Chem backbone peptide nucleic acids. Medical search. Frequent questions

Experimental methods of multi-dimensional NMR spectroscopy, dynamics and theoretical description of spin system evolution, multiple resonance NMR technique for structural studies of proteins and nucleic acids by NMR spectroscopy, contemporary methods of structural biology, studies of complex biomolecular systems. (muni.cz)
Rhombotin 1 (RBTN1 or TTG-1) and rhombotin-2 (RBTN2 or TTG-2) are proteins of about 160 amino acids whose genes are disrupted by chromosomal translocations in T-cell leukemia. (embl.de)
A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)
Their amino acid sequences suggest that they are mainly cytosolic or nuclear proteins partly associating with membranes (Talbot et al. (springer.com)
These groups are compatible with biological molecules, as they do not react with the functional groups found in nucleic acids, proteins and lipids. (biosearchtech.com)
Cysteine-Selective Modification of Peptides and Proteins via Desulfurative C-C Bond Formation CHEMISTRY-A EUROPEAN JOURNAL. (nottingham.ac.uk)

It has been used to label oligonucleotides with fluorescent dyes, sugars, peptides and other reporter groups, to cyclise DNA, to synthesise DNA catenanes, to join oligonucleotides to PNA, and to produce analogues of DNA with modified nucleobases and backbones. (ox.ac.uk)
Mesyl phosphoramidate backbone modified antisense oligonucleotides targeting miR-21 with enhanced in vivo therapeutic potency. (yale.edu)
The technique is widely used to label DNA, RNA and locked nucleic acid (LNA) oligonucleotides, as well as to cyclise DNA, join oligonucleotides to peptide nucleic acids (PNA), synthesise DNA catenanes and create analogues of DNA with modified nucleobases and backbones. (biosearchtech.com)

Addition of D -alanine to TAs reduces the negative charge of the cell envelope thereby preventing cationic antimicrobial peptides (CAMPs) from reaching their target of action on the bacterial surface. (frontiersin.org)
I have been intrigued by the activity of antimicrobial peptides for a long time, due to my interest in biological membranes. (rsc.org)
Antimicrobial peptides have long been hailed as a solution to the antibiotic resistance crisis, because they target the bacterial membrane and can cause quick bactericidal activity in bacterial cells. (rsc.org)
Most antimicrobial peptides are cationic amphiphilic compounds that function by binding to the negatively charged lipids of bacterial membranes, followed by membrane disruption. (rsc.org)
While binding to the lipid headgroup is detrimental for anion transport, I hypothesized that binding to the headgroup can provide membrane selectivity (e.g., bacterial membranes over human membranes) and can induce membrane perturbations similar to those observed for antimicrobial peptides. (rsc.org)
We show here that the cementoin domain adopts an α-helical conformation both by circular dichroism and nuclear magnetic resonance analyses in the presence of membrane mimetics, a characteristic shared with a large number of linear polycationic antimicrobial peptides. (biomedcentral.com)
The N-terminal cementoin domain adopts α-helical secondary structures in a membrane mimetic environment, which is common in antimicrobial peptides. (biomedcentral.com)
However, unlike numerous linear polycationic antimicrobial peptides, membrane disruption does not appear to be the main function of either cementoin, elafin or full-length pre-elafin/trappin-2 against P. aeruginosa . (biomedcentral.com)
In the past decade, gene-encoded short positively charged peptides, collectively known as antimicrobial peptides (AMP), have attracted much attention because of their broad antimicrobial activities and their potential use as therapeutics [ 11 - 18 ]. (biomedcentral.com)

Direct the discovery of novel biologics with a comprehensive platform for protein and nucleic acid engineering and reduce experimental burden through computational prediction. (schrodinger.com)
Highly divergent hypotheses have been put forward regarding the makeup of the prions, including that they consist of nucleic acid only or protein only, are lacking both protein and nucleic acid, or are a polysaccharide. (medscape.com)

2021 ). The human DBNDD1 isoforms 1 (UniProtKB: Q9H9R9-1) and 2 (UniProtKB: Q9H9R9-2) differ only in the N-terminal region where 20 amino acids are additional in isoform 2. (springer.com)
Nucleic Acids Research (Database Issue) 49, D86-D91 (2021). (sparks-lab.org)
Nucleic Acids Research 49, D298-D308 (2021). (sparks-lab.org)

