• This gene is located in the cystatin locus and encodes a secreted thiol protease inhibitor found at high levels in saliva, tears and seminal plasma. (wikipedia.org)
  • Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C". FEBS Lett. (wikipedia.org)
  • To analyze the contribution of cysteine proteases to metastasis we have over-expressed in B16 melanoma cells the natural cysteine protease inhibitor, cystatin C. We measured in vitro invasion of cystatin over-expression clones with Boyden chamber type assays. (biomedcentral.com)
  • Cystatin C is a type II cysteine protease inhibitor that is normally secreted from cells [ 10 ]. (biomedcentral.com)
  • Egg white solutions contain cysteine proteinase inhibitor - cystatin, which can be utilized, in the convenient state in liquid form, in some food and pharmaceutical industries. (olsztyn.pl)
  • Cystatin C, a cysteine protease inhibitor, was subject to hydrolysis at two sites when complexed with papain and in the presence of excess papain. (mcmaster.ca)
  • A pH-dependent conformational variability in this region of the inhibitor could explain the differences in the X-ray crystallographic and n.m.r. structures of the homologous chicken cystatin. (mcmaster.ca)
  • Description: Quantitativesandwich ELISA kit for measuring Chicken Interleukin 6, IL-6 in samples from serum, plasma, tissue homogenates. (elisastrip.com)
  • The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. (wikipedia.org)
  • 1989). "Cystatin superfamily. (wikipedia.org)
  • Identification of full-sized forms of salivary (S-type) cystatins (cystatin SN, cystatin SA, cystatin S, and two phosphorylated forms of cystatin S) in human whole saliva and determination of phosphorylation sites of cystatin S". J. Biochem. (wikipedia.org)
  • Cystatin-SA is a protein that in humans is encoded by the CST2 gene. (wikipedia.org)
  • To help establish mechanism of cystatin C action in metastasis we have fused cystatin C to the N-terminus of green fluorescent protein. (biomedcentral.com)
  • Confocal microscopy was used in an attempt to localize cystatin C-GFP protein expression in cells. (biomedcentral.com)
  • This pattern of expression was not anticipated since cystatin C is primarily a secreted protein that should have produced a vesicular localization of fluorescence within the cell. (biomedcentral.com)
  • Figure 2 shows expression of the cystatin C-GFP fusion protein. (biomedcentral.com)
  • Rounding out the remaining protein sources in egg whites, but in minimal amounts, is avidin at 0.05 percent, cystatin at 0.05 percent, ovomacroglobulin at 0.5 percent, ovoflavoprotein at 0.8 percent, ovoglycoprotein at 1 percent and ovoinhibitor. (livestrong.com)
  • Vegetarian participants did have higher levels of triglycerides and a protein called cystatin C, however, which may indicate decreased kidney function. (healthline.com)
  • The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. (wikipedia.org)
  • We used a transcriptomic approach to define the protease and antiprotease genes specifically expressed in the hen liver in relation to vitellogenesis by comparing sexually mature and pre-laying chickens showing different steroid milieu. (biomedcentral.com)
  • Using a 20 K chicken oligoarray, a total of 582 genes were shown to be over-expressed in the liver of sexually mature hens (1.2 to 67 fold-differences). (biomedcentral.com)
  • Showing associations between polymorphisms of c-Ski , c-myc , DGAT1 , AGL , cystatin B and calpastatin genes and selected carcass quality traits of pigs [Urbański et al . (igbzpan.pl)
  • Liquid fractions of egg white were characterised by high inhibitory activity of cystatin. (olsztyn.pl)
  • It results from the conducted examinations concerning chicken breast muscles that lysozyme exhibited inhibitory action towards aerobic and indicator bacteria. (olsztyn.pl)
  • Description: Quantitative sandwich ELISA for measuring Chicken Interleukin 6 (IL-6) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. (elisastrip.com)
  • The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. (wikipedia.org)
  • The importance of the N-terminal region of human cystatin C or chicken cystatin for the kinetics of interactions of the inhibitors with four cysteine proteinases was characterized. (lu.se)
  • The Abs in the kit have high sensitivity and excellent specificity for detection of Cystatin 6 (CST6). (biocheminfo.org)
