Loading...
  • oxidative
  • The phenotypical and physiological implications revealed from our experiments highlight the important roles of CGLD1 in maintaining structure and function of PSII as well as in protection of Chlamydomonas under photo-oxidative stress conditions. (frontiersin.org)
  • construct
  • Localization was investigated using two different constructs, a histidine-tagged version of the Chlamydomonas rppH and a histidine-tagged 5'rppH-GFP construct. (uio.no)
  • biosynthesis
  • For decades, C. reinhardtii has fueled research on photosynthetic gene function ( 3 ), and is an indispensable reference for studying the carbon concentrating mechanism ( 4 , 5 ), ciliary function and composition ( 6 ⇓ - 8 ), lipid metabolism and prospects of biofuel production ( 9 ⇓ - 11 ), carotenoid biosynthesis ( 12 ) and nutrient starvation responses ( 13 ⇓ - 15 ). (pnas.org)
  • mRNA
  • In addition, Chlamydomonas chloroplasts contain a number of mRNA specific translation factors that are not found in bacteria. (springer.com)
  • Our working hypothesis is that the siRNA signal transmitted to Chlamydomonas has the same effect on the mRNA as the physical siRNA. (scirp.org)
  • Here we present the optimization of the siRNA signal transduction to Chlamydomonas while we measure the growth rate and then fine tune the conditions measuring the amount of mRNA produced under the influence of the signal specific for the mRNA. (scirp.org)
  • plasmid
  • Despite its auspicious features, nuclear gene targeting in C. reinhardtii through homologous recombination (HR)-mediated plasmid integration occurs at prohibitively low levels ( 18 , 19 , 23 ⇓ ⇓ ⇓ ⇓ - 28 ). (pnas.org)
  • recombination
  • We demonstrate an approach to bypass inefficient gene targeting via homologous recombination and achieve homology-directed DNA replacement in C. reinhardtii . (pnas.org)
  • growth
  • Bonente G, Pippa S, Castellano S, Bassi R, Ballottari M (2012) Acclimation of Chlamydomonas reinhardtii to different growth irradiances. (springer.com)
  • 2009). With lower levels of MAA7, Chlamydomonas reinhardtii can grow in the presence of the prodrug 5-Fluoroindole (5-FI), because less 5-Fluoroin-dole can be converted to the toxic 5-Fluoro-L-tryptophan (5-FT). We find a 24% ( ± 5%) increase of growth with the signal versus no signal. (scirp.org)
  • efficiency
  • Here we report that single-step codelivery of CRISPR/Cpf1 ribonucleoproteins with single-stranded DNA repair templates results in precise and targeted DNA replacement with as much as ∼10% efficiency in C. reinhardtii . (pnas.org)
  • content
  • Phylogenetic reconstruction of changes in plastid genome content revealed that an accelerated rate of gene loss also characterized the Chlamydomonas/Chlorella lineage, a phenomenon that might be independent of the proliferation of SDRs. (plantcell.org)
  • different
  • Localization was investigated using two different constructs, a histidine-tagged version of the Chlamydomonas rppH and a histidine-tagged 5'rppH-GFP construct. (uio.no)
  • conditions
  • It is discussed how the transition from state 1 to state 2 can protect C. reinhardtii in high-light conditions and how this differs from the situation in plants. (pnas.org)