Chiral phases analytical phase separation. Medical search. Frequent questions

The vernacular for a chiral stationary phase chromatography column remains a "Pirkle column," and colleagues and alumni fondly remember the "Pirkle-ator" solvent stills in RAL, and how he was always available for late-night mentorship with his big mug of tea in hand. (illinois.edu)
Wang Z, Wang S, Zhu F, Chen Z, Yu L, Zeng S (2012) Determination of enantiomeric impurity in besifloxacin hydrochloride by chiral high-performance liquid chromatography with precolumn derivatization. (springer.com)
Supercritical fluid chromatography (SFC) has become popular in the field of enantioselective separations. (mipdatabase.com)
5. Aboul-Enein HY, Ali I. Polysaccharide-based chiral stationary phases, Chapter 2 in chiral separation by liquid chromatography and related technologies. (jddtonline.info)
Lead Surprisingly, chiral chromatography could not be performed in 1980 because there was no single chiral stationary phase on the market. (chiralpedia.com)
But as the 1980s came to a close, chiral chromatography began to get more and more attention, thanks in large part to the efforts of Okamoto's institute in Japan, Pirkle and Armstrong's teams in the US, Schurig and König in Germany, Lindner in Austria, and Francotte in Switzerland. (chiralpedia.com)
Lead A wide range of chiral compounds have been examined as chiral selectors with regard to their ability to separate enantiomers using chromatography as part of the development of CSPs. (chiralpedia.com)
Separation of isomeric metabolites of carbamazepine by liquid chromatography and high resolution accurate mass. (ilmexhibitions.com)
Chiral Stationary-Phase Optimized Selectivity Liquid Chromatography: a Novel Approach for the Separation of Mixtures of Enantiomers. (ilmexhibitions.com)
Liquid chromatography (LC) uses mobile phases that typically contain organic solvents. (edu.au)
The modes selected were reversed phase LC versus capillary zone electrophoresis and electrokinetic chromatography, chiral LC versus chiral electrokinetic chromatography, and size exclusion chromatography versus capillary gel electrophoresis. (edu.au)
Several approaches are considered for 'greening' analytical chromatography, where small solvent savings multiplied across the hundreds or thousands of users found in a typical pharma company can add up to significant waste reduction. (ddw-online.com)
In this article we focus on challenges and opportunities for greening chromatography, adsorption and other separation technologies currently used in the pharmaceutical industry. (ddw-online.com)
The technique of chromatography was invented by botanist Mikael Tswett in 1903, and originally used for the separation of plant pigments (8,9). (ddw-online.com)
Chromatography relies on the selective partition of different molecules between a stationary phase and a mobile phase, as depicted in Figure 1. (ddw-online.com)
Liquid chromatography, most commonly used for purification in the pharmaceutical industry (10), typically employs modified silica or polymer particles as a solid phase, with water, organic solvents, or a mixture of the two as a mobile phase. (ddw-online.com)
Although originally invented by Tswett as a purification technique, chromatography has evolved into one of the most widely used analytical technologies in the pharmaceutical industry. (ddw-online.com)
Analytical chromatography is typically performed using a stationary phase column of 4.6mm diameter and 25cm length at a mobile phase flow rate of about 1mL/min. (ddw-online.com)
In contrast, preparative chromatography is carried out using much larger columns, sometimes in excess of 1 metre diameter, with mobile phase flow rates of many litres per minute. (ddw-online.com)
However, given the sheer number of analytical chromatography instruments in use throughout the pharma industry, the greening of analytical chromatography remains an important focus as well. (ddw-online.com)
High performance liquid chromatography (HPLC) is one of the most commonly used analytical techniques in pharma today10. (ddw-online.com)
Lux Cellulose-3 and Cellulose-4, unique and complementary chiral stationary phases, are now available in a 10 µm particle size for preparative and batch chromatography applications. (labwrench.com)
Phenomenex also offers several free tools for chiral chromatography. (labwrench.com)
Different methods are available for the separation of small molecules including High Performance liquid chromatography (HPLC) , liquid chromatography (LC) , gas chromatography (GC) , thin-layer chromatography (TLC) and capillary electrophoresis (CE). (sigmaaldrich.com)
Getting the best possible results in the high-performance liquid chromatography (HPLC), UHPLC or LC-MS analysis of small molecules depends on the selection of the best suitable stationary phase and mobile phase conditions. (sigmaaldrich.com)
For separation of polar compounds hydrophilic interaction chromatography (HILIC) and normal-phase chromatography are suitable as well, with HILIC being the preferred method. (sigmaaldrich.com)
For the separation of ionic compounds, ion-exchange separation modes and for inorganic anions or cations, ion chromatography can also be used. (sigmaaldrich.com)
Drug substances and counterions are determined by HPLC (often on reversed-phase columns) and by ion chromatography (IC), respectively. (chromatographyonline.com)
For this September issue of The Application Notebook should be reaching our audience of analytical chemists, lab managers, and other industry professionals just as the busiest time of the year ramps up in the world of chromatography. (chromatographyonline.com)
High-Performance Liquid Chromatography (HPLC) is a sophisticated analytical technique widely used in scientific research and industries to separate, identify, and quantify components within a mixture. (acebioscience.com)
High-Performance Liquid Chromatography (HPLC), a cornerstone of modern analytical chemistry, operates on fundamental principles that drive its unparalleled separation capabilities. (acebioscience.com)
Overall, the principles of High-Performance Liquid Chromatography (HPLC) underscore its significance in modern research and analytical applications, ensuring its continued prominence as a powerful technique in the scientific realm. (acebioscience.com)
The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples. (lu.se)
UHPSFC , ultra- pacted by other parameters, such as the addition of the mod- high performance supercritical fluid chromatography ifier and the change of the mobile phase density [20]. (lu.se)
1979. Separation of methyl parathion and fenitrothion metabolites by liquid chromatography. (cdc.gov)

