Chinese Hamster Ovary (CHO) cell lines are preferred host cells for therapeutic protein production. (nih.gov)
Pretreatment of all three CHO cells with dopamine resulted in desensitization of the adenylyl cyclase response, although the maximum desensitization was attenuated by 20 and 40% in the 10260 and 10248 cell lines, respectively. (aspetjournals.org)
Our results suggest that PKA significantly contributes to the desensitization and down-regulation of D 1 receptors in CHO cells and that type II PKA may be the more relevant isoform with respect to regulating D 1 receptor function. (aspetjournals.org)
This thesis was undertaken to better understand the processes of death in CHO cells and to find ways to extend the viability of CHO cultures. (mit.edu)
A majority of CHO cells in culture were found to die by apoptosis, a genetically controlled form of cellular suicide. (mit.edu)
Protein synthesis inhibition in CHO cells led to rapid death, indicating that CHO cells were pre-disposed to death and that survival proteins needed to be continuously synthesized to protect cells from death. (mit.edu)
Caspases, a class of proteins found to be universally important in inducing apoptosis, were found to be activated in apoptotic CHO cells. (mit.edu)
Using these results, a pathway for apoptosis in CHO cells, focusing on the caspases and bcl-2, was suggested. (mit.edu)
Concentrations of insulin, a growth and survival factor used in CHO culture, were observed to drop rapidly in fed-batch cultures of CHO cells. (mit.edu)
1995 K2 CSR) that measures the potential in vitro genotoxicity of LAS Na to cause mutations in mammalian cells shows that LAS Na was not mutagenic to Chinese Hamster Ovary (CHO) cells both in the presence and absence of S9. (europa.eu)
In a cytogenicity study in mammalian cells, conducted according to OECD Test Guideline 473 and in compliance with GLP, the analogous substance dichloro(3-chloropropyl)methylsilane (CAS 7787-93-1) was negative in Chinese hamster fibroblast cells (Hüls AG, 1997, Reliability Score 1). (europa.eu)
Mutagenicity in mammalian cells: negative in CHO K-1 cells (OECD TG 476) (Hüls AG, 1998b). (europa.eu)
Multiple transporters are involved in this uptake as Chinese hamster ovary or HEK293 cells expressing either OATP1B1 ( K m = 1.4-3.5 μM), OATP2B1 ( K m = 0.7-0.8 μM), or OATP1B3 showed significant fluvastatin uptake relative to control cells. (aspetjournals.org)
Recombinant Human Transferrin produced in CHO cells is a glycosylated, polypeptide chain containing having a molecular mass of 76 kDa. (prospecbio.com)
Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
This application note describes the cultivation of Chinese hamster ovary (CHO) suspension cells in the Finesse SmartGlass vessel bioreactor with a maximum working volume of 2.0 L. Using chemically defined minimal media, cell densities of up to 7.44 106 cells/mL were achieved. (bioprocessintl.com)
Similar augmentation of cAMP accumulation after pertussis toxin treatment was observed in Chinese hamster ovary (CHO) cells transfected with, and stably expressing, the CB1 receptor. (jneurosci.org)
Stimulation of cAMP accumulation was not observed after pertussis toxin treatment of CHO cells expressing the human CB2 receptor, suggesting that this novel signaling pathway is unique to the cannabinoid CB1 receptor. (jneurosci.org)
These include bacteria, yeast, and mammalian cells such as CHO. (2bscientific.com)
Using the fluorogenic substrate succinyl-Ala-Ala-Ala-4-methylcoumaryl-7-amide (Suc-Ala-Ala-Ala-MCA), containing the sequence similar to the cleavage sites for proTGF-alpha processing, we identified a putative candidate proTGF-alpha-converting enzyme in the membrane fractions of Chinese hamster ovary (CHO) cells. (nih.gov)
The enzyme was partially purified from membranes of phorbol ester-treated CHO cells. (nih.gov)
Inhibitor profile of the enzyme, including inhibition by serine protease inhibitors and no inhibition by elastatinal, coincides with that previously reported for proTGF-alpha cleavage activity on CHO cells. (nih.gov)
In the cell-free reconstitution system, the membrane-bound Suc-Ala-Ala-Ala-MCA-hydrolyzing enzyme from CHO cells was also activated by phorbol ester in the presence of the soluble fraction, ATP, and vanadate. (nih.gov)
Protein therapeutics are often produced in living host cells such as Chinese Hamster Ovary (CHO) cells. (epivax.com)
CHOPPI was developed in a collaboration between researchers at Dartmouth College, University of Rhode Island and EpiVax Inc. CHOPPI allows users to search through a collection of proteins and genetic information on CHO cells and quickly access data on how immunogenic and "human-like" a protein is in comparison to others. (epivax.com)
[ 8 ] While reports suggested either (1) lack of lithium effects on cAMP synthesis or (2) lithium-induced inhibition of cAMP synthesis, later work in a strain of rat thyroid follicular cells (FRTL-5) and a cell line of Chinese hamster ovaryfibroblasts stably transfected with the human thyrotropin receptor (CHO-TSHR) showed significant potentiation by lithium of the cAMP response to exogenous thyrotropin. (medscape.com)
Recent studies revealed that nano-sized TiO2 can cause inflammatory response in airways of rats and mice, fibrosis or lung tumors in rats and DNA damage in Chinese hamster ovary (CHO) cells, Syrian hamster embryo fibroblasts and human lymphoblastoid cells. (cdc.gov)
