Characterize protein protein modification translational. Medical search. Frequent questions

Post-translational modification mechanisms may therefore play a significant role in the regulation of these pathways. (nih.gov)
Ubiquitylation is a reversible post-translational modification whose most well-known and best characterized function is tagging proteins for proteolytic degradation. (dana-farber.org)
This framework will lead to elucidate specific mechanisms of regulation for each type of post-translational modification and be able to make functional prediction the molecules participating by finding similar motifs in uncharacterized events. (europa.eu)
The majority of biological drugs are proteins, and most proteins undergo some form of post-translational modification (PTMs) after being translated from an mRNA molecule. (technologynetworks.com)
One type of post-translational modification involved in protein stability is glycosylation. (biorxiv.org)
Second, the new peptides do not contain disulfide bonds, a post-translational modification commonly encountered in animal toxins. (springer.com)
Tyrosine sulfation is an important post-translational modification that enhances the inhibitory activity of hirudin. (ccspublishing.org.cn)
Additionally, post-translational modification events like phosphorylation or others, caused by different cellular challenges (e.g. oxidative stress damage/ protection), are leading to a peculiar regulation of the activities of existing proteins inside the cell. (uni-greifswald.de)
The conformation of chromatin is profoundly influenced by the post-translational modification of the histone proteins. (promegaconnections.com)
They contain heme covalently bound to protein in a highly conserved, highly stereospecific post-translational modification. (ox.ac.uk)
Regarding size, some antigenically active recombinant polypeptides were as calculated, whereas the other ones apparently resulted from post translational modification. (agrobiology.ru)
More recently, the concept of lysosomal storage disease has been expanded to include deficiencies or defects in proteins necessary for the normal post-translational modification of lysosomal enzymes (which themselves are often glycoproteins), activator proteins, or proteins important for proper intracellular trafficking between the lysosome and other intracellular compartments. (medscape.com)

The research in Dr. Wan's lab is focused on protein post-translational modifications, including ubiquitination, acetylation, methylation, and phosphorylation. (postdocjobs.com)
In addition, due to their importance in cellular processes, means for the analyses of various post-translational modifications (PTM), including phosphorylation, glycosylation, ubiquitination and SUMOylation are provided. (uni-ulm.de)
Major research interests are cancer proteomics, focusing on the secretome, tumoral dynamics, quantitative proteomics, protein phosphorylation, and the stoichiometry of ubiquitination and acetylation. (hupo.org)
Phosphorylation of the eIF2alpha subunit is a common mechanism for down-regulating protein synthesis under stress conditions. (nih.gov)
Phosphorylation of eIF2α on Ser51 inhibits 5' cap-dependent mRNA translation, resulting in the global suppression of protein synthesis to facilitate adaptation to a variety of stresses linked to protein synthesis, including proteotoxic stress, viral replication, heme depletion and amino acid withdrawal [ 2 ]. (biomedcentral.com)

This project proposes building an in silico model of the cell including all these molecules and their interactions by adding several until now unconnected layers of information to address the topological characterization and comparison of several types of protein post-translational modifications, the major mechanism by which protein function is regulated in eukaryotes. (europa.eu)
In this webinar brought to you by MilliporeSigma, explore how automating 2D electrophoresis brings increased resolution to protein detection and characterization. (the-scientist.com)
The molecular approach of using Geneious Prime software and protein prediction data base for characterization of bacteriocin is novel and predicts " KSSAYSLQMGATAIKQVKKLFKKWGW " as peptide responsible for antimicrobial activity. (researchsquare.com)
Use of bioinformatics for functional characterization of genes and proteins. (liu.se)