9. Synthesis of protected pseudopeptides from dicarboxylic amino acids by Mitsunobu condensation / N. P. Boyarskaya, D. I. Prokhorov, Yu. (finechem-mirea.ru)
Abiogenesis Glycol nucleic acid Oligonucleotide synthesis Peptide nucleic acid Synthetic biology Xeno nucleic acid Xenobiology Schöning, K. U. et al. (wikipedia.org)
The Core director Dr. Remmer has 24 years of experience directing a Proteomics & Peptide Synthesis Core. (umich.edu)
2006. Backbone Dynamics Determined by Electron Paramagnetic Resonance to Optimize Solid Phase Peptide Synthesis of TOAC-labeled Phospholamban. (umich.edu)
Discovery of molecules with interesting biological activity using screening approaches such as combinatorial chemistry and multiple organic synthesis, and rational approaches starting from the natural substrates, especially from peptide sequences. (biu.ac.il)
During her M.S., she focused on the development of fluorescent amino acids and peptide synthesis. (rsc.org)

Because of its antigene, antisense, anti-miR, and the scope to be labeled with various fluorophores, PNA finds application in gene expression modulation in-vivo , and development of nucleic acid-based biosensors which are useful in biotechnology researches and clinical diagnostics. (creative-peptides.com)
1. James, W. Towards gene-inhibition therapy: a review of progress and prospects in the field of antiviral antisense nucleic acids and ribozymes / W. James // Antiviral Chem .and Chemotherapy. (finechem-mirea.ru)

2002) J Biol Chem, 277: 27872-27879), and inhibition of the wnt-signaling pathway (Hanai et al. (justia.com)

Finally, the same tools used to create these models can be readily applied to other types of backbones and nucleobases for endless research and education possibilities. (allenpress.com)
However, these issues can be overcome by the chemical flexibility of PNA that allows for the insertion of modifications on its backbone and nucleobases. (creative-peptides.com)

Click DNA labelling is commonly used to post-synthetically conjugate alkyne-labelled DNA to azide-functionalised reporter groups such as fluorescent dyes, sugars and peptides. (biosearchtech.com)
High selectivity for the determination of mandelic acid was achieved compared to metal ions, amino acids, and sugars that commonly coexist with it. (bvsalud.org)

Backbone modifications for enhanced cellular uptake: For enhanced cellular delivery, PNA has been conjugated with cell penetrating peptide (CPP), cationic peptides, and basic amino acid stretches such as Lysine and Arginine. (creative-peptides.com)

The results of investigation are more effective steps for allyl ester protective group incorporation and coupling of thymin-1-yl acetic acid with pseudopeptide intermediate. (finechem-mirea.ru)

Chemical shift changes upon the addition of single-stranded RNAs (ssRNAs) identified a group of residues that form a positively charged patch on the protein surface as the binding site responsible for the previously reported affinity for nucleic acids. (pdfroom.com)

Recently PNAs, because of their excellent affinity to DNA/RNA and the scope to be modified as per the application, have attracted a wide range of researchers for the development of new PNA molecules by a simple backbone or nucleobase modifications. (creative-peptides.com)
Backbone modifications of PNA (B = nucleobase) (Perera JDR, et al . (creative-peptides.com)

Building on previous research, representative nucleic acid duplexes were split into logical monomer segments, and atomic coordinates were used to create solid models for 3D printing. (allenpress.com)

Such reactions employ agents that act by hydrolysis of peptide bonds and the specificity of the cleavage agent is determined by the identity of the amino acid residue at or near the peptide bond which is cleaved. (justia.com)

Backbone modification and Hybridization properties: Hence the enhancement of binding affinity and sequence specificity of PNA for DNA and RNA complement via chemical modification of PNA backbone is highly desirable and will have significant contribution for potential application in therapeutics and diagnostics. (creative-peptides.com)

Dynamic and flexible nucleic acid models can provide current and future scientists with physical intuition for the structure of DNA and the ways that DNA and its synthetic mimics can be used to build self-assembling structures and advanced nanomachines. (allenpress.com)
Efforts to mimic nature typically utilize amino acids and amino acid derivatives to form peptidomimetics having amide bonds.Presented here is a new class of synthetic sequence-defined polymers which do not use peptide bonds. (pnnl.gov)

Peptide nucleic acid (PNA) is an artificial oligonucleotide mimetic with a peptidic backbone in lieu of a phosphoribosyl backbone. (creative-peptides.com)
Chemical etiology of nucleic acid structure: the a-threofuranosyl-(3'-->2') oligonucleotide system. (wikipedia.org)
Methyl phosphonoamidites are deoxynucleoside amidites modified such that, when incorporated into an oligonucleotide, that base position will have a (electrically neutral) methyl phosphonate backbone linkage instead of the standard (negatively charged) phosphodiester linkage. (genelink.com)

