• The ECFP and EYFP-expressing transgenic ES cells and mice that we have generated provide sources of cells and tissues for combinatorial, double-tagged recombination experiments, chimeras or transplantations. (biomedcentral.com)
  • We employ a variety of experimental systems for our studies ranging from molecular biology to transgenic and knockout mice. (stanford.edu)
  • Methods: To address this question, transgenic Non-Obese Diabetic (NOD) mice which express insulin in the liver were made using the Phosphoenolpyruvate Carboxykinase (PEPCK) promoter to drive the mouse insulin I gene (Ins). (deepdyve.com)
  • The transgenic PEPCK-Ins NOD mice became diabetic at 20-25 weeks of age, with blood glucose levels of 24.1 ± 1.7 mmol/l. (deepdyve.com)
  • Haematoxylin and eosin staining of liver sections from these transgenic NOD PEPCK-Ins mice revealed the absence of an infiltrate of immune cells, a feature that characterised the pancreatic islets of these mice. (deepdyve.com)
  • person to synthesise, store and secrete insulin in the same manner as a pancreatic β cell is a potential therapy of type The liver of the transgenic PEPCK-Ins NOD mice were 1 diabetes. (deepdyve.com)
  • To survey the ease with which motor neuron disease (MND) can be transmitted, we injected spinal cord homogenates prepared from paralyzed mice expressing mutant superoxide dismutase 1 (SOD1-G93A and G37R) into the spinal cords of genetically vulnerable SOD1 transgenic mice. (docksci.com)
  • Homogenates from paralyzed mice expressing the G37R variant were among those that transmitted poorly regardless of the strain of recipient transgenic animal injected, a finding suggestive of strain-like properties that manifest as differing abilities to transmit MND. (docksci.com)
  • These mice are generated by transplanting human hepatocytes into albumin enhancer-driven urokinase-type plasminogen activator transgenic/severe combined immunodeficiency (uPA/SCID) mice, leading to replacement of the host hepatocytes. (wur.nl)
  • In contrast, pathways connected to DNA synthesis were downregulated by fenofibrate in chimeric mice with hepatocyte humanized livers yet upregulated by fenofibrate in normal mouse livers. (wur.nl)
  • To this end we used a HILPDA tissue-specific knockout mice model in macrophages and hepatocytes generated by using LysM-Cre and Alb-Cre transgenic mice, respectively. (wur.nl)
  • Anti-Siglec-8 antibody administration in vivo to humanized and transgenic mice selectively expressing Siglec-8 on mouse eosinophils and mast cells confirmed the in vitro findings, and identified additional anti-inflammatory effects. (mdpi.com)
  • This transposon was utilized by Kraig Labs and the University of Notre Dame to create the world's first transgenic silkworm producing recombinant spider silk. (wikipedia.org)
  • Alongside, we observed a fair correlation between recombinant promoter-driven GUS accumulation with the corresponding uidA-mRNA level in transgenic tobacco. (uky.edu)
  • Histochemical (X-gluc) staining of whole transgenic seedlings and fluorescence images of ImaGene Green™ treated floral parts expressing the GUS under the control of recombinant promoters also support above findings. (uky.edu)
  • Taken altogether, we propose that SA/ABA inducible chimeric/recombinant promoters could be used for strong expression of gene(s) of interest in crop plants. (uky.edu)
  • The antitumor efficacy was studied in mouse mammary carcinoma models (Her2/neu transgenic and 4T1-luc mammary cancers) treated with systemic recombinant protein with/without the depletion of myeloid-derived suppressor cells or intra-tumoral gene electrotransfer (GET). (frontiersin.org)
  • To overcome this problem, SRDX-WRKY75 chimeric repressor transgenic lines were generated. (helsinki.fi)
  • Typical plant transformation protocols to produce transgenic, genetically modified (GM) varieties rely on transgenes, chemical selection, and tissue culture. (frontiersin.org)
  • Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)
  • In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria has gained popularity in mouse transgenic and gene targeting regimes [ 1 ]. (biomedcentral.com)
  • Concerns have been raised over the spread of transgenic DNA by horizontal gene transfer. (i-sis.org.uk)
  • Heterologous expression of the mutated melon ethylene receptor gene Cm-ERS1/70A produces stable sterility in transgenic lettuce ( Lactuca sativa ). (ne.jp)
