Chondrogenic differentiation cells that form cartilage cartilage chondrosarcoma mesenchymal. Medical search. Frequent questions

They are located on the perichondrium because the perichondrium, located on the outside of developing bone, is not as heavily ensheathed in cartilage extracellular matrix as the interior and because here where capillaries are located. (wikipedia.org)
Expression of this gene will also prompt already formed cartilage to undergo endochondral ossification which will prompt the cartilage to form bone. (wikipedia.org)
Bone sarcomas such as osteosarcoma and chondrosarcoma are frequently refractory to conventional chemotherapy and radiotherapy that exhibit poor prognosis. (oncotarget.com)
Here we demonstrate aberrant activation of Wnt/β-catenin signaling in bone sarcoma cells, involving an autocrine Wnt signaling loop with upregulation of specific Wnt ligands and receptors. (oncotarget.com)
Activation of Wnt/β-catenin signaling with Wnt3a or GSK-3β inhibitor drives the proliferation of bone sarcoma cells, whereas downregulation of activated Wnt signaling with dnTCF4 or siLEF1 suppresses bone sarcoma proliferation and induces cell cycle arrest. (oncotarget.com)
Taken together, our findings establish the evidence that aberrant activation of Wnt/β-catenin pathway involving an autocrine Wnt singaling drives the proliferation of bone sarcoma cells, and identify the autocrine activation of the Wnt/β-catenin signaling as a potential novel therapeutic target for bone sarcomas. (oncotarget.com)
Osteosarcomas and chondrosarcomas are recognized as the most common primary bone sarcomas [ 1 ], which involve mesenchymal tissues and exhibit highly heterogeneous histologic and molecular profiles [ 2 ]. (oncotarget.com)
Here, we report two challenging cases involving 17- and 9-years-olds males where diffuse and multiplex bone lesions suggested either a hematological disease or a primary bone tumor (mesenchymal chondrosarcoma). (biomedcentral.com)
Morscheid S., Rey-Rico A., Schmitt G., Madry H., Cucchiarini M., Venkatesan J.K. Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-β and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells. (uniklinikum-saarland.de)
Dedifferentiated chondrosarcoma is an aggressive mesenchymal tumor of the bone, and novel therapies are needed to improve its clinical outcomes. (cancer-genetics.org)
The pathologic changes in OA joints include degradation of the articular cartilage, especially at the superficial zones, thickening of the subchondral bone, osteophyte formation, and variable degrees of synovial inflammation 11 . (nature.com)
A typical distinction of KBD from OA or other bone and joint diseases is the damage of epiphyseal cartilage and hyaline cartilage in the deep zones of cartilage 11 , 14 . (nature.com)
Bone morphogenic protein and transforming development component B signaling is one of the crucial pathways controlling chondrogenic differentiation while in the ordinary growth plate. (sirnalibrary.com)
The primary paracrine elements in the TGFB superfamily appropriate for cartilage and bone formation are BMP2, BMP4, BMP6, BMP7, TGFB1, TGFB2 and TGFB3. (sirnalibrary.com)
Chondrosarcoma is the second most common primary sarcoma of bone. (biomedcentral.com)
We developed and characterized three cell lines, derived from conventional grade III chondrosarcoma (L835), and dedifferentiated chondrosarcoma (L2975 and L3252) of bone. (biomedcentral.com)
Chondrosarcoma is a malignant bone neoplasm characterized by the deposition of a hyaline cartilaginous extracellular matrix. (biomedcentral.com)
L835 is derived from a grade III conventional chondrosarcoma, while L2975 and L3252 originate from dedifferentiated chondrosarcomas of bone. (biomedcentral.com)
Coordinated chondrocyte proliferation and differentiation are required for normal endochondral bone growth. (biomedcentral.com)

Gene expression profiles have indicated that during progression chondrosar coma cells shift from a differentiated state in reduced grade tumors to a state additional very similar to early chondrogenic differentiation phases of mesenchymal precursor cells in higher grade tumors. (sirnalibrary.com)
The Sox9 gene is required for the differentiation of mesenchymal precursor cells to chondrocytes and, together with the related L-Sox5 and Sox6 proteins, controls chondrocyte-specific gene expression [ 4 , 5 ]. (biomedcentral.com)

The morphology in the cells and the composition with the matrix in central chondrosarcoma propose par allels in between differentiation phases of tumor cells and of ordinary chondrocytes. (sirnalibrary.com)
In central chondrosarcoma TGFB signaling is active accord ing to detection of nuclear phosphorylated Smad2. (sirnalibrary.com)

