• Our study has highlighted sex-specific alterations in systolic and diastolic function shortly after ischemia, and provided a comprehensive look at the underlying proteomic changes and the influence of estrogens and their metabolites. (biomedcentral.com)
  • The differential proteins were mainly related to extracellular components, proteasome, and metabolism. (selectbiosciences.com)
  • SVEVs isolated from lyophilized venoms collected from four different species of snakes ( Agkistrodon contortrix contortrix , Crotalus atrox , Crotalus viridis and Crotalus cerberus oreganus ) were analyzed by mass spectrometry-based proteomic, which allowed the identification of proteins belonging to eight main functional protein classes such as SVMPs, serine proteinases, PLA 2 , LAAO, 5′nucleotidase, C-type lectin, CRISP and Disintegrin. (nature.com)
  • 2224 proteins were quantified, with 520 showing sex-specific differential regulation. (biomedcentral.com)
  • Many proteins are discovered on an annual basis and in the field of drug discovery it is very important characterize the structure of a novel peptides as well as the best conditions to keep them in solution. (wikipedia.org)
  • P. aeruginosa responded to spaceflight conditions through differential regulation of 167 genes and 28 proteins, with Hfq as a global transcriptional regulator. (sckcen.be)
  • In this study, we characterized glycoproteins and nonglycoproteins on pooled mucinous (n = 10) and nonmucinous (n = 10) pancreatic cyst fluid to identify "proteins of interest" to differentiate between mucinous cyst from nonmucinous cyst and investigate these proteins as potential biomarker targets. (nih.gov)
  • Spectral count quantitation allowed for the identification of proteins with significant differential levels in mucinous cysts from nonmucinous cysts of which one protein (periostin) was confirmed via immunoblotting. (nih.gov)
  • To exhaustively evaluate differentially expressed proteins, we used a number of proteomic tools including gene ontology classification, pathway and network analysis, Novoseek data mining, and chromosome gene mapping. (nih.gov)
  • Utilization of complementary proteomic tools revealed that several of the proteins such as mucin 6 (MUC6), bile salt-activated lipase (CEL), and pyruvate kinase lysozyme M1/M2 with significant differential expression have strong association with pancreatic cancer. (nih.gov)
  • Diana Samodova and a team at the University of Copenhagen characterized another protease, ProAlanase, isolated from the fungus Aspergillus niger , that cleaves primarily on the C-terminal side of proline and alanine amino acid residues of proteins. (asbmb.org)
  • In this study, we combined transcript profiling and proteomic analysis to better understand which genes and proteins are altered by opaque2 in the W64A inbred line. (biomedcentral.com)
  • Proteomic methodologies increasingly have been applied to the kidney to map the renal cortical proteome and to identify global changes in renal proteins induced by diseases such as diabetes. (biomedcentral.com)
  • Low coverage of the renal cortical proteome as well as our interest in diabetes-induced changes in proteins found in the renal cortex prompted us to perform an in-depth proteomic analysis of mouse renal cortical tissue. (biomedcentral.com)
  • A whole tissue extract of the mouse kidney cortex was analyzed by an unbiased proteomic approach, yielding a dataset of ~2,000 unique proteins identified with strict criteria to ensure a high level of confidence in protein identification. (biomedcentral.com)
  • Many of these proteins, such as low-abundance proteins, membrane proteins and proteins with extreme values in pI or MW are traditionally under-represented in 2-DE-based proteomic analysis. (biomedcentral.com)
  • A total of 41 of these formerly N-linked glycosite-containing peptides (corresponding to 37 proteins) were reproducibly quantified based on their relative peak area ratios in human serum during PRM assay development, with 4 proteins showing differential significance in serum from nonaggressive (NAG) vs aggressive (AG) prostate cancer patient serum (n = 50, NAG vs AG). (nih.gov)
  • In addition, proteomic profile confirmed that a set of proteins, related to tumor aggressiveness, were positively affected by targeted nanoparticles. (dovepress.com)
  • Many proteins identified in this study were identified by proteomic methods for the first time (e.g. hypothetical proteins or proteins annotated based solely on their chromosomal location). (amritabioquest.org)
  • None of the proteins showed a significant yet opposite regulation in between DLB and PDD when compared to aged controls in the proteomic data set as well as following immunoblot analysis of the pooled and individual lysates involving all 61 subjects. (biomedcentral.com)
  • Significant insight into the function of proteins can be delineated by discovering and characterizing interacting proteins. (aacrjournals.org)