  • the invention also provides non-human animals and cells comprising a transgene encoding an APP comprising the Swedish mutation and further comprising functionally disrupted endogenous APP gene loci, transgenes and targeting constructs used to produce such transgenic cells and animals, transgenes encoding human Swedish mutation APP polypeptide sequences, and methods for using the transgenic animals in pharmaceutical screening and as commercial research animals for modeling neurodegenerative diseases (e.g. (justia.com)
  • After double digestion with gene nucleotide sequence and orientation. (thetechnoant.info)
  • Various hybrid or combination promoters have been constructed from the CaMV 35S promoter which led to improved expression of transgenes: double 35S promoters (10), a hybrid containing the core 19S promoter from CaMV and the 35S upstream enhancers (11), and combination of CaMV 35S with mannopine synthase elements (12), or with Adh1 - and ocs- promoter elements for expression in monocotyledons (13). (i-sis.org.uk)
  • This paper examines the safety implication of recent revelations on the recombination hotspot of the cauliflower mosaic viral (CaMV) promoter, which is in practically all current transgenic crops released commercially or undergoing field trials. (i-sis.org.uk)
  • Humankind's radically increased ability to deliver precise genotypes in crops thanks to advances in breeding, transformation, transgenics, and editing is helping growers keep pace with increased demand for food and energy. (frontiersin.org)
  • As a precautionary measure, we strongly recommend that all transgenic crops containing CaMV 35S or similar promoters which are recombinogenic should be immediately withdrawn from commercial production or open field trials. (i-sis.org.uk)
  • All products derived from such crops containing transgenic DNA should also be immediately withdrawn from sale and from use for human consumption or animal feed. (i-sis.org.uk)
  • That is all the more urgent as CaMV promoter is in practically all transgenic crops already released commercially or undergoing field trials. (i-sis.org.uk)
  • Thus, this study demonstrates that using proteomic methods on our transgenic model can uncover important in vivo APP-interacting proteins that will provide insights into the biology of APP. (jneurosci.org)
  • Thus, this study demonstrates that our transgenic model can uncover important in vivo APP-interacting proteins that will contribute to our understanding of APP processing in in vivo settings. (jneurosci.org)
  • Our studies demonstrate that DXI improved metabolic alterations observed in transgenic animals fed with HFD in vivo, data in accordance with those obtained at molecular level. (biomedcentral.com)
  • A novel model to study in vivo PPARα activation in human liver is a chimeric mouse carrying human liver cells. (wur.nl)
  • In the present study, we developed a set of three chimeric/hybrid promoters namely FSgt-PFlt, PFlt-UAS-2X and MSgt-PFlt incorporating different important domains of Figwort Mosaic Virus sub-genomic transcript promoter (FSgt, -270 to -60), Mirabilis Mosaic Virus sub-genomic transcript promoter (MSgt, -306 to -125) and Peanut Chlorotic Streak Caulimovirus full-length transcript promoter (PFlt-, -353 to +24 and PFlt-UAS, -353 to -49). (uky.edu)
  • FSgt-PFlt, PFlt-UAS-2X and MSgt-PFlt promoters showed 3.0, 1.3 and 1.0 times stronger activities than the activity of the CaMV35S² (a modified version of the CaMV35S promoter with double enhancer domain) promoter, respectively, in tobacco (Nicotiana tabacum, var. (uky.edu)
  • In this study, by characterization of the dominant maize mutant Barren inflorescence3 ( Bif3 ) we identified a tandem-duplicated copy of ZmWUS1 whose expression is dramatically enhanced by the insertion of a short stretch of chimeric proximal promoter sequence. (nature.com)
  • A study employing NFAT1/NFAT3 chimeric molecules revealed that the region in NFAT3 responsible for NFAT promoter activity inhibition was located within its N-terminal transactivation domain, Ca 2+ -regulatory domain, and DNA-binding domain. (aai.org)
  • We demonstrated that these chimeric/hybrid promoters can drive the expression of reporter genes in different plant species including tobacco, Arabidopsis, petunia, tomato and spinach. (uky.edu)
  • Furthermore, we confirmed that these chimeric promoters are inducible in the presence of 150 μM salicylic acid (SA) and abscisic acid (ABA). (uky.edu)
  • Interestingly, no different phenotypes were found between acd5, cat2, dnd1 and their corresponding wrky75 double mutants in cell death and hydrogen peroxide accumulation detection in Arabidopsis leaves. (helsinki.fi)
