Although mammals do not produce chitin, they have two functional chitinases, Chitotriosidase (CHIT1) and acidic mammalian chitinase (AMCase), as well as chitinase-like proteins (such as YKL-40) that have high sequence similarity but lack chitinase activity. (wikipedia.org)
However, one example of a Class V chitinase showed two chitin binding domains in tandem, and based on the gene sequence, the cysteine-rich N-terminal seemed to have been lost during evolution, probably due to less selection pressure that caused the catalytic domain to lose its function. (wikipedia.org)
As a result of studies conducted in our research group on chitin synthesis and considering the lack of information on chitinase ants, we propose, in this project, the production of different chimeric proteins (containing chitinase catalytic domain and modules for binding in chitin) and the study of its catalytic function against chitin and analysis of the products through HPLC and mass spectrometry. (fapesp.br)
Maximum chitinase production was obtained at 37˚C, pH 7.5, 3 M NaCl and 1% colloidal chitin. (org.rs)
The improvement of transformants was investigated by quantification of mRNA using real-time quantitative polymerase chain reaction (RT-PCR) and measurement of chitinase activity in the medium containing colloidal chitin as the carbon source. (ijbiotech.com)
Proteins1
The genes and proteins were predicted and annotated based on the genome sequence. (springeropen.com)
Single chitinase3
This is the release of four single chitinase deletion mutants in Salmonella enterica serovar Typhimurium LT2. (researchgate.net)
Exons are represented by had been when compared with identify the exon-intron distribution of every single chitinase-like genes. (gsk-3inhibitor.com)
Overexpression of single chitinase can improve the mycoparasitic potential of the biocontrol agent ( 13-15 ). (ijbiotech.com)
Mammalian1
The aim was to performin silico upstream promoter identification and analysis to determine types of transcription factors and their binding domains crucial in transcribing the human CHI3L1 gene along with multiple sequence alignments and detailed phylogenetic analysis of eight mammalian species of the CHI3L1 gene. (academicspub.com)
1.141
Chitinases are also present in plants - for example barley seed chitinase: PDB: 1CNS, EC 3.2.1.14. (wikipedia.org)
Similarity3
BLAST result showed that the cloned fragment has 93% similarity to chitinase of S. plymuthica strain 3Re4-18 (CP012097) and others. (scholarsresearchlibrary.com)
Furthermore, domain analysis showed that the putative chitinase has only one domain, GH18_chitinase, as catalytic domain resembles high similarity to the ChiA domains. (scholarsresearchlibrary.com)
By sequence similarity analysis and functional assays from the species Streptomyces lividans TK23, S. coelicolor A3(2) and S. glaucescens GLA.O, first hints for a widespread lack of MalP and presence of AmlE in the class Actinobacteria were given. (frontiersin.org)
Transcriptome3
The availability of next-generation sequencing technology has circumvented this barrier for non-model organisms by enabling transcriptome assembly from high-throughput RNA sequencing (RNA-seq) data [ 9 , 10 ]. (biomedcentral.com)
Thus the first Illumina-based de novo transcriptome sequencing and analysis of the chicken parasite Plasmodium gallinaceum was performed to assess the evolution of essential Plasmodium genes. (biomedcentral.com)
Orthologues identified by transcriptome sequencing were characterized using phylogenetic, ab initio protein modelling and comparative and population-based methods. (biomedcentral.com)
Enzymes8
Chitinases were also classified based on the amino acid sequences, as that would be more helpful in understanding the evolutionary relationships of these enzymes to each other. (wikipedia.org)
CLUSTALX software was utilized to conduct the alignment of amino acid sequences ofof the catalytic domains CLUSTALX software was utilized to conduct the alignment of amino acid sequences the catalytic domains in GH18 family members enzymes. (gsk-3inhibitor.com)
The production of different enzymes by S. marcescens as virulence factors has also been reported, including chitinase, lipase, chloroperoxidase and an extracellular protein, HasA. (microbiologyresearch.org)
Chitinase is one of the most important hydrolytic enzymes with a variety of biotechnological applications. (org.rs)
The bacteria exhibit potent collagen-digesting properties, and genome sequencing has identified novel metalloprotease, collagenase and chitinase enzymes representing potential virulence factors. (biomedcentral.com)