They are suitable for separations of a wide range of chiral compounds, cis-trans isomers, and geometric isomers. (ymcamerica.com)
Regis Reflect™ chiral columns are rugged polysaccharide phases suitable for a wide range of chiral compounds. (cubecounty.com)
Reflect™ coated amylose chiral columns are high performance polysaccharide chiral phases suitable for a wide range of chiral compounds in normal phase HPLC and SFC modes. (cubecounty.com)

Synopsis The development of chromatographic methods for the separation of chiral drugs presents a significant challenge, necessitating a comprehensive understanding of the compounds' chemical properties and the factors influencing their separation. (chiralpedia.com)
In this review, we compared the analytical performance of different modes of LC and CE for the analysis of the same compounds. (edu.au)
This is particularly important for the production of (new) analytical-grade standards, used as reference materials, and for the execution of clinical trials to test the biological activity of the single compounds that could potentially be used as new drugs or active pharmaceutical ingredients (APIs). (chromatographyonline.com)
They encompass a large family of chiral and achiral secondary plant metabolites, directly produced by the plant in an acidic form, with more than 150 compounds discovered so far. (chromatographyonline.com)
This can be explained by the difficulty involved in isolating sufficient purified quantities of these compounds because of their relatively small concentrations in cannabis plants and extracts-hence the need for accurate and high-throughput purification methods able to isolate sufficient quantities of pure cannabinoids for the production of standards for use in medicinal, analytical, and regulatory applications (8,13). (chromatographyonline.com)
The successful resolution of chiral compounds requires more than one stationary phase and the more phases you have to choose from, the better your chances of resolving enantiomers," explains Marc Jacob, Ph.D., product manager for Phenomenex. (labwrench.com)
This group has a greater than 90 percent success rate in finding a chiral column that will separate the toughest chiral compounds. (labwrench.com)
SMT WCX Bulk Packings are silica-based Weak Cation eXchange packing materials developed for separation of cationic compounds. (thomassci.com)
It is well suited for the separation of neutral, acidic and weak basic compounds.This sorbent is packed into a Hibar® 125-4 HPLC column. (thomassci.com)
Other aspects such as speed, sample matrix, and number of compounds define the best suitable base material for the stationary phase. (sigmaaldrich.com)
Supel™ Carbon LC U/HPLC columns packed with porous graphitic carbon (PGC) particles, used for the HPLC, UHPLC, UPLC, and LC-MS separation of polar or charged compounds at high temperatures (up to 250 ˚), pressures up to 700 bar, and the mobile phase pH range of 1-14. (sigmaaldrich.com)
By choosing the appropriate stationary phase and mobile phase composition, analysts can fine-tune the separation, enabling the detection and quantification of even trace amounts of target compounds. (acebioscience.com)

