Various catalytic reaction models have been proposed as the reaction mechanisms of glycosidases, but a reasonable and unitary model capable of interpreting both "inverting" and "retaining" glycosidase reactions remains to be established. (go.jp)
The drug moieties may affect cytotoxic and cytostatic mechanisms including tubulin binding, DNA binding, or topoisomerase inhibition. (justia.com)
Organic chemistry2
This reaction model, however, is considered to comprise some inconsistencies that cannot be neglected from the viewpoint of reactivity in organic chemistry. (go.jp)
Our research involves a combination of molecular biology, protein biochemistry, organic synthesis and structural studies and provides a strong training for students interested in understanding the organic chemistry of living systems and in pursuing careers in biotechnology, drug design or academia. (tamu.edu)
Mutations2
Protein biosynthesis has a key role in disease as changes and errors in this process, through underlying DNA mutations or protein misfolding, are often the underlying causes of a disease. (wikipedia.org)
During transcription, a section of DNA encoding a protein, known as a gene, is converted into a template molecule called messenger RNA (mRNA). (wikipedia.org)
Transcription occurs in the nucleus using DNA as a template to produce mRNA. (wikipedia.org)
The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast. (academicinfluence.com)
Gene1
The helicase disrupts the hydrogen bonds causing a region of DNA - corresponding to a gene - to unwind, separating the two DNA strands and exposing a series of bases. (wikipedia.org)
Glycosylase6
A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. (nih.gov)
It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40 degrees C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli. (nih.gov)
Molecular cloning, sequency, and expression of the heat-labile uracil-DNA glycosylase from a marine psychrophilic bacterium, strain BMTU3346. (nih.gov)
Purification and characterization of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua). (nih.gov)
Properties of a recombinant human uracil-DNA glycosylase from the UNG gene and evidence that UNG encodes the major uracil-DNA glycosylase. (nih.gov)
APOBEC3A preferentially deaminates cytosines to uracils in ssDNA, which are then converted to abasic sites by uracilDNA glycosylase. (nih.gov)
Double-Stranded1
Despite DNA being a double-stranded molecule, only one of the strands acts as a template for pre-mRNA synthesis - this strand is known as the template strand. (wikipedia.org)
Synthesis2
An enzyme that catalyzes the HYDROLYSIS of the N-glycosidic bond between sugar phosphate backbone and URACIL residue during DNA synthesis. (bvsalud.org)
Using Affymetrix GENECHIP arrays, specific hybridization can be achieved by direct synthesis of the DNA sequences of interest on arrays. (justia.com)
During transcription, a section of DNA encoding a protein, known as a gene, is converted into a template molecule called messenger RNA (mRNA). (wikipedia.org)
Initially, an enzyme known as a helicase acts on the molecule of DNA. (wikipedia.org)
Both DNA and RNA have intrinsic directionality, meaning there are two distinct ends of the molecule. (wikipedia.org)
This clonal amplification enables a single DNA molecule to be amplified on solid phase, which physically separates one molecule from another. (justia.com)
Bases1
During these reactions, separate labeling with 4 florophores or haptens, one for each of the 4 DNA bases, allows for the identification of the incorporated base at any given position. (justia.com)
Protein1
Protein biosynthesis has a key role in disease as changes and errors in this process, through underlying DNA mutations or protein misfolding, are often the underlying causes of a disease. (wikipedia.org)
Application1
However, the number of molecules hybridized to the probes is generally lower than the amount necessary for efficient application of some next generation sequencing chemistries. (justia.com)