I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
I further investigated membrane proteins and the targeting of tail-anchored proteins at Sheffield Hallam University. (brookes.ac.uk)
Novel GHz-class NMR technology enables advanced research into the structural basis for affinity and specificity of protein-ligand interactions, including a better understanding of structural features of cell membrane proteins, and the molecular mechanisms involved in protein folding and aggregation. (bruker.com)
Moreover, lateral motility of membrane proteins was analyzed by calculating diffusion coefficients and kymograph analysis, which represented an alternative method for examining protein motility. (biomedcentral.com)
They have been defined as monotropic integral membrane proteins located primarily in the endoplasmic reticulum (COX-1) and the perinuclear envelope (COX-2). (medscape.com)

The two major direct methods of protein sequencing are mass spectrometry and Edman degradation using a protein sequenator (sequencer). (wikipedia.org)
Mass spectrometry methods are now the most widely used for protein sequencing and identification but Edman degradation remains a valuable tool for characterizing a protein's N-terminus. (wikipedia.org)
Examples of modification by N-acetylation, adenylylation and proteolytic processing were characterized using mass spectrometry. (nih.gov)
Mariana Torrente received dual bachelor degrees in Chemistry and Biochemistry from the Florida Institute of Technology in 2005 and her Ph.D. in Chemistry in 2010 from Princeton University where she comprehensively cataloged chromatin proteins -particularly histones- during biologically significant processes using high-end mass spectrometry proteomics. (cuny.edu)
Mass spectrometry, mutagenesis, and functional assays investigate the impact of post-translational modifications on IL18RAP's conformation and function. (creativebiomart.net)

2-DE offers fine-scale resolution of thousands of proteins and, unlike traditional SDS-PAGE, allows researchers to distinguish and characterize different protein isoforms and post-translational modifications (PTMs). (the-scientist.com)
The growing market for biopharmaceuticals 1 brings new challenges for drug developers and manufacturers in assuring product quality and safety, and much of this can be attributed to product variability caused by post-translational modifications (PTMs). (technologynetworks.com)
The most common PTMs include glycosylation, oxidation and deamidation, but there are many different types of PTM and they can occur at numerous sites within a protein affecting biological activity, half-life and immunogenicity. (technologynetworks.com)
Different PTMs can affect different functional attributes of the drugs, depending on the mechanism of action," says Dr John Schiel, Research Chemist at the US National Institute of Standards and Technology (NIST) who researches new methods for characterizing PTMs in antibodies. (technologynetworks.com)
A toolbox of around 30 different analytical assays is currently used to characterize biopharmaceuticals, says Schiel, and some of those inform on PTMs. (technologynetworks.com)
Another characteristic of animal toxins is the remarkable number and variety of post-translational modifications (PTMs). (springer.com)
More unusual PTMs have also been characterized, especially within the cone snail family. (springer.com)
Contains utilities for the analysis of post-translational modifications (PTMs) in proteins, with particular emphasis on the sulfoxidation of methionine residues. (cran.csiro.au)

Also observed was that acetylation of histone tails reduces the association with both p66-proteins in vitro. (promegaconnections.com)

What experimental methods are used to elucidate the effects of post-translational modifications, such as glycosylation, on IL18RAP's structure and function? (creativebiomart.net)

We are also interested in developing small molecules that target protein-protein interactions in the ubiquitin system. (dana-farber.org)
The study of the composition of such a complex network including not only the description of their elements but also the structure of their relationships, i.e. its topology, was previously used to extract important elements (e.g. hubs proteins) and to find recurrent circuits that characterize regulatory events (e.g. feedforward loops) using mainly protein-protein interactions networks. (europa.eu)
I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
It is hypothesized that PCAIs work as anticancer agents by disrupting polyisoprenylation-dependent functional interactions of the G-Proteins. (oncotarget.com)
How are co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and biophysical assays applied to characterize the interactions between IL18RAP, IL18, and IL18R1? (creativebiomart.net)
Co-immunoprecipitation assays, FRET studies, and biophysical techniques characterize IL18RAP's interactions with IL18 and IL18R1 partners. (creativebiomart.net)
As a consequence, other IxI/V containing proteins can bind more to HSPB1, explaining the altered protein-protein interactions. (uantwerpen.be)
The PM provides an environment in which macromolecules interact efficiently, including the clustering of proteins in oligomeric complexes via protein-protein or protein-lipid interactions, the docking and anchoring of protein complexes for regulatory reactions and other precisely orchestrated processes [ 1 ]. (biomedcentral.com)
Protein-water interactions therefore play an essential role in (MRD) of the quadrupolar 2H and 17O nuclei in the water the folding, stability, dynamics, and function of proteins. (lu.se)
The first-order effect of the bulk solvent method can separately observe the hydration layer, because fast on the protein is often described by solvent-averaged potentials, water exchange between hydration sites and bulk solvent makes as in the dielectric screening of Coulomb interactions. (lu.se)