His research was in the areas of organic chemistry and analytical chemistry, particularly in molecular recognition and enantiomer separation using chiral stationary phases (CSPs). (illinois.edu)
Bioanalytical chemistry and resolution of chiral drugs owe Bill a great debt. (illinois.edu)
The last part of the paper gives an overview of applications of enantioselective SFC in analytical chemistry, in both analytical and preparative scales. (mipdatabase.com)
Is Science Lead for Analytical Chemistry within the Process and Analytical Chemistry area at Merck Research Laboratories in Rahway, New Jersey. (theanalyticalscientist.com)
Is a Professor Emeritus at The Institute of Analytical Chemistry, University of Vienna, Austria. (theanalyticalscientist.com)
CDs are employed in various fields of analytical chemistry, due to their tendency to form reversible inclusion complexes and recognize chiral analytes. (chiralpedia.com)
Admission to the Master of Science programme in chemical engineering at Karlstad University, and at least 90 ECTS credits programme courses completed or attended with at least 75 ECTS cr completed, including analytical chemistry 7.5 ECTS cr, or equivalent. (kau.se)
Analytical Chemistry , 89 (3), 1945-1954. (univie.ac.at)
Separation technologies in the pharmaceutical industry offer an attractive target for green chemistry improvements (1-3). (ddw-online.com)
It is these waste mobile phase solvents that offer a significant target for green chemistry and engineering improvements. (ddw-online.com)
Despite the relatively small amount of solvent used for a typical analytical HPLC instrument (~1mL/min) the fact that there may be hundreds or even thousands of these instruments operating within a single pharmaceutical company makes the cumulative use of solvent for analytical HPLC a significant green chemistry concern. (ddw-online.com)
Researchers and industry professionals alike rely on HPLC to ensure the safety and efficacy of drugs, study environmental pollutants, and analyze food and beverage components, making it a cornerstone of modern analytical chemistry. (acebioscience.com)

Unique, proprietary, phase coverage provides excellent peak shape and improved resolution versus leading chiral phases. (cubecounty.com)

YMC Chiral ART phases are available in packed columns for analytical scale separations. (ymcamerica.com)
Reflect coated phases are available in a range of particle sizes, allowing analytical scale separations up through large scale preparative separations. (cubecounty.com)

The work was originally inspired by Pirkle's demonstration that enantiomers could be distinguished by NMR spectroscopy in the presence of chiral solvating agents. (illinois.edu)
UHPLC separation of R - and S -enantiomers of Besifloxacin hydrochloride on a new Chiralpak IE-3 column consisting of an immobilized amylose tris(3,5-dichlorophenylcarbamate) chiral stationary phase on 3 µm silica was studied. (springer.com)
Andrea F, Michaela V, Janka Mydlova JL, Jan K (2006) Interconversion of oxazepam enantiomers during HPLC separation. (springer.com)
Mosiashvili L, Chankvetadze L, Farkas T, Chankvetadze B (2013) On the effect of basic and acidic additives on the separation of the enantiomers of some basic drugs with polysaccharide-based chiral selectors and polar organic mobile phases. (springer.com)
Polysaccharide chiral columns are the most widely used type of chiral stationary phases (CSPs) to separate enantiomers. (cubecounty.com)
Chiral stationary phases (CSPs) The most convenient and most popular analytical methodology to assess chiral purity is the direct separation of enantiomers on so-called chiral stationary phases (CSPs). (chiralpedia.com)
Moreover, despite the large number of discovered cannabinoids, only roughly 20% are available as analytical standards, with some of them available only as racemic or scalemic mixtures, and not as single enantiomers (8). (chromatographyonline.com)
On SUMICHIRAL OA columns direct separation of various enantiomers can be realized effectively. (thomassci.com)

R -Besifloxacin hydrochloride is a novel chiral broad-spectrum fluoroquinolone developed for treatment of bacterial conjunctivitis and used in clinics due to its higher antibacterial activity compared to S -enantiomer. (springer.com)
The proposed reversed-phase method has been sophisticated and authenticated in accordance with ICH criteria and is capable of quantifying irinotecan enantiomer in irinotecan hydrochloride trihydrate API at trace level concentration. (jddtonline.info)
The relevant oligomers for liquid chromatographic enantiomer separation are composed of 6(α-CD), 7(β-CD), and 8(γ-CD) monosaccharide units, in that order. (chiralpedia.com)
SUMICHIRAL OA columns are high-performance chiral columns for enantiomer separation by HPLC. (thomassci.com)

