• This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. (utsouthwestern.edu)
  • hTopBP1 and hMYH were involved in ATR-mediated Chk1 activation, moreover, both of them were associated with ATR and hRad9 which known as checkpoint-involved proteins. (biomedcentral.com)
  • In addition, we investigated several cell cycle-related proteins and found that co-knockdown of hTopBP1 and hMYH significantly diminished cell cycle arrest due to compromised checkpoint kinase 1 (Chk1) activation. (biomedcentral.com)
  • Small molecule inhibitors designed to target the DNA damage sensors, such as inhibitors of ataxia telangiectasia-mutated (ATM), ATR, CHK1 and WEE1, impair smooth cell cycle modulation and disrupt efficient DNA repair, or a combination of the above, have demonstrated interesting monotherapy and combinatorial activity, including the potential to reverse drug resistance and have entered developmental pipelines. (bmj.com)
  • HUS1 has been shown to interact with: HDAC1, PCNA, RAD1 homolog RAD17, and RAD9A. (wikipedia.org)
  • In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. (wikipedia.org)
  • Moreover, we observed that hMYH was essential for the accumulation of hTopBP1 on damaged DNA, where hTopBP1 interacts with hRad9, a component of the Rad9-Hus1-Rad1 complex. (biomedcentral.com)
  • Then hTopBP1 interacts with ATR-ATRIP through its ATR-activating domain (AD) and stimulates ATR kinase activity [ 8 , 9 ]. (biomedcentral.com)
  • Maintaining genomic integrity is of utmost importance to eukaryotic cells, which have evolved sophisticated mechanisms to ensure speed, accuracy, and an adequate pool of nucleotide and replication factors as well as high-fidelity repair pathways to correct errors occurring during DNA replication. (bmj.com)
  • Please see the Entrez Gene record to access additional publications. (avivasysbio.com)
  • Checkpoint protein HUS1 is a protein that in humans is encoded by the HUS1 gene. (wikipedia.org)
  • The cellular response to DNA damage involves an intricate network of enzymes responsible for sensing, signaling, and repairing damaged DNA, as well as the regulation of cell cycle checkpoints that collectively maintain genomic integrity 2 . (nature.com)
  • Any obstacles encountered by cells in this process can lead to 'replicative stress' ( Figure 1 ), 1 which may be overcome by replicative stress response proteins, but deficiencies in this response result in accumulated errors in DNA replication and loss of genomic integrity, which lead to cell death. (bmj.com)
  • These are extremely hazardous for a cell, because if left unrepaired, DSBs can have pathological consequences, such as cell death, or drive cells to genomic instability and tumor development. (nature.com)
  • Conversely the dynamin-related protein 1 (Drp1/DNM1) is definitely a cytosolic protein recruitment of which to the OMM from the anchored fission 1 protein (Fis1p/FIS1) adaptor initiates and settings the fission and distribution of mitochondria in cells [19]. (tech-strategy.org)
  • The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. (wikipedia.org)
  • Human DNA topoisomerase II-binding protein 1 (hTopBP1) plays an important role in DNA replication and the DNA damage checkpoint pathway. (biomedcentral.com)
  • Thus, hTopBP1 constitutes an important part of the ATR signaling pathway and acts as a molecular bridge that associates the independently recruited 9-1-1 and ATR-ATRIP complexes, thereby leading to checkpoint activation [ 4 ]. (biomedcentral.com)
  • The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway evaluation suggested that furthermore to Wnt and changing growth aspect (TGF) signaling pathways, that have been reported in response to follicle formation broadly, another mixed band of signaling pathways that regulate lipid synthesis and fat burning capacity, like the phosphatidylinositol signaling program and glycerolipid signaling and fat burning capacity, are Gemzar biological activity in charge of follicle formation also. (clinical-research-informatics.com)
  • It was also recently shown that PARP-1 is a sensor of unligated Okazaki fragments during DNA replication 16 and cells deficient in ribonucleotide excision repair are sensitized to PARP inhibition 17 . (nature.com)
  • Finally we shown that CTMP delayed PKB/Akt phosphorylation following cell death induction suggesting that CTMP regulates apoptosis via inhibition of PKB/Akt. (tech-strategy.org)
  • Each origin is initiated by a combination of regulatory proteins that prepare the chromatin for replication before synthesis (S)-phase entry. (bmj.com)
  • This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. (avivasysbio.com)
  • The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. (wikipedia.org)
  • Research with yeast resulted in the identification from the conserved mammalian "mitochondria-shaping" protein. (tech-strategy.org)
  • During ATR signaling in response to DNA damage, Rad17 forms a complex with 9-1-1 and loads onto stalled replication forks [ 4 - 9 ]. (biomedcentral.com)
  • Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. (avivasysbio.com)
  • An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. (utsouthwestern.edu)
  • Through its interactions with other proteins via its BRCT domains, hTopBP1 performs diverse functions [ 1 ]. (biomedcentral.com)
  • One of the oxidative DNA lesions frequently generated upon exposure of cells to reactive oxygen species (ROS) is 7,8-dihydro-8-oxoguanine (8-oxoG) [ 13 ]. (biomedcentral.com)
  • In the presence of errors or damage during DNA replication, cell cycle checkpoint nodes and repair machinery work in concert to retard cell cycle progression until sufficient repair has been achieved. (bmj.com)
  • outcomes support the relevance of the results as mitochondria from livers of adult CTMP knockout mice experienced a similar phenotype to cells NKP608 depleted of CTMP. (tech-strategy.org)
  • In flies, worms and yeast, the 9-1-1 complex is necessary for meiotic checkpoint function and efficient meiotic recombination. (wikipedia.org)
  • The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast. (academicinfluence.com)
  • a connection between mitochondrial function and dynamics in the legislation of fat burning capacity cell loss of life neurotransmission cell routine control and advancement [15]. (tech-strategy.org)
  • Essential gene? (utsouthwestern.edu)
  • Profusion protein like the dynamin-related proteins mitofusins 1 and 2 (Mfn1 and Mfn2) are essential the different parts of the external mitochondrial membrane (OMM) essential to mitochondria tethering and fusion [16] [17]. (tech-strategy.org)
  • Poly(ADP-ribose) polymerase-1 (PARP-1) is an abundant and ubiquitous nuclear protein that uses NAD + to synthesize a multibranched polyanion composed of ADP-ribose moieties, giving rise to poly(ADP-ribose) (PAR), onto itself or a variety of target proteins. (nature.com)
  • XLS 97 kb) 12861_2017_153_MOESM8_ESM.xls (98K) GUID:?B2FE5241-0444-4D48-99D4-D5E55E4120EF Extra file 9: Desk S7: Target genes of the very best portrayed 18 miRNAs. (clinical-research-informatics.com)
  • This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. (avivasysbio.com)
  • The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. (utsouthwestern.edu)
  • PARP-1 is the best-characterized member of the diphtheria toxin-like ADP-ribosyl transferases (ARTDs) family of proteins. (nature.com)
  • Strategies that increase replicative stress while lowering cell cycle checkpoint thresholds may allow unrepaired DNA damage to be inappropriately carried forward in replicating cells, leading to mitotic catastrophe and cell death. (bmj.com)
  • Several PAR-binding modules orchestrate the relocation of DDR-associated factors in addition to the accumulation of intrinsically disordered proteins through an intracellular liquid demixing mechanism 11 , 12 . (nature.com)
  • Previously NKP608 we recognized the Carboxy-Terminal Modulator Protein (CTMP) inside a two-hybrid search for PKB/Akt binding partners [20]. (tech-strategy.org)