  • These chimeric viruses show growth phenotypes similar to nOPV2 and immunogenicity comparable to their parental Sabin strains, but are more attenuated. (cdc.gov)
  • Acute leukemias induced by MLL chimeric oncoproteins are among the subset of cancers distinguished by a paradoxical dependence on GSK-3 kinase activity for sustained proliferation. (stanford.edu)
  • Using this tag, we prepared mild detergent lysates from transgenic mouse brain cortical membrane preparations and isolated a number of previously identified APP-interacting proteins. (jneurosci.org)
  • These are frequently activated by fusion to other transcriptional proteins resulting in chimeric transcription factors. (stanford.edu)
  • To evaluate the function of WRKY75 transcription factor in SA signalling and cell death, three lesion mimic mutants acd5, cat2, dnd1 and their corresponding wrky75 double mutant were used. (helsinki.fi)
  • The invention provides transgenic non-human animals, such as transgenic rodents and transgenic non-human mammalian cells harboring a transgene encoding an APP polypeptide comprising the Swedish mutation. (justia.com)
  • Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species. (patsnap.com)
  • Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. (patsnap.com)
  • Induction of male sterility in transgenic chrysanthemums ( Chrysanthemum morifolium Ramat. (ne.jp)
  • We therefore hypothesized that the selective induction of DNA double-stranded breaks in the X chromosome during male meiosis might be a potential strategy to distort the sex ratio of the progeny towards male mosquitoes, if the normal production of Y-bearing gametes was unaffected ( Fig. 1a ). (nature.com)
  • Transgenic frogs that express the enzyme in the germline can be bred with animals harboring a transposon substrate to generate double transgenic lines where remobilization will occur in the germline in subsequent generations. (biomedcentral.com)
  • The expression of wild-type I-PpoI during spermatogenesis in transgenic mosquitoes causes cleavage of the paternal X chromosome but also results in complete male sterility because the protein's stability and persistence in mature sperm cells leads to subsequent cleavage of the maternal X chromosome in the zygote 9 . (nature.com)
  • That transgenic silkworm is then used as the basis for establishing a genetic line silkworms that also produce spider silk. (wikipedia.org)
  • HLA-B27 transgenic rats develop features of enteropathic arthropathy with arthritis and gut inflammation. (medscape.com)
  • Members of the Pospiviroidae family replicate in the nucleus through double-stranded RNA intermediates, attracting the host RNA silencing machinery. (degruyter.com)
  • Building on these results, the Company analyzed whether a similar additive effect could be achieved when combining varoglutamstat with the Abeta-plaque-specific antibody aducanumab in a double transgenic mouse model overexpressing a variant of the human amyloid precursor protein (APP) and human QPCT (APPSLxhQC). (biospace.com)
  • Furthermore, by generating an amyloid precursor protein/presenilin 1‐green fluorescent protein (GFP) chimeric mouse, we ascertained that the GFP positive microglia identified in the brain were BM‐derived. (nih.gov)
  • Fertilized EpiSC and EpiSC-NT lines largely maintain imprinted gene expression, as did EpiSC-PGA lines that show known paternally expressed genes being silent and known maternally expressed genes consistently showing doubled expression. (biomedcentral.com)
  • Mr Newberry reckons it takes about 18 months to make a transgenic goat that produces a desired therapeutic protein in its milk. (cropchoice.com)
  • As applied in the laboratory, the Cas9 system has two main components, the Cas9 endonuclease and a chimeric single guide RNA (sgRNA) which contains both a short sequence homologous to the gene of interest and sequences necessary for Cas9 interaction 10 . (nature.com)
  • A small 20 base guide RNA (gRNA) can locate Cas9 endonuclease to the targeted site which causes the double stranded break (DSB) in the genomic DNA. (nih.gov)
  • He joined NIAID's Comparative Medicine Branch (CMB) in 2015 and established the Mouse Genetics and Gene Modification (MGGM) section to provide state of the art technologies of mouse genome modification, including CRISPR/cas9 mediated genome editing, generation of transgenic mouse models, gene targeting in ES cells and iPS cells, germplasm cryopreservation and rederivation of mouse lines. (nih.gov)
  • New gene editing tools, such as CRISPR/Cas9 and zinc finger nucleases (ZFNs), are able to produce double-stranded breaks at predefined, highly specific and targeted loci. (ddw-online.com)