Once they embed themselves into the cartilage matrix, they grow the cartilage matrix by growing more cartilage extracellular matrix rather than by dividing further. (wikipedia.org)
KEGG pathways enrichment and PPI network indicate four major pathways, including extracellular matrix -receptor interaction, focal adhesion, phosphatidylinositol 3-kinase (PI3K)-Protein kinase B (Akt), and Ras signaling pathways were involved in the degeneration of cartilage. (nature.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Primary dedifferentiated chondrosarcoma tissues were obtained at the time of surgery and subjected to primary tissue culture. (cancer-genetics.org)
The existence of cancer stem cells (CSCs) within CRC tissues was recently suggested to play importance roles for this process. (bvsalud.org)

The Wnt signaling are evolutionarily conserved and implicated in cell proliferation and sarcomagenesis. (oncotarget.com)
Contrast agent effects on cell viability and proliferation were analyzed using MTT analysis. (biomedcentral.com)
When compared with the control group, both SSEA-1 protein expression and cell proliferation were lowest in the Gd-DPT group ( P = 0.000). (biomedcentral.com)
However, the mere fact that a drug used solely for diagnostic purposes may repress chondrocyte cell proliferation should be carefully considered by clinicians. (biomedcentral.com)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) commercial kit (Vybrant MTT cell Proliferation assay, cat. (biomedcentral.com)
This study aims at investigating the mechanism by which GINS1 affects HCC cell proliferation and stemness. (bvsalud.org)
Our study found the high expression of GINS1 and E2F1 in HCC, and overexpressed GINS1 markedly enhanced the sphere formation and proliferation of HCC cells, while silencing GINS1 led to the opposite results. (bvsalud.org)
The results of the rescue experiment suggested that overexpressed E2F1 could offset the suppressive effect of GINS1 silencing on HCC cell stemness and proliferation. (bvsalud.org)
We demonstrated that the transcription factor E2F1 accelerated cell proliferation and stemness in HCC by activating GINS1 transcription. (bvsalud.org)
Growth and development of endochondral bones is controlled through the highly coordinated proliferation and differentiation of growth plate chondrocytes [ 1 - 3 ]. (biomedcentral.com)

As suggested in the name, mesenchymal progenitors originate from the mesoderm. (wikipedia.org)

Afterward, the proliferative capacity and stemness of HCC tumor cells were determined through colony formation, cell counting kit-8, and sphere formation assays. (bvsalud.org)

An important genetic component of this process is Sox9, a HMG box transcription factor, which marks progenitor cells for chondrogenic differentiation. (wikipedia.org)
Inactivation of the Sox9 gene will result in the loss of all Cartilage, and thus Chondroblast, formation. (wikipedia.org)

Taken together, these outcomes indicate that endogenous TGFB signaling is respon sible for a substantial degree of apoptotic signaling in FET cells as abrogation of TGFB inhibitory signaling within the FET DN cells rendered the cells much more resistant to apoptosis. (sirnalibrary.com)

Rhabdomyosarcoma (RMS) is a malignant tumor of mesenchymal origin and comprises the largest category of soft-tissue sarcomas both in children and adolescents. (biomedcentral.com)

As proven by detection of nuclear Smad proteins, the TGFB and BMP signaling pathways are lively in many cells of the growth plate and they are controlled by tight temporal and regional patterns of expression of your components in the TGFB superfamily and of their receptors. (sirnalibrary.com)
In this study, the effects of short-term exposure of human primary chondrocyte cell cultures to gadolinium and/or iopromide contrast agents were examined by assaying for stage-specific embryonic antigen-1 (SSEA-1) protein expression (a chondrogenic differentiation marker), cell viability, toxicity, and proliferation. (biomedcentral.com)
Cell differentiation status was assessed according to SSEA-1 protein expression. (biomedcentral.com)
The induced CD44 expression in 5-FU treated CRC cells could also be decreased after the expression of ELF3 was inhibited. (bvsalud.org)
Moreover, it could be observed that the expression of ELF3 is significantly higher in CD44+ CRC cells. (bvsalud.org)
Taken together, our results suggested that CD44 expression might be regulated by ELF3 and could be induced after 5-FU treatment of CRC cells. (bvsalud.org)
The protein expression levels of GINS1, E2F1, and cell stemness-related genes (SOX-2, NANOG, OCT4, and CD133) were detected by Western blot. (bvsalud.org)