Peptide Solubility Limits: Backbone and Side-Chain Interactions. (utmb.edu)
Impact of nucleic acid self-alignment in a strong magnetic field on the interpretation of indirect spin-spin interactions. (muni.cz)
Design of peptide-membrane interactions to modulate single-file water transport through modified gramicidin channels. (irbbarcelona.org)
Z. Cao, L. Liu, G. Hu, Y. Bian, H. Li, J. Wang, and Y. Zhou, "Interplay of hydrophobic and hydrophilic interactions in sequence-dependent cell penetration of spontaneous membrane-translocating peptides revealed by bias-exchange metadynamics simulations. (sparks-lab.org)

This modified backbone makes TNA, unlike DNA and RNA, completely refractory to nuclease digestion, making it a promising nucleic acid analog for therapeutic and diagnostic applications. (wikipedia.org)
The high biological stability of TNA relative to other nucleic acid systems that are capable of undergoing Darwinian evolution, suggests that TNA is a strong candidate for the development of next-generation therapeutic aptamers. (wikipedia.org)
I have designed and led several national and international research projects focused on deciphering enzymatic reaction mechanisms and engineering nucleic acids for therapeutic purposes using a range of HPC chemistry technologies and tools. (vgenna.com)

Amino-functional silica-coated N-doped carbon dots (NH2-SiO2-CDs) were covalently modified by l-tryptophan (chiral selector) by producing an amide bond between carboxyl groups of L-try and amino groups of NH2-SiO2-CDs to develop a novel high throughput chiral nanoprobes (L-try-CONH-SiO2-CDs) for highly sensitive and enantioselective quantification of S-/R-mandelic acid (S-/R-Man). (bvsalud.org)

Chiral PNA Backbone: Chiral PNA backbones with modifications at the α-, β-, and γ-positions have been developed, each possessing unique oligomer binding and conformational properties. (creative-peptides.com)
4. Peptide nucleic acids with a structurally biased backbone: effects of conformational constraints and stereochemistry / R. Corradini [et al. (finechem-mirea.ru)

Backbone FC-H···O hydrogen bonds in 2'F-substituted nucleic acids. (irbbarcelona.org)
The 2H relaxation data also provided information about the orientational order and internal motion of OD and ND bonds in side-chains and surface peptides. (lu.se)

DNA and RNA have their phosphate backbones attached to the 5' carbon of the deoxyribose or ribose sugar ring, respectively. (wikipedia.org)
TNA, on the other hand, has it's phosphate backbone directly attached to the 3' carbon in the ring, since it does not have a 5' carbon. (wikipedia.org)
The cell wall of gram-positive bacteria contains highly charged anionic polymers called teichoic acids (TAs) that consist of alditol phosphate repeats. (frontiersin.org)
PMO backbones are derived from RNA, wherein the five-membered ribosyl ring has been replaced with a six-membered morpholino ring, and phosphate linkages have been replaced with uncharged phosphorodiamidates (Fig. 1a ). (nature.com)

A common feature ascribed to AMP is their ability to interact with the negatively charged bacterial membranes and polyanionic cell surface (lipopolysaccharide (LPS) of Gram-negative and lipoteichoic acid of Gram-positive bacteria). (biomedcentral.com)

This sequential reactivity provides advancement over prior methods used to form conventional sequence-defined peptide polymers because it provides completely new polymeric architectures, as compared to conventional peptide, peptoid, or nucleic acid polymers. (pnnl.gov)
Their binding properties depend on the amino acid sequence of the finger domains and of the linker between fingers, as well as on the higher-order structures and the number of fingers. (embl.de)
vi)a nucleic acid sequence according to any of (i) and (ii) which is degenerate as a result of the genetic code. (allindianpatents.com)
In contrast, isoform 3 (UniProtKB: Q9H9R9-3) carries a 100 amino acids long N-terminal sequence extension. (springer.com)
E2EDNA 2.0 takes in a DNA aptamer sequence in FASTA format, and optionally a short peptide or other small molecule, and returns details of the aptamer structure and binding behaviour. (pythonawesome.com)

J Biol Chem. (le.ac.uk)
Curr Opin Chem Biol. (chicagobiomedicalconsortium.org)
2003) J Biol Chem, 278: 37895-37901), blockage of VEGF signaling (Hajitou et al. (justia.com)

Despite the advantageous features of classic PNA, its low water solubility, poor cellular uptake, propensity to self-aggregate, and orientational ambiguity in recognizing nucleic acid targets, limits the effectiveness of PNA in its applications. (creative-peptides.com)

It has also been proposed to employ fusion partners as affinity peptides. (justia.com)

10. McKennon, M. A convenient reduction of amino acids and their derivatives / M. McKennon, A. Meyers // J. Org. (finechem-mirea.ru)