Enzyme replacement therapy (ERT) with glucocerebrosidase purified from human placenta was FDA approved in 1991, followed by approval in 1994 of a recombinant form of the enzyme produced in cultured Chinese hamster ovary (CHO) cells, marketed as imiglucerase (Cerezyme). (medscape.com)
Mammalian cell3
One of the main problems in mammalian cell culture systems, including Chinese Hamster Ovary (CHO) cultures, is the inability to maintain viability of these cultures over extended periods of time. (mit.edu)
Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
Commonly used cell lines include Chinese hamster ovary (CHO) mammalian cell expression systems, such as CHOK1. (iptonline.com)
Stably2
To investigate the role of the cAMP-dependent protein kinase (PKA) in the desensitization and down-regulation of the D 1 dopamine receptor, we stably expressed the rat cDNA for this receptor in mutant Chinese hamster ovary (CHO) cell lines deficient in PKA activity. (aspetjournals.org)
WB: PD-L1 stably expressed CHO whole cell lysate. (abcam.com)
Recombinant2
In contrast to recent data with chimeric, recombinant SARS-CoV-2 strains expressing the spike proteins of BA.1 and BA.2 on an ancestral WK-521 backbone 4 , we observed similar infectivity and pathogenicity in mice and hamsters for BA.2 and BA.1, and less pathogenicity compared with early SARS-CoV-2 strains. (nature.com)
The CHO cell line secretes recombinant factor IX into a defined cell culture medium that does not contain hormones, and the recombinant factor IX is purified by a chromatography purification process that does not require a monoclonal antibody step. (globalrph.com)
Plasmid1
The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
Human1
Here we evaluated the replicative ability and pathogenicity of authentic infectious BA.2 isolates in immunocompetent and human ACE2-expressing mice and hamsters. (nature.com)
Genome1
De Groot's company, EpiVax, developed the concept and contributed EpiMatrix analysis of the CHO genome to the collaboration. (epivax.com)
Proteins1
Despite ongoing production process improvements, CHO host cell proteins (CHOPs) can sometimes contaminate the final product. (epivax.com)
Productivity2
Current work is focused on measuring the UPR, product titer, and product quality in a CHO cell line engineered for high productivity of IgG. (aiche.org)
Based on the above results, possible ways to further reduce death and improve productivity in CHO cultures are also suggested. (mit.edu)
Glucose1
In contrast, expression of bcl- 2, a well-characterized anti-apoptotic gene, was able to significantly increase the life of CHO batch cultures in response to both glucose limitation and growth factor withdrawal. (mit.edu)
Expression1
Bcl-2 expression was shown to improve both final product titers and integrated viable cell densities in CHO fed-batch cultures. (mit.edu)
Model2
Discriminating Significant From Insignificant Model Parameters: The Case of a Dynamic CHO Cell Model. (tufts.edu)
We applied this methodology to a dynamic model of Chinese hamster ovary (CHO) cell metabolism (Nolan, 2011). (tufts.edu)
Activity1
The 10248 mutant CHO line lacks type II PKA activity and expresses a defective type I PKA. (aspetjournals.org)
Line2
These pathways were de novo synthesized in 3 kilobase chunks, assembled in yeasto and genomically integrated into a Chinese hamster ovary (CHO) cell line. (elifesciences.org)
Before that happened, our partner CSL had completed multiple large-scale cGMP runs and millions of potential doses, which-for a mammalian CHO cell line-is difficult to do. (genengnews.com)
Glycosylation1
Consequences of the loss of functional beta 4GalT-1, beta 4GalT-6, or both in CHO glycosylation mutants. (nih.gov)
Methods1
It addresses the specific time bottlenecks associated with developing high producing CHO cell lines and reviews various methods that are employed to alleviate these bottlenecks. (columbia.edu)
Cell lines1
Most Chinese hamster ovary (CHO) cell lines will grow in both perfusion and fed-batch modes. (biopharminternational.com)
Exposure1
Results of recent studies in animals indicate an increased incidence of laryngeal cancer in hamsters and nasal cancer in rats following exposure to acetaldehyde. (cdc.gov)
Genetic1
The genetic sequence of the Chinese hamster ovary (CHO) cell line first was published just over a decade ago (1, 2). (bioprocessintl.com)
Process1
This process was used to successfully isolate a CHO cell clone with the capability of amplifying a transgene targeted to a specific location in the genome and thus produce higher quantities of protein in a shorter time period. (columbia.edu)
Studies1
Long-term inhalation studies of acetaldehyde produced laryngeal cancers in hamsters and nasal cancers in rats. (cdc.gov)
Increase1
Potential future experiments are described which may isolate such sequences and ultimately increase the number of transgenes integrated into the CHO cell genome. (columbia.edu)
Research1
His research focuses on integrating multiomic datasets to understand Chinese hamster ovary (CHO) cell behavior for applications in cell line engineering and optimal. (terrapinn.com)
Technology1
The second project also utilizes SSR technology to integrate many copies of a transgene into many recombination sites in the CHO genome. (columbia.edu)