cost-effective, easy to perform preclinical oncological model for observing pro- and antiangiogenic response, tumor biology and metastasis. (biomedcentral.com)
  • Chick embryo chorioallantoic membrane model systems to study and visualize human tumor cell metastasis. (mysciencework.com)
  • The ability of the chick embryo's chorioallantoic membrane (CAM) to efficiently support the growth of inoculated xenogenic tumor cells greatly facilitates analysis of human tumor cell metastasis. (mysciencework.com)
  • The download The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis: The Web and the crisis tools differed an mistake that would navigate monopoly in the p., a method that refers up the interaction and the day. (itsys-rw.de)
  • The applications produced Invented for download The Chick Embryo Chorioallantoic Membrane in the Study of Angiogenesis and Metastasis: The CAM or Comment operations at days, production fields and countries. (itsys-rw.de)
  • The first objective was to compare the health and development of preantral follicles in bovine ovarian tissue, as well as the neovascularization of these tissues, subjected to avian chorioallantoic membrane (CAM) culture with the traditional in vitro culture system. (usask.ca)
  • Upon initiation of angiogenesis with basic fibroblast growth factor (bFGF) on the chick chorioallantoic membrane (CAM), endothelial cell mitogen-activated protein (MAP) kinase (ERK) activity was detected as early as 5 min yet was sustained for at least 20 h. (rupress.org)
  • Here we describe the use of the chicken chorioallantoic membrane (CAM) tumor model in studies on oncolytic adenoviral vectors. (nih.gov)
  • The chicken embryo and the blood-vessel rich chorioallantoic membrane (CAM) is a valuable in vivo model to investigate biomedical processes, new ultrasound pulsing schemes, or novel transducers for contrast-enhanced ultrasound imaging and microbubble-mediated drug delivery. (jove.com)
  • Chick embryo chorioallantoic membrane model systems to study a. (mysciencework.com)
  • Since their introduction almost a century ago, chick embryo model systems involving the technique of chorioallantoic grafting have proved invaluable in the in vivo studies of tumor development and angiogenesis and tumor cell dissemination. (mysciencework.com)
  • In this thesis the chicken chorioallantoic membrane (CAM) model was used to study the enhanced delivery of intravenously injected \SI{2}{\mega\dalton} FITC-dextrans using ultrasound in combination with microbubbles. (ntnu.no)
  • Chicken chorioallantoic membrane (CAM) models have been applied as an affordable and efficient angiogenesis model for many years in relevant areas such as cancer angiogenesis and cardiovascular sciences. (vascularcell.com)
  • In the present work, the effects of three AChE inhibitors donepezil, rivastigmine, and galantamine were tested on H2O2-induced apoptosis in human umbilical vein endothelial cells HUVECs and on angiogenesis in chicken chorioallantoic membrane model. (duhnnae.com)
  • As such, the purpose of this study was to investigate the antiangiogenic effects of Amiodarone HCl in the chorioallantoic membrane model. (cumhuriyet.edu.tr)
  • JNJ-26076713 blocks cell migration induced by vascular endothelial growth factor, fibroblast growth factor (FGF), and serum, and angiogenesis induced by FGF in the chick chorioallantoic membrane model. (aspetjournals.org)
  • Optimising the chick chorioallantoic membrane xenograft model of neuroblastoma for drug delivery. (pubfacts.com)
  • Features of the model chorioallantoic membrane. (ebscohost.com)
  • Objective: The aim was to compare the irritation potency of frequently used wound antiseptics by using the hen's egg test on the chorioallantoic membrane (HET-CAM). (uni-greifswald.de)
  • Effect of ethyl acetate (EtOAc) fraction of V. tricolor on the number and diameter of vessels in chorioallantoic membrane (CAM). (hindawi.com)
  • For the purpose of determining the antiangiogenic effects of the drugs, blood vessels of the chorioallantoic membranes were evaluated using a stereoscopic microscope. (cumhuriyet.edu.tr)
  • Blood capillaries and sinuses are found between epithelial cells of the chorionic layer, allowing close contact (within 0.2 μm) with air found in pores of the shell membrane of the egg. (wikipedia.org)
  • It is the outermost extra-embryonic membrane which lines the non-vascular egg shell membrane. (wikipedia.org)
  • The first is the chorionic epithelium that is the external layer present immediately below the shell membrane. (wikipedia.org)
  • This step prevents the CAM from sticking to the shell membrane. (wikipedia.org)
  • At day 14, the capillary plexus is located at the surface of the ectoderm adjacent to the shell membrane. (alpfmedical.info)
  • The beak is against the inner shell membrane, ready to pierce it. (thepoultrysite.com)
  • The chick pierces the inner shell membrane and breathes in the air cell. (thepoultrysite.com)
  • Role of FGF-2 in chorioallantoic membrane vascularization 4. (moluna.de)
  • When a suspension of living adult chick leucocytes is placed in contact with the chorioallantoic membrane (CAM) of a developing chick embryo, a variable number of white focal lesions appear a few days later. (royalsocietypublishing.org)
  • To investigate the inhibitory effect of lentiviral vector-mediated short hairpin RNA targeting survivin (LV-survivin shRNA) on angiogenesis and growth of endometriosis-like lesions in chick en embryo chorioallantoic membrane. (bvsalud.org)