In a comprehensive biochemical, molecular and genomics study, they have provided evidence that the methylation of BRD4 inhibits the expression of genes which are involved in translation and abolish protein synthesis in cells. (indiaeducationdiary.in)
Un-balanced gene expression involved in protein synthesis may lead to increased proliferation and transformation which can subsequently result in the initiation and progression of cancer. (indiaeducationdiary.in)
All organisms are characterized by highly dynamic protein synthesis and degradation processes. (uni-greifswald.de)
We study the mechanism and regulation of protein synthesis, focusing on GTPases and protein kinases that control this fundamental cellular process. (nih.gov)
The first step of protein synthesis is binding of the initiator Met-tRNA to the small ribosomal subunit by the factor eIF2, which is composed of three subunits. (nih.gov)
While protein synthesis plays a critical role in learning and memory in model systems, human intellectual disability syndromes have not been directly associated with alterations in protein synthesis. (nih.gov)
The eIF2α kinases phosphorylate Ser51 of eIF2α which leads to suppression of global protein synthesis but selective enhancement of translation of some mRNAs, such as that encoding ATF4. (biomedcentral.com)

Products from 770 different genes were identified, and the types of proteins represented are discussed in terms of their annotated functional classes. (nih.gov)
Bruker's GHz-class NMR technology enables novel research in functional structural biology of proteins and protein complexes like intrinsically disordered proteins (IDPs). (bruker.com)
These peculiar properties confer them completely different functional advantages, compared with proteins that have a well-defined 3D structure. (bruker.com)

However, its critical role in protein activation/inactivation, localization, signal transduction and lysosomal and autophagic degradation among other cellular processes is becoming increasingly appreciated. (dana-farber.org)
5 4 Succinate functions as a competitive inhibitor for prolyl hydroxylase domain (PHD) proteins that are central to degradation of hypoxia-inducible factor (HIF)-1α subunit. (haematologica.org)

They will highlight how automated 2-DE streamlines a number of research applications, including the detection of residual host cell proteins (HCPs) in quality control and drug validation. (the-scientist.com)
This webinar will discuss several workflows for characterizing AAV CQAs, including product identity, post-translational modifications, and host cell proteins. (chromacademy.com)

Here, we aimed to spatially characterise the human Parkinsonian nigro-striatal N- glycome, and related transcriptome/proteome, and its correlation with endoplasmic reticulum stress and unfolded protein response (UPR), providing a comprehensive characterisation of the PD molecular signature. (biorxiv.org)

This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. (enzolifesciences.com)
In genetic and biochemical studies, we found that the mutation disrupts eIF2 complex integrity, impairs general translation, alters translational control of mRNAs encoding key regulatory proteins, and reduces the fidelity of translation start codon selection. (nih.gov)
The additional information generated by whole genome studies goes far beyond that derived by characterizing in isolation more genes and gene products, because analysis of the whole genome allows complete regulatory networks to be identified and characterized. (cdc.gov)

It is the most cost effective method to determine the absolute protein concentration for several hundreds of proteins and their isoforms in complex proteomes to date. (uni-greifswald.de)