Foliar squirt of ANE elicited activity of defense-related enzymes, including peroxidase (PO), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and chitinase in carrot [12] and cucumber [13]. (biotech2012.org)
Cell wall degrading enzymes like chitinase from T. harzianum are important means for fungal pathogen inhibition. (ijbiotech.com)
In the present study, the genetic variability of E. dispar strains based on the SREDP gene locus was analyzed using RFLP-nested PCR and sequencing of the obtained genes. (scialert.net)
ALDH1 Formulation Insects 2021, 12,9 ofInsects 2021, 12, x FOR PEER REVIEW9 ofAll of the 14 chitinase-like genes in B. tabaci were matched to a particular scaffold sequence in B. tabaci genome and also the exon-intron distribution were shown (Figure 4). (gsk-3inhibitor.com)
Exon-intron distributions of 14 B. tabaci chitinase-like genes. (gsk-3inhibitor.com)
Genomic sequences and putative cDNA sequences quences were in comparison with ascertain the exon-intron distribution of every chitinase-like genes. (gsk-3inhibitor.com)
Developmental Expression Patterns of Chitinase-Like Genes in B. tabaci 3.3. (gsk-3inhibitor.com)
10,200 protein-encoding genes were predicted based on the genome sequence. (springeropen.com)
Nucleotide3
The nucleotide sequences of Serine-Rich Entamoeba histolytica Protein (SREHP) gene have already exhibited stable and significant polymorphism in the gene studies. (scialert.net)
The first nucleotide sequences have been shown the stable and significant polymorphism in the Strain Specific Gene (SSG) and the Serine-Rich E. histolytica Protein (SREHP) gene. (scialert.net)
The analysis of the nucleotide sequences showed that the 77 accessions of A. thaliana were partitioned into two major differentiated haplotype classes (MDHCs). (biomedcentral.com)
Putative3
In order to detail investigated chitinase from S. plymuthica, a putative chitinase II fragment has been successfully isolated from S. plymuthica strain UBCR_12. (scholarsresearchlibrary.com)
And as the gene sequences of the chitinases were known, they were further classified into six classes based on their sequences. (wikipedia.org)
Characteristics that determined the classes of chitinases were the N-terminal sequence, localization of the enzyme, isoelectric pH, signal peptide, and inducers. (wikipedia.org)
Class II chitinases did not have the cysteine-rich N-terminal but had a similar sequence to Class I chitinases. (wikipedia.org)
Class III chitinases did not have similar sequences to chitinases in Class I or Class II. (wikipedia.org)
Among chitinases, chitinase 42 plays a major role in the inhibition of pathogen growth ( 7 ). (ijbiotech.com)
Homology1
however it only has 25% sequence homology to IL-4 and is capable of IL-4 independent signaling. (biofargo.com)
The production of chitinase in strain BS5 accompanied growth, started at the logarithmic phase and increased to its maximum level at the beginning of the stationary phase. (org.rs)
Based on 16S rRNA gene sequencing, strain RMB7 was identified as Bacillus specie. (biomedcentral.com)
Novo1
For de novo sequencing of the Agrobacterium sp. (uni-bielefeld.de)
Airway1
Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation. (biofargo.com)
Phylogenetic1
In this study, chloroplast DNA sequence variability was used to infer phylogenetic relationships among Arabidopsis thaliana accessions from around the world, with the emphasis on samples from China. (biomedcentral.com)
CHI3L12
Because studies from our laboratory have demonstrated that chitinase 3-like-1 (CHI3L1) stimulates ACE2 and Spike (S) priming proteases that mediate SC2 infection, studies were undertaken to determine if interventions that target CHI3L1 are effective inhibitors of SC2 viral variant infection. (elifesciences.org)
YKL-40, encoded by Chitinase-3-like protein 1 (CHI3L1) gene, is predominantly an inflammation-based secretory glycoprotein in type-2-diabetes. (academicspub.com)
Genome sequence1
The complete genome sequence of Agrobacterium sp. (uni-bielefeld.de)
Isolation1
Isolation was performed by PCR-based cloning strategy by applying a pair of primer, specifically designed to amplify chitinase II fragment. (scholarsresearchlibrary.com)
Analysis4
Based on its 3D-modeling structure analysis we assumed that the cloned chitinase fragment belongs to the subfamily of Chi indicating the similar function to SpChiD. (scholarsresearchlibrary.com)
