• The functional specificity of three types of macrophage chemotactic factors (MCFs), -a, -b, and -c, from purified protein derivative of tuberculin (PPD)-induced delayed hypersensitivity reaction (DHR) skin sites on guinea pigs, was analyzed using macrophage cell line cells, M 1 , established from myeloid leukemia cells of a SL/Am strain mouse. (elsevierpure.com)
  • Upon differentiation to mature macrophage-like cells in conditioned medium (M + 1 cells), however, they migrated to MCF-a, -b, and -c and the experimental evidence points to the possibility that M + 1 cells are separated into subpopulations on the basis of their chemotactic response. (elsevierpure.com)
  • Both the endotoxin and extract increased macrophage chemotactic activity. (cdc.gov)
  • The diverse feature of the chemotaxis receptors and ligands provides the possibility to select chemotactic responder cells with a simple chemotaxis assay. (wikipedia.org)
  • Chemotaxis assays with chemotactic and non-chemotactic (Δ cheA ) L. agilis strains revealed that low pH, organic acids, and bile salts served as repellents. (nature.com)
  • Scholars@Duke publication: Human mononuclear leukocyte chemotaxis: a quantitative assay for humoral and cellular chemotactic factors. (duke.edu)
  • Cells were exposed to one of three chemotactic stimuli: platelet-activating factor (PAF), N-formyl-methionyl-leucyl-phenylalanine (FMLP), or leukotriene B4 (LTB4). (nih.gov)
  • We studied the changes in actin state and chemotactic peptide receptor expression in granulocytes from patients receiving different cytokines following high dose chemotherapy and autologous bone marrow transplantation (ABMT). (duke.edu)
  • These findings demonstrate that Ab binding of CD4 and CD8 interrupts a feed-forward circuit by suppressing T cell-produced cytokines needed for expression of chemotactic cues, leading to rapid T cell egress from the islets. (jci.org)
  • Chemokines," which take their name from chemotactic cytokines, are small secreted polypeptides that regulate movement of immune cells into tissues (Baggiolini et al. (justia.com)
  • MCP-4 shares the pyroglutamic acidproline NH2-terminal motif and 56-61% sequence identity with the three known monocyte chemotactic proteins and is 60% identical to eotaxin. (rupress.org)
  • The invention provides antibodies that bind to a plurality of β-chemokines, particularly monocyte chemotactic proteins MCP-1, MCP-2 and MCP-3. (justia.com)
  • Although highly homologous, both proteins are chemoattractants with distinct chemotactic activity for leukocyte subsets. (aai.org)
  • Chemokines of the alpha, beta-subclass inhibit human basophils' responsiveness to monocyte chemotactic and activating factor/monocyte chemoattractant protein-1. (musc.edu)
  • Description: A sandwich ELISA kit for detection of Monocyte Chemotactic Protein 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (scalegen.com)
  • Description: Quantitativesandwich ELISA kit for measuring Pig monocyte chemotactic protein 1, MCP1 in samples from serum, plasma. (scalegen.com)
  • Human neuroblastoma cells express alpha and beta platelet-derived growth factor receptors coupling with neurotrophic and chemotactic signaling. (jci.org)
  • Regulation of Monocyte Chemotactic Protein - 1 secretion by the Two-Pore-Domain Potassium (K2P) channel TREK- 1 in human alveolar epithelial cells. (mcp-1.com)
  • Scholars@Duke publication: Changes in actin state and chemotactic peptide receptor expression in granulocytes during cytokine administration after autologous bone marrow transplantation. (duke.edu)
  • Increased sensitivity to a C5a chemotactic gradient could make the ultrafine exposed macrophages more likely to be retained in the lungs, so allowing dose to accumulate. (hw.ac.uk)
  • Complement factor C5a exerts its effect through the activation of C5aR1, chemotactic receptor 1, and triggers the G protein-coupled signaling cascade. (drgpcr.com)
  • This assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 1 (MCP1). (uscnk.com)
  • No significant cross-reactivity or interference between Monocyte Chemotactic Protein 1 (MCP1) and analogues was observed. (uscnk.com)
  • Matrices listed below were spiked with certain level of recombinant Monocyte Chemotactic Protein 1 (MCP1) and the recovery rates were calculated by comparing the measured value to the expected amount of Monocyte Chemotactic Protein 1 (MCP1) in samples. (uscnk.com)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively. (uscnk.com)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate. (uscnk.com)
  • The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Monocyte Chemotactic Protein 1 (MCP1) and their serial dilutions. (uscnk.com)
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). (uscnk.com)
  • Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). (uscnk.com)
  • After TMB substrate solution is added, only those wells that contain Monocyte Chemotactic Protein 1 (MCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. (uscnk.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Monocyte Chemotactic Protein 1 (MCP1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (scalegen.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Monocyte Chemotactic Protein 1 (MCP1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (scalegen.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Rat Monocyte Chemotactic Protein 1 (MCP1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (scalegen.com)
  • Whereas the chemotactic peptide, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMet-Leu-Phe), induced NADPH-oxidase-catalyzed superoxide (O2-) formation in human neutrophils, purine and pyrimidine nucleotides per se did not stimulate NADPH oxidase but enhanced O2- formation induced by submaximally and maximally stimulatory concentrations of fMet-Leu-Phe up to fivefold. (uni-regensburg.de)
  • Although the basal level of F-actin was high following ABMT, granulocytes from all patients showed an additional increase in F-actin content after stimulation with either the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP) or phorbol myristate acetate (PMA). (duke.edu)
  • The chemotactic peptide receptor expression was significantly higher in patients treated with ABMT alone or ABMT plus G-CSF. (duke.edu)
  • Chandler, DE & Kazilek, CJ 1986, ' Chemotactic peptide-induced exocytosis in neutrophils: Granule fusion patterns depend on the source of messenger calcium ', Journal of Cell Science , vol. (elsevierpure.com)
  • Diagnosis, pathogenesis and outcome in leucocyte chemotactic factor-2 (ALECT2) amyloidosis. (emedinexus.com)
  • A new article published in Nephrology, Dialysis, Transplantation reported the first case series from a European Centre of prevalence, clinical presentation and diagnostic findings in leucocyte chemotactic factor-2 (ALECT2) amyloidosis and disclosed long-term patient and renal outcomes. (emedinexus.com)
  • CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. (watson-int.cn)
  • Term chemotactic selection is also used to design a technique which separates eukaryotic or prokaryotic cells upon their chemotactic responsiveness to selector ligands. (wikipedia.org)
  • Microfluidic devices fitted with "flowver" paper pumps generate steady, tunable gradients for extended observation of chemotactic cell migration. (ncsu.edu)
  • For both bacteria we recover and quantify the classical chemotactic strategy, where the tumble rate is smallest along the chemical gradient. (tu-berlin.de)
  • This chemotactic signaling pathway is relatively conserved across bacteria, whereas chemoeffectors and chemoreceptors vary among bacterial species/strains. (nature.com)
  • Comprehensive analysis of chemotactic activity of the eukaryotic protozoon Tetrahymena pyriformis and consensus sequences of appearance of amino acids in the primordial soup suggest that there was a good correlation between the chemotactic character of these relative simple organic molecules and their development on the Earth. (wikidoc.org)
  • In the 1960s and 1970s, the revolution of modern cell biology and biochemistry provided a series of novel techniques which became available to investigate the migratory responder cells and subcellular fractions responsible for chemotactic activity. (wikidoc.org)
  • abstract = "Studies showed that monocyte chemotactic protein-1 (MCP-1) concentrations are increased in obesity. (johnshopkins.edu)
  • Monocyte chemotactic protein 4 (MCP-4), a novel structural and functional analogue of MCP-3 and eotaxin. (rupress.org)
  • The physical constraints posed by hydrodynamics in the locomotion of microorganisms can combine with their chemotactic ability to significantly affect functions like colonization of nutrient sources. (purdue.edu)
  • Human MCP-1, MCP-2 and MCP-3 all have chemotactic activity for a variety of cell types, including T lymphocytes and monocytes (Van Coillie et al. (justia.com)
  • Here, we theoretically show that the stability of such chemotactic fronts to morphological perturbations is determined by limitations in the ability of individual cells to sense and thereby respond to the chemical gradient. (princeton.edu)
  • Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. (watson-int.cn)
  • By chemotactic selection we can determine whether a still not characterized molecule acts via the long- or the short-term receptor pathway. (wikipedia.org)
  • The terms on the right-hand side of Eq. ( 1 ) represent chemotactic movement and nonlinear diffusion, respectively. (nature.com)