The lab uses a wide range of approaches in our projects: molecular biology, protein biochemistry, in vitro cell culture models, xenograft and syngeneic mouse models, genetically engineered mouse models, various CRISPR-based techniques, and genomic/transcriptomic/proteomic omics techniques. (postdocjobs.com)
However, a number possible techniques and our experience can improve the outcome, and in vitro refolding is often successful to prepare active proteins. (creativelab.hu)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

One such modification is methylation, which refers to the addition of a chemical moiety (a methyl group) to a lysine residue in a given protein. (indiaeducationdiary.in)
In this paper, the collaborating research teams have identified and characterized a new methylation event, catalyzed by the methyltransferase SETD6, on the transcription factor BRD4. (indiaeducationdiary.in)
BRD4 methylation determines the recruitment of the transcription factor E2F1 to selected target genes which are involved in protein generation, a molecular mechanism which facilitates the balanced expression of these genes. (indiaeducationdiary.in)
Prof. Levy's lab focuses on the study of additional protein methylation pathways such as: cell cycles, programmed cell death, DNA damage control, DNA repair, adipocytes differentiation and more. (indiaeducationdiary.in)

Protein sequencing is the practical process of determining the amino acid sequence of all or part of a protein or peptide. (wikipedia.org)
The focus of present study was to characterize antimicrobial peptide produced by probiotic cultures, Enterococcus durans DB-1aa (MCC4243), Lactobacillus plantarum Cu2-PM7 (MCC4246) and Lactobacillus fermentum Cu3-PM8 (MCC4233) against Staphylococus aureu s and E. coli . (researchsquare.com)
Peptides were characterized by a combined use of MALDI- TOF/TOF and nanoESI-IT-ETD experiments to determine not only the peptide sequence but also the composition and the position of the carbohydrate moiety. (springer.com)
As Director of the Protein Profiling at Yale Keck Biotechnology Resource Facility, I coordinated a collaboration between the Association of Biomolecular Resource Facilities (ABRF) standard proteomic research group (sPRG) and JPT Peptide Technologies to develop the ABRF cross-species SpikeMix™ peptide pool. (jpt.com)
On all of these fronts, we are in need of high quality peptide standards for assay development and protein quantification. (jpt.com)
Here, we use the MRD method to study the hydration of the cyclic peptide oxytocin and the globular protein BPTI in deeply supercooled solutions. (lu.se)

Furthermore, after having established chemical cross-linking, projects will be able to characterize binding sites within and between protein complexes. (uni-ulm.de)
We employ this AQUA (Absolute quantification) approach regularly to determine single proteins, members of small protein complexes, or in combination with 2D Gel based proteomics on a global scale. (uni-greifswald.de)
The assembly of protein complexes and compositional lipid patterning act together to endow cells with the plasticity required to maintain compositional heterogeneity with respect to individual proteins. (biomedcentral.com)

It is often desirable to know the unordered amino acid composition of a protein prior to attempting to find the ordered sequence, as this knowledge can be used to facilitate the discovery of errors in the sequencing process or to distinguish between ambiguous results. (wikipedia.org)

2-dimensional electrophoresis (2-DE) is a powerful technique for separating proteins by isoelectric point and molecular weight. (the-scientist.com)
In this webinar sponsored by MilliporeSigma, scientists will introduce how 2-DE can be fully automated using the Auto2D® Electrophoresis Device, improving speed, protein separation, and reproducibility. (the-scientist.com)
For absolute quantification, we have combined two well-known and widely applied proteomic techniques, the separation of proteins via 2D gel electrophoresis and the absolute quantification of peptides by AQUA based on a Triple Quadrupole. (uni-greifswald.de)

