Chick embryo cell embryo cells. Medical search. Frequent questions

Our aim is to determine if the same pathway is followed in human cardiomyocytes derived from human inducible pluripotent stem cells. (syr.edu)
To complement the in vivo system of chick embryos, we employ patient omics data, single cell RNA sequencing, human and mouse embryos, human cancer cell cultures, human pluripotent stem cells, chick embryo neural crest-derived in vitro crestosphere cultures, and zebrafish and mouse in vivo models. (lu.se)

Induction of the floor plate during embryogenesis of vertebrate embryos has been studied extensively in chick and zebrafish and occurs as a result of a complex signaling network among tissues, the details of which have yet to be fully refined. (wikipedia.org)
Through chick - quail hybrid experiments as well as genetic interaction experiments in zebrafish, it appears that notochord and floor plate cells originate from a common precursor. (wikipedia.org)
Furthermore, in zebrafish, Nodal signaling is required for differentiation of medial floor plate cells whereas Shh is expendable. (wikipedia.org)
To observe how fever impacts a developing fetus, the researchers studied zebrafish and chicken embryos. (neurosciencenews.com)

In addition to its role as a strong antioxidant, vitamin C regulates the differentiation of several cell lineages. (nih.gov)
Functionally, the structure serves as an organizer to ventralize tissues in the embryo as well as to guide neuronal positioning and differentiation along the dorsoventral axis of the neural tube. (wikipedia.org)
This is supported experimentally in chick, in which floor plate induction, as well as associative ventral nervous tissue differentiation, is mediated by the secreted signaling molecule sonic hedgehog (Shh). (wikipedia.org)
In vitro tissue grafting experiments show that removal of this molecule prevents differentiation of the floor plate, whereas its ectopic expression induces differentiation of floor plate cells. (wikipedia.org)
Later in development during neurulation, extended contact and interaction between notochord and fated floor plate cells causes differentiation, suggesting a cooperative effect between Nodal and Shh signaling. (wikipedia.org)
At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken amniotic epithelial cells / cultured in amniotic epithelial cell conditioned medium not only survived but also showed extensive differentiation. (scielo.org.ar)
Embryo cells modify their cell membrane glycoconjugates during their differentiation. (revmedvet.com)
M�P) Connexin40 (Cx40 or Gja5) (M and N) or EphB4 -lacZ (O and P) in littermate E9.5 Rasip1+/ and Rasip1 / yolk sacs (M and N) or embryos (O and P) showing failed arterial (M and N) and venous (O and P) differentiation in Rasip1 / vessels. (xenbase.org)

Prior to MET initiation, mesenchymal cells exhibit non-polarized distribution of multiple polarity markers, albeit not aPKC. (bvsalud.org)
Rasipl null angioblasts fail to properly localize the polarity determinant Par3 and display defective cell polarity, resulting in mislocalized junctional complexes and loss of adhesion to extracellular matrix ( ECM ). (xenbase.org)

To gain insights into the possible involvement of intracellular vitamin C on early myogenesis, we investigated the regulation of SVCT2 expression in cultures of chick fetal myoblasts. (nih.gov)
The round nucleoli of chick embryo myoblasts, when grown in a culture medium devoid of arginine, unravel in several days into 5-20 µ long, beaded strands termed nucleolar necklaces (NN). (silverchair.com)

In this project the candidate will use chick embryos, patient tissue material, and omics data to investigate how hotspot mutations in susceptibility genes affect healthy embryogenesis, organ formation and initation of the endocrine tumor form paraganglioma. (lu.se)

To complement the in vivo system of chick embryos, we also employ human cell cultures, chick neural crest derived in vitro crestospheres and in vivo mice models. (lu.se)