Chiral separation and determination of enantiomeric purity of the pharmaceutical formulation of cefadroxil using coated and immobilized amylose-derived and cellulose-derived chiral stationary phases. (jddtonline.info)
In 1984, his group discovered excellent chiral recognition abilities with cellulose triphenylcarbamate and, in 1986, its 3,5-dimethyl derivative, which has been commercialized as Chiralcel OD. (theanalyticalscientist.com)
These two new phases join the existing purification media options of Cellulose-1 and Cellulose-2, providing a dependable, high-resolution screening set with a wide range of selectivity. (labwrench.com)
The four 10 µm Lux Cellulose chiral phases benefit from Phenomenex's unique manufacturing process and silica coating technology. (labwrench.com)
Lux Cellulose-1 uses cellulose tris (3, 5-dimethylphenylcarbamate) as the chiral selector. (labwrench.com)
Lux Cellulose-2 provides a second chiral selector, cellulose tris (3-chloro-4-methylphenylcarbamate) providing a unique chlorinated stationary phase with chiral recognition abilities that complement the Lux family of columns. (labwrench.com)
Lux Cellulose-3 utilizes Cellulose tris (4-methylbenzoate) as the chiral selector, a variant cellulose ester derivative that offers unique chiral recognition abilities to provide enantioseparation when other phases fail. (labwrench.com)
Lux Cellulose-4 incorporates Cellulose tris (4-chloro-3-methylphenylcarbamate) as the chiral selector - a chlorinated cellulose phenylcarbamate derivative that offers unique chiral recognition abilities. (labwrench.com)

They are known to bind /interact stereoselectively with small molecules reversibly, making them extremely versatile CSPs for chiral separation of drug molecules. (chiralpedia.com)
Donor-acceptor-type CSPs capitalize on synthetic or semi-synthetic chiral low-molecular-weight chiral selectors capable of recognizing chiral analytes by complementary assembly of nonionic attractive interactions. (chiralpedia.com)

YMC Chiral ART Polysaccharide Phases exhibit high stereoselectivity. (ymcamerica.com)
Polysaccharide phases deliver familiar chiral separation behaviorAll available Chiral ART Polysaccharide Phases - immobilized types - are available in 3μm in addition to 5, 10, and 20μm particle sizes. (ymcamerica.com)
Reflect polysaccharide immobilized chiral columns are made using a unique production process of immobilizing the chiral selector on high purity silica gel. (cubecounty.com)
Reflect chiral phases have been developed to match or exceed the performance of legacy polysaccharide chiral columns. (cubecounty.com)
The broad versatility observed with the Whelk-O 1 column, compares favorably with polysaccharide-derived chiral stationary phases and in many cases offers alternate selectivity. (cubecounty.com)
In addition, because of its covalent nature, this chiral phase is compatible with all commonly used mobile phases, including aqueous systems-a distinct advantage over coated polysaccharide chiral stationary phases. (cubecounty.com)
Enantioseparation of ß-carboline derivatives on polysaccharide- and strong cation exchanger-based chiral stationary phases. (univie.ac.at)

Enantiomeric separation of docetaxel starting material by chiral LC using amylose-based stationary phase. (jddtonline.info)
7. Aboul-Enein HY, Ali I. Studies on the effect of alcohols on the chiral discrimination mechanisms of amylose stationary phase on the enantioseparation of nebivolol by HPLC. (jddtonline.info)
9. Khan M, Viswanathan B, Rao DS, Reddy R. Chiral separation of frovatriptan isomers by HPLC using amylose based chiral stationary phase. (jddtonline.info)

The separation was accomplished under normal-phase conditions using a mobile phase composed of dichloromethane:isopropyl alcohol:trifluoroacetic acid:diethylamine (90:10:0.3:0.05 v/v/v/v) under isocratic mode of elution at 15 °C. The interactions between the analytes, additives, and the stationary phase were discussed and the most probable separation mechanism was proposed. (springer.com)

Pirkle's alcohol, an anthracene derivative of trifluoroethanol, is perhaps one of the most famous chiral shift reagent in nuclear magnetic resonance spectroscopy, and can be used to determine the absolute configuration and the enantiomeric purity of chiral molecules. (illinois.edu)