An average of 1.2 proteins per gene was observed, indicating extensive post-translational regulation. (nih.gov)
With 35,000 genes and hundreds of thousands of protein states to identify, correlate, and understand, it no longer suffices to rely on studies of one gene, gene product, or process at a time. (genomicglossaries.com)
Here we report the temporal measurements of absolute RNA and protein levels per gene within a mixed bacterial-archaeal consortium. (nature.com)
We are also examining the role of the hypusine modification on eIF5A and the role of this factor in gene-specific translational control mechanisms. (nih.gov)
A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)

Bennett SA, Tanaz R, Cobos SN, Torrente MP^.Epigenetics in amyotrophic lateral sclerosis: a role for histone post-translational modifications in neurodegenerative disease. (cuny.edu)
The impact of histone post-translational modifications in neurodegenerative diseases. (cuny.edu)
As many of these neurodegenerative disorders are characterized by the presence of pathologic forms of tau and neurofibrillary tangles, studying the effects of tau pathology on the development of epilepsy might prove rewarding. (uantwerpen.be)

Bacteriocins from LAB are low molecular peptides which vary in their size, post-translation modifications, stability and mode of action (Amortegui et al. (researchsquare.com)

Recombinant Human IL18RAP protein(Phe 20 - Arg 356), fused with His tag and Avi tag, was expressed in HEK293. (creativebiomart.net)
About 70% of all recombinant therapeutic proteins are produced in mammalian cell lines, and CHO is the dominant choice for antibody production. (creativelab.hu)
To study expression of the antigenically active polypeptide products for recombinant proteins rp30, rp54 and rCD2v in the eukaryotic cells, we transfected human embryonic kidney cells HEK293T, which stably express the SV40 large T antigen, with recombinant plasmids pCI-neo/ASFV/p30, pCI-neo/ASFV/p54 and pCI-neo/ASFV/CD2v. (agrobiology.ru)
By immunoblotting, the polypeptides of the expressed recombinant proteins were identified in the HEK293T cell lysates and characterized for their molecular weights. (agrobiology.ru)
These genetic constructions will be helpful to investigate antigenic, immunogenic and protective properties of ASFV recombinant proteins rp30, rp54 and rCD2v. (agrobiology.ru)

My additional independent research on ER localisation and splicing in auxin biosynthesis showed for the first time ER-localisation for an auxin biosynthetic protein. (brookes.ac.uk)

2 − 6 These nuclease-resistant polymers persist in live organisms for days and are typically designed to recognize 25-base sequences that span intron-exon junctions or translational start sites. (cdc.gov)

The conserved family of Transcription Intermediary Factors (TIF1) proteins consists of key transcriptional regulators that control transcription of target genes by modulating chromatin state. (elifesciences.org)
Chromatin is the complex of DNA and the histone proteins with which it associates. (promegaconnections.com)
The Mi-2/NuRD complex is a multi-subunit protein complex with enzymatic activities involving chromatin remodeling and histone deacetylation. (promegaconnections.com)

Tau protein in the brain is heterogeneous, due to alternative splice forms and post-translational modifications. (medscape.com)
Lysosomal storage diseases describe a heterogeneous group of dozens of rare inherited disorders characterized by the accumulation of undigested or partially digested macromolecules, which ultimately results in cellular dysfunction and clinical abnormalities. (medscape.com)
Splicing factor mutations are particularly prevalent in MDS, a group of heterogeneous hematological disorders characterized by defective blood stem cells and a high risk of leukemia development. (lu.se)

Protein homeostasis, or proteostasis, is simply defined as the processes by which cells control the concentration and conformation of the proteome. (dana-farber.org)
Ramified astrocytes, characterized by tau-immunopositive deposits in the proximal astrocytic processes usually localized to one side of the astrocyte. (medscape.com)
Biological processes often involve the surfaces of proteins, where the structural and dynamic properties of the aqueous solvent are modified. (lu.se)

The resulting product is monospecific and characterized by superior specificity, affinity and avidity. (enzolifesciences.com)

Finally, we showed that Bonus SUMOylation is mediated by the SUMO E3-ligase Su(var)2-10, revealing that although SUMOylation of TIF1 proteins is conserved between insects and mammals, both the mechanism and specific site of modification is different in the two taxa. (elifesciences.org)