Members of the institute have published in a wide range of peer-reviewed journals, including Nature, Molecular Cell, Angewandte Chemie, JACS, Science Advances, Proceedings of the National Academy of Sciences USA, Nucleic Acids Research, Chemical Science and many others. (le.ac.uk)
This methodology facilitates the isolation and recovery of the fusion peptide from the host cells by exploiting the physico-chemical properties of the fusion partner. (justia.com)
Time averaging of NMR chemical shifts in the MLF peptide in the solid state. (irbbarcelona.org)
Backbone 1H and 15N Chemical Shift Assignments for Hen Egg White Lysozyme mutant W28G. (bmrb.io)

This pD dependence is due to labile protein deuterons in acidic side-chains and surface peptide groups, which exchange rapidly with water deuterons. (lu.se)

Threose nucleic acid (TNA) is an artificial genetic polymer in which the natural five-carbon ribose sugar found in RNA has been replaced by an unnatural four-carbon threose sugar. (wikipedia.org)

As more research labs and classrooms dive into the field of structural nucleic acid nanotechnology, students and researchers need access to interactive, dynamic, handheld models. (allenpress.com)

Pol II, assisted by the neurodegeneration-associated enzyme senataxin, generates a shield comprising triplex nucleic acid structures known as R-loops at intergenic spacers flanking nucleolar rRNA genes. (nature.com)

The advent of click chemistry has led to an influx of new ideas in the nucleic acids field. (ox.ac.uk)
Several studies have demonstrated the use of click chemistry in a variety of nucleic acid assemblies, including ligation, circular constructs and catenanes. (biosearchtech.com)

Detritylation frees the 3′-terminal amine with the conjugate acid of a non-nucleophilic heterocycle, typically 4-cyanopyridine in dichloromethane-trifluoroethanol (DCM/TFE) mixtures. (nature.com)
Typically, azide-functionalised DNA, PNA or modified nucleic acids are linked to alkyne end-functional diblock copolymers. (biosearchtech.com)

A randomly folded peptide nucleic acid has been transformed into a right-handed helix by simple modification at the γ-position of the backbone. (creative-peptides.com)

Valproic acid treatment rescues injured tissues after traumatic brain injury. (umich.edu)

2018. Rapid valproic-acid induced modulation of the traumatic proteome in a porcine model of traumatic brain injury and hemorrhagic shock. (umich.edu)

Avian and human influenza A viruses prefer binding to sialic acids linked to a penultimate galactose by an α2-3 or α2-6 linkage, respectively ( 18 ). (cdc.gov)

The aim of the present study was to determine the secondary structures of the cementoin domain and to characterize the antibacterial properties of these peptides against P. aeruginosa . (biomedcentral.com)

J. Singh, K. Paliwal, J. Singh, Y. Zhou, "RNA Backbone Torsion and Pseudotorsion Angle Prediction using Dilated Convolutional Neural Networks. (sparks-lab.org)

Here, we present the backbone and side chain nuclear magnetic resonance assignments for the human DBNDD1 protein. (springer.com)

Provided herein are anti-angiogenic comprising the N-terminal end of endostatin, nucleic acids encoding the same, pharmaceutical preparations comprising an effective amount of the peptide and nucleic acids and use of the pharmaceuticals in treating or preventing diseases or conditions associated with undesirable angiogenesis. (justia.com)

Molecular basis of substrate-induced permeation by an amino acid antiporter. (irbbarcelona.org)

The dlt operon of Gram-positive bacteria is required for the incorporation of D -alanine esters into cell wall-associated teichoic acids (TAs). (frontiersin.org)
one way that bacteria diversify siderophore structure is by incorporating different polyamine backbones while maintaining the catechol moieties. (biomedcentral.com)

As such, it combines the properties of both peptides and nucleic acids. (creative-peptides.com)

This hypothesis suggests that prions contain no nucleic acid and are referred to as PrP Sc . (medscape.com)

Anatomy1

Diseases2

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment7

Phenomena and Processes11

Information Science3

Health Care1

Proteins6

Oligonucleotides3

Antimicrobial peptides9

Protein and nucleic acid2

20213

Synthesis6

Antisense2

20021

Nucleobases2

Sugars2

Cationic1

Pseudopeptide1

Residues1

Nucleobase2

Duplexes1

Hydrolysis1

Specificity1

Synthetic2

Oligonucleotide3

Interactions4

Therapeutic3

Amide1

Conformational2

Bonds2

Phosphate4

Lipoteichoic acid1

Sequence5

Biol3

Limits1

Affinity1

Derivatives1

Chemical4

Acidic1

Artificial1

Structural1

Enzyme1

Chemistry2

Typically2

Modification1

Tissues1

20181

Viruses1

Structures1

Prediction1

Resonance1

Pharmaceuticals1

Molecular1

Bacteria2

Properties1

Suggests1