  • Overall, this Lys-N/guanidination integrative approach is applicable for functional proteomic studies utilizing MALDI mass spectrometry analysis, as it provides an effective and economical mean for relative quantification of proteins in conjunction with increased sensitivity of detection and fragmentation efficiency. (princeton.edu)
  • Quantification of ~250-800 different proteins among three different types of neurons revealed reproducible proteomic differences between the neuron types. (njacs.org)
  • Following this reasoning, we performed a differential proteomic analysis in order to search for OA-related changes in human articular chondrocyte intracellular proteins, which aimed to unravel those molecular mechanisms that participate in OA pathogenesis [ 4 ]. (biomedcentral.com)
  • nov. 6c and assessed its transcriptomic and proteomic response to tissue extracts of its native host, the Red Sea coral Acropora humilis. (uni-konstanz.de)
  • 2013 . Active transcriptomic and proteomic reprogramming in the C. elegans nucleotide excision repair mutant xpa-1. (henlab.org)
  • Subsequent RNA sequence analysis and liquid chromatography with mass tandem spectrometry analyses showed that these variations translated into discretely separated transcriptomic and proteomic profiles. (cdc.gov)
  • On the basis of these observations, we hypothesized that strains with different genomic organization should display altered global transcriptomic and, consequently, proteomic profiles, and thereby genome rearrangements might contribute to strain variation and adaptation. (cdc.gov)
  • To validate this assumption, we have performed genomic, transcriptomic, and proteomic analyses of recent clinical isolates from the Czech Republic obtained during 2008-2015, previously characterized vaccine strains isolated during 1954-1965 ( 22 ) (hereafter referred to as historic strains), and the reference strain Tohama I. (cdc.gov)
  • Preeclampsia (PE) is a pregnancy associated disorder characterized by hypertension and proteinuria. (hindawi.com)
  • Chronic fatigue syndrome (CFS) is a debilitating and complex disorder characterized by unexplained fatigue not improved by rest. (unipi.it)
  • As an inherited disorder characterized by severe pulmonary disease, cystic fibrosis could be considered a comorbidity for coronavirus disease 2019. (cdc.gov)
  • This involves use of comparative genomics and proteomic approaches to understand molecular pathogenic aspects of these bacterial agents. (salmonella-systomics.ca)
  • We previously showed that P. aeruginosa expresses the Has and Phu heme uptake systems and the PVD- and PCH-dependent iron uptake pathways in iron-restricted growth conditions, using proteomic and RT-qPCR approaches. (cnrs.fr)
  • We use proteomic/peptidomic approaches in patient samples to discover novel biomarker candidates which might improve (early/differential) diagnosis, disease monitoring or drug development (e.g. (proteomics-academy.org)
  • Combining these two layers of information poses challenges to the application of traditional statistical approaches in differential expression analysis. (biomedcentral.com)
  • This review will highlight some of the recent technical advances in proteomic-based discovery approaches, the pros and cons of various methods and some of the key findings in cancer-related systems. (aacrjournals.org)
  • In this presentation, we will discuss the development of in situ/vivo approaches by cellular and subcellular mass spectrometry that enabled our lab to determine the proteomic and metabolomic profile of identified cells in live Xenopus laevis frog embryos developing to tadpoles and neurons in mouse brain tissues. (njacs.org)
  • Qian's current research involves the development of chemical proteomic approaches for site-specific quantification of cysteine-based redox modifications and more sensitive selected reaction monitoring-based targeted quantification techniques with applications in pancreatic islets, diabetes, and oxidative stress-related disease areas. (pnnl.gov)
  • Transcriptional and proteomic responses of Pseudomonas aeruginosa PAO1 to spaceflight conditions involve Hfq regulation and reveal a role for oxygen. (sckcen.be)
  • This study is the first to characterize the spaceflight-induced global transcriptional and proteomic responses of Pseudomonas aeruginosa, an opportunistic pathogen that is present in the space habitat. (sckcen.be)
  • We then apply a suite of tools, such as signature tagged mutagenesis, in vivo induced antigen technology, suppression subtractive hybridization and custom designed microarray, to characterize the differences between organisms under the broad hypothesis that the differential pathogenesis is controlled at the genetic, transcriptional, and protein level. (salmonella-systomics.ca)
  • These outcomes support the hypothesis of LDE225 common proteomic adjustments during MSCs enlargement and could provide important understanding in the data of molecular systems intervening during MSC enlargement. (sciencepop.org)
  • Here, we report a proteomic study, as part of a Systems Biology of Epilepsy Project, which utilizes in vivo electrophysiologically-characterized human brain samples from the neocortex of 6 patients with refractory epilepsy, to determine whether there are common proteomic patterns in human brain regions that produce epileptic discharges. (montana.edu)