Combining deep sequencing and bioinformatics analysis, the percent of the common sequences in total sRNAs was 92.79%, but the number of unique sRNAs in the flying queens was more than that in non-flying queens. (chinaagrisci.com)
Analysis of sequences deposited in databases has already been started and two pairs of oligonucleotides have been acquired. (fapesp.br)
Typing methods used to study the epidemiology of S. marcescens include biotyping, bacteriocin typing, phage typing, plasmid analysis, polymerase chain reaction amplification of enterobacterial repetitive intergenic consensus sequences (ERIC-PCR) and ribotyping. (microbiologyresearch.org)
Patterns2
From those isolates, six distinct DNA patterns were observed after PCR-RFLP of the nested PCR, whereas sequencing showed 8 different patterns among the isolates. (scialert.net)
Ghosh reported 5 different SREDP sequencing patterns from 5 isolates and showed high genetic variation among the E. dispar in compare with E. histolytica (Ghosh et al . (scialert.net)
Genus1
This study revealed the strong potential of the genus Natrinema to produce chitinase at high salt concentrations without Mg 2+ requirement. (org.rs)
Polymorphism1
The polymorphism of the Serine-Rich Entamoeba dispar Protein (SREDP) gene among 8 isolates obtained from Iranian cyst carriers were analyzed by a nested PCR-RFLP followed by sequencing of the PCR products. (scialert.net)
Species2
Generating a reference sequence for P. tecunumanii and characterizing pathogenesis related gene families in these two pine species is an important step towards unravelling the pine- F. circinatum interaction. (biomedcentral.com)
Predicted protein sequences encoded by the assembled transcriptomes were clustered with reference proteomes from 92 other species to identify pathogenesis related gene families in P. patula, P. tecunumanii and other pine species. (biomedcentral.com)
Amplification1
Besides, observing chitinase function in culture media, through an additional molecular test the presence of the chitinase gene in chitinase-producing strains was also confirmed by PCR amplification. (org.rs)
Activity2
The overexpressing transformants of Chit42 displayed higher levels of chitinase activity to inhibit S. sclerotiorum growth compared with the wild type. (ijbiotech.com)
The results indicated that the value of the chitinase activity (126.42 + 0.07 U/mL) of Chit42-9 increased 64.17 fold. (ijbiotech.com)
Data2
In order to clarify the biological and pharmacological mechanisms of O. sinensis , we carried out the genome sequencing of H. sinensis, anamorph of O. sinensis , for the first time, described the annotation and gene expression, and analyzed the complete sequencing data (Li et al. (springeropen.com)
In particular, our data shed light on the sequence of events regarding the initiation and progression of neuropathological processes relevant in AD. (unige.ch)
High1
The sRNAs expression differences between the two groups were analyzed by using a high-throughput sequencing method. (chinaagrisci.com)
Genetic1
In particular, the availability of a sequenced genome, protocols for genome transformation and editing, the presence of a canopy, and a proplastid-to-chloroplast developmental gradient across the leaf blade, together with the unique large collections of natural genetic diversity and mutant populations, make barley a perfect model for chloroplast research. (mdpi.com)
Plant1
Apply treatment of okra plant life with chitosan elevated the full total phenolic content material and elevated polyphenol oxidase, peroxidase, chitinase, and -1,3-glucanase [26]. (biotech2012.org)
Disease1
Neurofilament light (NfL), chitinase-3-like protein 1 (YKL-40), and neurogranin (Ng) are biomarkers for Alzheimer's disease (AD) to monitor axonal damage, astroglial activation, and synaptic degeneration, respectively. (unige.ch)
Class1
They find clone 10, a Class I chitinase, in the seed aleurone during development. (wikipedia.org)
Regions3
Regions underlined would be the four conserved motifs represented by the sequences KxxxxxGGW, FDGxDLDWEYP, MxYDxxG and GxxxWxxDxDD. (gsk-3inhibitor.com)
Regions underlined are the 4 conserved motifs represented by BtCht9 both had only 1 exon and no introns were present, although BtCht10 had the most the sequences KxxxxxGGW, FDGxDLDWEYP, MxYDxxG and GxxxWxxDxDD. (gsk-3inhibitor.com)
In an investigation on the maternal origins of A. suecica , 12 cpDNA regions were sequenced for 25 A. thaliana accessions, which were mainly collected from Scandinavia [ 25 ]. (biomedcentral.com)