Cell death in the axial structures, i.e. spinal cord, notochord and surrounding mesenchyme and somites, was analyzed using light and electron microscopy. (karger.com)
Chu-Wang, I.W., R.W. Oppenheim (1978) Cell death of motoneurons in the chick embryo spinal cord. (karger.com)
Previously, we have reported neurotrophism exhibited by human amniotic epithelial cells when transplanted after spinal cord injury in bonnet monkeys. (scielo.org.ar)
2001). In our previous report, we concluded the usefulness of amniotic epithelial cells transplantation in spinal cord injury repair research. (scielo.org.ar)
Recently, our conclusion of usefulness of amniotic epithelial cells transplantation to treat spinal cord injuries has been confirmed in rodent models of spinal cord injury (Zhi-yuan et al. (scielo.org.ar)
Shh is expressed in LMC neurons in developing mouse and chick spinal cord. (elifesciences.org)
B ) ISH analysis of chick Shh combining with IHC of Isl1/FoxP1 and Lhx3 in chick spinal cord. (elifesciences.org)
At HH St.29, Shh is mainly detected in LMCm (Isl1 + /FoxP1 + ) neurons at brachial levels and LMCl (Isl1 - /FoxP1 + ) neurons at lumbar levels but not in motor neurons at thoracic levels of chick spinal cord (dotted outline). (elifesciences.org)
Shh signaling is required for LMC specification in chick spinal cord. (elifesciences.org)
A ) IHC analyses in chick spinal cords electroporated with sh-Shh and sh-vector construct. (elifesciences.org)
C ) Quantification of the relative intensity of Shh ISH signal in motor neurons, % marker + (BrdU, Nkx2.2, Olig2, and cCasp3) cells relative to uninjected side and % motor columns relative to uninjected side of the spinal cord. (elifesciences.org)
C ) Quantification of the number of LMCm (Isl1 + /FoxP1 + ), LMCl (Hb9 + /FoxP1 + or Lhx1 + /FoxP1 + ), MMC (Hb9 + /Lhx3 + ) and HMC (Hb9 + /Isl1 + ) neurons, Olig2 + , Sox2 + , Ki67 + cells and total MNs at cervical level in E12.5 mouse embryonic spinal cord. (elifesciences.org)
Reduced expression of ARHGAP3 does not affect progenitor cell proliferation in Shh -cKO mouse spinal cord. (elifesciences.org)
Two modes of cell migration in the ventral horn of the spinal cord in the chick embryo. (us.es)

Cells Tissues Organs (2001) 169 (4): 347-354. (karger.com)
Where researchers once struggled to connect events at static timepoints, imaging tools now offer the ability to visualize the dynamic form and function of molecules, cells, tissues, and whole embryos throughout the entire developmental process. (cshlpress.com)
Further techniques are organized by the level of visualization they provide, from cells to tissues and organs to whole embryos. (cshlpress.com)
Q�Z0 ) Flk1 -LacZ beta-galactosidase staining of littermate E8.5 (6 somite stage) Rasip1+/ and Rasip1 / embryos and tissues, in whole mount or section. (xenbase.org)

Heme biosynthesis in a chicken hepatoma cell line (LMH): comparison with primary chick embryo liver cells (CELC). (wakehealth.edu)
In this study, antibodies were raised against chicken fat cell membranes. (usda.gov)
These antibodies were used, in tissue culture, to reduce fat cell cluster number, cell size, or cluster size of chicken fat cells. (usda.gov)
Why are chicken embryos typically the go-to for flu vaccine cultivation? (cdc.gov)

The signaling requirements for ventral dermal cell development have not been established in either the chick or the mammalian embryo. (biologists.com)
Mammalian cell lines were subjected to extensive safety testing to establish a cell line that is human pathogens free, while maintaining sufficient vaccine yield. (cdc.gov)

Chick in ovo electroporation is a technique which allows genetic manipulation of the avian embryo. (jove.com)

Figures 323 & 324 from Chapter 13 (Cilia and Flagella) of 'The Cell' by Don W. Fawcett M.D. The outer segments of the rods and cones of the vertebrate retina and many photoreceptors of invertebrates b. (cellimagelibrary.org)

Knock-down of chick Shh did not affect the proliferation (BrdU) or survival (cCasp3) of neural progenitor cells and ventral neural patterning (Olig2 and Nkx2.2). (elifesciences.org)
In addition, we show that, at later stages,Wnt/β-catenin signaling is sufficient for ventral dermal cell specification. (biologists.com)
Consistent with the different origins of dorsal and ventral dermal cells, our results demonstrate both conserved and divergent roles ofβ-catenin/Wnt signaling in dermal development. (biologists.com)

This study investigates MET in vivo in the chick embryo lateral mesoderm , where a multilayered mesenchyme transforms into two parallel epithelial sheets that constitute the coelomic lining of the embryonic body cavity. (bvsalud.org)
2001) had found amniotic epithelial cells conditioned medium showed neurotrophic effect on rat embryonic day 18 (E18) cortical neurons. (scielo.org.ar)
The surgical procedure for delivery of embryonic stem cell-derived endothelial cells to the ischemic hindlimb is demonstrated, with non-invasive tracking by bioluminescence imaging. (jove.com)
E�H) E9.5 littermate mouse embryos showing defects in Rasip1 / embryos (F) and yolk sacs (H). (I�L) Whole-mount Flk1 -lacZ beta-galactosidase staining of Rasip1+/ (I and K) and Rasip1 / (J and L) yolk sacs (I and J) and embryonic heads (K and L), showing narrow Rasip1 / blood vessels that fail to remodel. (xenbase.org)
These cancers originate from cells of the sympathetic nervous system (SNS), in turn derived from the transient embryonic neural crest stem cell population. (lu.se)