  • To further characterize pathways involved in naphthalene degradation in NaphS2, the draft genome was sequenced, and gene and protein expression examined. (elsevierpure.com)
  • Further analysis showed that the dystrophic condition is characterized by multiple perturbed pathways and crosstalk among inflammatory, metabolic and muscle growth pathways. (asbmb.org)
  • This analysis predicted proteomic changes due to both histological differences and molecular pathways by examination of gene ontology clusters. (montana.edu)
  • Genes including the previously characterized NmsA, thought to encode an enzyme necessary for 2-methylnaphthalene metabolism, were not upregulated during growth of NaphS2 on naphthalene, nor were the corresponding protein products. (elsevierpure.com)
  • We show that coral tissue extracts stimulated differential expression of genes putatively involved in symbiosis establishment via the modulation of the host immune response by E. marisrubri 6c, such as genes for flagellar assembly, ankyrins, ephrins, and serpins. (uni-konstanz.de)
  • 2015 . The fat cell epigenetic signature in post-obese women is characterized by global hypomethylation and differential DNA methylation of adipogenesis genes. (henlab.org)
  • NaphS2 may possess a non-classical dearomatizing enzyme for benzoate degradation, similar to one previously characterized in Geobacter metallireducens. (elsevierpure.com)
  • Proteomic assessment of CCC and EC demonstrates remarkable differences in protein expression that are dependent on histotype, thereby further characterizing these cancers. (biomedcentral.com)
  • In this regard, a systematic approach to studying changes in proteomic landscape may uncover other similarities and differences between DLB and PDD. (biomedcentral.com)
  • Molecular measurements using capillary electrophoresis time-of-flight or orbitrap mass spectrometer platforms revealed proteomic and metabolomic differences between the X. laevis cells that correlated with phenotype. (njacs.org)
  • Confocal studies showed differential cellular and compartmental distribution of isozymes involved in glucose, glutamate, glutamine, lactate, and creatine metabolism. (molvis.org)
  • It is therefore of prime importance to better characterize its consequences at the cellular level. (nature.com)
  • In a recent paper in the journal Molecular & Cellular Proteomics , Tirsa L.E. van Westering and researchers at the University of Oxford used mass spectrometry to identify differential protein expression in muscles at three different ages (representing the different stages of DMD pathology) from two strains of mice with genetically altered DMD and one strain of unaltered mice (control). (asbmb.org)
  • In this scenario, proteomics helps to identify molecular mechanisms and characterize metabolic and cellular processes in several physiopathological conditions. (mdpi.com)
  • Cell-generated EV spike-in models were isolated and purified by size-exclusion chromatography, stained with cellular dyes and characterized by nano flow cytometry. (biomedcentral.com)
  • Methodology and Principal Findings: Using a proteomics differential approach with two-Dimensional Differential in-Gel Electrophoresis (2D-DIGE), we defined the protein modulations in the midgut of Aedes aegypti that were triggered seven days after an oral infection (7 DPI) with dengue 2 (DENV-2) and chikungunya (CHIKV) viruses. (hal.science)
  • While a lot of attention has been paid to the statistical analysis of genomic and proteomic data, less has been given to data from metabolomics experiments. (biomedcentral.com)
  • CB2R-IN-1 Proteomic analysis showed significant alterations of the molecular composition of subsynaptic compartments of hippocampal trisomic neurons. (logic2010.org)
  • Chondrocyte metabolism is influenced by several cytokines and growth factors, which drive two qualitatively distinct functional programs in these cells: the catabolic program is induced by proinflammatory stimuli and characterized by the secretion of proteases, suppression of matrix synthesis, and induction of chondrocyte apoptosis. (biomedcentral.com)
  • The classicalcadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series offive homologous NH2 terminal repeats. (logic2010.org)
  • Strategies: To evaluate the prospective role of extracellular vesicles (EVs) in advertising these effective effects, we isolated EVs from the tiny intestinal mucus of rodents following a sham or vertical sleeve gastrectomy (VSG) surgeries utilizing differential centrifugation. (ack1inhibitor.com)
  • Osteoarthritis (OA) is a slowly progressive degenerative disease characterized by the degradation of the extracellular matrix (ECM) and cell death, resulting in a gradual loss of articular cartilage integrity, intra-articular inflammation and changes in peri-articular and subchondral bone [ 1 ]. (biomedcentral.com)