The latter two, OD and AD, are still some of the most popular chiral stationary phases used in HPLC and SFC. (theanalyticalscientist.com)

Bill's final papers on chiral stationary phases came out not too long ago, in 2011 and 2016. (illinois.edu)

And though our four gurus don't attempt to predict the potability of milk from a mirror world, they keenly address the broader significance of chirality, the major milestones in chiral analysis, and where the field is heading. (theanalyticalscientist.com)

In 1982, PTrMA-coated silica gel was commercialized as the first synthetic polymer-based chiral column. (theanalyticalscientist.com)
LiChrosorb® RP-8 is a reliable irregular shaped silica gel carriers with reversed phase properties. (thomassci.com)

Immobilizing the selector improves the stability of the chiral phase and broadens the range of mobile phase options. (cubecounty.com)

From low-molecular-weight chemicals to polymers with both synthetic and biological origins, the chiral molecules investigated as potential CSs span almost the full spectrum of chemical and structural diversity. (chiralpedia.com)
The HPLC analysis of small molecules most often is performed in reversed-phase separation mode. (sigmaaldrich.com)

Despite the success of linear swimmer models in capturing the essential physics of MIPS, they fail in describing the commonly observed chiral swimmers. (aip.org)
Next part deals with chiral separation systems available in SFC, namely with the commonly used chiral stationary phases. (mipdatabase.com)

Various particle sizes and column dimensions offer outstanding cost effectiveness from analytical separation to preparative separation. (ymcamerica.com)

Diol-120, 200, and 300 are suitable for separation or molecular weight determination of proteins with molecular weights of 10,000 to 1,000,000. (thomassci.com)
Generated PEGylated proteins were separated from unmodified proteins using different reversed phase medias (JupiterÂ® C4 and JupiterÂ® C18). (chromatographyonline.com)
In these studies it was found that the Jupiter C18 media provided the best separation of PEGylated proteins from their unmodified counterparts. (chromatographyonline.com)
Such results further clarify good method starting points for developing analytical and preparative separations of PEGylated proteins. (chromatographyonline.com)

Beitrag in 41st International Symposium on High Performance Liquid Phase Separations and Related Techniques - HPLC 2014, New Orleans, USA / Vereinigte Staaten. (univie.ac.at)

There is increasing demand from both pharmaceutical companies and regulatory bodies for increasingly sensitive analytical techniques that can provide the highest possible assurance that a product is of acceptable quality and will provide the promised efficacy safely. (pittcon.org)
It is important for pharmaceutical companies to have the necessary analytical methodologies in place early in the development process so potential impurities are identified early on and are not a surprise at the time approval is sought. (pittcon.org)
Opportunities for reducing solvent consumption and waste generation in separation sciences used to support industrial pharmaceutical discovery and development are presented and discussed in this article. (ddw-online.com)
The separation and quantitation of drug substances and counterions are two important determinations in the pharmaceutical industry (1). (chromatographyonline.com)

Journal of Separation Science , 36 (17), 2826-2837. (univie.ac.at)

The selection and effects of mobile phase solvents, additives, organic modifiers, and column temperature on retention, separation, and resolution were investigated and optimized. (springer.com)
Immobilized Phase - exhibits similar separation behavior, while broadening separation flexibility by enabling the use of a wider range of solvents. (ymcamerica.com)

Properties and interaction possibilities of the chiral separation systems are described. (mipdatabase.com)
This powerful method relies on the interaction between the sample's components and the stationary phase inside a column, as the mobile phase (liquid solvent) passes through it. (acebioscience.com)
At its core, HPLC involves the interaction of sample components with a stationary phase and mobile phase within a specialized column. (acebioscience.com)

One of the most intriguing phenomena in active matter has been the gas-liquid-like motility-induced phase separation (MIPS) observed in repulsive active particles. (aip.org)
For example, while the gas-liquid-like phase separation in equilibrium requires cohesive interactions, 1 active colloids can undergo motility-induced phase separation (MIPS) resulting from the combination of self-propulsion and steric repulsion. (aip.org)
A reversed-phase ion pair liquid chromatographic method (RP-LC) for the determination of dehydroascorbic acid (DHA) and ascorbic acid (AA) and also acetaminophen, which is combined in pharmaceuticals, is proposed and validated. (shengsci.com)
Liquid phase separations are major players in chemical analysis. (edu.au)
The stationary phase, typically composed of silica or polymer materials, serves as a solid matrix, while the mobile phase acts as a liquid solvent. (acebioscience.com)