The production of several different virus particles in the VV replication cycle represents a coordinated strategy to exploit cell biology to promote virus spread and to aid virus evasion of antibody and complement. (microbiologyresearch.org)

When those neural crest cells were subjected to conditions mimicking a transient fever, the embryos developed craniofacial irregularities and heart defects, including double outlet right ventricle, Tetralogy of Fallot and other outflow obstructions. (neurosciencenews.com)
We focus on the childhood tumor form neuroblastoma, originating from cells of the sympathetic nervous system (SNS) in turn derived from the transient neural crest stem cell population. (lu.se)

Cardiovascular function depends on patent blood vessel formation by endothelial cells (ECs). (xenbase.org)

In order to examine possible differences in mechanisms of action of nifedipine and verapamil, simultaneous recordings of membrane potential and cell motion were made during exposure of cultured chick embryo ventricular cells to these drugs. (biu.ac.il)
Dissociation of progeny vaccinia virus from the cell membrane is regulated by a viral envelope glycoprotein: effect of a point mutation in the lectin homology domain of the A34R gene. (microbiologyresearch.org)
Of 2 glycoproteins on the surface of the RNA viral envelope, one mediates neuraminidase and hemagglutination activity, whereas the other is responsible for fusion to the lipid membrane of the host cell. (medscape.com)

An alternative view proposes that neural tube floor plate cells stem from precursor cells which migrate directly from axial mesoderm. (wikipedia.org)
We analyzed the morphological features of dying cells in the developing axial structures of 5 human embryos between 5 and 8 weeks of postovulatory age. (karger.com)
Later, apoptotic death appeared in all the axial structures, with the exception of the notochord, where some dying cells displayed features of secondary necrosis. (karger.com)
According to our findings, apoptosis seems to be the most frequently observed type of PCD, but it is not the exclusive type of morphological cell death during the development of axial structures in human embryos. (karger.com)

S�V) Transverse sections of E8.5 embryos, through the trunk region, posterior to the heart . (xenbase.org)
1) elucidating the role of neuroblastoma associated protein HIF-2a in neural crest development and its potential role in tumor onset, 2) how trunk neural crest specific genes can function as tumor suppressor genes in neuroblastoma, and 3) develop inducible tumor models using chick embryos. (lu.se)

Placental stem cells. (scielo.org.ar)

Clarke, P.G.H. (1990) Developmental cell death: Morphological diversity and multiple mechanisms. (karger.com)
The technique can be applied to study many different cell types and their roles at various developmental stages. (neurosciencenews.com)

This video demonstrates neural tube electroporation in HH 10 chick embryos. (jove.com)
Each set of chick electroporation experiments in this figure was repeated independently at least three times with 6 to 10 embryos. (elifesciences.org)
Embryos were harvested 4 days post electroporation (dpe). (elifesciences.org)

By virtue of expressions of glial and neural surface markers and capability of neurotransmitter metabolism, amniotic epithelial cells are considered as candidate cell type for transplantation strategies to treat neurological disorders. (scielo.org.ar)
Amniotic epithelial cells were believed to secrete an 'Epidermal Growth Factor (EGF) - like' factor and exact identification was not made. (scielo.org.ar)
It is interesting to note that while factor secreted by amniotic epithelial cells resembles EGF and/or FGF-2 in its biological action, known isoforms of them were not detected. (scielo.org.ar)
Considering the biological closeness between EGF and FGF-2, results indicate the possibility of a novel isoform of these growth factors secreted by amniotic epithelial cells. (scielo.org.ar)
Amniotic epithelial cells were known to express some of neuronal and glial cell markers (Sakuragawa et al. (scielo.org.ar)
Transplantation of amniotic epithelial cells in various regions of central nervous system such as caudate nucleus (Bankiewicz et al. (scielo.org.ar)
Amniotic epithelial cells transplantation in Parkinson model of rats was found to reverse the condition and prevent death in neurons (Kakishita et al. (scielo.org.ar)
We had also outlined various biological and social advantages by which, amniotic epithelial cells transplantation excels its precedent viz. (scielo.org.ar)
Based on the neurotropism exerted by amniotic epithelial cells conditioned medium, a diffusible neurotrophic factor produced by them had been suggested as a possible cause apart from direct cell-to-cell effects (Uchida et al. (scielo.org.ar)

High temperatures caused severe craniofacial defects in chicks, dramatically shortening the upper beak compared to normal controls. (neurosciencenews.com)

Adipocyte precursor cells were obtained for primary culture from 18-day chick embryos by collagenase digestion. (usda.gov)

As axons not destined to cross the midline project through the neural tube they are repelled by the ligand slit which is expressed in the cells of the floor plate. (wikipedia.org)