  • Our goals were twofold: 1) to characterize the gene expression, protein expression, and activity of key synthesizing and regulating enzymes of energy metabolism in the whole mouse retina, retinal compartments, and/or cells and 2) to provide an integrative analysis of the results related to function. (molvis.org)
  • We studied the influence of histological subtype on protein expression with reverse phase protein array (RPPA) and assessed proteomic changes associated with ARID1A mutation/BAF250a expression in EC and CCC. (biomedcentral.com)
  • SAM (Significance Analysis of Microarrays) was performed to determine differential protein expression by histological subtype and ARID1A mutation status. (biomedcentral.com)
  • Hence, to explore their potential contribution to tumor progression, we characterized the proteomic composition of RBCs from 53 BC patients from stages I to III and IV, compared with 33 cancer-free controls. (selectbiosciences.com)
  • In this work, we observed that RBCs from BC patients showed a different proteomic profile compared to cancer-free controls and between different tumor stages. (selectbiosciences.com)
  • Seemingly, RBCs are acquiring modifications in their proteomic composition that probably represents the systemic cancer disease, conditioned by the tumor microenvironment. (selectbiosciences.com)
  • Each assay was characterized by its performance metrics and criteria established by the National Cancer Institute's Clinical Proteomic Tumor Analysis Consortium (NCI CPTAC) to facilitate the widespread adoption of the assays in studies designed to confidently detect changes in the relative abundance of these analytes. (nih.gov)
  • This study highlights the importance of investigating alternative proteases for proteomic experiments. (asbmb.org)
  • RESULTS: Draft genome sequencing, gene expression analysis, and proteomic analysis revealed that NaphS2 degrades naphthoyl-CoA in a manner analogous to benzoyl-CoA degradation. (elsevierpure.com)
  • In this paper, we focus on differential expression analysis of MS-based metabolomics data. (biomedcentral.com)
  • A big challenge in metabolomics differential expression analysis is that the data matrix frequently contains missing values. (biomedcentral.com)
  • Although non-CYP enzymes are expressed in multiple human tissues, differential tissue expression and interindividual variability in the expression of these enzymes are not well studied. (njacs.org)
  • Discovering Common Pathophysiological Processes between COVID-19 and Cystic Fibrosis by Differential Gene Expression Pattern Analysis. (cdc.gov)
  • To identify these potential molecular changes, in this study, we have optimized a biochemical subfractioning method permitting the purification of subsynaptic compartments (pre-, extra-, and postsynaptic fractions) for further proteomic and phosphoproteomic analysis of Ts65Dn adult mice hippocampi. (logic2010.org)
  • We made use of molecular biology, genetics, microscopy and mass spectrometry-based technologies to characterize the EVs from every single supply.ISEV 2018 abstract bookRes. (ack1inhibitor.com)
  • The mathematics will link macro scale biological observations to micro scale molecular movements to characterise the relative role that different components and processes play. (edu.au)
  • They are characterized by the presence of cortical α-synuclein-positive Lewy bodies (LBs) and Parkinsonian extrapyramidal motor symptoms. (biomedcentral.com)
  • The human placenta is a difficult tissue to work with using proteomic technology since it contains large amounts of lipids and glycogen. (hindawi.com)
  • We aimed to investigate the early, sex-specific functional and proteomic changes following myocardial ischemia in an unbiased approach. (biomedcentral.com)
  • Concentrations of circulating steroid hormones were measured with LC-MS/MS. Feature selection (PLS and PLS-DA) was used to examine associations among functional, proteomic and hormonal datasets. (biomedcentral.com)
  • Bioinformatics highlighted possible associations of estrogens and their metabolites with early functional and proteomic alterations. (biomedcentral.com)
  • The differential clinical value carried within each distinct EV subset highlights the advantages of selective isolation. (biomedcentral.com)
  • Although a similar clinical proteomic approach has been used to study Parkinson's disease (PD), few studies have focused on Lewy body dementias exclusively [ 21 ]. (biomedcentral.com)
  • Taking advantage of this improved fragmentation pattern, we utilized differential 15 N/ 14 N guanidination of Lys-N peptides and MALDI-MS/MS analysis to relatively quantify the changes in PAP I associations due to deletion of sprE, previously shown to regulate PAP I-dependent polyadenylation. (princeton.edu)
  • Phosphoenrichment-coupled mass spectrometry analysis revealed specific subsynaptic- and trisomy-associated iGluR phosphorylation signature, concomitant with differential subsynaptic kinase and phosphatase composition of Ts65Dn hippocampal subsynaptic compartments. (logic2010.org)
  • Solutions: Circulating MPs of non-MetS subjects and MetS sufferers happen to be isolated from plasma and characterized by ERK1 Activator custom synthesis proteomic evaluation. (ack1inhibitor.com)