We've characterized it comprehensively and made it publicly available," explains Schiel. (technologynetworks.com)

A number of utilities to assess the effect of the modification/mutation of a given residue on the protein stability, have also been included in this package. (cran.csiro.au)

I took up a position at Oxford Brookes University in 2012 investigating the role of reticulon proteins in ER tubulation and viral trafficking in order to develop my international reputation in ER research and advanced imaging. (brookes.ac.uk)
We also investigate stress-responsive protein kinases that phosphorylate eIF2alpha, viral regulators of these kinases, and how cellular phosphatases are targeted to dephosphorylate eIF2alpha. (nih.gov)
2016). The investigation of protective immune response against ASF virus (ASFV) enabled determination of a critical role of cellular defense mechanisms and the most important viral proteins p30, p54 and CD2v (or gp 110-140) involved (P. Gomez-Puertas t al. (agrobiology.ru)

Through the use of cell-free expression to express both PAD4 and HDAC1 proteins and E. coli expression of GST fusions of PAD4 and HDAC1, pulldown experiments confirmed by in vivo experiments that PADI4 associates with the histone deacetylase 1 (HDAC1), and the corresponding activities, associate cyclically and coordinately with the pS2 promoter during repression phases. (promegaconnections.com)

We are interested in understanding how these modifications are misregulated in tumor cells, tumor-infiltrating immune cells, and tumor-interacting stroma cells. (postdocjobs.com)
Carbonic anhydrase IX (CAIX) plays an important role in the growth and survival of tumor cells.The MORC proteins contain a CW-type zinc finger domain and are predicted to have the function of regulating transcription, but no MORC2 target genes have been identified. (promegaconnections.com)
Proteomics profiling identified the tumor necrosis factor-α-induced protein 8 (TNFAIP8) as a key component enriched in TGFß-Exo. (bvsalud.org)

In this way we hope to develop therapeutics with heavily engineered cells which should exceed the complexity and refinement possible with traditional therapeutics such as small molecules or therapeutic proteins. (ukri.org)

Many aspects regarding the molecular mechanisms of small heat shock proteins, both in health and disease, remain to be unraveled. (uantwerpen.be)
Recent evidence obtained in mouse models shows its essential role regulating blood cell function through various mechanisms that include pseudohypoxia responses by hypoxia-inducible factor-1α activation, post-translational modifications like succinylation, and communication mediated by succinate receptor 1. (haematologica.org)

Parkinson's Disease (PD) associated state of neuroinflammation due to the aggregation of aberrant proteins is widely reported. (biorxiv.org)
2020). TRIM11 prevents and reverses protein aggregation and rescues a mouse model of Parkinson's disease. (cuny.edu)
The increased spectral resolution and sensitivity of the 1.2 GHz NMR has already enabled research teams to look more deeply at proteins and better understand the initial steps of amyloid-type protein aggregation as well as the function and structure of the Tau protein, both commonly associated with Alzheimer's disease. (bruker.com)

These proteins share a core structure, the conserved G-box (GDP/GTP) binding domain, of approximately 170 residues [ 3 ]. (oncotarget.com)
In addition, functions to analyze sequence environments around modifiable residues in proteins can be found. (cran.csiro.au)
For instance, 'ptm' allows to search for amino acids either overrepresented or avoided around the modifiable residues from the proteins of interest. (cran.csiro.au)

Our analysis of this data reveals an absolute protein-to-RNA ratio of 10 2 -10 4 for bacterial populations and 10 3 -10 5 for an archaeon, which is more comparable to Eukaryotic representatives' humans and yeast. (nature.com)
Indeed, the protein-to-RNA ratio per sample of the bacterial populations matched previous calculations for the existing example from axenically cultured E. coli 2 . (nature.com)
Studies show that less than 50 % of bacterial proteins and less than 15 % of non-bacterial proteins can expressed in soluble form. (creativelab.hu)