  • 2016). For editing the genome, the CRISPR/Cas system makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease that creates the DNA double-strand break (DSB) at the target site, and a donor oligonucleotide or plasmid as the repair material if needed (for knock in models). (addgene.org)
  • Virions are triple-layered and contain a viral genome that consists of 11 segments of double-stranded RNA (dsRNA). (biomedcentral.com)
  • Data from clinical trials for hemophilia B using adeno-associated virus (AAV) vectors have demonstrated decreased transgenic coagulation factor IX (hFIX) expression 6-10 weeks after administration of a high vector dose. (jci.org)
  • Generation of transgenic animal models for human genetic disorders and for mechanisms of gene regulation. (nih.gov)
  • Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans. (alecomm.com)
  • To date, the majority of recombinant glycoproteins in production from mammalian cell lines such as CHO, NS0 and Sp2/0 are monoclonal antibodies, among which are mainly either chimeric, humanized or human Immunoglobulin Gs (IgGs) [6]. (ukdiss.com)
  • 17. Use of the composition of claim 1, or the vector system of claim 2 or any claim dependent thereon, in the production of a non-human transgenic animal or transgenic plant. (patentdocs.org)
  • This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. (alecomm.com)
  • I. a first regulatory element operably linked to a nucleotide sequence encoding a CRISPR-Cas system chimeric RNA (chiRNA) polynucleotide sequence as defined in claim 1, and II. (patentdocs.org)
  • This (transgenic) mutant line expresses PfLSAP2 (PF3D7_0202100) under the control of the Pbuis4 regulatory sequences. (pberghei.eu)
  • The invention provides a "transgenic wheat cell or wheat plant … comprising a chimeric DNA molecule [that] encodes a dsRNA molecule which is capable of inhibiting wheat streak mosaic virus replication," the patent's abstract states. (genomeweb.com)
  • The MGGM Core possesses specialized expertise in state-of-the-art technologies for in vivo gene modification in mouse embryos for the production of transgenic, CRISPR mediated gene knockout (KO), gene knock-in (KI) and conditional gene KO mouse models. (nih.gov)
  • The animals were treated with either varoglutamstat, chimeric aducanumab (chAdu) or a combination of both for 16 weeks. (biospace.com)
  • Moreover, when a drug maker needs to double production, it simply breeds more animals--thereby avoiding spending $300m on a new factory. (cropchoice.com)
  • Each double-PE vector included two expression cassettes for each of the two pegRNAs or sgRNAs. (biomedcentral.com)
  • Efficacy of these vaccines was assessed in mouse malaria challenge models using chimeric P. berghei parasites expressing the relevant Pf antigens. (pberghei.eu)
  • The resulting generation of a double-stranded break markedly increases the rate of HR by orders of magnitude. (ddw-online.com)
  • This transposon was utilized by Kraig Labs and the University of Notre Dame to create the world's first transgenic silkworm producing recombinant spider silk. (wikipedia.org)
  • A firm wishing to produce a therapeutic protein in quantity can contract GTC to create transgenic goats that excrete the desired protein in their milk. (cropchoice.com)
  • According to Thomas Newberry of GTC, creating a herd of transgenic goats costs about $100m--expensive, but still only a third the cost of building a protein production facility. (cropchoice.com)
  • Once the transgenic mammal has matured, however, increasing the volume of protein production is simply a matter of milking. (cropchoice.com)
  • The invention relates to a method for the chemical synthesis of RNA, specifically double-stranded RNAs including siRNAs, according to the patent. (genomeweb.com)
  • Also claimed in the patent is "a process for producing the aforementioned transgenic wheat cell or plant. (genomeweb.com)
  • He was the first to establish transgenic technology at NIH. (nih.gov)
  • Because the rapid process of NHEJ repairs double stranded DNA breaks without using a homologous DNA template, the repair can cause insertion or deletion of base pairs (indels) into the target region. (nature.com)
  • In addition, p19ARF binds to and promotes the degradation of MDM2, the product of the murine double minute 2 gene, and this degradation leads to accumulation of TP53 and to cell cycle arrest. (medscape.com)
  • When applied to the three murine isoforms of Dishevelled, it leads to earlier defects than previously observed in double knock-outs. (biomedcentral.com)