Rapid separation of the analyte from sample matrix was achieved in less than 60s using a Cromolith RP-18e monolithic column using UV detection at 220 nm. (shengsci.com)
High efficiency separations at any pH The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. (thomassci.com)
The HPLC column is packed with either fully porous silica particles, superficially porous silica particles, polymeric particles or consists of a monolithic silica rod as the stationary phase. (sigmaaldrich.com)
These probes ensure that the columns have been properly deactivated, contain the correct amount of stationary phase, and have the same relative retention as the last column purchased. (chromatographyonline.com)
When a sample is introduced into the mobile phase and pushed through the column by a precise pump, its constituents interact differently with the stationary phase based on their chemical properties, such as polarity and hydrophobicity. (acebioscience.com)

This task is further complicated by the presence of process-related impurities that can disrupt the separation process and compromise the quality of the results. (chiralpedia.com)

Individually, these chiral swimmers exhibit intriguing phenomena such as gravitaxis and 21 abnormal transportation. (aip.org)

Among many other awards, Pirkle was the recipient of the American Chemical Society's Award in Separations Science and Technology in 2004. (illinois.edu)
Pirkle phases contain π-electron donor or acceptor rings substituted with H-bonding moieties. (chiralpedia.com)

Consequently, analytical techniques are needed to characterize the product rapidly with a high level of accuracy, sensitivity and resolution. (pittcon.org)
High resolution greatly improves preparative loading, leading to greater productivity and higher purity separations. (cubecounty.com)
The one-handed helical polymer (PTrMA) exhibited unexpected high chiral recognition ability. (theanalyticalscientist.com)
Based on Core Enhanced Technology, Accucore columns provide fast, high-resolution separations - without the elevated backpressures required by sub-2µm particles. (thomassci.com)
ODS-A is a high coverage, fully endcapped C18 phase which exhibits exceptional batch-to-batch reproducibility. (thomassci.com)
Modern HPLC pumps offer high precision and pressure capabilities, allowing scientists to optimize separations for various applications. (acebioscience.com)

On the Phenomenex website, users are able to use the online chiral application search to quickly and easily search over 2,000 chiral applications by drawing the compound structure or by searching the application name. (labwrench.com)

Consequently, the lack of a universally accepted standardized analytical method for the chemo- and enantio-selective analysis of phytocannabinoids and the limited number of cannabinoid reference standard materials make it difficult to obtain a comprehensive characterization of the sample in terms of purity, identification, and concentration of the single species. (chromatographyonline.com)
Phenomenex also offers free chiral screening services from PhenoLogix, a full-service analytical support laboratory that specializes in chiral method development and small-scale purifications. (labwrench.com)

For HPLC the typical particle size of the stationary phase ranges from 3 µm to 5 µm, for UHPLC smaller particles sizes, typically 2 µm or below, are used. (sigmaaldrich.com)

HPLC enables the separation and quantification of complex mixtures by employing a combination of stationary and mobile phases. (acebioscience.com)
One of the major advantages is its exceptional selectivity, which allows for the separation of complex mixtures with unparalleled precision. (acebioscience.com)

Anatomy6

Organisms3

Diseases1

Chemicals and Drugs129

Analytical, Diagnostic and Therapeutic Techniques and Equipment60

Psychiatry and Psychology2

Phenomena and Processes48

Disciplines and Occupations5

Technology, Industry, Agriculture16

Information Science3

Health Care5

Chromatography35

Wide range of chiral compounds3

Compounds12

Chemistry12

Versus leadin1

Scale separations2

Enantiomers8

Enantiomer4

Cellulose8

CSPs2

Polysaccharide7

Amylose3

Analytes1

Enantiomeric purity1

HPLC and SFC1

20161

Chirality1

Silica gel2

Selector1

Molecules2

Commonly2

Particle sizes1

Proteins4

International Symposium1

Pharmaceutical4

Journal of Separation Science1

Solvents2

Interaction3

Liquid5

Column5

Impurities1

Exhibit1

Pirkle2

High6

Applications1

Method2

Typically1

Complex Mixtures2