The transcription of COX-1 yields a 2.7-kilobase (kb) messenger ribonucleic acid (mRNA) that encodes a 576-residue, 65-kd protein. (medscape.com)
Conversely, the transcription of COX-2 yields a 4.5-kb mRNA that encodes a 70-kd protein with roughly 70-75% homology to the COX-1 protein. (medscape.com)

In many bacteria, mitochondria, and archaea this heme attachment is catalyzed by the cytochrome c maturation (Ccm) proteins. (ox.ac.uk)
Here we identify and characterize a covalent, ternary complex between the heme chaperone CcmE, heme, and cytochrome c. (ox.ac.uk)

The biological importance of intrinsically disordered proteins (IDPs) has been discovered in recent years by sequence-specific assignments using NMR. (bruker.com)

RT-qPCR, immunohistochemistry, and single-cell RNA sequencing characterize IL18RAP expression levels and regulation in diverse cellular contexts. (creativebiomart.net)
While the field of microbiology has adapted to the study of complex microbiomes via modern meta-omics techniques, we have not updated our basic knowledge regarding the quantitative levels of DNA, RNA and protein molecules within a microbial cell, which ultimately control cellular function. (nature.com)
For example, when Escherichia coli is grown axenically in steady state, we can expect that each RNA molecule corresponds to 10 2 -10 4 of the matching protein (absolute protein-to-RNA ratio, hereafter referred protein-to-RNA ratio) and the variation in the level of cellular RNA explains ~29% of the variation in the amount of detectable protein 2 . (nature.com)

However, there are no studies that address these questions in a realistic picture of the cell where not only proteins are present but also other elements involved in cell regulation such as DNA, RNA, chemicals or other metabolites. (europa.eu)

Herein, we developed a facile synthetic strategy to afford the sulfated hirudins with up to three modifications and in multi-milligram scales, after a single HPLC purification step. (ccspublishing.org.cn)

Rapid and comprehensive analysis of putative proteinous allergens ( allergenome ) by applying such a proteomic strategy … With allergenomics, we can not only detect and assign the putative allergens (proteins specifically interacting with IgE antibodies in a patient's blood) in a short time, but also analyze the quantitative and qualitative change of the antigens, depending on the surroundings and environmental conditions of an allergenic causative. (genomicglossaries.com)
We aim at determining the molecular targets of these modifications both by very specific approaches employing Top- down proteomic techniques based on 2D gels and by large scale enrichment techniques (e.g. (uni-greifswald.de)

Typically, partial sequencing of a protein provides sufficient information (one or more sequence tags) to identify it with reference to databases of protein sequences derived from the conceptual translation of genes. (wikipedia.org)

Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. (abcam.com)
The enriched molecular functions are mainly related to copper ion binding, molecular function regulators, protein-containing complex binding, identical protein binding and signalling receptor binding. (bvsalud.org)
GPR91 is a G protein-coupled cell surface receptor for extracellular succinate (Sucnr1). (haematologica.org)

NMR has evolved into a powerful technique in structural biology for studying the dynamics of proteins, including IDPs. (bruker.com)
The underlying structural changes, associated to the formation of these large mutant oligomers, were so far poorly characterized. (uantwerpen.be)

Together, the partners intend to develop lipocalins that can mimic antibodies and bind to sites either on other proteins or on small molecules. (genengnews.com)