These cells are extremely important in chondrogenesis due to their role in forming both the chondrocytes and cartilage matrix which will eventually form cartilage. (wikipedia.org)
The correl ation of the differentiation stage of chondrosarcoma cells for the degree of malignancy in the tumors indi cates that signaling pathways that management standard chondrogenesis may perhaps possess a regulatory perform within the progression of those tumors. (sirnalibrary.com)

Patient demographic data, histologic diagnosis, and chondrosarcoma grade were recorded. (rsna.org)

It has been found that expressing this gene will result in the suppression of the differentiation of chondroblasts. (wikipedia.org)
General inactivation or activation of these gene doesn't turn all affected cells into one type or another. (wikipedia.org)
Cucchiarini, M., Madry, H. Biomaterial-guided delivery of gene vectors for targeted articular cartilage repair. (uniklinikum-saarland.de)
In contrast, the Runx2 gene (also known as Cbfa1) is essential for differentiation of osteoblasts, but also promotes hypertrophic chondrocyte differentiation [ 5 , 7 ]. (biomedcentral.com)

Chondrosarcoma represents a heterogeneous group of tumors. (biomedcentral.com)

The purpose of the present study is to assess the possible cytotoxic effects of IPM and Gd-DPT on chondrocytes by blindly comparing the use of these agents in human primer cell cultures at the molecular level in vitro. (biomedcentral.com)
Here we demonstrate that Atf3 mRNA levels increase during mouse chondrocyte differentiation in vitro and in vivo . (biomedcentral.com)

It has been suggested that differentiating embryonic stem cells with these growth factors could prevent stem cells, once injected into potential patients, from forming teratomas, or stem cell caused tumors. (wikipedia.org)

These processes are regulated by a large number of endocrine, paracrine and autocrine hormones and growth factors that, to a large part, act on chondrocyte cell surface receptors. (biomedcentral.com)

In contrast to these effects suggesting that TGFB signaling may very well be involved in chondrosarcoma progression, data demonstrating lively BMP signaling in chondrosarcoma tissue are lacking. (sirnalibrary.com)
These repressors could down-regulate CRE-dependent transcription and thus cause delay of cell cycle progression and/or promote cell cycle exit during terminal differentiation. (biomedcentral.com)

Chondroblasts, or perichondrial cells, is the name given to mesenchymal progenitor cells in situ which, from endochondral ossification, will form chondrocytes in the growing cartilage matrix. (wikipedia.org)
Chondroblasts are called chondrocytes when they embed themselves in the cartilage matrix, consisting of proteoglycan and collagen fibers, until they lie in the matrix lacunae. (wikipedia.org)
Collagen Type II fibers are responsible for giving the future cartilage matrix its tensile strength. (wikipedia.org)
Proteoglycans resist the compression generally put upon cartilage and generate the swelling pressure responsible for stress shielding the matrix from compression loading. (wikipedia.org)

To date, three Wnt signaling pathways have been characterized including the canonical Wnt/β-catenin signaling, the noncanonical planar cell polarity pathway (Wnt/PCP) and the Wnt/Ca 2+ pathway [ 7 , 8 ]. (oncotarget.com)

In the canonical Wnt/β-catenin signaling, a Wnt ligand binds to a cell surface receptor complex consisting of the members of Frizzled family and the transmembrane protein LRP 5 and 6 to prevent phosphorylation and degradation of β-catenin by the GSK3β/APC/Axin destruction complex. (oncotarget.com)

These cells, when forming from the mesoderm, specifically form from embryonic stem cells via induction through BMP4 and fibroblast growth factor FGF2 while the fetus is inside the womb. (wikipedia.org)

The knee cartilage samples were collected from gender- and age-matched KBD (n = 9) and OA (n = 9) patients. (nature.com)

If the Wnt/ β-Catenin pathway is upregulated, then endochondral ossification is encouraged which promotes ossification of the formed cartilage. (wikipedia.org)

This is a thin layer of connective tissue which protects cartilage and is where chondroblasts help to expand cartilage size whenever prompted to by hormones such as GH, TH, and glycosaminoglycans. (wikipedia.org)
Extraskeletal myxoid chondrosarcoma (EMC), a soft-tissue sarcoma with unique clinicopathologic features and characteristic chromosomal translocations, is extremely rare in the pediatric population. (cancer-genetics.org)
Tumor-tissue derived from three resected specimens derived from one conventional and two dedifferentiated chondrosarcomas were used for culture. (biomedcentral.com)