Clarke, P.G.H. (1984) Identical populations of phagocytes and dying neurons revealed by intravascularly injected horseradish peroxidase, and by endogenous glutaraldehyde-resistant acid phosphatase in the brains of chick embryos. (karger.com)
B ) Knock-down of chick Shh reduced the number of LMCl (Hb9 + /FoxP1 + ) neurons but had no effect on other motor columns such as LMCm, MMC, and HMC and consequently reduced the number of total MNs compared to the uninjected control side. (elifesciences.org)

Chick embryos are easily accessible, and their nervous system development is similar to that of human embryos at comparable stages. (lu.se)

Fetal damage occurs through destruction of cells, as well as disruption of cell division. (cdc.gov)

Despite the importance of the dermis in the structural and functional integrity of the skin, genetic analysis of dermal development in different parts of the embryo is incomplete. (biologists.com)
Our main model organism is the chick embryo , ideal for functional and translational studies on neural crest development. (lu.se)

Methods range from the basics of labeling cells to cutting-edge protocols for high-speed imaging, optical projection tomography, and digital scanned laser light-sheet fluorescence. (cshlpress.com)

Understanding gene function and regulation requires rigorous testing in live cells and organisms. (cshlpress.com)
Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)
Analysis of beta 2-microglobulin gene expression in the developing mouse embryo and placenta. (ulaval.ca)
Extracellular vaccinia virus formation and cell-to-cell virus transmission are prevented by deletion of the gene encoding the 37,000-Dalton outer envelope protein. (microbiologyresearch.org)

In vertebrates, this choice is mediated by the floor plate, and enables the embryo to develop successful left and right body halves with respect to nervous tissue. (wikipedia.org)

We studied the labelling of chick embryo tibia between Hamilton's stages 29 and 38 with a horseradish peroxydase labelled Ulex europaeus 1 lectin. (revmedvet.com)
Chick embryos at different stages. (lu.se)

1994. Inhibition of Na+-glucose cotransport in kidney cortical cells by cadmium and copper: Protection by zinc. (cdc.gov)

Netrins: Netrins are proteins expressed and secreted by cells of the floor plate. (wikipedia.org)
Moreover, Isolation and transfection of Netrin-1 and Netrin-2, two secreted proteins, into Cos cells has similar effects. (wikipedia.org)
α-crystallin is a member of the small heat shock protein family, which helps in maintaining and thickening of the cell wall and in providing stability to proteins that allow the bacteria to survive under harsher conditions 7 . (nature.com)
With electrical magnetic waves coupled with engineered ion channel proteins, we are able to impact specific biological cells remotely without affecting other biochemical environments," Liu said. (neurosciencenews.com)

However, Robo-3 (Rig-1) is upregulated during growth of commissural axons during migration toward the floor plate, which sequesters Robo-1/2 inside the cell within vesicles. (wikipedia.org)

A light and electron microscopic study of naturally occurring and induced cell loss during development. (karger.com)
The electron micrograph in the top panel shows two invaginating synapses between cone and horizontal cells in the outer p. (cellimagelibrary.org)

IEV is formed by wrapping of IMV with intracellular membranes, and is an intermediate between IMV and CEV/EEV that enables efficient virus dissemination to the cell surface on microtubules. (microbiologyresearch.org)

Programmed cell death (PCD) is a widespread phenomenon in the development of vertebrates. (karger.com)
In most cases, dying cells during development exhibit generalized morphological features typical of apoptosis. (karger.com)

These findings suggested the usefulness of them as an alternate source of cells for transplantation approaches to treat neurological disorders (Sakuragawa et al. (scielo.org.ar)
The findings, demonstrated in animal embryos, were published Oct. 10 in the journal Science Signaling . (neurosciencenews.com)

Two morphological types of cell death were found: apoptosis which was characterized by round or semilunar nuclear chromatin condensations, condensation and shrinkage of the cytoplasm and formation of apoptotic bodies, and cell death without the morphological features of apoptosis which was characterized by pyknotic nuclear chromatin condensations, vacuolated cytoplasm and the formation of numerous intercellular spaces. (karger.com)
We report the morphological differences of Müller cells in relation to their topography, using the Golgi method. (us.es)

J Cell Biol (1971) 51 (3): 636-642. (silverchair.com)

Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. (cshlpress.com)

Further studies will establish the nature of this novel factor which will enhance the application of this interesting cell type for neural transplantations. (scielo.org.ar)

It is imperative to understand the mechanism by which these cells exhibit neurotropism. (scielo.org.ar)

CEV induces the formation of actin tails that drive CEV particles away from the cell and is important for cell-to-cell spread. (microbiologyresearch.org)

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