Hence, the applications for imaging protein localization and dynamics require high accuracy, particularly at high spatio-temporal level. (biomedcentral.com)
We provided experimental data for the applications of Variable-Angle Epifluorescence Microscopy (VAEM) in dissecting protein dynamics in plant cells. (biomedcentral.com)
The present study presented experimental evidence on illustrating the use of VAEM in tracking and dissecting protein dynamics, dissecting endosomal dynamics, cell structure assembly along with membrane microdomain and protein motility in intact plant cells. (biomedcentral.com)
Information about the dynamics of protein hydration can be obtained by measuring the magnetic relaxation dispersion (MRD) of the water 2H and 17O nuclei or by recording the nuclear Overhauser effect (NOE) between water and protein protons. (lu.se)
Most proteins and other biomolecules have been adapted by dynamics has come from magnetic relaxation experiments using evolution to function optimally in aqueous environments. (lu.se)
In the present work, we examine to what extent complicated, being dependent on both the dynamics and the the dynamics of the hydration layer differs from that of bulk spatial proximity of water molecules to protein protons with water. (lu.se)
effect on protein energetics and dynamics. (lu.se)

Small G-proteins, monomeric GTPases, or the RAS (Rat sarcoma) superfamily are a large family of small guanine nucleotide-binding proteins with molecular weights ranging from 20 to 30 kDa [ 1 , 2 ]. (oncotarget.com)

Unlike folded proteins, IDPs are characterized by a high degree of disorder, local mobility and high dynamicity. (bruker.com)
Until recently however, it has been difficult if not impossible to select and expand T-cell populations specific for more common cancers since cancer cells, unlike virally infected cells do not contain foreign proteins. (ukri.org)

In this thesis we found that mammalian small heat shock proteins are targeted to the mitochondrial intermembrane space. (uantwerpen.be)
While small heat shock proteins of plants are targeted to virtually every membrane-enclosed compartment, this is the first example of mammalian small heat shock proteins to have an organelle-localization. (uantwerpen.be)

A generalized method often referred to as amino acid analysis for determining amino acid frequency is as follows: Hydrolyse a known quantity of protein into its constituent amino acids. (wikipedia.org)
The course aims to provide basic knowledge in bioinformatics, namely the theories and practical applications of computer-based methods for the analysis of DNA and protein sequences as well as for studies of protein structures. (liu.se)
Besides direct overlap of dual-color fluorescence signals, the co-localization analysis was carried out quantitatively in combination with the methodology for calculating puncta distance and protein proximity index. (biomedcentral.com)
The analysis indicates that water-protein NOEs are dominated by long-range dipolar couplings to bulk water, unless the monitored protein proton is near a partly or fully buried hydration site where the water molecule has a long residence time. (lu.se)

The following references illustrate the use of cell-free expression to characterize this process. (promegaconnections.com)

This company develops lipocalins, which are endogenous low-molecular-weight human proteins typically found in blood plasma and other body fluids. (genengnews.com)

Computer-based methods for analyzing DNA and protein sequences and for studying the structure of proteins. (liu.se)

The ubiquitin system in particular is critical in controlling the concentration of proteins. (dana-farber.org)

Anatomy17

Organisms8

Chemicals and Drugs122

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes73

Disciplines and Occupations5

Information Science4

Health Care1

Post-translationa12

Phosphorylation5

Characterization4

Membrane proteins5

Mass spectrometry5

PTMs8

Acetylation1

Glycosylation1

Interactions11

Synthesis7

Functional3

Degradation2

Host cell proteins2

Endoplasmic reticulum1

Regulatory3

Isoforms1

Vitro3

Methylation4

Peptide6

Complexes3

Ordered sequence1

Electrophoresis3

Gene5

Neurodegenerative3

Peptides1

Recombinant5

Biosynthesis1

Organisms1

Chromatin3

Heterogeneous3

Processes3

Specificity1

Mechanism1

Comprehensively1

Stability1

Viral3

Histone1

Tumor3

Therapeutic1

Mechanisms2

Aggregation3

Residues3

Bacterial3

MRNA2

Heme2

Biological1

Cellular3

Regulation1

Purification1

Proteomic2

Databases1

Receptor3

Structural2

Antibodies1

Dynamics7

GTPases1

Unlike2

Mammalian2

Analysis4

Cell1

Typically1

Sequences1

Concentration1