  • It will offer many researchers a wider perspective into the proteomic signatures characteristic of DMD and potential therapeutic targets and also will be useful for researchers studying the effects of aging on the muscle proteome. (asbmb.org)
  • 2023. scDual-Seq of Toxoplasma gondii-infected mouse BMDCs reveals heterogeneity and differential infection dynamics. (henlab.org)
  • 3. Use single deletion mutants of S. enterica to further characterize the interaction of the human pathogen with the plant basal plant defense system. (salmonella-systomics.ca)
  • 2021. "Proteomic Profiling of the Substantia Nigra to Identify Determinants of Lewy Body Pathology and Dopaminergic Neuronal Loss. (pnnl.gov)
  • Recent work has shown that using a metalloendopeptidase, Lys-N, for proteomic analysis of biological protein mixtures produces complementary protein sequence information compared with trypsin digestion alone. (princeton.edu)
  • We introduce PCprophet, a toolkit built on size exclusion chromatography-sequential window acquisition of all theoretical mass spectrometry (SEC-SWATH-MS) data to predict protein complexes and characterize their changes across experimental conditions. (lu.se)
  • Proteomic patterns of cells ready were in keeping with 4 up-regulated peaks (mass-to-charge percentage (m/z) 8950 10087 10345 and 13058) through subculture measures (P0-P7) with identical craze in three donors. (sciencepop.org)
  • We discovered that long term tradition significantly modified the AD-MSC proteomic phenotype with lots of the observed peaks. (sciencepop.org)
  • Protein spots were resolved from three subcellular fractions, using two-dimensional differential-in-gel-electrophoresis, revealing 31 spots that changed significantly and were identified by liquid-chromatography tandem mass-spectrometry. (montana.edu)
  • Proteomic Analysis of Human Sputum for the Diagnosis of Lung Disorders: Where Are We Today? (cdc.gov)
  • The association of the Oeckl lab to the Neurology Department of the Ulm University Hospital provides excellent interaction with neurologists and psychologists and access to well-characterized patient samples enabling highly clinically-oriented research. (proteomics-academy.org)
  • Such screens include co-factors, substrates, differing protein or nucleotide partners or small molecule inhibitors, to more fully characterize the variations within assembly states and key conformational changes. (amritabioquest.org)
  • To characterize specific biomarker candidates and the related pathomechanisms in detail (e.g. synaptic marker beta-synuclein in blood), we develop highly sensitive and accurate methods using targeted mass spectrometry (MRM, PRM, IP-MS) to study samples from large patient cohorts and evaluate their value for use in the clinic or drug development (e.g. (proteomics-academy.org)
  • Duchenne muscular dystrophy, or DMD, is characterized by progressive muscle degeneration and weakness due to the alterations of the dystrophin protein that keeps muscles intact. (asbmb.org)
  • The immunoreactivity levels of well-characterized antibodies, for all major retinal cells and their compartments, were obtained using our established semiquantitative confocal and imaging techniques. (molvis.org)
  • For example, mass spectrometry (MS)-based metabolomics data is typically characterized by high dimensionality, small sample size, high correlation structure between metabolites, redundant information (such as adducts and fragments), and especially the sparse data matrix, which is comprised of the samples, the variable ID (m/z, retention time), and peak area. (biomedcentral.com)
  • ARID1A mutations were identified by exome sequencing, and PIK3CA mutations were characterized by MALDI-TOF mass spectrometry. (biomedcentral.com)
  • Here, we have investigated the suitability of Lys-N proteolysis for use with MALDI mass spectrometry to characterize the yeast Arp2 complex and E. coli PAP I protein interactions. (princeton.edu)
  • Direct comparison of Dll1- and Dll4-mediated Notch activation levels shows differential lymphomyeloid lineage commitment outcomes. (sunnybrook.ca)
  • A proteomic approach was undertaken to characterize the key virus/vector interactions and host protein modifications that happen in the midgut for viral transmission to eventually take place. (hal.science)
  • Results: Differential proteomic evaluation of MPs from each varieties of people identifies Rap1, a tiny GTPase, as twofold overexpressed in MPs from MetS compared with non-MetS subjects. (ack1inhibitor.com)
  • In this specific article we describe the usage of SELDI-ToF technology to judge the proteomic profile of AD-MSC and examine how this profile adjustments during growth. (sciencepop.org)
  • In a previous work, we investigated the proteomic salivary profile in a couple of monozygotic twins discordant for CFS. (unipi.it)
  • With this context the necessity of rigorous specifications for characterizing human being MSCs is quite crucial and many studies are ongoing to attain this goal. (sciencepop.org)