This study supports application of a standardized measuring technique for improved distinction of benign osteochondromas from chondrosarcomas, with a cartilage cap thickness of 2 cm or greater as the determinant of chondrosarcoma. (rsna.org)

High-grade conventional chondrosarcoma and dedifferentiated chondrosarcoma have a poor outcome. (biomedcentral.com)

Within finished cartilage, collagen fibers compose 10-20% of the volume, water 65-80%, and the proteoglycan-hyaluronic acid aggregates the remaining portion. (wikipedia.org)
The structure of these fibers, like the majority of collagen fibers, forms a triple helix structure. (wikipedia.org)
After washing, the cells were transferred into small collagen-coated culture flasks and cultured in RPMI1640 supplemented with 20% heat inactivated Fetal Calf Serum (Gibco, Invitrogen Life-Technologies, Scotland, UK), 1% L-glutamax, and 1% penicillin/streptomycin (100U/mL). (biomedcentral.com)

It is important to note that perichondrium, and thus chondroblasts, are not found on the articular cartilage surfaces of joints. (wikipedia.org)

In an immunohisto chemical research, a correlation of TGFB1 and TGFB2 to the grade of chondrosarcoma continues to be described. (sirnalibrary.com)
To validate a technique for reproducible measurement of the osteochondroma cartilage cap with computed tomography (CT) and magnetic resonance (MR) imaging and to reevaluate the correlation of the thickness of the cartilage cap with pathologic findings to improve noninvasive differentiation of benign osteochondromas from secondary chondrosarcomas. (rsna.org)

The development of chemoresistance in colorectal cancer (CRC) cells was usually thought to be inevitable as a result of continuing exposure to chemotherapeutic drugs. (bvsalud.org)

The cells maintained in monolayer cultures exhibited constant growth, spheroid formation capacity, and invasion ability. (cancer-genetics.org)

Further, changes in cell morphology in relation to the control group were evaluated using inverted light microscopy, environmental scanning electron microscopy (ESEM), and 3-tesla magnetic resonance imaging. (biomedcentral.com)

Therefore, ATF3 induction appears to facilitate cell cycle exit and terminal differentiation of chondrocytes. (biomedcentral.com)

Due to the proliferative nature of Chondroblasts, cells compose a larger portion of the composition than what is normally found within completed cartilage. (wikipedia.org)

This is a feature shared by, in particular, grade I chondrosarcomas. (biomedcentral.com)

Based on the stable culturing properties of these cell lines and their genotypic profile resembling the original tumors, these cell lines should provide useful functional models to further characterize chondrosarcoma and to evaluate new treatment strategies. (biomedcentral.com)
It is because of these features that there is an urgent need for model systems in pre-clinical research aimed at evaluating new targeted treatment strategies for chondrosarcoma [ 4 ]. (biomedcentral.com)
RNA-seq analysis revealed that ELF3, one of the members of ETS (E26 transformation-specific) transcription activator family, was increased along with CD44 after 5-FU treatment of CRC cells. (bvsalud.org)

citation needed] It is important to note here that these genes are not the only factors which determine whether chondroblasts will form. (wikipedia.org)
We had shown earlier that transcription of the cell cycle genes cyclin D1 and cyclin A in chondrocytes is dependent on CREs. (biomedcentral.com)

Results from dual-luciferase reporter assay revealed that the transcription of CD44 could be activated by ELF3 in CRC cells. (bvsalud.org)

GO analysis shows that the differential abundant proteins are associated with cell junction and signal transducer activity from extracellular to intracellular. (nature.com)

To confirm that PI3KAKT signaling was linked to cell survival in FET DN cells we treated cells with LY294002, a potent inhibitor of PI3K. (sirnalibrary.com)

The outcomes showed that phosphorylation selleck chemicals of AKT was decreased in FET cells relative to FET DN cells below each GFDS strain and ordinary development situations. (sirnalibrary.com)

Cell cultures with no drug exposure were used as the control group. (biomedcentral.com)

among which embryonal and alveolar RMSs are the most common ones under the age of 20, while pleomorphic and spindle cell variants of the tumor may also occur in adults, with a peak at the 4th-5th and 6th -7th decades of lifetime, respectively. (biomedcentral.com)

Cells were injected into nude mice to establish their tumorigenic potential. (biomedcentral.com)
We show that the three cell lines have distinct migrative properties, L2975 had the highest migration rate and showed tumorigenic potential in mice. (biomedcentral.com)

however, over the last 10 years many of the key transcriptional regulators of chondrocyte differentiation have been identified. (biomedcentral.com)

Anatomy57

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