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  • resistance
  • Zeocin and other related chemicals in the bleomycin family of compounds are primarily used in molecular biology as an antibiotic, especially for the selection of eukaryotic cell lines when used in conjunction with vectors containing a selectable marker for zeocin resistance. (wikipedia.org)
  • Multiple independent complementation groups of Chinese hamster ovary (CHO) cells have been isolated, and this gene restored UV resistance to cells of complementation group 4. (wikipedia.org)
  • protein
  • Since serum is a major cost factor and an obstacle to protein purification, our goal was to develop a large-scale TGE process for Chinese hamster ovary (CHO) cells in the absence of serum. (epfl.ch)
  • Ding K, Han L, Zong H, Chen J, Zhang B, Zhu J (2017) Production process reproducibility and product quality consistency of transient gene expression in HEK293 cells with anti-PD1 antibody as the model protein. (springer.com)
  • Bves is a highly conserved, transmembrane protein that is involved in cell adhesion, cell motility, and most recently has been shown to play a role in vesicular transport. (wikipedia.org)
  • Bves interacts with GEFT, a protein that modulates Rho GTPases, Rac1 and Cdc42, which are important for cell motility through modulation of the actin cytoskeleton. (wikipedia.org)
  • Signal peptides function to prompt a cell to translocate the protein, usually to the cellular membrane. (wikipedia.org)
  • In cholesterol-depleted cells, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi apparatus. (wikipedia.org)
  • GTPase
  • Taken together, these data demonstrate that Bves modulates Rho GTPase signaling cascades through interaction with GEFT to affect cell movement and morphology. (wikipedia.org)
  • optimal
  • To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. (thermofisher.com)
  • genes
  • Studies have demonstrated that silencing certain target genes involved in certain CHO cell apoptotic and metabolic pathways resulted in 40 to 60% improved cell viability as compared with untreated cells. (thefreedictionary.com)
  • Baser B, Spehr J, Bussow K, van den Heuvel J (2016) A method for specifically targeting two independent genomic integration sites for co-expression of genes in CHO cells. (springer.com)
  • construct
  • In this study, we investigated three sites with potential high transcriptional activities: C12orf35, HPRT, and GRIK1, to determine the possible transcriptional hot spots in CHO cells, and further construct a reliable site-specific integration strategy to develop recombinant cell lines efficiently. (springer.com)
  • receptor
  • Activation of the kisspeptin receptor is linked to the phospholipase C and inositol trisphosphate second messenger cascades inside the cell. (wikipedia.org)
  • In addition to explaining the underlying pathology of this disease, their work uncovered a fundamental aspect of cell biology - Receptor-mediated endocytosis. (wikipedia.org)
  • human
  • Moving to the University of Texas Health Science Center in Dallas, now the UT Southwestern Medical Center, Brown and colleague Joseph L. Goldstein researched cholesterol metabolism and discovered that human cells have low-density lipoprotein (LDL) receptors that extract cholesterol from the bloodstream. (wikipedia.org)
  • processes
  • Disruption of Bves results in decreased cell speed and increased cell roundness, which are cell processes modulated by the Rho GTPases, Rac1 and Cdc42. (wikipedia.org)
  • medium
  • Freezing medium: Cell medium (F-12 or DMEM/F-12) without selection agents or antibiotics plus 20% FBS and 10% DMSO (dimetylsulfoxide, sterile). (openwetware.org)
  • However the problem I am having, is that many cells just don't attach to the flask and remain floating, even after changing medium every 2 days so I don't think it has to do with medium. (protocol-online.org)
  • Cell lines from different sources, and different clones of the same cell line, may have highly specific nutritional requirements and may therefore prefer one medium over another. (thermofisher.com)
  • Regulation
  • Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. (wikipedia.org)
  • Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism. (wikipedia.org)
  • Phenotype
  • Upon Bves disruption, cell rounding is increased, a phenotype indicative of decreased adhesion and disruption of integrin function. (wikipedia.org)
  • productivity
  • Site-specific integration of transgenes into CHO hot spots is an ideal strategy to overcome these challenges since it can generate isogenic cell lines with consistent productivity and stability. (springer.com)
  • In comparison with a random integration control, all the targeted integration cell lines showed higher productivity, among which C12orf35 locus was the most advantageous in both productivity and cell line stability. (springer.com)
  • fibronectin
  • Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. (wikipedia.org)
  • migration
  • VAMP3 is important for recycling of integrins during cell migration and is also necessary for exocytosis of transferrin. (wikipedia.org)
  • Thus, Bves is important for VAMP3-mediated vesicular transport underlying cell migration and transferrin recycling. (wikipedia.org)
  • process
  • We offer an extensive breadth of process development capabilities from cell line and strain development, using our proprietary pAVEway[TM] and CHO cell line systems, to process development, analytical development, clinical and commercial manufacturing. (thefreedictionary.com)
  • Deep sequencing demonstrated that there was low level of off-target mutations caused by CRISPR/Cas9, but none of them contributed to the development process of transgene cell lines. (springer.com)
  • selection
  • However, by conventional random integration strategy, development of a high-expressing and stable recombinant CHO cell line has always been a difficult task due to the heterogenic insertion and its caused requirement of multiple rounds of selection. (springer.com)
  • Stable cell lines were generated successfully after a single round of selection. (springer.com)
  • target
  • Targets for radiation-induced cell death: target replication during the cell cycle evaluated in cell. (biomedsearch.com)
  • Our results demonstrated the feasibility of C12orf35 as the target site for exogenous gene integration, and strongly suggested that C12orf35 targeted integration mediated by CRISPR/Cas9 is a reliable strategy for the rapid development of recombinant CHO cell lines. (springer.com)
  • cellular
  • The results show that only the hydroxylated derivatives (NMHE and 9-hydroxyellipticinium), which covalently bind to nucleic acids, increase the cellular sensitivity to ionizing radiations, whereas the non-hydroxylated derivative, 2N-methyl-ellipticinium, has no influence on cell radiosensitivity. (biomedsearch.com)
  • cholesterol
  • Since 1993, Drs. Brown, Goldstein, and their colleagues have described the unexpectedly complex machinery by which cells maintain the necessary levels of fats and cholesterol in the face of varying environmental circumstances. (wikipedia.org)