  • The association rate constants for the binding of recombinant human cystatin C to papain, ficin, actinidin and recombinant rat cathepsin B were 1.1 x 10(7), 7.0 x 10(6), 2.4 x 10(6) and 1.4 x 10(6) M-1.s-1, whereas the corresponding dissociation rate constants were 1.3 x 10(-7), 9.2 x 10(-6), 4.6 x 10(-2) and 3.5 x 10(-4) s-1. (lu.se)
  • The decreased affinity of human cystatin C or chicken cystatin for cysteine proteinases after removal of the N-terminal region is thus due to either a decreased association rate constant or an increased dissociation rate constant, or both, depending on the enzyme. (lu.se)
  • The objective of the study was to investigate the influence of membrane material and membrane cut off on transport and separation properties of ultrafiltration membranes, as well as verification of the possibility of membrane techniques application for the recovery of bioactive components isolated from chicken eggs. (olsztyn.pl)
  • Results show the in vitro invasion of cystatin C over-expressing cells was dramatically inhibited. (biomedcentral.com)
  • Similar results were obtained for intact and N-terminally truncated chicken cystatin. (lu.se)
  • In this study we show over-expression of cystatin C in melanoma cells is associated with reduced metastasis and increased apoptosis in lung tissues. (biomedcentral.com)
  • The expression of cystatin C-GFP fusion was observed in B16F10 melanoma cells following transient transfection of plasmid construct DNA. (biomedcentral.com)
  • A cytoplasmic expression was noted for GFP alone and cystatin C-GFP fusion when transiently expressed in B16 melanoma cells (Figure 1 ). (biomedcentral.com)
  • Microfiltration, using cross flow technique membranes made from polypropylene with 0.2 μm pores, was used as the first step of cystatin isolation from egg white (initial fractionation of egg white). (olsztyn.pl)
  • When membrane cut off point was increased specific activity of cystatin was lowered. (olsztyn.pl)
  • The aim of the study was to assess the effect of lysozyme with varying activity on the microbiological stability and sensory attributes of chicken breast muscles. (olsztyn.pl)
  • Cystatin A incorporates into the cornified cell envelope of stratified squamous epithelial cells and may play a role in bacteriostatic properties of skin. (lookformedical.com)
  • IHC-P analysis of human skin tissue section using GTX04453 Cystatin A antibody [MSVA-461M] HistoMAX. (genetex.com)
  • IHC-P analysis of human rectum mucosa tissue section using GTX04453 Cystatin A antibody [MSVA-461M] HistoMAX. (genetex.com)
  • There are currently no references for Cystatin A antibody [MSVA-461M] HistoMAX (GTX04453) . (genetex.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Cystatin A (CSTA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (resourcerecovery.be)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Cystatin A (CSTA) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (resourcerecovery.be)
  • We have created monomer-stabilized human cystatin C, with an engineered disulfide bond (L47C)-(G69C) between the structural elements that become separated upon domain swapping. (nih.gov)
  • 15. Molecular dynamics simulation to investigate the impact of disulfide bond formation on conformational stability of chicken cystatin I66Q mutant. (nih.gov)
  • The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. (wikipedia.org)
  • The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. (wikipedia.org)
  • Cystatin C Kidney Functional Reserve Predicts CKD Outcomes What could cystatin c kidney functional reserve measurements tell us about patient outcomes in CKD? (medscape.com)
  • This gene is located in the cystatin locus and encodes a secreted thiol protease inhibitor found at high levels in saliva, tears and seminal plasma. (wikipedia.org)
  • Description: Growth Hormone Chicken Recombinant (cGH) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton. (scalegen.com)
  • GH Chicken recombinant is purified by proprietary chromatographic techniques. (scalegen.com)
  • Human cystatin C (HCC) is a family 2 cystatin inhibitor of papain-like (C1) and legumain-related (C13) cysteine proteases. (nih.gov)
  • Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C". FEBS Lett. (wikipedia.org)
  • We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. (bio-techne.com)
  • It illustrates for the first time the fold of monomeric cystatin C and allows verification of earlier predictions based on the domain-swapped forms and on the structure of chicken cystatin. (nih.gov)