  • By days 12-15, the first visible lesions - small reddish spots called enanthem - appeared on mucous membranes of the mouth, tongue, palate , and throat, and temperature fell to near-normal. (wikipedia.org)
  • Fifteen Influenza A virus reference subtypes, H1 to H15, infected chicken embryonated chorioallantoic membranes (CAM) fixed by formalin, were all detectable in WF-4 based IHC. (flutrackers.com)
  • After warming, the vitrified tissues were either immediately placed in 10% formalin (control) or on the chorioallantoic membrane of a 10-day old chicken embryo for 5 days. (usask.ca)
  • To define the local influence of intravascular flow fields on vessel structure, we have studied 2nd and 3rd order extra-embryonic microvessels in the chick chorioallantoic membrane (CAM). (biomedcentral.com)
  • Under normal conditions the chorioallantoic membrane (CAM) and developing avian kidney work in unison to ensure a proper balance of ions and water within the egg. (unt.edu)
  • The authors studied the specific tumor antigen in Crocker's sarcoma of mice and M-1 sarcoma of rats as well as in heterotransplants of these tumors on the chorioallantoic membrane of the developing chick embryo. (springer.com)
  • Here, we studied the use of the chick chorioallantoic membrane (CAM) for the generation of human BL xenograft tumors, which we compared with known characteristics of the human disease. (beds.ac.uk)
  • For example, MMP2 and its activator membrane type 1 (MT1)-MMP are coordinately expressed by mesenchymal cells almost exclusively during embryonic development, indicating specific matrix remodeling constraints in these tissues ( 1 ). (pnas.org)
  • It is also associated with preterm birth and has been isolated from the amniotic fluid, placenta, and chorioamnionic membranes of women delivering prematurely. (asm.org)
  • We hypothesized that the chorioallantoic membrane (CAM) of the chicken embryo is a more suitable culture system than traditional in vitro culture. (usask.ca)
  • The earliest microbicide candidates were surfactants, such as nonoxynol-9 (N9), that showed antiretroviral activity against both HIV and HSV in vitro by disrupting the membrane coat of the virus 69-71]. (thefreedictionary.com)
  • Instead, the membrane-anchored MMP, MT1-MMP, confers endothelial cells with the ability to express invasive and tubulogenic activity in a collagen-rich milieu, in vitro or in vivo, where it plays an indispensable role in driving neovessel formation. (rupress.org)
  • There are also problems associated with the handling of the embryo, as the yolk membrane is prone to rupture both during and after the culture. (wikipedia.org)
  • The vitelline membrane spreads over the yolk surface. (thepoultrysite.com)
  • The vitelline membrane continues to grow and now surrounds more than half the yolk. (thepoultrysite.com)
  • The vitelline membrane covers almost the whole yolk. (thepoultrysite.com)
  • The vitelline membrane completely surrounds the yolk. (thepoultrysite.com)
  • abstract = "The chick embryo chorioallantoic membrane (CAM) is commonly used in vivo to study both angiogenesis and anti-angiogenesis. (elsevier.com)
  • Invasion of the membrane led to death of the embryo due to damage of the CAM, which could be examined histologically to show cell distribution and morphology, and by RT-PCR for assessment of patterns of fungal gene expression in vivo. (elsevier.com)
  • represents an experimentally tractable and inexpensive alternative to rodent or tissue culture-based invasion models, and can be used to investigate fungal pathogenesis and the genetic regulation of infection and membrane penetration of C albicans. (elsevier.com)
  • The protocol also describes how to inject cavitation nuclei, such as microbubbles, into the CAM vascular system, how to separate the membrane containing the embryo and CAM from the rest of the egg content for optically transparent studies, and how to use the chicken embryo and CAM in a variety of short-term ultrasound experiments. (jove.com)
  • Extracellular matrix-degradative enzymes, including the matrix metalloproteinases (MMPs) MMP-2 and MMP-9, are thought to play key roles in angiogenesis by binding to docking sites on the cell surface after activation by plasmin- and/or membrane-type (MT) 1-MMP-dependent processes. (rupress.org)
  • [1] During embryonic development of birds, the chorioallantoic membrane also plays an essential role in bone formation by transporting calcium into the embryo from the eggshell. (wikipedia.org)
  • 1985) Electron download The Chick Embryo Chorioallantoic etc. of return subscription scores in the Jet Rock Formation( Lower Toarcian) of the Whitby accretion, UK. (itsys-rw.de)
  • Furthermore, possible side effects such as membrane irritation, toxic, and anticoagulant properties of the investigated material in question can be detected. (uni-regensburg.de)
  • Conclusions: The chorioallantoic membrane culture system can be used to sustain the growth and development of isolated chick and quail lung buds, thus permitting their experimental manipulation to determine the role of different cell populations in lung development. (caltech.edu)
  • Lentiviral delivery of PEX blocked basic fibroblast growth factor-induced matrix metalloproteinase 2 activation and angiogenesis on chicken chorioallantoic membranes. (pnas.org)
  • The chorioallantoic membrane vascular growth 3. (moluna.de)
  • Fluorescently labeled murine mammary carcinoma cells, isolated from either MMTV-PyVmT transforming growth factor-beta receptor II knockout (TβRII KO) or TβRII fl/fl control mice, were combined with mammary fibroblasts and xenografted onto the chicken embryo chorioallantoic membrane. (biomedcentral.com)